MypaA.00414.c
Putative short-chain dehydrogenase or 3-oxoacyl-[acyl-carrier-protein] reductase
| CENTER ID: | MypaA.00414.c |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00414.c.A1.GE29448 | Full length( MypaA.00414.c ) | 1 | 249 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00414.c.A1.PS00733 | Full length( MypaA.00414.c ) | 1 | 249 |
Structures
3QIV
DEPOSITED: 1/27/2011
DETERMINATION: XRay
CLONE: MypaA.00414.c.A1.GE29448
PROTEIN: MypaA.00414.c.A1.PS00733
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_169253 |
| PATRIC ID: | fig|262316.17.peg.639 |
| RefSeq: | NP_959537.1 |
| UniProt: | Q743I2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFENKVGIV TGSGGGIGQA YAEALAREGA AVVVADINAE AAEAVAKQIV ADGGTAISVA VDVSDPESAK AMADRTLAEF GGIDYLVNNA AIFGGMKLDF LLTIDPEYYK KFMSVNLDGA LWCTRAVYKK MTKRGGGAIV NQSSTAAWLY SNYYGLAKVG INGLTQQLSR ELGGRNIRIN AIAPGPIDTE ANRTTTPKEM VDDIVKGLPL SRMGTPDDLV GMCLFLLSDE ASWITGQIFN VDGGQIIRS
NT Sequence
ATGCGATTCG AGAACAAAGT CGGCATCGTC ACCGGATCCG GGGGCGGCAT CGGGCAGGCG TACGCCGAGG CGCTGGCCCG CGAGGGCGCG GCGGTGGTGG TCGCCGACAT CAACGCCGAG GCGGCCGAGG CCGTCGCCAA ACAGATTGTC GCCGACGGCG GTACGGCCAT CAGCGTCGCG GTCGACGTGT CCGACCCCGA GTCGGCCAAG GCGATGGCCG ACCGCACCCT GGCCGAATTC GGCGGCATCG ACTACCTGGT CAACAACGCC GCGATCTTCG GGGGCATGAA GCTGGACTTT TTGCTCACCA TCGACCCGGA GTACTACAAG AAATTCATGA GCGTGAACCT CGACGGGGCG CTGTGGTGCA CCCGCGCGGT GTACAAGAAG ATGACCAAGC GCGGCGGCGG GGCGATCGTC AACCAGTCGT CCACCGCGGC CTGGCTGTAT TCGAACTACT ACGGCCTGGC CAAGGTCGGC ATCAACGGCC TGACCCAGCA GCTGTCCCGC GAACTGGGCG GGCGCAACAT CCGGATCAAC GCGATCGCGC CCGGGCCCAT CGACACCGAG GCCAACCGGA CCACCACACC CAAAGAGATG GTCGACGACA TCGTCAAGGG ACTTCCGTTG TCGCGCATGG GAACTCCCGA CGACCTGGTC GGCATGTGCC TGTTCCTGCT GTCCGACGAG GCGTCGTGGA TCACCGGGCA GATCTTCAAC GTCGACGGCG GGCAGATAAT CCGATCA
Details for MypaA.00414.c.A1.GE29448
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRFENKVGI VTGSGGGIGQ AYAEALAREG AAVVVADINA EAAEAVAKQI VADGGTAISV AVDVSDPESA KAMADRTLAE FGGIDYLVNN AAIFGGMKLD FLLTIDPEYY KKFMSVNLDG ALWCTRAVYK KMTKRGGGAI VNQSSTAAWL YSNYYGLAKV GINGLTQQLS RELGGRNIRI NAIAPGPIDT EANRTTTPKE MVDDIVKGLP LSRMGTPDDL VGMCLFLLSD EASWITGQIF NVDGGQIIRS
Validated NT Sequence
ttattatgat cggattatct gcccgccgtc gacgttgaag atctgcccgg tgatccacga cgcctcgtcg gacagcagga acaggcacat gccgaccagg tcgtcgggag ttcccatgcg cgacaacgga agtcccttga cgatgtcgtc gaccatctct ttgggtgtgg tggtccggtt ggcctcggtg tcgatgggcc cgggcgcgat cgcgttgatc cggatgttgc gcccgcccag ttcgcgggac agctgctggg tcaggccgtt gatgccgacc ttggccaggc cgtagtagtt cgaatacagc caggccgcgg tggacgactg gttgacgatc gccccgccgc cgcgcttggt catcttcttg tacaccgcgc gggtgcacca cagcgccccg tcgaggttca cgctcatgaa tttcttgtag tactccgggt cgatggtgag caaaaagtcc agcttcatgc ccccgaagat cgcggcgttg ttgaccaggt agtcgatgcc gccgaattcg gccagggtgc ggtcggccat cgccttggcc gactcggggt cggacacgtc gaccgcgacg ctgatggccg taccgccgtc ggcgacaatc tgtttggcga cggcctcggc cgcctcggcg ttgatgtcgg cgaccaccac cgccgcgccc tcgcgggcca gcgcctcggc gtacgcctgc ccgatgccgc ccccggatcc ggtgacgatg ccgactttgt tctcgaatcg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRFENKVGI VTGSGGGIGQ AYAEALAREG AAVVVADINA EAAEAVAKQI VADGGTAISV AVDVSDPESA KAMADRTLAE FGGIDYLVNN AAIFGGMKLD FLLTIDPEYY KKFMSVNLDG ALWCTRAVYK KMTKRGGGAI VNQSSTAAWL YSNYYGLAKV GINGLTQQLS RELGGRNIRI NAIAPGPIDT EANRTTTPKE MVDDIVKGLP LSRMGTPDDL VGMCLFLLSD EASWITGQIF NVDGGQIIRS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgattcgag