MypaA.18627.a

DesA3_1 (Rv3229c ortholog)

CENTER ID: MypaA.18627.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.18627.a.B1.GE37489 full length 1 427
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3552
RefSeq: NP_962277.1
UniProt: Q73UM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAITDVDVFA HLTDADIENL AVELDAIRQD VEDSRGERDA RYIRRTIAAQ RALEVTGRLL LAASSRRSAW WAGTVTLGVA KIIENMEIGH NVMHGQWDWM NDPEIHSSTW EWDMSGSSKH WRYTHNFVHH KYTNILGMDD DVGYGMLRVT RDQRWKRFNL FNLVWNTLLA LLFEWGVGLQ HVEIGKILKR RMDHEEARQR IDEFLAKAGR QVVKDYVAFP ALTSLSPGAT FLSTLKANAM ANVIRNVWAN AVIFCGHFPD GAEKFTKTDM IGETKGQWYL RQMLGSANFE AGPVLRFMSG NLCHQIEHHL YPDLPSNRLH EISVRVRQVC DKYDLPYTTG SFLFQYAKTW RTLAKLSLPN KYLRDDADNA PETRSERMFA ELDPDFLGTD PATGRRRGLK SAIAAVRGWR RGKRASVARR TGGELAA
NT Sequence
atggcgatca cagacgtcga cgtattcgcc catctgacgg acgccgatat cgaaaacctg gccgttgagc tcgatgccat ccgccaggac gtggaggact cgcgcggcga gcgggacgcc cgctacatcc gccgcaccat cgcggcccag cgcgcgctgg aggtgaccgg ccggctgctg ctggccgcca gctcgcggcg ctcggcgtgg tgggcgggga cggtgaccct gggcgtggcc aagatcatcg agaacatgga gatcggccac aacgtcatgc acgggcagtg ggactggatg aacgaccccg agatccactc ctcgacgtgg gagtgggaca tgagcgggtc gtccaagcac tggcggtaca cccacaactt cgtgcaccac aagtacacca acattctcgg gatggacgac gacgtcggct acggcatgct gcgcgtcacc cgcgaccagc gctggaagcg cttcaacctg ttcaatctgg tgtggaacac gttgctggcc ttgctttttg aatggggagt gggcctgcag cacgtggaga tcggcaagat cctcaagcgc cggatggacc acgaggaagc ccggcagcgg atcgacgagt tcctggccaa ggcgggtcgc caggtggtca aggactacgt cgcgttcccg gcgctgacgt cactgtcgcc cggcgcgacc ttcctgtcca ccttgaaggc caacgcgatg gccaacgtga tccgcaacgt gtgggccaac gccgtcatct tctgcggcca tttcccggac ggcgcagaga aattcaccaa gaccgacatg atcggcgaga ccaagggaca gtggtacctg cgccagatgc tgggcagcgc caatttcgag gccggcccgg tgttgcggtt catgagcggc aacctgtgtc accagatcga gcaccacctg taccccgatc tgcccagcaa ccggttgcac gagatctcgg tgcgggtgcg tcaggtctgt gacaagtacg acttgcctta caccacaggg tctttcctgt tccagtacgc gaagacgtgg cgcacgctgg ccaagctctc gctgccgaac aagtatctgc gcgacgacgc cgacaacgct ccggagaccc gcagcgagcg gatgttcgct gagctggatc ccgatttcct gggcaccgat ccggctaccg gccgtcgccg cgggctcaag tccgcgatcg cggccgtgcg cggttggcgg cgcggcaagc gggcgtccgt ggcgcgtcgc accggcggtg agctggcggc ctaa
Details for MypaA.18627.a.B1.GE37489
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 50kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMA ITDVDVFAHL TDADIENLAV ELDAIRQDVE DSRGERDARY IRRTIAAQRA LEVTGRLLLA ASSRRSAWWA GTVTLGVAKI IENMEIGHNV MHGQWDWMND PEIHSSTWEW DMSGSSKHWR YTHNFVHHKY TNILGMDDDV GYGMLRVTRD QRWKRFNLFN LVWNTLLALL FEWGVGLQHV EIGKILKRRM DHEEARQRID EFLAKAGRQV VKDYVAFPAL TSLSPGATFL STLKANAMAN VIRNVXANAV IFCGHFPDGA XKFTKTDMIG ETXGTVVPAP DAGQRXFRGR PGVAXHEXXP VSXDRAXXVP XSAQXXXXXX XXXLXLXXYX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttnttta nctttaagaa ggaganatac catggctcac caccaccacc accatatggc gatcacagac gtcgacgtat tcgcccatct gacggacgcc gatatcgaaa acctggccgt tgagctcgat gccatccgcc aggacgtgga ggactcgcgc ggcgagcggg acgcccgcta catccgccgc accatcgcgg cccagcgcgc gctggaggtg accggccggc tgctgctggc cgccagctcg cggcgctcgg cgtggtgggc ggggacggtg accctgggcg tggccaagat catcgagaac atggagatcg gccacaacgt catgcacggg cagtgggact ggatgaacga ccccgagatc cactcctcga cgtgggagtg ggacatgagc gggtcgtcca agcactggcg gtacacccac aacttcgtgc accacaagta caccaacatt ctcgggatgg acgacgacgt cggctacggc atgctgcgcg tcacccgcga ccagcgctgg aagcgcttca acctgttcaa tctggtgtgg aacacgttgc tggccttgct ttttgaatgg ggagtgggcc tgcagcacgt ggagatcggc aagatcctca agcgccggat ggaccacgag gaagcccggc agcggatcga cgagttcctg gccaaggcgg gtcgccaggt ggtcaaggac tacgtcgcgt tcccggcgct gacgtcactg tcgcccggcg cgaccttcct gtccaccttg aaggccaacg cgatggccaa cgtgatccgc aacgtgtgng ccaacgccgt catcttctgc ggccatttcc cggacggcgc anagaaattc accaagaccg acatgatcgg cgagaccnan gggacagtgg tacctgcgcc agatgctggg cagcgccant ttcgaggncg gcccggtgtt gcgnntcatg agcnnnaacc tgtgtcanca gatcgagcnn canctgtacc cnnatctgcc cagcannnnn gnangagnnc tnnnnngnnn ctcngnctnn gannntacna ntnnnnnnnn nnggnnnttn cngntncant anncnnnann nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa nnannnnann nnnnnnnnnc nnnnnnnnnn nnnnnnc
Expected Protein Sequence
MAHHHHHHMA ITDVDVFAHL TDADIENLAV ELDAIRQDVE DSRGERDARY IRRTIAAQRA LEVTGRLLLA ASSRRSAWWA GTVTLGVAKI IENMEIGHNV MHGQWDWMND PEIHSSTWEW DMSGSSKHWR YTHNFVHHKY TNILGMDDDV GYGMLRVTRD QRWKRFNLFN LVWNTLLALL FEWGVGLQHV EIGKILKRRM DHEEARQRID EFLAKAGRQV VKDYVAFPAL TSLSPGATFL STLKANAMAN VIRNVWANAV IFCGHFPDGA EKFTKTDMIG ETKGQWYLRQ MLGSANFEAG PVLRFMSGNL CHQIEHHLYP DLPSNRLHEI SVRVRQVCDK YDLPYTTGSF LFQYAKTWRT LAKLSLPNKY LRDDADNAPE TRSERMFAEL DPDFLGTDPA TGRRRGLKSA IAAVRGWRRG KRASVARRTG GELAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga tcacagacgt cgacgtattc gcccatctga cggacgccga tatcgaaaac ctggccgttg agctcgatgc catccgccag gacgtggagg actcgcgcgg cgagcgggac gcccgctaca tccgccgcac catcgcggcc cagcgcgcgc tggaggtgac cggccggctg ctgctggccg ccagctcgcg gcgctcggcg tggtgggcgg ggacggtgac cctgggcgtg gccaagatca tcgagaacat ggagatcggc cacaacgtca tgcacgggca gtgggactgg atgaacgacc ccgagatcca ctcctcgacg tgggagtggg acatgagcgg gtcgtccaag cactggcggt acacccacaa cttcgtgcac cacaagtaca ccaacattct cgggatggac gacgacgtcg gctacggcat gctgcgcgtc acccgcgacc agcgctggaa gcgcttcaac ctgttcaatc tggtgtggaa cacgttgctg gccttgcttt ttgaatgggg agtgggcctg cagcacgtgg agatcggcaa gatcctcaag cgccggatgg accacgagga agcccggcag cggatcgacg agttcctggc caaggcgggt cgccaggtgg tcaaggacta cgtcgcgttc ccggcgctga cgtcactgtc gcccggcgcg accttcctgt ccaccttgaa ggccaacgcg atggccaacg tgatccgcaa cgtgtgggcc aacgccgtca tcttctgcgg ccatttcccg gacggcgcag agaaattcac caagaccgac atgatcggcg agaccaaggg acagtggtac ctgcgccaga tgctgggcag cgccaatttc gaggccggcc cggtgttgcg gttcatgagc ggcaacctgt gtcaccagat cgagcaccac ctgtaccccg atctgcccag caaccggttg cacgagatct cggtgcgggt gcgtcaggtc tgtgacaagt acgacttgcc ttacaccaca gggtctttcc tgttccagta cgcgaagacg tggcgcacgc tggccaagct ctcgctgccg aacaagtatc tgcgcgacga cgccgacaac gctccggaga cccgcagcga gcggatgttc gctgagctgg atcccgattt cctgggcacc gatccggcta ccggccgtcg ccgcgggctc aagtccgcga tcgcggccgt gcgcggttgg cggcgcggca agcgggcgtc cgtggcgcgt cgcaccggcg gtgagctggc ggcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat