MymaA.10611.c

Zinc-containing alcohol dehydrogenase NAD-dependent, AdhD_1

CENTER ID: MymaA.10611.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.10611.c.A1.GE30238 Full length( MymaA.10611.c ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.160
RefSeq: YP_001848452.1
UniProt: B2HJC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTTAAVLLE AGKPFEIMEL ELDGPRVGEV LIKYTAAGLC HSDLHLTDGD LPPRYPIVGG HEGSGVIEEV GPGVSKVKPG DHVVCSFIPN CGTCRYCSTG RQNLCDMGAT ILEGCMSDGS FRFHRKGVDF GAMCMLGTFS ERATISQHSV VKIDDWLPLT TAVLVGCGVP TGWGTSVYAG GVRPGDTTVI YGAGGLGINA VQGAVSAGAK HVVVVDPVAF KRDTALKFGA THAFPDAASA AAKVNEITWG QGADQALILV GTVDTEVVSA AAAVIGKGGT VVITGLANPE KLTVQLSGLD LTLNEKTIKG TLFGSANPQY DIVRLLRLYD AGQLKLDELI TTQYRLEDVN QGYQDLRDGK NIRGVIVHD
NT Sequence
ATGAAGACCA CAGCAGCCGT GTTGCTGGAA GCGGGCAAGC CCTTCGAGAT CATGGAACTC GAACTCGACG GCCCCCGCGT CGGCGAAGTA CTGATCAAAT ACACCGCCGC GGGTTTGTGC CACTCAGACC TGCACCTGAC CGACGGCGAC CTGCCACCGC GGTATCCGAT TGTCGGCGGC CACGAAGGAT CGGGCGTCAT CGAGGAAGTC GGTCCCGGGG TCAGCAAGGT CAAACCCGGG GACCACGTCG TGTGCTCCTT CATCCCCAAC TGCGGCACCT GCCGATACTG TTCCACCGGA CGGCAGAACC TTTGCGACAT GGGCGCGACC ATCCTCGAAG GATGCATGAG CGATGGCAGC TTCCGCTTCC ACCGCAAGGG GGTGGACTTC GGTGCGATGT GCATGCTCGG CACCTTCTCC GAGCGCGCCA CCATCTCGCA GCACTCGGTG GTCAAGATCG ACGACTGGTT GCCACTGACC ACCGCGGTGC TGGTGGGCTG CGGAGTTCCG ACCGGCTGGG GTACCTCGGT ATATGCCGGG GGCGTGCGGC CCGGCGACAC GACGGTCATC TACGGCGCGG GCGGGCTGGG CATCAACGCC GTACAAGGAG CGGTGAGCGC CGGAGCCAAA CACGTCGTGG TGGTCGATCC GGTTGCGTTC AAGCGGGACA CCGCGCTGAA ATTCGGCGCT ACCCATGCCT TCCCCGACGC GGCCAGCGCG GCCGCGAAGG TCAACGAAAT CACCTGGGGC CAAGGCGCGG ACCAAGCGCT CATTCTGGTC GGCACGGTCG ACACGGAAGT GGTCAGTGCC GCGGCTGCGG TGATCGGCAA GGGCGGCACC GTGGTCATCA CCGGGTTGGC GAATCCGGAG AAGCTCACGG TGCAACTGTC GGGCCTGGAT CTGACCCTCA ACGAGAAGAC GATCAAGGGC ACACTGTTCG GATCGGCCAA TCCCCAATAC GACATCGTGC GCCTACTCCG GCTCTACGAC GCCGGCCAAC TCAAACTCGA CGAGCTGATC ACCACCCAAT ATCGACTCGA AGATGTCAAC CAGGGCTATC AAGATCTGCG CGACGGCAAG AACATTCGCG GCGTGATCGT CCACGAC
Details for MymaA.10611.c.A1.GE30238
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKTTAAVLL EAGKPFEIME LELDGPRVGE VLIKYTAAGL CHSDLHLTDG DLPPRYPIVG GHEGSGVIEE VGPGVSKVKP GDHVVCSFIP NCGTCRYCST GRQNLCDMGA TILEGCMSDG SFRFHRKGVD FGAMCMLGTF SERATISQHS VVKIDDWLPL TTAVLVGCGV PTGWGTSVYA GGVRPGDTTV IYGAGGLGIN AVQGAVSAGA KHVVVVDPVA FKRDTALKFG ATHAFPDAAS AAAXVNEITW GXXADQALIL VGTVXTEVVS AAAAVXXXXX PXXSXGXXNP XKLTXXLSXX XXXLNXKTIX GTLXXRXXPX TXXXRXLXXX RXXXNSTX
Validated NT Sequence
attttntnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaga ccacagcagc cgtgttgctg gaagcgggca agcccttcga gatcatggaa ctcgaactcg acggcccccg cgtcggcgaa gtactgatca aatacaccgc cgcgggtttg tgccactcag acctgcacct gaccgacggc gacctgccac cgcggtatcc gattgtcggc ggccacgaag gatcgggcgt catcgaggaa gtcggtcccg gggtcagcaa ggtcaaaccc ggggaccacg tcgtgtgctc cttcatcccc aactgcggca cctgccgata ctgttccacc ggacggcaga acctttgcga catgggcgcg accatcctcg aaggatgcat gagcgatggc agcttccgct tccaccgcaa gggggtggac ttcggtgcga tgtgcatgct cggcaccttc tccgagcgcg ccaccatctc gcagcactcg gtggtcaaga tcgacgactg gttgccactg accaccgcgg tgctggtggg ctgcggagtt ccgaccggct ggggtacctc ggtatatgcc gggggcgtgc ggcccggcga cacgacggtc atctacggcg cgggcgggct gggcatcaac gccgtacaag gagcggtgag cgccggagcc aaacacgtcg tggtggtcga tccggttgcg ttcaagcggg acaccgcgct gaaattcggc gctacccatg ccttccccga cgcggccagc gcggccgcga angtcaacga aatcacctgg ggncnnngcg cggaccaagc gctcattctg gtcggcacgg tcganacgga agtggtcagt gccgcggctg cggtgatnnn caangncngn ccnnnnncat cancnggnnt ggnnaatccn nagaagctca cngngnaact gtcgnncnng gnncngancc tcaacnanaa gacnatcann ggnacactnn cgnatcgnnn antccccant acnannntcn nncgncnact cnncnntcna cgnnnnnncn nnaactcgac nnnctgatnn cnncccantn nnganannnn gtcaannnng nnnnntnnnn nnnnnnnnnn nnnnnnnnna nnnttnnnnn nnnnnncnnn cnnnnnnnnn nnngnnnnnn nnnnnnnnnn ncnnnnnnnn nnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTTAAVLL EAGKPFEIME LELDGPRVGE VLIKYTAAGL CHSDLHLTDG DLPPRYPIVG GHEGSGVIEE VGPGVSKVKP GDHVVCSFIP NCGTCRYCST GRQNLCDMGA TILEGCMSDG SFRFHRKGVD FGAMCMLGTF SERATISQHS VVKIDDWLPL TTAVLVGCGV PTGWGTSVYA GGVRPGDTTV IYGAGGLGIN AVQGAVSAGA KHVVVVDPVA FKRDTALKFG ATHAFPDAAS AAAKVNEITW GQGADQALIL VGTVDTEVVS AAAAVIGKGG TVVITGLANP EKLTVQLSGL DLTLNEKTIK GTLFGSANPQ YDIVRLLRLY DAGQLKLDEL ITTQYRLEDV NQGYQDLRDG KNIRGVIVHD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaccaca gcagccgtgt tgctggaagc gggcaagccc ttcgagatca tggaactcga actcgacggc ccccgcgtcg gcgaagtact gatcaaatac accgccgcgg gtttgtgcca ctcagacctg cacctgaccg acggcgacct gccaccgcgg tatccgattg tcggcggcca cgaaggatcg ggcgtcatcg aggaagtcgg tcccggggtc agcaaggtca aacccgggga ccacgtcgtg tgctccttca tccccaactg cggcacctgc cgatactgtt ccaccggacg gcagaacctt tgcgacatgg gcgcgaccat cctcgaagga tgcatgagcg atggcagctt ccgcttccac cgcaaggggg tggacttcgg tgcgatgtgc atgctcggca ccttctccga gcgcgccacc atctcgcagc actcggtggt caagatcgac gactggttgc cactgaccac cgcggtgctg gtgggctgcg gagttccgac cggctggggt acctcggtat atgccggggg cgtgcggccc ggcgacacga cggtcatcta cggcgcgggc gggctgggca tcaacgccgt acaaggagcg gtgagcgccg gagccaaaca cgtcgtggtg gtcgatccgg ttgcgttcaa gcgggacacc gcgctgaaat tcggcgctac ccatgccttc cccgacgcgg ccagcgcggc cgcgaaggtc aacgaaatca cctggggcca aggcgcggac caagcgctca ttctggtcgg cacggtcgac acggaagtgg tcagtgccgc ggctgcggtg atcggcaagg gcggcaccgt ggtcatcacc gggttggcga atccggagaa gctcacggtg caactgtcgg gcctggatct gaccctcaac gagaagacga tcaagggcac actgttcgga tcggccaatc cccaatacga catcgtgcgc ctactccggc tctacgacgc cggccaactc aaactcgacg agctgatcac cacccaatat cgactcgaag atgtcaacca gggctatcaa gatctgcgcg acggcaagaa cattcgcggc gtgatcgtcc acgacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa