MyabA.18634.a

Putative fatty acid desaturase (Rv3229c ortholog)

CENTER ID: MyabA.18634.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.18634.a.B1.GE37335 full length 1 407
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3618
RefSeq: YP_001704278.1
UniProt: B1MF18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAISDIQEFA HLTEADVEAL GHELDAIRRD VEESLGAADA RYIRRTIAAQ RTLELTGRIL LAASKKRSAW WAGAVTLGVA KIVENMEIGH NVMHGQWDWM NDPEIHSSSW EWDMVDPAEH WKQTHNYIHH KYTNVLGMDD DVGYGLLRVT RDKKWKPFNL GNLLYNTLLM LLFEWGVGTQ HLELGKVAQG RADKEEFRVK RQAALTKMGK QVAKDYVAFP ALSALSPAGS YRKTVTANLL ANVIRNIWSN AVIFCGHFPD GAEKFTKQDL ENETQGQWYL RQMLGSANFD AGPAMAFMSG NLCHQIEHHL FPDLPSNRLS AVSVRVRELC DKYDLPYTSG PFLVQYAKAW RTIAKLSLPD KYLTYTADNA QETRSVKMFD ELEPHERRGL KSAFAAVRAR RKARHKV
NT Sequence
atggcgattt cggatatcca ggaattcgcg cacctcaccg aggctgatgt tgaggcgctc gggcacgagt tggacgcgat ccgtcgggat gtcgaggaat cgctcggcgc tgccgacgcc cgctatatcc gtcgcaccat cgctgcccag cgcacactgg agctcaccgg ccgcatcctg ctggcggcga gcaagaagcg ctccgcctgg tgggcgggcg cggtgacgct cggtgtggcc aagatcgtcg agaacatgga gatcggccac aacgtcatgc acggccagtg ggattggatg aacgatcccg agattcactc ctccagctgg gagtgggaca tggtggatcc ggccgagcac tggaagcaga ctcacaacta catccaccac aaatacacca acgtgctcgg catggacgac gatgtgggct acgggctgct gcgcgtcaca cgcgacaaga agtggaagcc attcaacctg ggcaacctgc tctacaacac gctgctgatg ctgctcttcg aatggggtgt tggcacacag catctggagc tgggaaaggt ggcgcagggg cgggccgaca aggaagaatt ccgggtcaag cgtcaggcgg cattgaccaa gatgggcaag caggtcgcca aggactacgt ggccttcccg gcgctcagcg cgctctcgcc cgcgggcagc taccgcaaga cggtcaccgc caacctgctg gccaatgtca ttcgcaacat ctggtccaac gcggtcatct tctgcggtca cttcccggac ggtgccgaga agttcacgaa gcaggacctg gagaacgaga cgcaaggaca gtggtacctg cgacagatgc tgggcagtgc caactttgac gcgggccccg cgatggcctt catgagtggc aatctgtgcc atcagatcga gcatcacctg ttcccggatc tgccgagcaa ccggctgtcg gcggtatcgg tgcgggtgcg tgagctgtgc gacaagtacg acctgccgta cacctcgggt ccattcctgg tgcaatacgc gaaggcatgg cgaaccatcg ccaagttgtc gctgccggac aagtacctga catacacggc cgacaacgcg caggagacgc gcagcgtcaa gatgttcgac gagttggaac cgcacgagcg gcgcgggctg aagtccgcgt tcgccgcggt gcgggcgcgg cgcaaggccc gtcacaaggt ctaa
Details for MyabA.18634.a.B1.GE37335
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMA ISDIQEFAHL TEADVEALGH ELDAIRRDVE ESLGAADARY IRRTIAAQRT LELTGRILLA ASKKRSAWWA GAVTLGVAKI VENMEIGHNV MHGQWDWMND PEIHSSSWEW DMVDPAEHWK QTHNYIHHKY TNVLGMDDDV GYGLLRVTRD KKWKPFNLGN LLYNTLLMLL FEWGVGTQHL ELGKVAQGRA DKEEFRVKRQ AALTKMGKQV AKDYVAFPAL SALSPAGSYR KTVTANLLAN VIRNIWSNAV IFCGHFPDGA EKFTKXDLEN ETQGQWYLRQ MLGSAXFDAG PAMXXXSGNL CXQIEHRXXX XRATGCRXXX XXCXXXXXXX XXXXXXXXAX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttgtnnn ntttangaag gaganatacc atggctcacc accaccacca ccatatggcg atttcggata tccaggaatt cgcgcacctc accgaggctg atgttgaggc gctcgggcac gagttggacg cgatccgtcg ggatgtcgag gaatcgctcg gcgctgccga cgcccgctat atccgtcgca ccatcgctgc ccagcgcaca ctggagctca ccggccgcat cctgctggcg gcgagcaaga agcgctccgc ctggtgggcg ggcgcggtga cgctcggtgt ggccaagatc gtcgagaaca tggagatcgg ccacaacgtc atgcacggcc agtgggattg gatgaacgat cccgagattc actcctccag ctgggagtgg gacatggtgg atccggccga gcactggaag cagactcaca actacatcca ccacaaatac accaacgtgc tcggcatgga cgacgatgtg ggctacgggc tgctgcgcgt cacacgcgac aagaagtgga agccattcaa cctgggcaac ctgctctaca acacgctgct gatgctgctc ttcgaatggg gtgttggcac acagcatctg gagctgggaa aggtggcgca ggggcgggcc gacaaggaag aattccgggt caagcgtcag gcggcattga ccaagatggg caagcaggtc gccaaggact acgtggcctt cccggcgctc agcgcgctct cgcccgcggg cagctaccgc aagacggtca ccgccaacct gctggccaat gtcattcgca acatctggtc caacgcggtc atcttctgcg gtcacttccc ggacggtgcc gagaagttca cgaagcanga cctggagaac gagacgcaag gacagtggta cctgcgacag atgctgggca gtgccnactt tgacgcgggc cccgcgatgg ncttnntgag tggcaatctg tgcnntcaga tcgagcatcg nnccnnnanc tgncgagcaa ccggntgtcg gcngnatnnn ngcngntgcn tganctgnnn nannntacna cnnnnnannn nnngnncnnn nngngcacnc nnnnntgnnn nntnnnnnnn nnnntncnnn antncnnann anannnnnnn nnnnnnnnnn cc
Expected Protein Sequence
MAHHHHHHMA ISDIQEFAHL TEADVEALGH ELDAIRRDVE ESLGAADARY IRRTIAAQRT LELTGRILLA ASKKRSAWWA GAVTLGVAKI VENMEIGHNV MHGQWDWMND PEIHSSSWEW DMVDPAEHWK QTHNYIHHKY TNVLGMDDDV GYGLLRVTRD KKWKPFNLGN LLYNTLLMLL FEWGVGTQHL ELGKVAQGRA DKEEFRVKRQ AALTKMGKQV AKDYVAFPAL SALSPAGSYR KTVTANLLAN VIRNIWSNAV IFCGHFPDGA EKFTKQDLEN ETQGQWYLRQ MLGSANFDAG PAMAFMSGNL CHQIEHHLFP DLPSNRLSAV SVRVRELCDK YDLPYTSGPF LVQYAKAWRT IAKLSLPDKY LTYTADNAQE TRSVKMFDEL EPHERRGLKS AFAAVRARRK ARHKV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga tttcggatat ccaggaattc gcgcacctca ccgaggctga tgttgaggcg ctcgggcacg agttggacgc gatccgtcgg gatgtcgagg aatcgctcgg cgctgccgac gcccgctata tccgtcgcac catcgctgcc cagcgcacac tggagctcac cggccgcatc ctgctggcgg cgagcaagaa gcgctccgcc tggtgggcgg gcgcggtgac gctcggtgtg gccaagatcg tcgagaacat ggagatcggc cacaacgtca tgcacggcca gtgggattgg atgaacgatc ccgagattca ctcctccagc tgggagtggg acatggtgga tccggccgag cactggaagc agactcacaa ctacatccac cacaaataca ccaacgtgct cggcatggac gacgatgtgg gctacgggct gctgcgcgtc acacgcgaca agaagtggaa gccattcaac ctgggcaacc tgctctacaa cacgctgctg atgctgctct tcgaatgggg tgttggcaca cagcatctgg agctgggaaa ggtggcgcag gggcgggccg acaaggaaga attccgggtc aagcgtcagg cggcattgac caagatgggc aagcaggtcg ccaaggacta cgtggccttc ccggcgctca gcgcgctctc gcccgcgggc agctaccgca agacggtcac cgccaacctg ctggccaatg tcattcgcaa catctggtcc aacgcggtca tcttctgcgg tcacttcccg gacggtgccg agaagttcac gaagcaggac ctggagaacg agacgcaagg acagtggtac ctgcgacaga tgctgggcag tgccaacttt gacgcgggcc ccgcgatggc cttcatgagt ggcaatctgt gccatcagat cgagcatcac ctgttcccgg atctgccgag caaccggctg tcggcggtat cggtgcgggt gcgtgagctg tgcgacaagt acgacctgcc gtacacctcg ggtccattcc tggtgcaata cgcgaaggca tggcgaacca tcgccaagtt gtcgctgccg gacaagtacc tgacatacac ggccgacaac gcgcaggaga cgcgcagcgt caagatgttc gacgagttgg aaccgcacga gcggcgcggg ctgaagtccg cgttcgccgc ggtgcgggcg cggcgcaagg cccgtcacaa ggtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat