MypaA.01097.i
Rv0851c ortholog short chain dehydrogenase
| CENTER ID: | MypaA.01097.i |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01097.i.A1.GE29545 | Full length( MypaA.01097.i ) | 1 | 280 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01097.i.A1.PW29443 | Full length( MypaA.01097.i ) | 1 | 280 |
Structures
3TJR
DEPOSITED: 8/24/2011
DETERMINATION: XRay
CLONE: MypaA.01097.i.A1.GE29545
PROTEIN: MypaA.01097.i.A1.PW29443
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|262316.17.peg.749 |
| RefSeq: | NP_959644.1 |
| UniProt: | Q742X5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGFLSGFDG RAAVVTGGAS GIGLATATEF ARRGARLVLS DVDQPALEQA VNGLRGQGFD AHGVVCDVRH LDEMVRLADE AFRLLGGVDV VFSNAGIVVA GPLAQMNHDD WRWVIDIDLW GSIHAVEAFL PRLLEQGTGG HIAFTASFAG LVPNAGLGTY GVAKYGVVGL AETLAREVKP NGIGVSVLCP MVVETKLVSN SERIRGADYG MSATPEGAFG PLPTQDESVS ADDVARLTAD AILANRLYIL PHAAARESIR RRFERIDRTF DEQAAEGWTH
NT Sequence
ATGGACGGAT TCCTGTCTGG TTTCGACGGC CGCGCGGCGG TGGTCACCGG CGGCGCCAGC GGCATCGGAT TGGCCACCGC CACCGAATTC GCCCGCCGGG GGGCGCGTCT GGTGCTCTCC GACGTCGACC AGCCGGCGCT GGAACAGGCG GTCAACGGGC TGCGCGGCCA GGGGTTCGAC GCGCACGGCG TGGTGTGCGA TGTCCGGCAT CTCGACGAAA TGGTCCGTCT CGCCGATGAG GCCTTCCGGC TGCTGGGCGG CGTCGACGTC GTCTTCAGCA ACGCCGGCAT CGTCGTCGCG GGCCCGCTGG CCCAGATGAA CCATGACGAC TGGCGCTGGG TGATCGACAT CGACCTGTGG GGCTCCATCC ATGCCGTCGA GGCGTTCCTG CCCAGGCTGC TCGAGCAGGG CACCGGCGGG CACATCGCGT TCACCGCTTC CTTCGCCGGT CTGGTGCCCA ACGCCGGCCT CGGCACCTAC GGCGTTGCCA AATACGGGGT CGTCGGGCTC GCCGAAACGT TGGCGCGCGA AGTCAAGCCC AACGGCATCG GCGTATCGGT GTTGTGCCCG ATGGTGGTGG AGACCAAGCT GGTCTCCAAC TCGGAACGGA TTCGCGGCGC CGACTATGGG ATGTCGGCGA CGCCGGAGGG GGCCTTCGGG CCGCTGCCCA CCCAGGACGA GAGCGTCAGC GCCGACGACG TCGCGCGGCT CACCGCCGAC GCGATCCTGG CCAATCGGCT CTACATCCTG CCGCACGCGG CCGCCCGGGA GTCGATCCGC CGCAGGTTCG AACGCATCGA CCGGACCTTC GACGAGCAGG CCGCCGAGGG CTGGACGCAC
Details for MypaA.01097.i.A1.GE29545
| HARVESTED ON: | 6/15/2010 |
| SEQUENCED ON: | 6/27/2010 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDGFLSGFD GRAAVVTGGA SGIGLATATE FARRGARLVL SDVDQPALEQ AVNGLRGQGF DAHGVVCDVR HLDEMVRLAD EAFRLLGGVD VVFSNAGIVV AGPLAQMNHD DWRWVIDIDL WGSIHAVEAF LPRLLEQGTG GHIAFTASFA GLVPNAGLGT YGVAKYGVVG LAETLAREVK PNGIGVSVLC PMVVETKLVS NSERIRGADY GMSATPEGAF GPLPTQDESV SADDVARLTA DAILANRLYI LPHAAARESI RRRFERIDRT FDEQAAEGWT H
Validated NT Sequence
ttattagtgc gtccagccct cggcggcctg ctcgtcgaag gtccggtcga tgcgttcgaa cctgcggcgg atcgactccc gggcggccgc gtgcggcagg atgtagagcc gattggccag gatcgcgtcg gcggtgagcc gcgcgacgtc gtcggcgctg acgctctcgt cctgggtggg cagcggcccg aaggccccct ccggcgtcgc cgacatccca tagtcggcgc cgcgaatccg ttccgagttg gagaccagct tggtctccac caccatcggg cacaacaccg atacgccgat gccgttgggc ttgacttcgc gcgccaacgt ttcggcgagc ccgacgaccc cgtatttggc aacgccgtag gtgccgaggc cggcgttggg caccagaccg gcgaaggaag cggtgaacgc gatgtgcccg ccggtgccct gctcgagcag cctgggcagg aacgcctcga cggcatggat ggagccccac aggtcgatgt cgatcaccca gcgccagtcg tcatggttca tctgggccag cgggcccgcg acgacgatgc cggcgttgct gaagacgacg tcgacgccgc ccagcagccg gaaggcctca tcggcgagac ggaccatttc gtcgagatgc cggacatcgc acaccacgcc gtgcgcgtcg aacccctggc cgcgcagccc gttgaccgcc tgttccagcg ccggctggtc gacgtcggag agcaccagac gcgccccccg gcgggcgaat tcggtggcgg tggccaatcc gatgccgctg gcgccgccgg tgaccaccgc cgcgcggccg tcgaaaccag acaggaatcc gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDGFLSGFD GRAAVVTGGA SGIGLATATE FARRGARLVL SDVDQPALEQ AVNGLRGQGF DAHGVVCDVR HLDEMVRLAD EAFRLLGGVD VVFSNAGIVV AGPLAQMNHD DWRWVIDIDL WGSIHAVEAF LPRLLEQGTG GHIAFTASFA GLVPNAGLGT YGVAKYGVVG LAETLAREVK PNGIGVSVLC PMVVETKLVS NSERIRGADY GMSATPEGAF GPLPTQDESV SADDVARLTA DAILANRLYI LPHAAARESI RRRFERIDRT FDEQAAEGWT H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacggattc ctgtctggtt tcgacggccg cgcggcggtg gtcaccggcg gcgccagcgg catcggattg gccaccgcca ccgaattcgc ccgccggggg gcgcgtctgg tgctctccga cgtcgaccag ccggcgctgg aacaggcggt caacgggctg cgcggccagg ggttcgacgc gcacggcgtg gtgtgcgatg tccggcatct cgacgaaatg gtccgtctcg ccgatgaggc cttccggctg ctgggcggcg tcgacgtcgt cttcagcaac gccggcatcg tcgtcgcggg cccgctggcc cagatgaacc atgacgactg gcgctgggtg atcgacatcg acctgtgggg ctccatccat gccgtcgagg cgttcctgcc caggctgctc gagcagggca ccggcgggca catcgcgttc accgcttcct tcgccggtct ggtgcccaac gccggcctcg gcacctacgg cgttgccaaa tacggggtcg tcgggctcgc cgaaacgttg gcgcgcgaag tcaagcccaa cggcatcggc gtatcggtgt tgtgcccgat ggtggtggag accaagctgg tctccaactc ggaacggatt cgcggcgccg actatgggat gtcggcgacg ccggaggggg ccttcgggcc gctgcccacc caggacgaga gcgtcagcgc cgacgacgtc gcgcggctca ccgccgacgc gatcctggcc aatcggctct acatcctgcc gcacgcggcc gcccgggagt cgatccgccg caggttcgaa cgcatcgacc ggaccttcga cgagcaggcc gccgagggct ggacgcacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MypaA.01097.i.A1.PW29443
| PURIFICATION DATe: | 8/16/2010 |
| CONCENTRATION: | 32.82mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDGFLSGFD GRAAVVTGGA SGIGLATATE FARRGARLVL SDVDQPALEQ AVNGLRGQGF DAHGVVCDVR HLDEMVRLAD EAFRLLGGVD VVFSNAGIVV AGPLAQMNHD DWRWVIDIDL WGSIHAVEAF LPRLLEQGTG GHIAFTASFA GLVPNAGLGT YGVAKYGVVG LAETLAREVK PNGIGVSVLC PMVVETKLVS NSERIRGADY GMSATPEGAF GPLPTQDESV SADDVARLTA DAILANRLYI LPHAAARESI RRRFERIDRT FDEQAAEGWT H
Validated NT Sequence
ttattagtgc gtccagccct cggcggcctg ctcgtcgaag gtccggtcga tgcgttcgaa cctgcggcgg atcgactccc gggcggccgc gtgcggcagg atgtagagcc gattggccag gatcgcgtcg gcggtgagcc gcgcgacgtc gtcggcgctg acgctctcgt cctgggtggg cagcggcccg aaggccccct ccggcgtcgc cgacatccca tagtcggcgc cgcgaatccg ttccgagttg gagaccagct tggtctccac caccatcggg cacaacaccg atacgccgat gccgttgggc ttgacttcgc gcgccaacgt ttcggcgagc ccgacgaccc cgtatttggc aacgccgtag gtgccgaggc cggcgttggg caccagaccg gcgaaggaag cggtgaacgc gatgtgcccg ccggtgccct gctcgagcag cctgggcagg aacgcctcga cggcatggat ggagccccac aggtcgatgt cgatcaccca gcgccagtcg tcatggttca tctgggccag cgggcccgcg acgacgatgc cggcgttgct gaagacgacg tcgacgccgc ccagcagccg gaaggcctca tcggcgagac ggaccatttc gtcgagatgc cggacatcgc acaccacgcc gtgcgcgtcg aacccctggc cgcgcagccc gttgaccgcc tgttccagcg ccggctggtc gacgtcggag agcaccagac gcgccccccg gcgggcgaat tcggtggcgg tggccaatcc gatgccgctg gcgccgccgg tgaccaccgc cgcgcggccg tcgaaaccag acaggaatcc gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDGFLSGFD GRAAVVTGGA SGIGLATATE FARRGARLVL SDVDQPALEQ AVNGLRGQGF DAHGVVCDVR HLDEMVRLAD EAFRLLGGVD VVFSNAGIVV AGPLAQMNHD DWRWVIDIDL WGSIHAVEAF LPRLLEQGTG GHIAFTASFA GLVPNAGLGT YGVAKYGVVG LAETLAREVK PNGIGVSVLC PMVVETKLVS NSERIRGADY GMSATPEGAF GPLPTQDESV SADDVARLTA DAILANRLYI LPHAAARESI RRRFERIDRT FDEQAAEGWT H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGGATTC CTGTCTGGTT TCGACGGCCG CGCGGCGGTG GTCACCGGCG GCGCCAGCGG CATCGGATTG GCCACCGCCA CCGAATTCGC CCGCCGGGGG GCGCGTCTGG TGCTCTCCGA CGTCGACCAG CCGGCGCTGG AACAGGCGGT CAACGGGCTG CGCGGCCAGG GGTTCGACGC GCACGGCGTG GTGTGCGATG TCCGGCATCT CGACGAAATG GTCCGTCTCG CCGATGAGGC CTTCCGGCTG CTGGGCGGCG TCGACGTCGT CTTCAGCAAC GCCGGCATCG TCGTCGCGGG CCCGCTGGCC CAGATGAACC ATGACGACTG GCGCTGGGTG ATCGACATCG ACCTGTGGGG CTCCATCCAT GCCGTCGAGG CGTTCCTGCC CAGGCTGCTC GAGCAGGGCA CCGGCGGGCA CATCGCGTTC ACCGCTTCCT TCGCCGGTCT GGTGCCCAAC GCCGGCCTCG GCACCTACGG CGTTGCCAAA TACGGGGTCG TCGGGCTCGC CGAAACGTTG GCGCGCGAAG TCAAGCCCAA CGGCATCGGC GTATCGGTGT TGTGCCCGAT GGTGGTGGAG ACCAAGCTGG TCTCCAACTC GGAACGGATT CGCGGCGCCG ACTATGGGAT GTCGGCGACG CCGGAGGGGG CCTTCGGGCC GCTGCCCACC CAGGACGAGA GCGTCAGCGC CGACGACGTC GCGCGGCTCA CCGCCGACGC GATCCTGGCC AATCGGCTCT ACATCCTGCC GCACGCGGCC GCCCGGGAGT CGATCCGCCG CAGGTTCGAA CGCATCGACC GGACCTTCGA CGAGCAGGCC GCCGAGGGCT GGACGCACAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA