MyulA.18627.b

Linoleoyl-CoA desaturase, DesA3_1 (Rv3229c ortholog)

CENTER ID: MyulA.18627.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.18627.b.B1.GE37682 full length 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3007
RefSeq: YP_906377.1
UniProt: A0PRD7

Orthologues for Rv3229c

Species % Identity % Coverage Clones Proteins Structures
marinum 88 100 0 0 0
ulcerans 88 100 1 0 0
paratuberculosis 85 100 1 0 0
marinum 81 100 0 0 0
ulcerans 80 100 1 0 0
thermoresistibile 80 98 0 0 0
fortuitum 79 96 1 0 0
abscessus 74 97 0 0 0
abscessus 69 97 1 0 0
paratuberculosis 62 20 0 0 0
paratuberculosis 60 30 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAITDVDVFA HLTDGDIENL STELDDIRRD IEESRGERDA RYIYRTIAAQ RALEVSGRLV LAASSRRSAW WVGAVTLSVA KIIENMEIGH NVMHGQWDWM NDPEVHSSTW EWDMASTSKH WRYTHNVVHH KYTNILDMDH DVGYGMLRVT RDQGWRRRDA FNLAINALLA IGFEWGIALR HLEARDLFKD GPEREAAKFR LREFSGKAGR QAVKDYVAFP ALTSVSPAAT YRSTLTANAF ANVIRNVWSN AVIFCGHFPD GAEKFTKTEL VDETQGQWYL RQMLGSANFN AGAAMRFMSG SLSHQIEHHL YPDLPSNRLA EVSVRVREIC DKYDLPYTTG SFPLQYAKTW RTLAKLSLPN KYLRDNADDA PETRSERMFA GLEAGFAATD PMTGRRRGLK AAITAVRAWR RAKPGKAKKA GGTDGLAA
NT Sequence
gtggcgataa ccgatgtcga cgtgttcgcg catctgacgg acggcgatat cgaaaacctg agcactgaac tcgatgacat ccgccgcgac attgaagaat cgcgcggtga acgcgatgcc cgctacatct accgaaccat cgccgcacag cgggcgctgg aggtcagtgg ccggttggtg ctggcggcca gctcgcgtcg ctcggcgtgg tgggtaggtg cggtgacgct cagcgtggcc aagatcatcg agaacatgga gatcggtcac aacgtcatgc atggccaatg ggattggatg aatgatcccg aggtgcactc ctcgacgtgg gagtgggaca tggcctcgac gtcgaagcac tggcggtata cccacaacgt ggtgcaccac aagtacacca acattctcga catggatcac gatgtcggct acggcatgct gcgggtgacc cgggatcaag ggtggcggcg gcgagacgcc ttcaacctgg ccatcaatgc tctgcttgcg atcgggttcg aatggggaat cgcgttgcgg cacttggagg ctcgcgacct cttcaaagat gggccagagc gcgaagctgc caaattcagg ctacgggagt tctcgggtaa ggccggtcgt caggcggtca aagactatgt ggccttcccg gcgttgacgt cggtgtcgcc tgccgcgacc taccggtcta ccttgaccgc caacgcgttt gccaatgtga tccgcaacgt ctggtccaac gccgttattt tctgtgggca tttcccggac ggcgcagaga aattcaccaa gactgagttg gtagacgaga cccagggcca gtggtacctg cggcagatgt tgggaagcgc caatttcaat gctggagcgg cgatgcgctt catgagcggc agcctcagtc atcaaatcga gcaccatctc tatccggact tgccgagcaa tcggctggcc gaggtttcgg tgcgggtgcg cgagatctgc gataagtacg acttgcctta caccacaggc tcattcccgc tgcagtacgc caagacatgg cgcacgctcg ccaagctctc gctgccgaac aagtacctgc gcgacaacgc cgacgacgct ccggagaccc gcagcgagcg catgttcgcc ggtctggagg cgggcttcgc ggcaaccgat ccgatgaccg gccgtcgtcg cggcctcaag gccgcgataa ccgcggtacg cgcctggcgg cgcgccaagc ccggcaaggc caagaaggcc ggcggcaccg acggtctggc ggcctag
Details for MyulA.18627.b.B1.GE37682
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMV AITDVDVFAH LTDGDIENLS TELDDIRRDI EESRGERDAR YIYRTIAAQR ALEVSGRLVL AASSRRSAWW VGAVTLSVAK IIENMEIGHN VMHGQWDWMN DPEVHSSTWE WDMASTSKHW RYTHNVVHHK YTNILDMDHD VGYGMLRVTR DQGWRRRDAF NLAINALLAI GFEWGIALRH LEARDLFKDG PEREAAKFRL REFSGKAGRQ AVKDYVAFPA LTSVSPAATY RSTLTANAFA NVIRNVWSNA VIFCGHFPDG AEKFTKTELV DETQGQWYLR QMLGSANFNA GAAMRFMSGS LSHQIEHHLY PDLPSNRLAE VSVRVXEICD KYDXXTXXSX RCSTPDMXXL ASSRCXXVLX XXXXXSXEPX RXXXXXGXXX XXXXXXPWXX GXXXPXXXX
Validated NT Sequence
cnctaganta attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatggt ggcgataacc gatgtcgacg tgttcgcgca tctgacggac ggcgatatcg aaaacctgag cactgaactc gatgacatcc gccgcgacat tgaagaatcg cgcggtgaac gcgatgcccg ctacatctac cgaaccatcg ccgcacagcg ggcgctggag gtcagtggcc ggttggtgct ggcggccagc tcgcgtcgct cggcgtggtg ggtaggtgcg gtgacgctca gcgtggccaa gatcatcgag aacatggaga tcggtcacaa cgtcatgcat ggccaatggg attggatgaa tgatcccgag gtgcactcct cgacgtggga gtgggacatg gcctcgacgt cgaagcactg gcggtatacc cacaacgtgg tgcaccacaa gtacaccaac attctcgaca tggatcacga tgtcggctac ggcatgctgc gggtgacccg ggatcaaggg tggcggcggc gagacgcctt caacctggcc atcaatgctc tgcttgcgat cgggttcgaa tggggaatcg cgttgcggca cttggaggct cgcgacctct tcaaagatgg gccagagcgc gaagctgcca aattcaggct acgggagttc tcgggtaagg ccggtcgtca ggcggtcaaa gactatgtgg ccttcccggc gttgacgtcg gtgtcgcctg ccgcgaccta ccggtctacc ttgaccgcca acgcgtttgc caatgtgatc cgcaacgtct ggtccaacgc cgttattttc tgtgggcatt tcccggacgg cgcagagaaa ttcaccaaga ctgagttggt agacgagacc cagggccagt ggtacctgcg gcagatgttg ggaagcgcca atttcaatgc tggagcggcg atgcgcttca tgagcggcag cctcagtcat caaatcgagc accatctcta tccggacttg ccgagcaatc ggctggccga ggtttcggtg cgggtgcncg agatctgcga taagtacgac ttncntacnc ncagntcatn ccgctgcagt acgccagaca tgnnnnngct cgcnagctct cgctgcnaac nagtactgnn cgannnnnna cnannctccg nagaaccnca ncgannnann nnnnnnnntg gnagnngntt nngnnncnat cnnnnnncgc cntggnnnna nggccnnnnn nacccnnnnn nancnnccnt
Expected Protein Sequence
MAHHHHHHMA ITDVDVFAHL TDGDIENLST ELDDIRRDIE ESRGERDARY IYRTIAAQRA LEVSGRLVLA ASSRRSAWWV GAVTLSVAKI IENMEIGHNV MHGQWDWMND PEVHSSTWEW DMASTSKHWR YTHNVVHHKY TNILDMDHDV GYGMLRVTRD QGWRRRDAFN LAINALLAIG FEWGIALRHL EARDLFKDGP EREAAKFRLR EFSGKAGRQA VKDYVAFPAL TSVSPAATYR STLTANAFAN VIRNVWSNAV IFCGHFPDGA EKFTKTELVD ETQGQWYLRQ MLGSANFNAG AAMRFMSGSL SHQIEHHLYP DLPSNRLAEV SVRVREICDK YDLPYTTGSF PLQYAKTWRT LAKLSLPNKY LRDNADDAPE TRSERMFAGL EAGFAATDPM TGRRRGLKAA ITAVRAWRRA KPGKAKKAGG TDGLAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcga taaccgatgt cgacgtgttc gcgcatctga cggacggcga tatcgaaaac ctgagcactg aactcgatga catccgccgc gacattgaag aatcgcgcgg tgaacgcgat gcccgctaca tctaccgaac catcgccgca cagcgggcgc tggaggtcag tggccggttg gtgctggcgg ccagctcgcg tcgctcggcg tggtgggtag gtgcggtgac gctcagcgtg gccaagatca tcgagaacat ggagatcggt cacaacgtca tgcatggcca atgggattgg atgaatgatc ccgaggtgca ctcctcgacg tgggagtggg acatggcctc gacgtcgaag cactggcggt atacccacaa cgtggtgcac cacaagtaca ccaacattct cgacatggat cacgatgtcg gctacggcat gctgcgggtg acccgggatc aagggtggcg gcggcgagac gccttcaacc tggccatcaa tgctctgctt gcgatcgggt tcgaatgggg aatcgcgttg cggcacttgg aggctcgcga cctcttcaaa gatgggccag agcgcgaagc tgccaaattc aggctacggg agttctcggg taaggccggt cgtcaggcgg tcaaagacta tgtggccttc ccggcgttga cgtcggtgtc gcctgccgcg acctaccggt ctaccttgac cgccaacgcg tttgccaatg tgatccgcaa cgtctggtcc aacgccgtta ttttctgtgg gcatttcccg gacggcgcag agaaattcac caagactgag ttggtagacg agacccaggg ccagtggtac ctgcggcaga tgttgggaag cgccaatttc aatgctggag cggcgatgcg cttcatgagc ggcagcctca gtcatcaaat cgagcaccat ctctatccgg acttgccgag caatcggctg gccgaggttt cggtgcgggt gcgcgagatc tgcgataagt acgacttgcc ttacaccaca ggctcattcc cgctgcagta cgccaagaca tggcgcacgc tcgccaagct ctcgctgccg aacaagtacc tgcgcgacaa cgccgacgac gctccggaga cccgcagcga gcgcatgttc gccggtctgg aggcgggctt cgcggcaacc gatccgatga ccggccgtcg tcgcggcctc aaggccgcga taaccgcggt acgcgcctgg cggcgcgcca agcccggcaa ggccaagaag gccggcggca ccgacggtct ggcggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at