MyabA.01097.k

Probable short-chain dehydrogenase/reductase

CENTER ID: MyabA.01097.k
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01097.k.A1.GE30370 Full length( MyabA.01097.k ) 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2108
RefSeq: YP_001702806.1
UniProt: B1MPA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLLPFGGAP GRTHRADAVV TGAGSGIGRA FAVELARRGG RVVCADRDPV TAKESAELVR QAGGEGFDIA CDVTDLEQIR NLADVSEDWF GKAASLVINN AGIGAGGNRI GATSIEDWKA AISVNLWGVI YGCETFVPRL RSSGHGGVIN VASAASFGSA PRMAAYNVSK AGVLALSETL AAELSGTDVK VTVLCPTFVK TNIVKNPQID EAAARLAANL MKWTGISPQH VARTTLNAHD RGQIYVVPQL DAKVLWQLKR ALPGPFTRTL GLVERMASWN DSAE
NT Sequence
ATGAGATTGC TGCCATTCGG CGGCGCACCC GGCAGAACGC ACCGAGCCGA TGCAGTCGTC ACGGGCGCAG GAAGCGGTAT CGGCCGCGCA TTCGCCGTGG AGCTTGCACG CCGAGGCGGA CGCGTGGTGT GCGCCGACAG AGATCCCGTC ACCGCAAAAG AGTCGGCAGA GTTGGTGAGG CAGGCTGGCG GCGAAGGCTT CGACATCGCA TGCGACGTCA CCGATCTTGA GCAGATCCGC AACCTCGCTG ATGTGAGCGA AGACTGGTTC GGGAAGGCCG CGAGCCTCGT CATCAATAAC GCCGGAATCG GTGCAGGTGG TAACCGAATC GGCGCGACGT CAATCGAGGA CTGGAAAGCG GCGATTTCGG TCAACCTCTG GGGCGTTATC TACGGCTGCG AGACTTTTGT GCCGCGGCTC CGCAGCAGTG GGCATGGCGG AGTCATCAAT GTGGCGTCCG CAGCGAGCTT TGGCTCGGCC CCTCGAATGG CGGCTTACAA CGTGAGCAAG GCCGGAGTCC TCGCTCTGTC GGAGACATTG GCGGCCGAAC TGAGCGGTAC TGACGTCAAG GTCACAGTGC TTTGCCCCAC CTTCGTGAAG ACGAACATCG TCAAAAATCC TCAAATCGAC GAGGCGGCTG CGAGGCTCGC GGCCAACCTG ATGAAATGGA CTGGCATTTC GCCACAGCAC GTTGCTCGAA CGACGTTGAA CGCGCATGAT CGCGGACAAA TTTATGTAGT GCCCCAACTC GATGCCAAAG TCTTGTGGCA ACTCAAGAGA GCCCTACCCG GTCCTTTTAC ACGCACGCTG GGCCTCGTGG AGCGCATGGC CTCATGGAAC GATTCAGCCG AG
Details for MyabA.01097.k.A1.GE30370
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLLPFGGA PGRTHRADAV VTGAGSGIGR AFAVELARRG GRVVCADRDP VTAKESAELV RQAGGEGFDI ACDVTDLEQI RNLADVSEDW FGKAASLVIN NAGIGAGGNR IGATSIEDWK AAISVNLWGV IYGCETFVPR LRSSGHGGVI NVASAASFGS APRMAAYNVS KAGVLALSET LAAELSGTDV KVTVLCPTFV KTNIVKNPQI DEAAARLAAX LMKWTGISPQ HVARTTLNAH DRGQIYVVPQ LDAXVLWQLK RALPGPFTRT LGLVXRXXLM ERFXRVXNST NKFCSXASRG SXC
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagatt gctgccattc ggcggcgcac ccggcagaac gcaccgagcc gatgcagtcg tcacgggcgc aggaagcggt atcggccgcg cattcgccgt ggagcttgca cgccgaggcg gacgcgtggt gtgcgccgac agagatcccg tcaccgcaaa agagtcggca gagttggtga ggcaggctgg cggcgaaggc ttcgacatcg catgcgacgt caccgatctt gagcagatcc gcaacctcgc tgatgtgagc gaagactggt tcgggaaggc cgcgagcctc gtcatcaata acgccggaat cggtgcaggt ggtaaccgaa tcggcgcgac gtcaatcgag gactggaaag cggcgatttc ggtcaacctc tggggcgtta tctacggctg cgagactttt gtgccgcggc tccgcagcag tgggcatggc ggagtcatca atgtggcgtc cgcagcgagc tttggctcgg cccctcgaat ggcggcttac aacgtgagca aggccggagt cctcgctctg tcggagacat tggcggccga actgagcggt actgacgtca aggtcacagt gctttgcccc accttcgtga agacgaacat cgtcaaaaat cctcaaatcg acgaggcggc tgcgaggctc gcggccnacc tgatgaaatg gactggcatt tcgccacagc acgttgctcg aacgacgttg aacgcgcatg atcgcggaca aatttatgta gtgccccaac tcgatgccna agtcttgtgg caactcaaga gagccctacc cggtcctttt acacgcacgc tgggcctcgt ggnncgcatn gncctcatgg aacgattcan ccgagtanta aacagcacga acaagttctg cagccnagct tcncgaggat ccngctgcta acaaagccng aaaggaagct gannnngctg ctgccnncnn ntg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLLPFGGA PGRTHRADAV VTGAGSGIGR AFAVELARRG GRVVCADRDP VTAKESAELV RQAGGEGFDI ACDVTDLEQI RNLADVSEDW FGKAASLVIN NAGIGAGGNR IGATSIEDWK AAISVNLWGV IYGCETFVPR LRSSGHGGVI NVASAASFGS APRMAAYNVS KAGVLALSET LAAELSGTDV KVTVLCPTFV KTNIVKNPQI DEAAARLAAN LMKWTGISPQ HVARTTLNAH DRGQIYVVPQ LDAKVLWQLK RALPGPFTRT LGLVERMASW NDSAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagattgctg ccattcggcg gcgcacccgg cagaacgcac cgagccgatg cagtcgtcac gggcgcagga agcggtatcg gccgcgcatt cgccgtggag cttgcacgcc gaggcggacg cgtggtgtgc gccgacagag atcccgtcac cgcaaaagag tcggcagagt tggtgaggca ggctggcggc gaaggcttcg acatcgcatg cgacgtcacc gatcttgagc agatccgcaa cctcgctgat gtgagcgaag actggttcgg gaaggccgcg agcctcgtca tcaataacgc cggaatcggt gcaggtggta accgaatcgg cgcgacgtca atcgaggact ggaaagcggc gatttcggtc aacctctggg gcgttatcta cggctgcgag acttttgtgc cgcggctccg cagcagtggg catggcggag tcatcaatgt ggcgtccgca gcgagctttg gctcggcccc tcgaatggcg gcttacaacg tgagcaaggc cggagtcctc gctctgtcgg agacattggc ggccgaactg agcggtactg acgtcaaggt cacagtgctt tgccccacct tcgtgaagac gaacatcgtc aaaaatcctc aaatcgacga ggcggctgcg aggctcgcgg ccaacctgat gaaatggact ggcatttcgc cacagcacgt tgctcgaacg acgttgaacg cgcatgatcg cggacaaatt tatgtagtgc cccaactcga tgccaaagtc ttgtggcaac tcaagagagc cctacccggt ccttttacac gcacgctggg cctcgtggag cgcatggcct catggaacga ttcagccgag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa