MyulA.10611.e

Zinc-type alcohol dehydrogenase AdhD

CENTER ID: MyulA.10611.e
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.10611.e.A1.GE29968 Full length( MyulA.10611.e ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.5622
RefSeq: YP_908242.1
UniProt: A0PWQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSRAAILYG YGHPWTVEEF ELDPPRAGEV LLQLSATGLC HSDEHIRRGN LAPPPETLRS FGLPTMSPTI GGHEGSGVVL EVGEGVTRFA PGDHVVTSFI AVCGQCRWCA TGMEYLCDTG YGTLAPGMPT DGTFRHHSLD GRDLGHVSKI GPFAEHTVVS ADSLVKIDQT FPLVPAALLA CAIPTRYGSA ARRAQVRGGD IAVVIGAGGI GTAAIQGARI QGATRIIAVD PVEFERKSAL TFGATHTAAT TDEAVETVRE LTRGVMADVV VVSPATIGPA DVAAALSLTR KGGTCVLTGL PDPAMHSIAV GLQDFVLMNK TLCGTVFGSC NPKSDIPLLA TMYQSGQLLL DAMITKRYSL HSINDAYADL VAGELIRGVI DFSLS
NT Sequence
ATGAGAAGCC GCGCGGCCAT CCTTTATGGG TACGGTCACC CCTGGACCGT CGAAGAATTC GAGCTCGACC CGCCGCGCGC CGGCGAGGTG CTGCTGCAAC TGAGCGCGAC CGGACTGTGC CACTCCGATG AACACATCCG ACGCGGCAAC CTCGCGCCAC CACCGGAGAC ACTGCGCTCT TTCGGGCTGC CGACCATGTC CCCGACCATC GGCGGCCATG AAGGATCCGG CGTCGTGCTC GAGGTCGGCG AGGGGGTGAC CCGGTTCGCA CCCGGCGACC ATGTGGTGAC GTCTTTCATC GCGGTTTGCG GCCAGTGCCG CTGGTGCGCA ACGGGAATGG AATACCTATG CGATACCGGA TACGGCACCT TGGCACCCGG AATGCCCACC GACGGTACGT TCCGTCATCA CAGCCTCGAT GGACGCGACC TCGGCCACGT GTCCAAGATC GGCCCGTTCG CCGAGCATAC GGTGGTCTCG GCCGATTCGC TGGTCAAGAT TGATCAGACG TTCCCGCTGG TGCCGGCAGC CCTGCTGGCC TGTGCGATAC CGACCAGATA TGGCTCGGCG GCACGACGCG CTCAGGTCCG CGGCGGCGAC ATCGCCGTGG TGATCGGCGC GGGCGGCATC GGAACAGCGG CCATCCAAGG CGCCCGTATC CAGGGAGCGA CCCGCATCAT CGCGGTCGAT CCCGTTGAGT TCGAGCGCAA ATCCGCGCTG ACCTTTGGGG CCACTCATAC CGCGGCGACC ACGGACGAGG CCGTTGAGAC GGTGCGCGAG CTAACTCGCG GCGTGATGGC GGATGTGGTC GTGGTATCCC CCGCCACGAT CGGCCCGGCC GACGTCGCGG CGGCACTGTC GCTGACCCGC AAGGGCGGAA CCTGCGTGCT CACCGGTCTG CCGGACCCCG CGATGCACTC CATCGCCGTC GGTCTTCAAG ACTTCGTGCT GATGAATAAG ACGCTGTGTG GCACGGTCTT TGGCTCGTGC AACCCAAAGA GTGACATTCC GCTGCTGGCC ACGATGTACC AGTCGGGGCA GTTACTCTTG GACGCGATGA TCACCAAGCG CTACTCGCTC CACAGCATCA ACGACGCCTA CGCCGATCTG GTTGCCGGCG AGTTGATCCG CGGGGTCATC GATTTCAGTC TCAGC
Details for MyulA.10611.e.A1.GE29968
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MRSRAAILYG YGHPWTVEEF ELDPPRAGEV LLQLSATGLC HSDEHIRRGN LAPPPETLRS FGLPTMSPTI GGHEGSGVVL EVGEGVTRFA PGDHVVTSFI AVCGQCRWCA TGMEYLCDTG YGTLAPGMPT DGTFRHHSLD GRDLGHVSKI GPFAEHTVVS ADSLVKIDQT FPLVPAALLA CAIPTRYGSA ARRAQVRGGD IAVVIGAGGI GTAAIQGARI QGATRIIAVD PVEFERKSAL TFGATHTAAT TDEAVETVRE LTRGVMADVV VVSPATIGPA DVAAALSLTR KGGTCVLTGL PDPAMHSIAV GLQDFVLMNK TLCGTVFGSC NPKSDIPLLA TMYQSGQLLL DAMITKRYSL HSINDAYADL VAGELIRGVI DFS
Validated NT Sequence
taccctggaa gctcagaccc agggtcctgg ttcgatgaga agccgcgcgg ccatccttta tgggtacggt cacccctgga ccgtcgaaga attcgagctc gacccgccgc gcgccggcga ggtgctgctg caactgagcg cgaccggact gtgccactcc gatgaacaca tccgacgcgg caacctcgcg ccaccaccgg agacactgcg ctctttcggg ctgccgacca tgtccccgac catcggcggc catgaaggat ccggcgtcgt gctcgaggtc ggcgaggggg tgacccggtt cgcacccggc gaccatgtgg tgacgtcttt catcgcggtt tgcggccagt gccgctggtg cgcaacggga atggaatacc tatgcgatac cggatacggc accttggcac ccggaatgcc caccgacggt acgttccgtc atcacagcct cgatggacgc gacctcggcc acgtgtccaa gatcggcccg ttcgccgagc atacggtggt ctcggccgat tcgctggtca agattgatca gacgttcccg ctggtgccgg cagccctgct ggcctgtgcg ataccgacca gatatggctc ggcggcacga cgcgctcagg tccgcggcgg cgacatcgcc gtggtgatcg gcgcgggcgg catcggaaca gcggccatcc aaggcgcccg tatccaggga gcgacccgca tcatcgcggt cgatcccgtt gagttcgagc gcaaatccgc gctgaccttt ggggccactc ataccgcggc gaccacggac gaggccgttg agacggtgcg cgagctaact cgcggcgtga tggcggatgt ggtcgtggta tcccccgcca cgatcggccc ggccgacgtc gcggcggcac tgtcgctgac ccgcaagggc ggaacctgcg tgctcaccgg tctgccggac cccgcgatgc actccatcgc cgtcggtctt caagacttcg tgctgatgaa taagacgctg tgtggcacgg tctttggctc gtgcaaccca aagagtgaca ttccgctgct ggccacgatg taccagtcgg ggcagttact cttggacgcg atgatcacca agcgctactc gctccacagc atcaacgacg cctacgccga tctggttgcc ggcgagttga tccgcggggt catcgatttc agtctc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRSRAAILY GYGHPWTVEE FELDPPRAGE VLLQLSATGL CHSDEHIRRG NLAPPPETLR SFGLPTMSPT IGGHEGSGVV LEVGEGVTRF APGDHVVTSF IAVCGQCRWC ATGMEYLCDT GYGTLAPGMP TDGTFRHHSL DGRDLGHVSK IGPFAEHTVV SADSLVKIDQ TFPLVPAALL ACAIPTRYGS AARRAQVRGG DIAVVIGAGG IGTAAIQGAR IQGATRIIAV DPVEFERKSA LTFGATHTAA TTDEAVETVR ELTRGVMADV VVVSPATIGP ADVAAALSLT RKGGTCVLTG LPDPAMHSIA VGLQDFVLMN KTLCGTVFGS CNPKSDIPLL ATMYQSGQLL LDAMITKRYS LHSINDAYAD LVAGELIRGV IDFSL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagaagccgc gcggccatcc tttatgggta cggtcacccc tggaccgtcg aagaattcga gctcgacccg ccgcgcgccg gcgaggtgct gctgcaactg agcgcgaccg gactgtgcca ctccgatgaa cacatccgac gcggcaacct cgcgccacca ccggagacac tgcgctcttt cgggctgccg accatgtccc cgaccatcgg cggccatgaa ggatccggcg tcgtgctcga ggtcggcgag ggggtgaccc ggttcgcacc cggcgaccat gtggtgacgt ctttcatcgc ggtttgcggc cagtgccgct ggtgcgcaac gggaatggaa tacctatgcg ataccggata cggcaccttg gcacccggaa tgcccaccga cggtacgttc cgtcatcaca gcctcgatgg acgcgacctc ggccacgtgt ccaagatcgg cccgttcgcc gagcatacgg tggtctcggc cgattcgctg gtcaagattg atcagacgtt cccgctggtg ccggcagccc tgctggcctg tgcgataccg accagatatg gctcggcggc acgacgcgct caggtccgcg gcggcgacat cgccgtggtg atcggcgcgg gcggcatcgg aacagcggcc atccaaggcg cccgtatcca gggagcgacc cgcatcatcg cggtcgatcc cgttgagttc gagcgcaaat ccgcgctgac ctttggggcc actcataccg cggcgaccac ggacgaggcc gttgagacgg tgcgcgagct aactcgcggc gtgatggcgg atgtggtcgt ggtatccccc gccacgatcg gcccggccga cgtcgcggcg gcactgtcgc tgacccgcaa gggcggaacc tgcgtgctca ccggtctgcc ggaccccgcg atgcactcca tcgccgtcgg tcttcaagac ttcgtgctga tgaataagac gctgtgtggc acggtctttg gctcgtgcaa cccaaagagt gacattccgc tgctggccac gatgtaccag tcggggcagt tactcttgga cgcgatgatc accaagcgct actcgctcca cagcatcaac gacgcctacg ccgatctggt tgccggcgag ttgatccgcg gggtcatcga tttcagtctc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa