SSGCID: Target Page

MymaA.01097.d

Short-chain membrane-associated dehydrogenase

CENTER ID:	MymaA.01097.d
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01097.d.A1.GE30138	Full length( MymaA.01097.d )		1	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3358
RefSeq:	YP_001851430.1
UniProt:	B2HG11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFLPFGSSP GRTHRAHAVV TGAGSGIGRA FAVELARRGG RVVCADKDPI TAKESAELVR QAGGEGFDVV CDVTDLEQVR NLADASEDWF GKAASLVINN AGIGAGGNRI GATSVEDWNA AISVNLWGVI YGCETFVPRL RSNGRGGVIN VASAASFGSA PRMGAYNVSK AGVLALSETL AAELSGTNVN VTVLCPTFVK TNIAKNPQIE ESAAKLATNL MRWTGISPDQ VARTTLNAHD RGQIYVVPQL DAKILWQLKR ALPGQFTRAL GLVERVASWN DPAEQREQ

NT Sequence

ATGAGGTTCT TGCCGTTCGG TAGTTCACCA GGTAGAACCC ACCGCGCCCA CGCAGTAGTC ACAGGCGCAG GGAGCGGTAT CGGCCGCGCG TTCGCCGTTG AGCTTGCACG CCGAGGTGGA CGTGTTGTGT GTGCGGACAA AGACCCCATC ACTGCGAAAG AGTCGGCCGA GTTGGTGAGG CAGGCCGGCG GCGAGGGTTT CGACGTCGTA TGCGACGTCA CCGACCTTGA GCAGGTCCGT AACCTCGCTG ATGCCAGTGA AGACTGGTTT GGCAAGGCGG CGAGCCTGGT GATCAACAAC GCCGGAATCG GTGCGGGCGG CAATCGCATC GGCGCTACGT CGGTCGAGGA CTGGAACGCT GCGATTTCTG TCAACCTTTG GGGTGTTATC TACGGGTGCG AGACTTTCGT TCCCCGGCTC CGGAGCAACG GCCGTGGCGG AGTGATCAAT GTGGCGTCCG CCGCGAGTTT CGGCTCGGCG CCCCGAATGG GGGCATACAA CGTGAGTAAG GCCGGAGTGC TCGCTCTGTC CGAGACCTTG GCGGCCGAAC TGAGCGGTAC TAACGTCAAT GTCACAGTGC TTTGCCCCAC CTTCGTCAAG ACGAACATCG CCAAAAATCC TCAGATTGAG GAGTCGGCGG CGAAACTCGC GACCAACCTG ATGAGGTGGA CCGGCATTTC GCCGGATCAA GTGGCTCGAA CGACCTTGAA CGCGCACGAT CGCGGACAAA TCTATGTAGT GCCCCAACTG GACGCGAAAA TTTTGTGGCA ACTGAAAAGA GCTTTACCCG GTCAGTTTAC GCGGGCGTTG GGGCTGGTGG AGCGCGTGGC CTCATGGAAC GATCCAGCCG AACAGAGGGA GCAG

Details for MymaA.01097.d.A1.GE30138

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRFLPFGSS PGRTHRAHAV VTGAGSGIGR AFAVELARRG GRVVCADKDP ITAKESAELV RQAGGEGFDV VCDVTDLEQV RNLADASEDW FGKAASLVIN NAGIGAGGNR IGATSVEDWN AAISVNLWGV IYGCETFVPR LRSNGRGGVI NVASAASFGS APRMGAYNVS KAGVLALSET LAAELSGTNV NVTVLCPTFV KTNIAKNPQI EESAAKLATN LMRWTGISPD QVARTTLNAH DRGQIYVVPQ LDAKILWQLK RALPGQFTRA LGLVERVASW NDPAEQR

Validated NT Sequence

ccctctgttc ggctggatcg ttccatgagg ccacgcgctc caccagcccc aacgcccgcg taaactgacc gggtaaagct cttttcagtt gccacaaaat tttcgcgtcc agttggggca ctacatagat ttgtccgcga tcgtgcgcgt tcaaggtcgt tcgagccact tgatccggcg aaatgccggt ccacctcatc aggttggtcg cgagtttcgc cgccgactcc tcaatctgag gatttttggc gatgttcgtc ttgacgaagg tggggcaaag cactgtgaca ttgacgttag taccgctcag ttcggccgcc aaggtctcgg acagagcgag cactccggcc ttactcacgt tgtatgcccc cattcggggc gccgagccga aactcgcggc ggacgccaca ttgatcactc cgccacggcc gttgctccgg agccggggaa cgaaagtctc gcacccgtag ataacacccc aaaggttgac agaaatcgca gcgttccagt cctcgaccga cgtagcgccg atgcgattgc cgcccgcacc gattccggcg ttgttgatca ccaggctcgc cgccttgcca aaccagtctt cactggcatc agcgaggtta cggacctgct caaggtcggt gacgtcgcat acgacgtcga aaccctcgcc gccggcctgc ctcaccaact cggccgactc tttcgcagtg atggggtctt tgtccgcaca cacaacacgt ccacctcggc gtgcaagctc aacggcgaac gcgcggccga taccgctccc tgcgcctgtg actactgcgt gggcgcggtg ggttctacct ggtgaactac cgaacggcaa gaacctcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaggttcttg ccgttcggta gttcaccagg tagaacccac cgcgcccacg cagtagtcac aggcgcaggg agcggtatcg gccgcgcgtt cgccgttgag cttgcacgcc gaggtggacg tgttgtgtgt gcggacaaag accccatcac tgcgaaagag tcggccgagt tggtgaggca ggccggcggc gagggtttcg acgtcgtatg cgacgtcacc gaccttgagc aggtccgtaa cctcgctgat gccagtgaag actggtttgg caaggcggcg agcctggtga tcaacaacgc cggaatcggt gcgggcggca atcgcatcgg cgctacgtcg gtcgaggact ggaacgctgc gatttctgtc aacctttggg gtgttatcta cgggtgcgag actttcgttc cccggctccg gagcaacggc cgtggcggag tgatcaatgt ggcgtccgcc gcgagtttcg gctcggcgcc ccgaatgggg gcatacaacg tgagtaaggc cggagtgctc gctctgtccg agaccttggc ggccgaactg agcggtacta acgtcaatgt cacagtgctt tgccccacct tcgtcaagac gaacatcgcc aaaaatcctc agattgagga gtcggcggcg aaactcgcga ccaacctgat gaggtggacc ggcatttcgc cggatcaagt ggctcgaacg accttgaacg cgcacgatcg cggacaaatc tatgtagtgc cccaactgga cgcgaaaatt ttgtggcaac tgaaaagagc tttacccggt cagtttacgc gggcgttggg gctggtggag cgcgtggcct catggaacga tccagccgaa cagagggagc agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa