SSGCID: Target Page

MypaA.01097.a

Putative uncharacterized protein

CENTER ID:	MypaA.01097.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01097.a.A2.GE29537	Mature protein( MypaA.01097.a )		31	282

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.2337
RefSeq:	NP_961132.1
UniProt:	Q73XW0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDRLLHRSK TSRGALAVVT GAGSGIGAAF ALELGKRGGT VVCSDIDQAA AQRTADAITQ HGAKALATRC DVSQFGDVQA LAEQSQSCFG APPTLVINNA GVGAGGAAIG DAPLDDWQWT LGINLWGPIH GCHVFTPILR DAAPSAAPRG IINVASAAAF GAAPGMAAYN VSKAGVLSLS ETLAAELSGT PVRVTVLCPT FVKTNILESG RISEESGELA AKLMRWTGFS ADKVARICLD AHDRGDLYCM PQLDAQIGWH IKRLAPQAYT RAAGLVSRIN LP

NT Sequence

ATGTTGGACC GGCTGCTGCA CCGATCGAAG ACCAGCCGCG GCGCCCTGGC GGTGGTGACC GGCGCGGGAA GCGGCATCGG TGCGGCCTTC GCCCTCGAAC TGGGCAAGCG CGGCGGCACC GTCGTGTGCA GCGATATCGA CCAAGCCGCC GCCCAGCGGA CGGCCGACGC GATCACCCAA CACGGCGCGA AAGCCCTCGC AACCCGTTGT GATGTCTCGC AATTCGGCGA CGTGCAGGCG CTGGCCGAGC AGTCGCAGTC CTGCTTCGGC GCCCCACCCA CACTGGTGAT CAACAACGCC GGTGTCGGCG CCGGCGGCGC GGCCATCGGT GATGCGCCAC TGGACGATTG GCAGTGGACG CTCGGAATCA ACCTGTGGGG TCCCATTCAC GGCTGTCATG TGTTCACCCC GATCCTGCGC GACGCCGCGC CGTCGGCCGC GCCGCGGGGC ATCATCAACG TCGCCTCGGC CGCGGCGTTC GGCGCGGCCC CCGGCATGGC TGCCTACAAC GTCAGCAAGG CGGGCGTGCT GTCACTGTCC GAGACCCTGG CCGCCGAGCT GTCCGGCACG CCGGTGCGGG TCACCGTGCT GTGCCCGACG TTCGTCAAGA CGAACATCCT CGAATCCGGG CGAATCAGCG AGGAGTCCGG CGAACTGGCC GCAAAACTGA TGCGCTGGAC CGGGTTCTCC GCGGACAAAG TCGCCCGCAT CTGCTTGGAC GCGCACGACC GCGGCGACCT GTACTGCATG CCGCAGCTCG ACGCACAAAT CGGCTGGCAC ATCAAACGCC TTGCCCCGCA GGCCTATACG CGAGCAGCCG GCCTGGTGTC CCGAATCAAC CTGCCC

Details for MypaA.01097.a.A2.GE29537

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLXAQTQGPG SMALELGKRG GTVVCSDIDQ AAAQRTADAI TQHGAKALAT RCDVSQFGDV QALAEQSQSC FGAPPTLVIN NAGVGAGGAA IGDAPLDDWQ WTLGINLWGP IHGCHVFTPI LRDAAPSAAP RGIINVASAA AFGAAPGMAA YNVSKAGVLS LSETLAAELS GTPVRVTVLC PTFVKTNILE SGRISEESGE LAAKLMRWTG FSADKVARIC LDAHDRGDLY CMPQLDAQIG WHIKRLAPQA YTRAAGLVSR INLP

Validated NT Sequence

tttgttnnct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggnagct cagacccagg gtcctggttc gatggccctc gaactgggca agcgcggcgg caccgtcgtg tgcagcgata tcgaccaagc cgccgcccag cggacggccg acgcgatcac ccaacacggc gcgaaagccc tcgcaacccg ttgtgatgtc tcgcaattcg gcgacgtgca ggcgctggcc gagcagtcgc agtcctgctt cggcgcccca cccacactgg tgatcaacaa cgccggtgtc ggcgccggcg gcgcggccat cggtgatgcg ccactggacg attggcagtg gacgctcgga atcaacctgt ggggtcccat tcacggctgt catgtgttca ccccgatcct gcgcgacgcc gcgccgtcgg ccgcgccgcg gggcatcatc aacgtcgcct cggccgcggc gttcggcgcg gcccccggca tggctgccta caacgtcagc aaggcgggcg tgctgtcact gtccgagacc ctggccgccg agctgtccgg cacgccggtg cgggtcaccg tgctgtgccc gacgttcgtc aagacgaaca tcctcgaatc cgggcgaatc agcgaggagt ccggcgaact ggccgcaaaa ctgatgcgct ggaccgggtt ctccgcggac aaagtcgccc gcatctgctt ggacgcgcac gaccgcggcg acctgtactg catgccgcag ctcgacgcac aaatcggctg gcacatcaaa cgccttgccc cgcaggccta tacgcgagca gccggcctgg tgtcccgaat caacctgccc taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccnaaagg aanctgagtt ggctgctgcc accgntna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMALELGKRG GTVVCSDIDQ AAAQRTADAI TQHGAKALAT RCDVSQFGDV QALAEQSQSC FGAPPTLVIN NAGVGAGGAA IGDAPLDDWQ WTLGINLWGP IHGCHVFTPI LRDAAPSAAP RGIINVASAA AFGAAPGMAA YNVSKAGVLS LSETLAAELS GTPVRVTVLC PTFVKTNILE SGRISEESGE LAAKLMRWTG FSADKVARIC LDAHDRGDLY CMPQLDAQIG WHIKRLAPQA YTRAAGLVSR INLP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccctcgaa ctgggcaagc gcggcggcac cgtcgtgtgc agcgatatcg accaagccgc cgcccagcgg acggccgacg cgatcaccca acacggcgcg aaagccctcg caacccgttg tgatgtctcg caattcggcg acgtgcaggc gctggccgag cagtcgcagt cctgcttcgg cgccccaccc acactggtga tcaacaacgc cggtgtcggc gccggcggcg cggccatcgg tgatgcgcca ctggacgatt ggcagtggac gctcggaatc aacctgtggg gtcccattca cggctgtcat gtgttcaccc cgatcctgcg cgacgccgcg ccgtcggccg cgccgcgggg catcatcaac gtcgcctcgg ccgcggcgtt cggcgcggcc cccggcatgg ctgcctacaa cgtcagcaag gcgggcgtgc tgtcactgtc cgagaccctg gccgccgagc tgtccggcac gccggtgcgg gtcaccgtgc tgtgcccgac gttcgtcaag acgaacatcc tcgaatccgg gcgaatcagc gaggagtccg gcgaactggc cgcaaaactg atgcgctgga ccgggttctc cgcggacaaa gtcgcccgca tctgcttgga cgcgcacgac cgcggcgacc tgtactgcat gccgcagctc gacgcacaaa tcggctggca catcaaacgc cttgccccgc aggcctatac gcgagcagcc ggcctggtgt cccgaatcaa cctgcccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa