MymaA.00516.c

Soj/ParA-related protein (Rv3918c ortholog)

CENTER ID: MymaA.00516.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00516.c.B1.GE37190 full length 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1430
RefSeq: YP_001849658.1
UniProt: B2HEU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEIRVLAVA NQKGGVAKTT TVASLGAAMV DKGRRVLLVD LDPQGCLTFS LGHDPDKLTV SVHEVLLGEV EPSAALVTTM EGMTLLPANI DLAGAEAMLL MRAGREYALK RALAKLSDEF DVVIIDCPPS LGVLTLNGLT AADEVIVPLQ CETLAHRGVG QFLRTVSDVQ QITNPDLRLL GALPTLYDPR TTHTRDVLLD VADRYSLAVL APPIPRTVRF AEATASGSSV LAGRKNKGAL AYRELAQALL KHWKTGKPLP TFTVEP
NT Sequence
gtgacagaga tccgggtact ggcggtggcc aaccagaagg gcggagtggc caagacgacg acggtggcct ccctcggtgc ggcaatggtg gacaagggac ggcgggtgct gctggtcgat ttggatccgc aggggtgctt gaccttctcg ctgggccacg accccgacaa actgacggtg tccgtgcatg aggtgttgct cggggaagtc gagccgagcg ccgcgttggt gacgacgatg gagggaatga cgctgctgcc ggccaacatc gacttggccg gcgccgaggc gatgttgttg atgcgggccg gtcgtgagta cgcgctcaaa cgggcgctgg ccaaattgtc ggacgagttc gacgtcgtca tcatcgactg cccgccgtcg ctgggtgtgc tcacgctcaa cgggttgacg gccgccgacg aggtcatcgt cccgctgcaa tgcgagacgc tggcgcaccg cggcgtggga cagttcctgc gcacggtctc cgacgtgcag cagatcacca atccagacct acggttgctg ggcgccttgc cgacgctgta cgacccgcgt accacccaca cccgcgacgt gctgcttgac gtcgccgatc ggtacagcct ggcggtgctg gcgccaccga tcccacgcac cgtgcgattc gccgaagcca ccgcgtcggg ttcgtcggtg ctggccggac gcaagaacaa gggcgcgctg gcctatcgcg agctggcgca ggcactgctc aaacactgga agaccggcaa gcccctgccc accttcacgg tcgagcccta g
Details for MymaA.00516.c.B1.GE37190
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TEIRVLAVAN QKGGVAKTTT VASLGAAMVD KGRRVLLVDL DPQGCLTFSL GHDPDKLTVS VHEVLLGEVE PSAALVTTME GMTLLPANID LAGAEAMLLM RAGREYALKR ALAKLSDEFD VVIIDCPPSL GVLTLNGLTA ADEVIVPLQC ETLAHRGVGQ FLRTVSDVQQ ITNPDLRLLG ALPTLYDPRT THTRDVLLDV ADRYSLAVLA PPIPRTVRFA EATASGSSVL AGRKNKGALA YRELAQALLK HWKTGKPLPT FTVEP
Validated NT Sequence
tcttactcac ttagggctcg accgtgaagg tgggcagggg cttgccggtc ttccagtgtt tgagcagtgc ctgcgccagc tcgcgatagg ccagcgcgcc cttgttcttg cgtccggcca gcaccgacga acccgacgcg gtggcttcgg cgaatcgcac ggtgcgtggg atcggtggcg ccagcaccgc caggctgtac cgatcggcga cgtcaagcag cacgtcgcgg gtgtgggtgg tacgcgggtc gtacagcgtc ggcaaggcgc ccagcaaccg taggtctgga ttggtgatct gctgcacgtc ggagaccgtg cgcaggaact gtcccacgcc gcggtgcgcc agcgtctcgc attgcagcgg gacgatgacc tcgtcggcgg ccgtcaaccc gttgagcgtg agcacaccca gcgacggcgg gcagtcgatg atgacgacgt cgaactcgtc cgacaatttg gccagcgccc gtttgagcgc gtactcacga ccggcccgca tcaacaacat cgcctcggcg ccggccaagt cgatgttggc cggcagcagc gtcattccct ccatcgtcgt caccaacgcg gcgctcggct cgacttcccc gagcaacacc tcatgcacgg acaccgtcag tttgtcgggg tcgtggccca gcgagaaggt caagcacccc tgcggatcca aatcgaccag cagcacccgc cgtcccttgt ccaccattgc cgcaccgagg gaggccaccg tcgtcgtctt ggccactccg cccttctggt tggccaccgc cagtacccgg atctctgtca ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMT EIRVLAVANQ KGGVAKTTTV ASLGAAMVDK GRRVLLVDLD PQGCLTFSLG HDPDKLTVSV HEVLLGEVEP SAALVTTMEG MTLLPANIDL AGAEAMLLMR AGREYALKRA LAKLSDEFDV VIIDCPPSLG VLTLNGLTAA DEVIVPLQCE TLAHRGVGQF LRTVSDVQQI TNPDLRLLGA LPTLYDPRTT HTRDVLLDVA DRYSLAVLAP PIPRTVRFAE ATASGSSVLA GRKNKGALAY RELAQALLKH WKTGKPLPTF TVEP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacag agatccgggt actggcggtg gccaaccaga agggcggagt ggccaagacg acgacggtgg cctccctcgg tgcggcaatg gtggacaagg gacggcgggt gctgctggtc gatttggatc cgcaggggtg cttgaccttc tcgctgggcc acgaccccga caaactgacg gtgtccgtgc atgaggtgtt gctcggggaa gtcgagccga gcgccgcgtt ggtgacgacg atggagggaa tgacgctgct gccggccaac atcgacttgg ccggcgccga ggcgatgttg ttgatgcggg ccggtcgtga gtacgcgctc aaacgggcgc tggccaaatt gtcggacgag ttcgacgtcg tcatcatcga ctgcccgccg tcgctgggtg tgctcacgct caacgggttg acggccgccg acgaggtcat cgtcccgctg caatgcgaga cgctggcgca ccgcggcgtg ggacagttcc tgcgcacggt ctccgacgtg cagcagatca ccaatccaga cctacggttg ctgggcgcct tgccgacgct gtacgacccg cgtaccaccc acacccgcga cgtgctgctt gacgtcgccg atcggtacag cctggcggtg ctggcgccac cgatcccacg caccgtgcga ttcgccgaag ccaccgcgtc gggttcgtcg gtgctggccg gacgcaagaa caagggcgcg ctggcctatc gcgagctggc gcaggcactg ctcaaacact ggaagaccgg caagcccctg cccaccttca cggtcgagcc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat