MyulA.18627.a

Linoleoyl-CoA desaturase, DesA3 (Rv3229c ortholog)

CENTER ID: MyulA.18627.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.18627.a.B1.GE37681 full length 1 428
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3008
RefSeq: YP_906378.1
UniProt: A0PRD8

Orthologues for Rv3229c

Species % Identity % Coverage Clones Proteins Structures
marinum 88 100 0 0 0
ulcerans 88 100 1 0 0
paratuberculosis 85 100 1 0 0
marinum 81 100 0 0 0
ulcerans 80 100 1 0 0
thermoresistibile 80 98 0 0 0
fortuitum 79 96 1 0 0
abscessus 74 97 0 0 0
abscessus 69 97 1 0 0
paratuberculosis 62 20 0 0 0
paratuberculosis 60 30 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVITDVDVFA HLTDADIENL AIELDAIRQD IEDSRGERDA RYIRRTIAAQ RALEVSGRLM LAASSRRSAW WAGALTLGVA KIVENMEIGH NVMHGQWDWM NDPEIHSSSW EWDITGASKH WRYTHNFVHH KYTNILGMDD DVGYGMLRVT RDQRWKRYNI FNLVWNTLLA LGFEWGVALQ HLEIGKLLKG RLDRDTAETR VREFSRKAGR QVFKDYVAFP ALTSVSPGAT YRSTLTANVT ANVLRNVWTN AVIFCGHFPD GAEKFTKADM VGETKGQWYL RQMLGSANFE AGPALRFMSG NLCHQIEHHL YPDLPSNRLA EVSVRVREIC DKYDLPYTTG SFLVQYGKTW RTLAKLSLPN KYLRDNADDA PETRSERMFA DLEPGFAGAD PRTGRRRGLK SAIAAVRGRR RGKRVPEVRD SGTNDLAA
NT Sequence
atggtgatta ccgacgtcga cgtattcgcg catctgacgg acgccgatat cgagaatctg gcgattgagc tcgatgccat acgacaagac attgaagact ctcgcggtga gcgcgacgcc cgctatatcc gccgcaccat cgcggcgcag cgcgcgttgg aggtgagtgg ccgcctgatg ctggccgcca gctcgcgccg ttcggcgtgg tgggcggggg cattgaccct cggggtggcc aagatcgtcg agaacatgga gattggccac aatgtcatgc acggccaatg ggactggatg aacgatccgg agattcactc ctcgtcgtgg gagtgggaca tcaccggcgc gtccaagcat tggcgctaca cccacaactt cgtgcaccac aagtacacca acatcttggg catggacgac gatgtcggct acggcatgct tcgcgtcacc cgcgatcagc gctggaagcg ctacaacatt ttcaacttgg tgtggaacac cttgctggcg ctcggcttcg aatggggagt cgcgttgcag catttggaga tcggcaagct cctcaagggg cggctggacc gcgacaccgc cgagacccgg gtgcgcgagt tttcccgcaa ggccggtcgc caggtgttca aggactacgt ggcgttcccg gcgttgacgt cggtgtcgcc cggcgcgaca taccggtcca ccctgaccgc caacgtcacg gccaacgtgc tccgcaacgt gtggaccaac gccgtcatct tctgtgggca cttcccggat ggcgcagaga aattcaccaa ggctgacatg gtgggcgaga ccaaggggca gtggtacctg cggcagatgc tgggtagcgc caacttcgaa gccgggcccg cgctgcggtt catgagtggc aacctgtgtc atcaaatcga gcaccacctc tacccagatt tgccgagcaa ccggctggcc gaggtttcgg tgcgggtgcg cgagatctgc gacaagtacg acttgcccta caccacaggc tcgttcctgg tgcagtacgg caagacttgg cggaccctgg ccaagctgtc gctgccgaac aagtacctgc gtgacaacgc cgacgatgct ccggagaccc gcagtgagcg gatgttcgcg gacctggagc cgggcttcgc gggagccgat ccgcggaccg gccggcgccg tggcctcaag agtgcgattg ccgccgtgcg gggccgtcgg cgcggcaagc gtgtcccgga ggtaagggac agtggcacaa acgatctggc ggcctag
Details for MyulA.18627.a.B1.GE37681
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMV ITDVDVFAHL TDADIENLAI ELDAIRQDIE DSRGERDARY IRRTIAAQRA LEVSGRLMLA ASSRRSAWWA GALTLGVAKI VENMEIGHNV MHGQWDWMND PEIHSSSWEW DITGASKHWR YTHNFVHHKY TNILGMDDDV GYGMLRVTRD QRWKRYNIFN LVWNTLLALG FEWGVALQHL EIGKLLKGRL DRDTAETRVR EFSRKAGRQV FKDYVAFPAL TSVSPGATYR STLTANVTAN VLRNVWTNAV IFCGHFPDGA EKFTKADMVG ETKGQWYLRQ MLGSANFEAX PALRFMSGNL CHQIEHHLYP DLPSNRLAEV SXXGARDLRQ YDLPYTTGSF LVQYGRLGGX XXCRCRTSTX XTTXXCXXXP PXXMXXDXXX XPXPNXXXXA XXXXXXPXXX X
Validated NT Sequence
annncnctag anaattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgattacc gacgtcgacg tattcgcgca tctgacggac gccgatatcg agaatctggc gattgagctc gatgccatac gacaagacat tgaagactct cgcggtgagc gcgacgcccg ctatatccgc cgcaccatcg cggcgcagcg cgcgttggag gtgagtggcc gcctgatgct ggccgccagc tcgcgccgtt cggcgtggtg ggcgggggca ttgaccctcg gggtggccaa gatcgtcgag aacatggaga ttggccacaa tgtcatgcac ggccaatggg actggatgaa cgatccggag attcactcct cgtcgtggga gtgggacatc accggcgcgt ccaagcattg gcgctacacc cacaacttcg tgcaccacaa gtacaccaac atcttgggca tggacgacga tgtcggctac ggcatgcttc gcgtcacccg cgatcagcgc tggaagcgct acaacatttt caacttggtg tggaacacct tgctggcgct cggcttcgaa tggggagtcg cgttgcagca tttggagatc ggcaagctcc tcaaggggcg gctggaccgc gacaccgccg agacccgggt gcgcgagttt tcccgcaagg ccggtcgcca ggtgttcaag gactacgtgg cgttcccggc gttgacgtcg gtgtcgcccg gcgcgacata ccggtccacc ctgaccgcca acgtcacggc caacgtgctc cgcaacgtgt ggaccaacgc cgtcatcttc tgtgggcact tcccggatgg cgcagagaaa ttcaccaagg ctgacatggt gggcgagacc aaggggcagt ggtacctgcg gcagatgctg ggtagcgcca acttcgaagc cnggcccgcg ctgcggttca tgagtggcaa cctgtgtcat caaatcgagc accacctcta cccagatttg ccgagcaacc ggctggccga ggtttcgntg cngggtgcnc gagatctgcg acagtacgac ttgccctaca ccacaggctc gttcctggtg cagtacggca gacttggcgg ancctnnnnc tgtcgctgcc gaacnagtac ctgnntgaca acnnnannat gctncngana cccgccnnna ncgatgntcn nngacngnnn nnggnttccg gnnccgaatn nnnnnngnnn ggccnnnnna anntgnnntt nccccnnnnn nnncnnnng
Expected Protein Sequence
MAHHHHHHMV ITDVDVFAHL TDADIENLAI ELDAIRQDIE DSRGERDARY IRRTIAAQRA LEVSGRLMLA ASSRRSAWWA GALTLGVAKI VENMEIGHNV MHGQWDWMND PEIHSSSWEW DITGASKHWR YTHNFVHHKY TNILGMDDDV GYGMLRVTRD QRWKRYNIFN LVWNTLLALG FEWGVALQHL EIGKLLKGRL DRDTAETRVR EFSRKAGRQV FKDYVAFPAL TSVSPGATYR STLTANVTAN VLRNVWTNAV IFCGHFPDGA EKFTKADMVG ETKGQWYLRQ MLGSANFEAG PALRFMSGNL CHQIEHHLYP DLPSNRLAEV SVRVREICDK YDLPYTTGSF LVQYGKTWRT LAKLSLPNKY LRDNADDAPE TRSERMFADL EPGFAGADPR TGRRRGLKSA IAAVRGRRRG KRVPEVRDSG TNDLAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga ttaccgacgt cgacgtattc gcgcatctga cggacgccga tatcgagaat ctggcgattg agctcgatgc catacgacaa gacattgaag actctcgcgg tgagcgcgac gcccgctata tccgccgcac catcgcggcg cagcgcgcgt tggaggtgag tggccgcctg atgctggccg ccagctcgcg ccgttcggcg tggtgggcgg gggcattgac cctcggggtg gccaagatcg tcgagaacat ggagattggc cacaatgtca tgcacggcca atgggactgg atgaacgatc cggagattca ctcctcgtcg tgggagtggg acatcaccgg cgcgtccaag cattggcgct acacccacaa cttcgtgcac cacaagtaca ccaacatctt gggcatggac gacgatgtcg gctacggcat gcttcgcgtc acccgcgatc agcgctggaa gcgctacaac attttcaact tggtgtggaa caccttgctg gcgctcggct tcgaatgggg agtcgcgttg cagcatttgg agatcggcaa gctcctcaag gggcggctgg accgcgacac cgccgagacc cgggtgcgcg agttttcccg caaggccggt cgccaggtgt tcaaggacta cgtggcgttc ccggcgttga cgtcggtgtc gcccggcgcg acataccggt ccaccctgac cgccaacgtc acggccaacg tgctccgcaa cgtgtggacc aacgccgtca tcttctgtgg gcacttcccg gatggcgcag agaaattcac caaggctgac atggtgggcg agaccaaggg gcagtggtac ctgcggcaga tgctgggtag cgccaacttc gaagccgggc ccgcgctgcg gttcatgagt ggcaacctgt gtcatcaaat cgagcaccac ctctacccag atttgccgag caaccggctg gccgaggttt cggtgcgggt gcgcgagatc tgcgacaagt acgacttgcc ctacaccaca ggctcgttcc tggtgcagta cggcaagact tggcggaccc tggccaagct gtcgctgccg aacaagtacc tgcgtgacaa cgccgacgat gctccggaga cccgcagtga gcggatgttc gcggacctgg agccgggctt cgcgggagcc gatccgcgga ccggccggcg ccgtggcctc aagagtgcga ttgccgccgt gcggggccgt cggcgcggca agcgtgtccc ggaggtaagg gacagtggca caaacgatct ggcggcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at