aacaaagtcg gcatcgtcac cggatccggg ggcggcatcg ggcaggcgta cgccgaggcg ctggcccgcg agggcgcggc ggtggtggtc gccgacatca acgccgaggc ggccgaggcc gtcgccaaac agattgtcgc cgacggcggt acggccatca gcgtcgcggt cgacgtgtcc gaccccgagt cggccaaggc gatggccgac cgcaccctgg ccgaattcgg cggcatcgac tacctggtca acaacgccgc gatcttcggg ggcatgaagc tggacttttt gctcaccatc gacccggagt actacaagaa attcatgagc gtgaacctcg acggggcgct gtggtgcacc cgcgcggtgt acaagaagat gaccaagcgc ggcggcgggg cgatcgtcaa ccagtcgtcc accgcggcct ggctgtattc gaactactac ggcctggcca aggtcggcat caacggcctg acccagcagc tgtcccgcga actgggcggg cgcaacatcc ggatcaacgc gatcgcgccc gggcccatcg acaccgaggc caaccggacc accacaccca aagagatggt cgacgacatc gtcaagggac ttccgttgtc gcgcatggga actcccgacg acctggtcgg catgtgcctg ttcctgctgt ccgacgaggc gtcgtggatc accgggcaga tcttcaacgt cgacggcggg cagataatcc gatcaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MypaA.00414.c.A1.PS00733
| PURIFICATION DATe: | 8/20/2010 |
| CONCENTRATION: | 16.94mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRFENKVGI VTGSGGGIGQ AYAEALAREG AAVVVADINA EAAEAVAKQI VADGGTAISV AVDVSDPESA KAMADRTLAE FGGIDYLVNN AAIFGGMKLD FLLTIDPEYY KKFMSVNLDG ALWCTRAVYK KMTKRGGGAI VNQSSTAAWL YSNYYGLAKV GINGLTQQLS RELGGRNIRI NAIAPGPIDT EANRTTTPKE MVDDIVKGLP LSRMGTPDDL VGMCLFLLSD EASWITGQIF NVDGGQIIRS
Validated NT Sequence
ttattatgat cggattatct gcccgccgtc gacgttgaag atctgcccgg tgatccacga cgcctcgtcg gacagcagga acaggcacat gccgaccagg tcgtcgggag ttcccatgcg cgacaacgga agtcccttga cgatgtcgtc gaccatctct ttgggtgtgg tggtccggtt ggcctcggtg tcgatgggcc cgggcgcgat cgcgttgatc cggatgttgc gcccgcccag ttcgcgggac agctgctggg tcaggccgtt gatgccgacc ttggccaggc cgtagtagtt cgaatacagc caggccgcgg tggacgactg gttgacgatc gccccgccgc cgcgcttggt catcttcttg tacaccgcgc gggtgcacca cagcgccccg tcgaggttca cgctcatgaa tttcttgtag tactccgggt cgatggtgag caaaaagtcc agcttcatgc ccccgaagat cgcggcgttg ttgaccaggt agtcgatgcc gccgaattcg gccagggtgc ggtcggccat cgccttggcc gactcggggt cggacacgtc gaccgcgacg ctgatggccg taccgccgtc ggcgacaatc tgtttggcga cggcctcggc cgcctcggcg ttgatgtcgg cgaccaccac cgccgcgccc tcgcgggcca gcgcctcggc gtacgcctgc ccgatgccgc ccccggatcc ggtgacgatg ccgactttgt tctcgaatcg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRFENKVGI VTGSGGGIGQ AYAEALAREG AAVVVADINA EAAEAVAKQI VADGGTAISV AVDVSDPESA KAMADRTLAE FGGIDYLVNN AAIFGGMKLD FLLTIDPEYY KKFMSVNLDG ALWCTRAVYK KMTKRGGGAI VNQSSTAAWL YSNYYGLAKV GINGLTQQLS RELGGRNIRI NAIAPGPIDT EANRTTTPKE MVDDIVKGLP LSRMGTPDDL VGMCLFLLSD EASWITGQIF NVDGGQIIRS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGATTCGAG AACAAAGTCG GCATCGTCAC CGGATCCGGG GGCGGCATCG GGCAGGCGTA CGCCGAGGCG CTGGCCCGCG AGGGCGCGGC GGTGGTGGTC GCCGACATCA ACGCCGAGGC GGCCGAGGCC GTCGCCAAAC AGATTGTCGC CGACGGCGGT ACGGCCATCA GCGTCGCGGT CGACGTGTCC GACCCCGAGT CGGCCAAGGC GATGGCCGAC CGCACCCTGG CCGAATTCGG CGGCATCGAC TACCTGGTCA ACAACGCCGC GATCTTCGGG GGCATGAAGC TGGACTTTTT GCTCACCATC GACCCGGAGT ACTACAAGAA ATTCATGAGC GTGAACCTCG ACGGGGCGCT GTGGTGCACC CGCGCGGTGT ACAAGAAGAT GACCAAGCGC GGCGGCGGGG CGATCGTCAA CCAGTCGTCC ACCGCGGCCT GGCTGTATTC GAACTACTAC GGCCTGGCCA AGGTCGGCAT CAACGGCCTG ACCCAGCAGC TGTCCCGCGA ACTGGGCGGG CGCAACATCC GGATCAACGC GATCGCGCCC GGGCCCATCG ACACCGAGGC CAACCGGACC ACCACACCCA AAGAGATGGT CGACGACATC GTCAAGGGAC TTCCGTTGTC GCGCATGGGA ACTCCCGACG ACCTGGTCGG CATGTGCCTG TTCCTGCTGT CCGACGAGGC GTCGTGGATC ACCGGGCAGA TCTTCAACGT CGACGGCGGG CAGATAATCC GATCAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA