SSGCID: Target Page

MyabA.11416.b

Putative cobyrinic acid a,c-diamide synthase (Rv3918c ortholog)

CENTER ID:	MyabA.11416.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.11416.b.B1.GE37304	full length		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.3602
RefSeq:	YP_001704263.1
UniProt:	B1MF03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRVLAVANQ KGGVAKTTTV ASLGAALAQA GKKVLLVDLD PQGCLTFSLG QDPDRLGISV HEVLLGQADI KEALLTTTED LTLLPANIDL AGAEAMLLMR AGREYALKRA LESLGDQFDV VIIDCPPSLG VLTLNGLTAA DSVMVPLQCE TLAHRGVGQF LRTVTDVQQI TNPRLTLLGA LPTLYDSRTT HSRDVLLDVA DRYQLPVLAP PIPRTVRFAE ASASGASVLA GRKNKGALAY RELAQSLVKH WKSGKAMPTF TPPVD

NT Sequence

gtgactcgtg tgctggcggt agccaatcaa aagggtgggg tggccaaaac cacgacggtc gcgtcgctcg gcgcggcgtt ggcgcaggcc ggcaagaaag tgctgctggt cgacctggat ccgcagggtt gcctgacgtt ctcgctgggg caagatccgg accggctggg aatttcggtg catgaggtgc tgctggggca ggcggatatc aaggaagcgc tgctcaccac caccgaggat ttgacgctgc tacccgccaa tatcgacctg gcgggtgccg aggcgatgct gctgatgcgc gccggacgtg aatacgcgct gaagcgggct ctggaatcac tgggggatca gttcgacgtg gtgatcatcg actgcccgcc ctcgctgggc gtgctgacac tgaacggttt gacggctgcc gactcggtga tggtgccact gcagtgcgag acgctggcgc atcggggcgt tggccagttc ctgcggacgg tgaccgatgt ccagcagatc accaatccgc ggctgacgct attgggtgcg ctgcccacgc tgtacgactc gcggaccaca cacagccgcg acgtgctgct ggacgtggcc gatcggtacc agctgccggt gttggcaccg ccgattccca ggaccgtgcg gttcgcggag gcgagtgcct cgggtgcctc ggtgctggcg ggccgcaaga acaaaggtgc gctggcctat cgcgagctgg cacagagtct ggtgaagcac tggaagagcg gtaaggcgat gccgaccttc accccgccgg tcgactga

Details for MyabA.11416.b.B1.GE37304

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHHM VTRVLAVANQ KGGVAKTTTV ASLGAALAQA GKKVLLVDLD PQGCLTFSLG QDPDRLGISV HEVLLGQADI KEALLTTTED LTLLPANIDL AGAEAMLLMR AGREYALKRA LESLGDQFDV VIIDCPPSLG VLTLNGLTAA DSVMVPLQCE TLAHRGVGQF LRTVTDVQQI TNPRLTLLGA LPTLYDSRTT HSRDVLLDVA DRYQLPVLAP PIPRTVRFAE ASASGASVLA GRKNKGALAY RELAQSLVKH WKSGKAM

Validated NT Sequence

cggcatcgcc ttaccgctct tccagtgctt caccagactc tgtgccagct cgcgataggc cagcgcacct ttgttcttgc ggcccgccag caccgaggca cccgaggcac tcgcctccgc gaaccgcacg gtcctgggaa tcggcggtgc caacaccggc agctggtacc gatcggccac gtccagcagc acgtcgcggc tgtgtgtggt ccgcgagtcg tacagcgtgg gcagcgcacc caatagcgtc agccgcggat tggtgatctg ctggacatcg gtcaccgtcc gcaggaactg gccaacgccc cgatgcgcca gcgtctcgca ctgcagtggc accatcaccg agtcggcagc cgtcaaaccg ttcagtgtca gcacgcccag cgagggcggg cagtcgatga tcaccacgtc gaactgatcc cccagtgatt ccagagcccg cttcagcgcg tattcacgtc cggcgcgcat cagcagcatc gcctcggcac ccgccaggtc gatattggcg ggtagcagcg tcaaatcctc ggtggtggtg agcagcgctt ccttgatatc cgcctgcccc agcagcacct catgcaccga aattcccagc cggtccggat cttgccccag cgagaacgtc aggcaaccct gcggatccag gtcgaccagc agcactttct tgccggcctg cgccaacgcc gcgccgagcg acgcgaccgt cgtggttttg gccaccccac ccttttgatt ggctaccgcc agcacacgag tcaccatatg gtggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMT RVLAVANQKG GVAKTTTVAS LGAALAQAGK KVLLVDLDPQ GCLTFSLGQD PDRLGISVHE VLLGQADIKE ALLTTTEDLT LLPANIDLAG AEAMLLMRAG REYALKRALE SLGDQFDVVI IDCPPSLGVL TLNGLTAADS VMVPLQCETL AHRGVGQFLR TVTDVQQITN PRLTLLGALP TLYDSRTTHS RDVLLDVADR YQLPVLAPPI PRTVRFAEAS ASGASVLAGR KNKGALAYRE LAQSLVKHWK SGKAMPTFTP PVD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactc gtgtgctggc ggtagccaat caaaagggtg gggtggccaa aaccacgacg gtcgcgtcgc tcggcgcggc gttggcgcag gccggcaaga aagtgctgct ggtcgacctg gatccgcagg gttgcctgac gttctcgctg gggcaagatc cggaccggct gggaatttcg gtgcatgagg tgctgctggg gcaggcggat atcaaggaag cgctgctcac caccaccgag gatttgacgc tgctacccgc caatatcgac ctggcgggtg ccgaggcgat gctgctgatg cgcgccggac gtgaatacgc gctgaagcgg gctctggaat cactggggga tcagttcgac gtggtgatca tcgactgccc gccctcgctg ggcgtgctga cactgaacgg tttgacggct gccgactcgg tgatggtgcc actgcagtgc gagacgctgg cgcatcgggg cgttggccag ttcctgcgga cggtgaccga tgtccagcag atcaccaatc cgcggctgac gctattgggt gcgctgccca cgctgtacga ctcgcggacc acacacagcc gcgacgtgct gctggacgtg gccgatcggt accagctgcc ggtgttggca ccgccgattc ccaggaccgt gcggttcgcg gaggcgagtg cctcgggtgc ctcggtgctg gcgggccgca agaacaaagg tgcgctggcc tatcgcgagc tggcacagag tctggtgaag cactggaaga gcggtaaggc gatgccgacc ttcaccccgc cggtcgactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat