SSGCID: Target Page

MyavA.01097.h

Oxidoreductase, short chain dehydrogenase/reductase family protein

CENTER ID:	MyavA.01097.h
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01097.h.A1.GE29055	Full length( MyavA.01097.h )		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.01097.h.A1.PW28960	Full length( MyavA.01097.h )		1	312

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.3464
RefSeq:	YP_882721.1
UniProt:	A0A0H3A1N2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDLQGKVAV ITGGAGGIGR ALGRRLGHEG MKVVLADVLA DPLQEATRTL TDEGIEAAGV VTDVTDYSSV EALAKEALRR FGAVDVVCNN AGTGAVSEGY LWEHDLADWR WGIDVNVLGV IHGLKAFVPI LLERGEGHVV NTCSGNGGFA PIARGAMGGP ATAVYPMTKA AVLCVTESLY THLEMTGTRV RAHVLFPGGF LNTGIWESWR HRPPRYAPTQ QRRTPEQTLD KVVARFEAAG ARVEFTPLET VADLVVDGIA ADRFWMMGPP APSDDVVRRK AASILSRGAP DYLVDVLGRS AGGNSETQGE NQ

NT Sequence

ATGACGGACC TGCAGGGCAA GGTGGCGGTG ATCACCGGCG GCGCCGGAGG CATCGGCCGG GCCCTCGGCC GTCGTCTCGG CCACGAGGGC ATGAAGGTGG TGCTGGCCGA CGTGCTCGCC GACCCGCTGC AGGAGGCGAC GCGGACGCTG ACCGACGAGG GCATCGAGGC GGCCGGCGTG GTCACCGACG TCACCGACTA CTCCTCGGTT GAGGCGCTGG CCAAGGAGGC GTTGCGCCGC TTCGGCGCGG TGGACGTGGT GTGCAACAAC GCCGGTACCG GCGCGGTGTC CGAGGGCTAT CTGTGGGAGC ACGACCTGGC CGACTGGCGC TGGGGGATCG ACGTCAACGT GCTGGGCGTC ATCCACGGCC TCAAGGCGTT CGTGCCGATC CTGCTCGAGC GGGGCGAGGG GCACGTGGTC AACACCTGCT CGGGCAACGG CGGATTCGCG CCGATCGCGC GGGGCGCCAT GGGCGGACCG GCCACCGCGG TCTACCCGAT GACCAAGGCG GCCGTGCTGT GCGTGACCGA GAGCCTGTAC ACGCACCTGG AGATGACCGG GACGCGGGTG CGTGCGCACG TGCTGTTCCC GGGCGGGTTT TTGAACACCG GCATCTGGGA GTCGTGGCGG CATCGACCGC CGCGCTACGC CCCGACGCAG CAACGCCGCA CGCCCGAGCA GACGCTGGAC AAGGTGGTGG CCCGGTTCGA GGCCGCCGGC GCCCGCGTCG AGTTCACCCC GCTGGAGACG GTGGCCGACC TGGTGGTGGA CGGCATTGCG GCGGACCGGT TCTGGATGAT GGGCCCGCCC GCGCCGTCCG ACGACGTCGT GCGCCGCAAG GCGGCATCGA TCCTTTCCCG CGGCGCCCCG GACTACCTGG TCGACGTTCT CGGCCGCAGC GCCGGCGGAA ACTCCGAAAC GCAAGGAGAA AACCAG

Details for MyavA.01097.h.A1.GE29055

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MTDLQGKVAV ITGGAGGIGR ALGRRLGHEG MKVVLADVLA DPLREATRAL TDEGIEAAGV VTDVTDYSSV EALAKEALRR FGAVDVVCNN AGTGAVSEGY LWEHDLADWR WGIDVNVLGV IHGLKAFVPI LLERGEGHVV NTCSGNGGFA PIARGAMGGP ATAVYPMTKA AVLCLTESLY THLEMTGTRV RAHVLFPGGF LNTGIWESWR HRPPRYAPTQ QRRTPEQTLD KVVARFEAAG ARVEFTPLET VADLVVDGIA ADRFWMMGPP APSDDVVRRK AASILSRGAP DYLVDVLGRS AGGNSETQGE

Validated NT Sequence

tttctccttg cgtttcggag tttccgccgg cgctgcggcc gagaacgtcg accaggtagt ccggggcgcc gcgggaaagg atcgatgccg ccttgcggcg cacgacgtcg tcggacggcg cgggcgggcc catcatccag aaccggtccg ccgcaatgcc gtccaccacc aggtcggcca ccgtctccag cggggtgaac tcgacgcggg cgccggcggc ctcgaaccgg gccaccacct tgtccagcgt ctgctcgggc gtgcggcgtt gctgggtcgg ggcgtagcgc ggcggtcgat gccgccacga ctcccagatg ccggtgttca aaaacccgcc ggggaacagc acgtgcgccc gcacccgcgt cccggtcatc tcgaggtgcg tgtacaggct ctcggtcagg cacagcaccg cggccttggt catcgggtag accgcggtgg ccggtccgcc catggcgccc cgcgcgatcg gcgcgaatcc gccgttgccc gagcaggtgt tgaccacgtg cccctcgccc cgctcgagca ggatcggtac gaacgccttg aggccgtgga tgacgcccag cacgttgacg tcgatccccc agcgccagtc ggccaggtcg tgctcccaca gatagccctc ggacaccgcg ccggtaccgg cgttgttgca taccacgtcc accgcgccga agcggcgcaa cgcctccttg gccagcgcct cgaccgagga gtagtcggtg acgtcggtga ccacgccggc cgcctcgatg ccctcgtcgg tcagcgcccg cgtcgcctcc cgcagcgggt cggcgagcac gtcggccagc accaccttca tgccctcgtg gccgagacga cggccgaggg cccggccgat gcctccggcg ccgccggtga tcaccgccac cttgccctgc aggtccgtca tcgaaccagg accctgggtc tgagcttcca gggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTDLQGKVA VITGGAGGIG RALGRRLGHE GMKVVLADVL ADPLQEATRT LTDEGIEAAG VVTDVTDYSS VEALAKEALR RFGAVDVVCN NAGTGAVSEG YLWEHDLADW RWGIDVNVLG VIHGLKAFVP ILLERGEGHV VNTCSGNGGF APIARGAMGG PATAVYPMTK AAVLCVTESL YTHLEMTGTR VRAHVLFPGG FLNTGIWESW RHRPPRYAPT QQRRTPEQTL DKVVARFEAA GARVEFTPLE TVADLVVDGI AADRFWMMGP PAPSDDVVRR KAASILSRGA PDYLVDVLGR SAGGNSETQG ENQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggacctg cagggcaagg tggcggtgat caccggcggc gccggaggca tcggccgggc cctcggccgt cgtctcggcc acgagggcat gaaggtggtg ctggccgacg tgctcgccga cccgctgcag gaggcgacgc ggacgctgac cgacgagggc atcgaggcgg ccggcgtggt caccgacgtc accgactact cctcggttga ggcgctggcc aaggaggcgt tgcgccgctt cggcgcggtg gacgtggtgt gcaacaacgc cggtaccggc gcggtgtccg agggctatct gtgggagcac gacctggccg actggcgctg ggggatcgac gtcaacgtgc tgggcgtcat ccacggcctc aaggcgttcg tgccgatcct gctcgagcgg ggcgaggggc acgtggtcaa cacctgctcg ggcaacggcg gattcgcgcc gatcgcgcgg ggcgccatgg gcggaccggc caccgcggtc tacccgatga ccaaggcggc cgtgctgtgc gtgaccgaga gcctgtacac gcacctggag atgaccggga cgcgggtgcg tgcgcacgtg ctgttcccgg gcgggttttt gaacaccggc atctgggagt cgtggcggca tcgaccgccg cgctacgccc cgacgcagca acgccgcacg cccgagcaga cgctggacaa ggtggtggcc cggttcgagg ccgccggcgc ccgcgtcgag ttcaccccgc tggagacggt ggccgacctg gtggtggacg gcattgcggc ggaccggttc tggatgatgg gcccgcccgc gccgtccgac gacgtcgtgc gccgcaaggc ggcatcgatc ctttcccgcg gcgccccgga ctacctggtc gacgttctcg gccgcagcgc cggcggaaac tccgaaacgc aaggagaaaa ccagaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MyavA.01097.h.A1.PW28960

PURIFICATION DATe:	7/26/2010
CONCENTRATION:	24.68mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGACCTG CAGGGCAAGG TGGCGGTGAT CACCGGCGGC GCCGGAGGCA TCGGCCGGGC CCTCGGCCGT CGTCTCGGCC ACGAGGGCAT GAAGGTGGTG CTGGCCGACG TGCTCGCCGA CCCGCTGCAG GAGGCGACGC GGACGCTGAC CGACGAGGGC ATCGAGGCGG CCGGCGTGGT CACCGACGTC ACCGACTACT CCTCGGTTGA GGCGCTGGCC AAGGAGGCGT TGCGCCGCTT CGGCGCGGTG GACGTGGTGT GCAACAACGC CGGTACCGGC GCGGTGTCCG AGGGCTATCT GTGGGAGCAC GACCTGGCCG ACTGGCGCTG GGGGATCGAC GTCAACGTGC TGGGCGTCAT CCACGGCCTC AAGGCGTTCG TGCCGATCCT GCTCGAGCGG GGCGAGGGGC ACGTGGTCAA CACCTGCTCG GGCAACGGCG GATTCGCGCC GATCGCGCGG GGCGCCATGG GCGGACCGGC CACCGCGGTC TACCCGATGA CCAAGGCGGC CGTGCTGTGC GTGACCGAGA GCCTGTACAC GCACCTGGAG ATGACCGGGA CGCGGGTGCG TGCGCACGTG CTGTTCCCGG GCGGGTTTTT GAACACCGGC ATCTGGGAGT CGTGGCGGCA TCGACCGCCG CGCTACGCCC CGACGCAGCA ACGCCGCACG CCCGAGCAGA CGCTGGACAA GGTGGTGGCC CGGTTCGAGG CCGCCGGCGC CCGCGTCGAG TTCACCCCGC TGGAGACGGT GGCCGACCTG GTGGTGGACG GCATTGCGGC GGACCGGTTC TGGATGATGG GCCCGCCCGC GCCGTCCGAC GACGTCGTGC GCCGCAAGGC GGCATCGATC CTTTCCCGCG GCGCCCCGGA CTACCTGGTC GACGTTCTCG GCCGCAGCGC CGGCGGAAAC TCCGAAACGC AAGGAGAAAA CCAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA