MysmA.11416.b
Cobyrinic Acid a,c-diamide synthase (Rv3918c ortholog)
| CENTER ID: | MysmA.11416.b |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.11416.b.B1.GE37508 | full length | 1 | 265 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.11416.b.B1.PS01965 | full length | 1 | 265 |
Structures
4PFS
DEPOSITED: 4/30/2014
DETERMINATION: XRay
CLONE: MysmA.11416.b.B1.GE37508
PROTEIN: MysmA.11416.b.B1.PS01965
5IF9
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: MysmA.11416.b.B1.GE37508
PROTEIN: MysmA.11416.b.B1.PS01965
5IHP
DEPOSITED: 2/29/2016
DETERMINATION: XRay
CLONE: MysmA.11416.b.B1.GE37508
PROTEIN: MysmA.11416.b.B1.PS01965
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|246196.19.peg.1908 |
| RefSeq: | YP_886293.1 |
| UniProt: | A0QTQ5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVLAVANQ KGGVAKTTTV ASIGAALTEQ GRRVLLVDLD PQGCLTFSLG HDPDKLPVSV HEVLLGDVEP SAALVRTDEG MTLLPANIDL AGAEAMLLMR AGREYALKRA LAKLDGDFDV VIIDCPPSLG VLTLNGLTAA HDVIVPLQCE TLAHRGVGQF LRTISDVQQI TNPDLKLLGA LPTLYDSRTT HSRDVLLDVA DRYELPVLAP PIPRTVRFAE ASASGSSVLA GRKSKGAIAY REFADALLRH WKSGRKMPTF TPEVV
NT Sequence
gtgacgcgag tattagcggt cgccaatcaa aagggtgggg tagccaagac gacgactgtg gcgtccatcg gcgcggcgct caccgagcag gggcgccggg tgctgctcgt cgacctggac ccgcagggct gtctgacgtt ctcgctgggc cacgatcccg acaagctgcc cgtgtcggta cacgaggtgc tgctcggcga cgtcgagccc agtgccgcgc tggtccgcac cgacgagggg atgacgctgc tgccggccaa catcgatctg gccggcgccg aggccatgct cttgatgcgg gccgggaggg agtatgcgct caagcgcgcg ctggccaaac tcgatggcga cttcgacgtc gtgatcatcg actgcccgcc ctcgctgggt gtgctcacgc tcaacgggct caccgcagcg cacgacgtga tcgtgccgct gcagtgcgag acactggccc accgcggtgt cggccagttc ctgcgcacca tctccgacgt gcagcagatc accaacccgg acctgaaact gctgggggcg ctgcccacgc tgtacgactc acgcaccacg cacagccgcg acgtgttgct cgatgtcgcc gaccgctacg agcttcccgt gctggcgccg ccgattccgc gcacggtgcg gttcgccgag gccagtgcgt cgggatcctc ggtgcttgcc gggcgcaaga gcaaaggcgc catcgcctat cgcgagttcg ccgacgcgct gctgcggcac tggaagtccg gcaggaagat gcccacgttc accccagagg tcgtgtaa
Details for MysmA.11416.b.B1.GE37508
| HARVESTED ON: | 10/31/2013 |
| SEQUENCED ON: | 11/5/2013 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV TRVLAVANQK GGVAKTTTVA SIGAALTEQG RRVLLVDLDP QGCLTFSLGH DPDKLPVSVH EVLLGDVEPS AALVRTDEGM TLLPANIDLA GAEAMLLMRA GREYALKRAL AKLDGDFDVV IIDCPPSLGV LTLNGLTAAH DVIVPLQCET LAHRGVGQFL RTISDVQQIT NPDLKLLGAL PTLYDSRTTH SRDVLLDVAD RYELPVLAPP IPRTVRFAEA SASGSSVLAG RKSKGAIAYR EFADALLRHW KSGRKMPTFT PEVV
Validated NT Sequence
tcttactcac ttacacgacc tctggggtga acgtgggcat cttcctgccg gacttccagt gccgcagcag cgcgtcggcg aactcgcgat aggcgatggc gcctttgctc ttgcgcccgg caagcaccga ggatcccgac gcactggcct cggcgaaccg caccgtgcgc ggaatcggcg gcgccagcac gggaagctcg tagcggtcgg cgacatcgag caacacgtcg cggctgtgcg tggtgcgtga gtcgtacagc gtgggcagcg cccccagcag tttcaggtcc gggttggtga tctgctgcac gtcggagatg gtgcgcagga actggccgac accgcggtgg gccagtgtct cgcactgcag cggcacgatc acgtcgtgcg ctgcggtgag cccgttgagc gtgagcacac ccagcgaggg cgggcagtcg atgatcacga cgtcgaagtc gccatcgagt ttggccagcg cgcgcttgag cgcatactcc ctcccggccc gcatcaagag catggcctcg gcgccggcca gatcgatgtt ggccggcagc agcgtcatcc cctcgtcggt gcggaccagc gcggcactgg gctcgacgtc gccgagcagc acctcgtgta ccgacacggg cagcttgtcg ggatcgtggc ccagcgagaa cgtcagacag ccctgcgggt ccaggtcgac gagcagcacc cggcgcccct gctcggtgag cgccgcgccg atggacgcca cagtcgtcgt cttggctacc ccaccctttt gattggcgac cgctaatact cgcgtcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT RVLAVANQKG GVAKTTTVAS IGAALTEQGR RVLLVDLDPQ GCLTFSLGHD PDKLPVSVHE VLLGDVEPSA ALVRTDEGMT LLPANIDLAG AEAMLLMRAG REYALKRALA KLDGDFDVVI IDCPPSLGVL TLNGLTAAHD VIVPLQCETL AHRGVGQFLR TISDVQQITN PDLKLLGALP TLYDSRTTHS RDVLLDVADR YELPVLAPPI PRTVRFAEAS ASGSSVLAGR KSKGAIAYRE FADALLRHWK SGRKMPTFTP EVV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacgc gagtattagc ggtcgccaat caaaagggtg gggtagccaa gacgacgact gtggcgtcca tcggcgcggc gctcaccgag caggggcgcc gggtgctgct cgtcgacctg gacccgcagg gctgtctgac gttctcgctg ggccacgatc ccgacaagct gcccgtgtcg gtacacgagg tgctgctcgg cgacgtcgag cccagtgccg cgctggtccg caccgacgag gggatgacgc tgctgccggc caacatcgat ctggccggcg ccgaggccat gctcttgatg cgggccggga gggagtatgc gctcaagcgc gcgctggcca aactcgatgg cgacttcgac gtcgtgatca tcgactgccc gccctcgctg ggtgtgctca cgctcaacgg gctcaccgca gcgcacgacg tgatcgtgcc gctgcagtgc gagacactgg cccaccgcgg tgtcggccag ttcctgcgca ccatctccga cgtgcagcag atcaccaacc cggacctgaa actgctgggg gcgctgccca cgctgtacga ctcacgcacc acgcacagcc gcgacgtgtt gctcgatgtc gccgaccgct acgagcttcc cgtgctggcg ccgccgattc cgcgcacggt gcggttcgcc gaggccagtg cgtcgggatc ctcggtgctt gccgggcgca agagcaaagg cgccatcgcc tatcgcgagt tcgccgacgc gctgctgcgg cactggaagt ccggcaggaa gatgcccacg ttcaccccag aggtcgtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MysmA.11416.b.B1.PS01965
| PURIFICATION DATe: | 1/19/2014 |
| CONCENTRATION: | 9.61mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TRVLAVANQK GGVAKTTTVA SIGAALTEQG RRVLLVDLDP QGCLTFSLGH DPDKLPVSVH EVLLGDVEPS AALVRTDEGM TLLPANIDLA GAEAMLLMRA GREYALKRAL AKLDGDFDVV IIDCPPSLGV LTLNGLTAAH DVIVPLQCET LAHRGVGQFL RTISDVQQIT NPDLKLLGAL PTLYDSRTTH SRDVLLDVAD RYELPVLAPP IPRTVRFAEA SASGSSVLAG RKSKGAIAYR EFADALLRHW KSGRKMPTFT PEVV
Validated NT Sequence
tcttactcac ttacacgacc tctggggtga acgtgggcat cttcctgccg gacttccagt gccgcagcag cgcgtcggcg aactcgcgat aggcgatggc gcctttgctc ttgcgcccgg caagcaccga ggatcccgac gcactggcct cggcgaaccg caccgtgcgc ggaatcggcg gcgccagcac gggaagctcg tagcggtcgg cgacatcgag caacacgtcg cggctgtgcg tggtgcgtga gtcgtacagc gtgggcagcg cccccagcag tttcaggtcc gggttggtga tctgctgcac gtcggagatg gtgcgcagga actggccgac accgcggtgg gccagtgtct cgcactgcag cggcacgatc acgtcgtgcg ctgcggtgag cccgttgagc gtgagcacac ccagcgaggg cgggcagtcg atgatcacga cgtcgaagtc gccatcgagt ttggccagcg cgcgcttgag cgcatactcc ctcccggccc gcatcaagag catggcctcg gcgccggcca gatcgatgtt ggccggcagc agcgtcatcc cctcgtcggt gcggaccagc gcggcactgg gctcgacgtc gccgagcagc acctcgtgta ccgacacggg cagcttgtcg ggatcgtggc ccagcgagaa cgtcagacag ccctgcgggt ccaggtcgac gagcagcacc cggcgcccct gctcggtgag cgccgcgccg atggacgcca cagtcgtcgt cttggctacc ccaccctttt gattggcgac cgctaatact cgcgtcacca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMT RVLAVANQKG GVAKTTTVAS IGAALTEQGR RVLLVDLDPQ GCLTFSLGHD PDKLPVSVHE VLLGDVEPSA ALVRTDEGMT LLPANIDLAG AEAMLLMRAG REYALKRALA KLDGDFDVVI IDCPPSLGVL TLNGLTAAHD VIVPLQCETL AHRGVGQFLR TISDVQQITN PDLKLLGALP TLYDSRTTHS RDVLLDVADR YELPVLAPPI PRTVRFAEAS ASGSSVLAGR KSKGAIAYRE FADALLRHWK SGRKMPTFTP EVV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACGC GAGTATTAGC GGTCGCCAAT CAAAAGGGTG GGGTAGCCAA GACGACGACT GTGGCGTCCA TCGGCGCGGC GCTCACCGAG CAGGGGCGCC GGGTGCTGCT CGTCGACCTG GACCCGCAGG GCTGTCTGAC GTTCTCGCTG GGCCACGATC CCGACAAGCT GCCCGTGTCG GTACACGAGG TGCTGCTCGG CGACGTCGAG CCCAGTGCCG CGCTGGTCCG CACCGACGAG GGGATGACGC TGCTGCCGGC CAACATCGAT CTGGCCGGCG CCGAGGCCAT GCTCTTGATG CGGGCCGGGA GGGAGTATGC GCTCAAGCGC GCGCTGGCCA AACTCGATGG CGACTTCGAC GTCGTGATCA TCGACTGCCC GCCCTCGCTG GGTGTGCTCA CGCTCAACGG GCTCACCGCA GCGCACGACG TGATCGTGCC GCTGCAGTGC GAGACACTGG CCCACCGCGG TGTCGGCCAG TTCCTGCGCA CCATCTCCGA CGTGCAGCAG ATCACCAACC CGGACCTGAA ACTGCTGGGG GCGCTGCCCA CGCTGTACGA CTCACGCACC ACGCACAGCC GCGACGTGTT GCTCGATGTC GCCGACCGCT ACGAGCTTCC CGTGCTGGCG CCGCCGATTC CGCGCACGGT GCGGTTCGCC GAGGCCAGTG CGTCGGGATC CTCGGTGCTT GCCGGGCGCA AGAGCAAAGG CGCCATCGCC TATCGCGAGT TCGCCGACGC GCTGCTGCGG CACTGGAAGT CCGGCAGGAA GATGCCCACG TTCACCCCAG AGGTCGTGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT