SSGCID: Target Page

MytuD.11416.a

Chromosome partitioning protein

CENTER ID:	MytuD.11416.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.11416.a.B1.GE37758	full length		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3594
RefSeq:	NP_217729.1
RefSeq:	YP_006516685.1
TubercuList:	Rv3213c
MTB Network Portal:	Rv3213c
UniProt:	O05853

Orthologues for Rv3213c

Species	% Identity	% Coverage	Clones	Proteins	Structures
marinum	92	100	1	0	0
ulcerans	91	100	0	0	0
avium	90	100	1	1	0
paratuberculosis	90	100	1	1	0
fortuitum	88	98	1	0	0
thermoresistibile	87	99	1	2	0
smegmatis	87	98	1	2	3
abscessus	83	98	1	0	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDTRVLAVA NQKGGVAKTT TVASLGAAMV EKGRRVLLVD LDPQGCLTFS LGQDPDKLPV SVHEVLLGEV EPNAVLVTTM EGMTLLPANI DLAGAEAMLL MRAGREYALK RALAKFSDRF DVVIIDCPPS LGVLTLNGLT AADKAIVPLQ CEMLAHRGVG QFLRTVADVQ QITNPNLRLL GALPTLYDSR TTHTRDVLLD VADRYDLQVL APPIPRTVRF AEASASGSSV MAGRKNKGAV AYRELAQALL KHWKTGRPLP TFTVDL

NT Sequence

atgactgata cccgggtgct agcggtggcc aaccagaagg gcggggtggc caagacgact acggtcgcct cgctcggtgc ggcgatggtg gaaaagggac ggcgcgtgct gctcgtcgat ctggatccgc aaggttgtct gaccttctcc cttggtcaag atcccgacaa actaccggtg tctgtgcacg aggtgttgct cggtgaagtc gagcccaacg ccgtgctggt caccaccatg gagggaatga cgttgctgcc ggccaacatt gacttggcgg gcgccgaggc aatgctgctg atgcgggcag gccgtgagta cgcgctcaaa cgcgcgttgg ccaaattctc cgaccggttc gacgtggtca tcatcgactg tccgccgtcg ctgggtgtgc tcaccctcaa cgggctgacg gccgccgaca aggccatcgt gccgctgcag tgcgagatgc tggcgcaccg gggtgtcggc cagtttctgc ggacggtcgc cgatgtccag cagatcacca atccgaatct gcggttgttg ggtgcactgc cgacgctgta cgactctcga accacccaca cccgtgacgt gttgctcgat gtcgccgacc gctacgacct acaggtgctg gctccgccca tcccccgtac tgtgcgcttc gctgaggcca gcgcctcggg ctcgtcggtg atggctgggc gcaagaacaa aggcgcggtg gcctaccgcg agctggcgca ggcgctgctc aaacactgga agacaggtag gccgctgccc accttcacgg tggacctgta g

Details for MytuD.11416.a.B1.GE37758

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/7/2014
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

ggctcaccac caccaccacc atatgactga tacccgggtg ctagcggtgg ccaaccagaa gggcggggtg gccaagacga ctacggtcgc ctcgctcggt gcggcgatgg tggaaaaggg acggcgcgtg ctgctcgtcg atctggatcc gcaaggttgt ctgaccttct cccttggtca agatcccgac aaactaccgg tgtctgtgca cgaggtgttg ctcggtgaag tcgagcccaa cgccgtgctg gtcaccacca tggagggaat gacgttgctg ccggccaaca ttgacttggc gggcgccgag gcaatgctgc tgatgcgggc aggccgtgag tacgcgctca aacgcgcgtt ggccaaattc tccgaccggt tcgacgtggt catcatcgac tgtccgccgt cgctgggtgt gctcaccctc aacgggctga cggccgccga caaggccatc gtgccgctgc agtgcgagat gctggcgcac cggggtgtcg gccagtttct gcggacggtc gccgatgtcc agcagatcac caatccgaat ctgcggttgt tgggtgcact gccgacgctg tacgactctc gaaccaccca cacccgtgac gtgttgctcg atgtcgccga ccgctacgac ctacaggtgc tggctccgcc catcccccgt actgtgcgct tcgctgangc cagcgcctcg ggctcgtcgg tgatggctgg gcgcaagaac aaaggcgcgg tggcctaccg cgagctggcg canncgctgc tcaaacactg gaagacaggt aggccgctgc ccaccttcac ggtggacctg taantgagta anataggatc cggctgctac

Expected Protein Sequence

MAHHHHHHMT DTRVLAVANQ KGGVAKTTTV ASLGAAMVEK GRRVLLVDLD PQGCLTFSLG QDPDKLPVSV HEVLLGEVEP NAVLVTTMEG MTLLPANIDL AGAEAMLLMR AGREYALKRA LAKFSDRFDV VIIDCPPSLG VLTLNGLTAA DKAIVPLQCE MLAHRGVGQF LRTVADVQQI TNPNLRLLGA LPTLYDSRTT HTRDVLLDVA DRYDLQVLAP PIPRTVRFAE ASASGSSVMA GRKNKGAVAY RELAQALLKH WKTGRPLPTF TVDL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg atacccgggt gctagcggtg gccaaccaga agggcggggt ggccaagacg actacggtcg cctcgctcgg tgcggcgatg gtggaaaagg gacggcgcgt gctgctcgtc gatctggatc cgcaaggttg tctgaccttc tcccttggtc aagatcccga caaactaccg gtgtctgtgc acgaggtgtt gctcggtgaa gtcgagccca acgccgtgct ggtcaccacc atggagggaa tgacgttgct gccggccaac attgacttgg cgggcgccga ggcaatgctg ctgatgcggg caggccgtga gtacgcgctc aaacgcgcgt tggccaaatt ctccgaccgg ttcgacgtgg tcatcatcga ctgtccgccg tcgctgggtg tgctcaccct caacgggctg acggccgccg acaaggccat cgtgccgctg cagtgcgaga tgctggcgca ccggggtgtc ggccagtttc tgcggacggt cgccgatgtc cagcagatca ccaatccgaa tctgcggttg ttgggtgcac tgccgacgct gtacgactct cgaaccaccc acacccgtga cgtgttgctc gatgtcgccg accgctacga cctacaggtg ctggctccgc ccatcccccg tactgtgcgc ttcgctgagg ccagcgcctc gggctcgtcg gtgatggctg ggcgcaagaa caaaggcgcg gtggcctacc gcgagctggc gcaggcgctg ctcaaacact ggaagacagg taggccgctg cccaccttca cggtggacct gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat