MymaA.00414.b

Dehydrogenase/reductase

CENTER ID: MymaA.00414.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00414.b.A1.GE30020 Full length( MymaA.00414.b ) 1 250
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00414.b.A1.PS00821 Full length( MymaA.00414.b ) 1 250

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5280
RefSeq: YP_001853186.1
UniProt: B2HH76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGQFENKVAI VTGAAQGIGQ AYADALAREG ARVVVADINA EAAEKTAKQI VADGGTAIHV PVDVSDEESA RAMADRTVSE FGGIDYLVNN AAIYGGMKLD LLLTVPLDYY KKFMSVNHDG VLVCTRAVYK HMAKRGGGAI VNQSSTAAWL YSNFYGLAKV GVNGLTQQLS RELGGMKIRI NAIAPGPIDT EATRTVTPGE FVKDMVKQIP LSRMGTPEDL VGMCLFLLGD QASWITGQIF NVDGGQIIRS
NT Sequence
GTGGGACAGT TCGAGAACAA GGTGGCGATC GTCACCGGGG CGGCGCAGGG TATTGGCCAG GCGTATGCGG ACGCCCTGGC TCGCGAGGGT GCGCGCGTGG TCGTCGCCGA CATCAATGCC GAGGCCGCCG AGAAGACCGC AAAGCAGATT GTCGCCGACG GTGGCACGGC AATTCATGTG CCCGTGGATG TTTCCGATGA GGAGTCGGCC AGGGCGATGG CCGATCGTAC GGTCAGCGAG TTCGGCGGCA TCGACTACCT GGTGAACAAC GCCGCGATCT ACGGCGGCAT GAAGCTCGAC CTACTGCTGA CGGTGCCGTT GGATTACTAC AAGAAGTTCA TGAGCGTCAA CCACGACGGG GTGCTGGTGT GCACGCGCGC CGTGTACAAG CACATGGCCA AACGCGGTGG CGGGGCAATC GTGAACCAGT CGTCGACGGC GGCGTGGCTG TACTCGAACT TCTATGGTTT GGCCAAAGTC GGGGTCAACG GGCTTACCCA ACAGCTTTCC CGTGAGCTTG GCGGAATGAA GATCCGGATC AATGCGATCG CGCCGGGACC GATCGACACC GAAGCCACCA GAACCGTCAC GCCCGGCGAG TTCGTCAAGG ACATGGTCAA GCAGATCCCG TTGAGCCGGA TGGGGACTCC CGAGGACCTG GTGGGGATGT GCCTGTTCTT GCTCGGCGAC CAGGCCTCCT GGATTACCGG ACAGATCTTC AATGTCGATG GCGGCCAGAT CATCCGCTCG
Details for MymaA.00414.b.A1.GE30020
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 28kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGQFENKV AIVTGAAQGI GQAYADALAR EGARVVVADI NAEAAEKTAK QIVADGGTAI HVPVDVSDEE SARAMADRTV SEFGGIDYLV NNAAIYGGMK LDLLLTVPLD YYKKFMSVNH DGVLVCTRAV YKHMAKRGGG AIVNQSSTAA WLYSNFYGLA KVGVNGLTQQ LSRELGGMKI RINAIAPGPI DTEATRTVTP GEFVKDMVKQ IPLSRMGTPE DLVGMCLFLL GDQASWITGQ IFNVDGGQII R
Validated NT Sequence
ttattagcgg atgatctggc cgccatcgac attgaagatc tgtccggtaa tccaggaggc ctggtcgccg agcaagaaca ggcacatccc caccaggtcc tcgggagtcc ccatccggct caacgggatc tgcttgacca tgtccttgac gaactcgccg ggcgtgacgg ttctggtggc ttcggtgtcg atcggtcccg gcgcgatcgc attgatccgg atcttcattc cgccaagctc acgggaaagc tgttgggtaa gcccgttgac cccgactttg gccaaaccat agaagttcga gtacagccac gccgccgtcg acgactggtt cacgattgcc ccgccaccgc gtttggccat gtgcttgtac acggcgcgcg tgcacaccag caccccgtcg tggttgacgc tcatgaactt cttgtagtaa tccaacggca ccgtcagcag taggtcgagc ttcatgccgc cgtagatcgc ggcgttgttc accaggtagt cgatgccgcc gaactcgctg accgtacgat cggccatcgc cctggccgac tcctcatcgg aaacatccac gggcacatga attgccgtgc caccgtcggc gacaatctgc tttgcggtct tctcggcggc ctcggcattg atgtcggcga cgaccacgcg cgcaccctcg cgagccaggg cgtccgcata cgcctggcca ataccctgcg ccgccccggt gacgatcgcc accttgttct cgaactgtcc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGQFENKV AIVTGAAQGI GQAYADALAR EGARVVVADI NAEAAEKTAK QIVADGGTAI HVPVDVSDEE SARAMADRTV SEFGGIDYLV NNAAIYGGMK LDLLLTVPLD YYKKFMSVNH DGVLVCTRAV YKHMAKRGGG AIVNQSSTAA WLYSNFYGLA KVGVNGLTQQ LSRELGGMKI RINAIAPGPI DTEATRTVTP GEFVKDMVKQ IPLSRMGTPE DLVGMCLFLL GDQASWITGQ IFNVDGGQII R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggacag ttcgagaaca aggtggcgat cgtcaccggg gcggcgcagg gtattggcca ggcgtatgcg gacgccctgg ctcgcgaggg tgcgcgcgtg gtcgtcgccg acatcaatgc cgaggccgcc gagaagaccg caaagcagat tgtcgccgac ggtggcacgg caattcatgt gcccgtggat gtttccgatg aggagtcggc cagggcgatg gccgatcgta cggtcagcga gttcggcggc atcgactacc tggtgaacaa cgccgcgatc tacggcggca tgaagctcga cctactgctg acggtgccgt tggattacta caagaagttc atgagcgtca accacgacgg ggtgctggtg tgcacgcgcg ccgtgtacaa gcacatggcc aaacgcggtg gcggggcaat cgtgaaccag tcgtcgacgg cggcgtggct gtactcgaac ttctatggtt tggccaaagt cggggtcaac gggcttaccc aacagctttc ccgtgagctt ggcggaatga agatccggat caatgcgatc gcgccgggac cgatcgacac cgaagccacc agaaccgtca cgcccggcga gttcgtcaag gacatggtca agcagatccc gttgagccgg atggggactc ccgaggacct ggtggggatg tgcctgttct tgctcggcga ccaggcctcc tggattaccg gacagatctt caatgtcgat ggcggccaga tcatccgcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MymaA.00414.b.A1.PS00821
PURIFICATION DATe: 10/19/2010
CONCENTRATION: data unavailable
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGQFENKV AIVTGAAQGI GQAYADALAR EGARVVVADI NAEAAEKTAK QIVADGGTAI HVPVDVSDEE SARAMADRTV SEFGGIDYLV NNAAIYGGMK LDLLLTVPLD YYKKFMSVNH DGVLVCTRAV YKHMAKRGGG AIVNQSSTAA WLYSNFYGLA KVGVNGLTQQ LSRELGGMKI RINAIAPGPI DTEATRTVTP GEFVKDMVKQ IPLSRMGTPE DLVGMCLFLL GDQASWITGQ IFNVDGGQII R
Validated NT Sequence
ttattagcgg atgatctggc cgccatcgac attgaagatc tgtccggtaa tccaggaggc ctggtcgccg agcaagaaca ggcacatccc caccaggtcc tcgggagtcc ccatccggct caacgggatc tgcttgacca tgtccttgac gaactcgccg ggcgtgacgg ttctggtggc ttcggtgtcg atcggtcccg gcgcgatcgc attgatccgg atcttcattc cgccaagctc acgggaaagc tgttgggtaa gcccgttgac cccgactttg gccaaaccat agaagttcga gtacagccac gccgccgtcg acgactggtt cacgattgcc ccgccaccgc gtttggccat gtgcttgtac acggcgcgcg tgcacaccag caccccgtcg tggttgacgc tcatgaactt cttgtagtaa tccaacggca ccgtcagcag taggtcgagc ttcatgccgc cgtagatcgc ggcgttgttc accaggtagt cgatgccgcc gaactcgctg accgtacgat cggccatcgc cctggccgac tcctcatcgg aaacatccac gggcacatga attgccgtgc caccgtcggc gacaatctgc tttgcggtct tctcggcggc ctcggcattg atgtcggcga cgaccacgcg cgcaccctcg cgagccaggg cgtccgcata cgcctggcca ataccctgcg ccgccccggt gacgatcgcc accttgttct cgaactgtcc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGQFENKV AIVTGAAQGI GQAYADALAR EGARVVVADI NAEAAEKTAK QIVADGGTAI HVPVDVSDEE SARAMADRTV SEFGGIDYLV NNAAIYGGMK LDLLLTVPLD YYKKFMSVNH DGVLVCTRAV YKHMAKRGGG AIVNQSSTAA WLYSNFYGLA KVGVNGLTQQ LSRELGGMKI RINAIAPGPI DTEATRTVTP GEFVKDMVKQ IPLSRMGTPE DLVGMCLFLL GDQASWITGQ IFNVDGGQII R
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGGACAG TTCGAGAACA AGGTGGCGAT CGTCACCGGG GCGGCGCAGG GTATTGGCCA GGCGTATGCG GACGCCCTGG CTCGCGAGGG TGCGCGCGTG GTCGTCGCCG ACATCAATGC CGAGGCCGCC GAGAAGACCG CAAAGCAGAT TGTCGCCGAC GGTGGCACGG CAATTCATGT GCCCGTGGAT GTTTCCGATG AGGAGTCGGC CAGGGCGATG GCCGATCGTA CGGTCAGCGA GTTCGGCGGC ATCGACTACC TGGTGAACAA CGCCGCGATC TACGGCGGCA TGAAGCTCGA CCTACTGCTG ACGGTGCCGT TGGATTACTA CAAGAAGTTC ATGAGCGTCA ACCACGACGG GGTGCTGGTG TGCACGCGCG CCGTGTACAA GCACATGGCC AAACGCGGTG GCGGGGCAAT CGTGAACCAG TCGTCGACGG CGGCGTGGCT GTACTCGAAC TTCTATGGTT TGGCCAAAGT CGGGGTCAAC GGGCTTACCC AACAGCTTTC CCGTGAGCTT GGCGGAATGA AGATCCGGAT CAATGCGATC GCGCCGGGAC CGATCGACAC CGAAGCCACC AGAACCGTCA CGCCCGGCGA GTTCGTCAAG GACATGGTCA AGCAGATCCC GTTGAGCCGG ATGGGGACTC CCGAGGACCT GGTGGGGATG TGCCTGTTCT TGCTCGGCGA CCAGGCCTCC TGGATTACCG GACAGATCTT CAATGTCGAT GGCGGCCAGA TCATCCGCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA