MythA.00516.a

Cobyrinic acid a,c-diamide synthase (Rv3918c ortholog)

CENTER ID: MythA.00516.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00516.a.B1.GE37639 full length 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00516.a.B1.PS02019 full length 1 264

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.4434
UniProt: G7CN89

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVLAVANQ KGGVAKTTTV ASLGAAFAEK GIRVLLVDLD PQGCLTFSLG HDPDKLAVSV HEVLLGEVEP GAALVETAEG MTLLPANIDL AGAEAMLLMR AGREYALKRA LAKVSDDFEV IIIDCPPSLG VLTLNGLTAA QEAIVPLQCE TLAHRGVGQF LRTIDDVQQI TNPDLTLLGA LPTLYDSRTT HSRDVLLDVA DRYGLPVLAP PIPRTVRFAE ASASGASVLA GRKNKGALAY RELAEALLKH WETGEEMATF TPEL
NT Sequence
gtgacgcgag tacttgcggt cgccaaccag aagggcgggg tcgccaagac gaccacggtg gcgtcgctgg gcgcggcgtt cgcggagaag ggcatccgcg tgctgctggt cgacctcgat ccgcaggggt gtctgacctt ctcactcggc cacgatccgg acaagctggc ggtctcggtg cacgaagtgc tgctcgggga ggtcgaaccg ggcgccgcgc tggtggagac cgccgagggg atgacgctgc tgccggcgaa catcgacctg gccggcgccg aggcgatgct gctgatgcgg gcgggccgcg aatacgcgct caaacgcgcg ttggccaagg tcagcgacga cttcgaggtg atcatcatcg actgcccgcc ctcactgggc gtgctcaccc tcaacggcct gaccgcggcc caggaagcca tcgtgccgtt gcagtgcgaa accctggcgc accgcggcgt ggggcagttc ctgcgcacca tcgacgacgt ccagcagatc accaacccgg atctcacgct gctcggcgcg ctgcccaccc tctacgactc ccgcaccacg cacagccgtg atgtgctgct cgacgtcgcc gaccgctacg gcctgccggt gctggcgccg ccgatcccgc ggacggtgcg gttcgccgag gcttccgcgt cgggcgcctc ggtgctggcc ggccgcaaga acaagggtgc gctggcctat cgcgagctgg ccgaggccct gctcaagcac tgggagaccg gcgaggagat ggccaccttc accccggagc tctga
Details for MythA.00516.a.B1.GE37639
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TRVLAVANQK GGVAKTTTVA SLGAAFAEKG IRVLLVDLDP QGCLTFSLGH DPDKLAVSVH EVLLGEVEPG AALVETAEGM TLLPANIDLA GAEAMLLMRA GREYALKRAL AKVSDDFEVI IIDCPPSLGV LTLNGLTAAQ EAIVPLQCET LAHRGVGQFL RTIDDVQQIT NPDLTLLGAL PTLYDSRTTH SRDVLLDVAD RYGLPVLAPP IPRTVRFAEA SASGASVLAG RKNKGALAYR ELAEALLKHW ETGEEMATFT PEL
Validated NT Sequence
atcttactca cttagagctc cggggtgaag gtggccatct cctcgccggt ctcccagtgc ttgagcaggg cctcggccag ctcgcgatag gccagcgcac ccttgttctt gcggccggcc agcaccgagg cgcccgacgc ggaagcctcg gcgaaccgca ccgtccgcgg gatcggcggc gccagcaccg gcaggccgta gcggtcggcg acgtcgagca gcacatcacg gctgtgcgtg gtgcgggagt cgtagagggt gggcagcgcg ccgagcagcg tgagatccgg gttggtgatc tgctggacgt cgtcgatggt gcgcaggaac tgccccacgc cgcggtgcgc cagggtttcg cactgcaacg gcacgatggc ttcctgggcc gcggtcaggc cgttgagggt gagcacgccc agtgagggcg ggcagtcgat gatgatcacc tcgaagtcgt cgctgacctt ggccaacgcg cgtttgagcg cgtattcgcg gcccgcccgc atcagcagca tcgcctcggc gccggccagg tcgatgttcg ccggcagcag cgtcatcccc tcggcggtct ccaccagcgc ggcgcccggt tcgacctccc cgagcagcac ttcgtgcacc gagaccgcca gcttgtccgg atcgtggccg agtgagaagg tcagacaccc ctgcggatcg aggtcgacca gcagcacgcg gatgcccttc tccgcgaacg ccgcgcccag cgacgccacc gtggtcgtct tggcgacccc gcccttctgg ttggcgaccg caagtactcg cgtcaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT RVLAVANQKG GVAKTTTVAS LGAAFAEKGI RVLLVDLDPQ GCLTFSLGHD PDKLAVSVHE VLLGEVEPGA ALVETAEGMT LLPANIDLAG AEAMLLMRAG REYALKRALA KVSDDFEVII IDCPPSLGVL TLNGLTAAQE AIVPLQCETL AHRGVGQFLR TIDDVQQITN PDLTLLGALP TLYDSRTTHS RDVLLDVADR YGLPVLAPPI PRTVRFAEAS ASGASVLAGR KNKGALAYRE LAEALLKHWE TGEEMATFTP EL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacgc gagtacttgc ggtcgccaac cagaagggcg gggtcgccaa gacgaccacg gtggcgtcgc tgggcgcggc gttcgcggag aagggcatcc gcgtgctgct ggtcgacctc gatccgcagg ggtgtctgac cttctcactc ggccacgatc cggacaagct ggcggtctcg gtgcacgaag tgctgctcgg ggaggtcgaa ccgggcgccg cgctggtgga gaccgccgag gggatgacgc tgctgccggc gaacatcgac ctggccggcg ccgaggcgat gctgctgatg cgggcgggcc gcgaatacgc gctcaaacgc gcgttggcca aggtcagcga cgacttcgag gtgatcatca tcgactgccc gccctcactg ggcgtgctca ccctcaacgg cctgaccgcg gcccaggaag ccatcgtgcc gttgcagtgc gaaaccctgg cgcaccgcgg cgtggggcag ttcctgcgca ccatcgacga cgtccagcag atcaccaacc cggatctcac gctgctcggc gcgctgccca ccctctacga ctcccgcacc acgcacagcc gtgatgtgct gctcgacgtc gccgaccgct acggcctgcc ggtgctggcg ccgccgatcc cgcggacggt gcggttcgcc gaggcttccg cgtcgggcgc ctcggtgctg gccggccgca agaacaaggg tgcgctggcc tatcgcgagc tggccgaggc cctgctcaag cactgggaga ccggcgagga gatggccacc ttcaccccgg agctctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MythA.00516.a.B1.PS02019
PURIFICATION DATe: 3/11/2014
CONCENTRATION: 22.2mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TRVLAVANQK GGVAKTTTVA SLGAAFAEKG IRVLLVDLDP QGCLTFSLGH DPDKLAVSVH EVLLGEVEPG AALVETAEGM TLLPANIDLA GAEAMLLMRA GREYALKRAL AKVSDDFEVI IIDCPPSLGV LTLNGLTAAQ EAIVPLQCET LAHRGVGQFL RTIDDVQQIT NPDLTLLGAL PTLYDSRTTH SRDVLLDVAD RYGLPVLAPP IPRTVRFAEA SASGASVLAG RKNKGALAYR ELAEALLKHW ETGEEMATFT PEL
Validated NT Sequence
atcttactca cttagagctc cggggtgaag gtggccatct cctcgccggt ctcccagtgc ttgagcaggg cctcggccag ctcgcgatag gccagcgcac ccttgttctt gcggccggcc agcaccgagg cgcccgacgc ggaagcctcg gcgaaccgca ccgtccgcgg gatcggcggc gccagcaccg gcaggccgta gcggtcggcg acgtcgagca gcacatcacg gctgtgcgtg gtgcgggagt cgtagagggt gggcagcgcg ccgagcagcg tgagatccgg gttggtgatc tgctggacgt cgtcgatggt gcgcaggaac tgccccacgc cgcggtgcgc cagggtttcg cactgcaacg gcacgatggc ttcctgggcc gcggtcaggc cgttgagggt gagcacgccc agtgagggcg ggcagtcgat gatgatcacc tcgaagtcgt cgctgacctt ggccaacgcg cgtttgagcg cgtattcgcg gcccgcccgc atcagcagca tcgcctcggc gccggccagg tcgatgttcg ccggcagcag cgtcatcccc tcggcggtct ccaccagcgc ggcgcccggt tcgacctccc cgagcagcac ttcgtgcacc gagaccgcca gcttgtccgg atcgtggccg agtgagaagg tcagacaccc ctgcggatcg aggtcgacca gcagcacgcg gatgcccttc tccgcgaacg ccgcgcccag cgacgccacc gtggtcgtct tggcgacccc gcccttctgg ttggcgaccg caagtactcg cgtcaccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMT RVLAVANQKG GVAKTTTVAS LGAAFAEKGI RVLLVDLDPQ GCLTFSLGHD PDKLAVSVHE VLLGEVEPGA ALVETAEGMT LLPANIDLAG AEAMLLMRAG REYALKRALA KVSDDFEVII IDCPPSLGVL TLNGLTAAQE AIVPLQCETL AHRGVGQFLR TIDDVQQITN PDLTLLGALP TLYDSRTTHS RDVLLDVADR YGLPVLAPPI PRTVRFAEAS ASGASVLAGR KNKGALAYRE LAEALLKHWE TGEEMATFTP EL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACGC GAGTACTTGC GGTCGCCAAC CAGAAGGGCG GGGTCGCCAA GACGACCACG GTGGCGTCGC TGGGCGCGGC GTTCGCGGAG AAGGGCATCC GCGTGCTGCT GGTCGACCTC GATCCGCAGG GGTGTCTGAC CTTCTCACTC GGCCACGATC CGGACAAGCT GGCGGTCTCG GTGCACGAAG TGCTGCTCGG GGAGGTCGAA CCGGGCGCCG CGCTGGTGGA GACCGCCGAG GGGATGACGC TGCTGCCGGC GAACATCGAC CTGGCCGGCG CCGAGGCGAT GCTGCTGATG CGGGCGGGCC GCGAATACGC GCTCAAACGC GCGTTGGCCA AGGTCAGCGA CGACTTCGAG GTGATCATCA TCGACTGCCC GCCCTCACTG GGCGTGCTCA CCCTCAACGG CCTGACCGCG GCCCAGGAAG CCATCGTGCC GTTGCAGTGC GAAACCCTGG CGCACCGCGG CGTGGGGCAG TTCCTGCGCA CCATCGACGA CGTCCAGCAG ATCACCAACC CGGATCTCAC GCTGCTCGGC GCGCTGCCCA CCCTCTACGA CTCCCGCACC ACGCACAGCC GTGATGTGCT GCTCGACGTC GCCGACCGCT ACGGCCTGCC GGTGCTGGCG CCGCCGATCC CGCGGACGGT GCGGTTCGCC GAGGCTTCCG CGTCGGGCGC CTCGGTGCTG GCCGGCCGCA AGAACAAGGG TGCGCTGGCC TATCGCGAGC TGGCCGAGGC CCTGCTCAAG CACTGGGAGA CCGGCGAGGA GATGGCCACC TTCACCCCGG AGCTCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT