MytuD.10611.c

Putative alcohol dehydrogenase D

CENTER ID: MytuD.10611.c
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.10611.c.B1.GE37757 full length 1 368
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3442
RefSeq: NP_217602.1
RefSeq: YP_006516547.1
TubercuList: Rv3086
MTB Network Portal: Rv3086
UniProt: P9WQB9

Orthologues for Rv3086

Species % Identity % Coverage Clones Proteins Structures
marinum 86 100 1 0 0
smegmatis 73 100 0 0 0
abscessus 51 99 1 0 0
marinum 47 99 1 0 0
smegmatis 47 99 0 0 0
ulcerans 46 99 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTTAAVLFE AGKPFELMEL DLDGPGPGEV LVKYTAAGLC HSDLHLTDGD LPPRFPIVGG HEGSGVIEEV GAGVTRVKPG DHVVCSFIPN CGTCRYCCTG RQNLCDMGAT ILEGCMPDGS FRFHSQGTDF GAMCMLGTFA ERATVSQHSV VKVDDWLPLE TAVLVGCGVP SGWGTAVNAG NLRAGDTAVI YGVGGLGINA VQGATAAGCK YVVVVDPVAF KRETALKFGA THAFADAASA AAKVDELTWG QGADAALILV GTVDDEVVSA ATAVIGKGGT VVITGLADPA KLTVHVSGTD LTLHEKTIKG SLFGSCNPQY DIVRLLRLYD AGQLMLDELV TTTYNLEQVN QGYQDLRDGK NIRGVIVH
NT Sequence
atgaaaacca ccgcggcggt actgttcgag gcgggcaaac cgttcgagct gatggagctc gatctcgacg ggccgggtcc gggcgaggtg ttggtcaaat acaccgccgc cgggctgtgc cattccgacc tgcacctcac cgatggtgat ttaccaccgc ggttcccgat cgtgggcggc cacgaagggt ccggggtcat cgaggaggtg ggtgccggcg tcaccagggt caagcccgga gaccacgtgg tgtgcagctt catcccgaac tgcgggactt gccgctactg ctgcaccggc cggcagaacc tgtgcgacat gggggccacc atcctggagg gctgcatgcc ggacggcagt ttccgattcc attcccaggg aacagatttc ggcgccatgt gcatgctggg cacgttcgcc gagcgggcca ccgtctcgca gcattcggtg gtgaaggtgg acgactggct gccactggaa accgcggtgc tggtgggctg cggcgtgccg tccggttggg gcaccgcggt caatgccgga aacctgcggg ccggcgacac cgccgtcatc tacggcgtcg gcggcctggg catcaacgcg gtccagggcg cgaccgccgc cggctgtaag tacgtcgtgg tggtggaccc ggtggctttc aagcgcgaga ccgcgctcaa gttcggcgcc acccatgcct tcgccgacgc cgccagcgcg gcggccaagg tcgacgaact cacctggggg cagggcgccg acgcggcgct gatcctggtg ggcaccgtcg acgacgaggt ggtctcggcc gcgaccgcgg tgatcggcaa gggcggcacc gtcgtcatca ccgggctggc ggacccggcc aaactcaccg tgcacgtctc cggaaccgat ttgacgctgc acgagaaaac gatcaagggc tcgctgttcg gttcctgcaa tccgcaatac gacatcgtgc ggctgctgcg cctctacgac gccggccagc tgatgctgga cgaactcgtg accaccacct acaacctcga acaggtgaac cagggctacc aggatctgcg ggacggcaag aacattcggg gcgtgatcgt gcactga
Details for MytuD.10611.c.B1.GE37757
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 39kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMK TTAAVLFEAG KPFELMELDL DGPGPGEVLV KYTAAGLCHS DLHLTDGDLP PRFPIVGGHE GSGVIEEVGA GVTRVKPGDH VVCSFIPNCG TCRYCCTGRQ NLCDMGATIL EGCMPDGSFR FHSQGTDFGA MCMLGTFAER ATVSQHSVVK VDDWLPLETA VLVGCGVPSG WGTAVNAGNL RAGDTAVIYG VGGLGINAVQ GATAAGCKYV VVVDPVAFKR ETALKFGATH AFADAASAAA KVDELTWGQG ADAALILVGT VDDEVVSAAT AVIGKGGTVV ITGLADPAKL TVHVSXTD
Validated NT Sequence
cnctagntat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa ccaccgcggc ggtactgttc gaggcgggca aaccgttcga gctgatggag ctcgatctcg acgggccggg tccgggcgag gtgttggtca aatacaccgc cgccgggctg tgccattccg acctgcacct caccgatggt gatttaccac cgcggttccc gatcgtgggc ggccacgaag ggtccggggt catcgaggag gtgggtgccg gcgtcaccag ggtcaagccc ggagaccacg tggtgtgcag cttcatcccg aactgcggga cttgccgcta ctgctgcacc ggccggcaga acctgtgcga catgggggcc accatcctgg agggctgcat gccggacggc agtttccgat tccattccca gggaacagat ttcggcgcca tgtgcatgct gggcacgttc gccgagcggg ccaccgtctc gcagcattcg gtggtgaagg tggacgactg gctgccactg gaaaccgcgg tgctggtggg ctgcggcgtg ccgtccggtt ggggcaccgc ggtcaatgcc ggaaacctgc gggccggcga caccgccgtc atctacggcg tcggcggcct gggcatcaac gcggtccagg gcgcgaccgc cgccggctgt aagtacgtcg tggtggtgga cccggtggct ttcaagcgcg agaccgcgct caagttcggc gccacccatg ccttcgccga cgccgccagc gcggcggcca aggtcgacga actcacctgg gggcagggcg ccgacgcggc gctgatcctg gtgggcaccg tcgacgacga ggtggtctcg gccgcgaccg cggtgatcgg caagggcggc accgtcgtca tcaccgggct ggcggacccg gccaaactca ccgtgcacgt ctccngaacc gattgacgct gcacgagaaa acgatcaagg gctcgctgtt ccgntntgca atcnnaatac gacatcntgc ggctgctgcg ctctacgang ncggcnanct gatgntggac gaantcntga cnnccnccta nnnctcnaan a
Expected Protein Sequence
MAHHHHHHMK TTAAVLFEAG KPFELMELDL DGPGPGEVLV KYTAAGLCHS DLHLTDGDLP PRFPIVGGHE GSGVIEEVGA GVTRVKPGDH VVCSFIPNCG TCRYCCTGRQ NLCDMGATIL EGCMPDGSFR FHSQGTDFGA MCMLGTFAER ATVSQHSVVK VDDWLPLETA VLVGCGVPSG WGTAVNAGNL RAGDTAVIYG VGGLGINAVQ GATAAGCKYV VVVDPVAFKR ETALKFGATH AFADAASAAA KVDELTWGQG ADAALILVGT VDDEVVSAAT AVIGKGGTVV ITGLADPAKL TVHVSGTDLT LHEKTIKGSL FGSCNPQYDI VRLLRLYDAG QLMLDELVTT TYNLEQVNQG YQDLRDGKNI RGVIVH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa ccaccgcggc ggtactgttc gaggcgggca aaccgttcga gctgatggag ctcgatctcg acgggccggg tccgggcgag gtgttggtca aatacaccgc cgccgggctg tgccattccg acctgcacct caccgatggt gatttaccac cgcggttccc gatcgtgggc ggccacgaag ggtccggggt catcgaggag gtgggtgccg gcgtcaccag ggtcaagccc ggagaccacg tggtgtgcag cttcatcccg aactgcggga cttgccgcta ctgctgcacc ggccggcaga acctgtgcga catgggggcc accatcctgg agggctgcat gccggacggc agtttccgat tccattccca gggaacagat ttcggcgcca tgtgcatgct gggcacgttc gccgagcggg ccaccgtctc gcagcattcg gtggtgaagg tggacgactg gctgccactg gaaaccgcgg tgctggtggg ctgcggcgtg ccgtccggtt ggggcaccgc ggtcaatgcc ggaaacctgc gggccggcga caccgccgtc atctacggcg tcggcggcct gggcatcaac gcggtccagg gcgcgaccgc cgccggctgt aagtacgtcg tggtggtgga cccggtggct ttcaagcgcg agaccgcgct caagttcggc gccacccatg ccttcgccga cgccgccagc gcggcggcca aggtcgacga actcacctgg gggcagggcg ccgacgcggc gctgatcctg gtgggcaccg tcgacgacga ggtggtctcg gccgcgaccg cggtgatcgg caagggcggc accgtcgtca tcaccgggct ggcggacccg gccaaactca ccgtgcacgt ctccggaacc gatttgacgc tgcacgagaa aacgatcaag ggctcgctgt tcggttcctg caatccgcaa tacgacatcg tgcggctgct gcgcctctac gacgccggcc agctgatgct ggacgaactc gtgaccacca cctacaacct cgaacaggtg aaccagggct accaggatct gcgggacggc aagaacattc ggggcgtgat cgtgcactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at