MyabA.10611.c

Probable zinc-type alcohol dehydrogenase AdhD

CENTER ID: MyabA.10611.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.10611.c.A1.GE30548 Full length( MyabA.10611.c ) 1 368
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2122
RefSeq: YP_001702819.1
UniProt: B1MPB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTRAAVLWG LEQKWEVEEV DLDPPGPGEV LVRLAATGLC HSDEHLVTGD LPIPLPVVGG HEGAGTVVEC GEGVEDLSEG DSVILTFLPS CGRCSYCARG MGNLCELGAA LMMGPQIDGT YRFHARGEDV GQMCLLGTFS EYTVVPQASV VKIDGGIPLD RAALIGCGVT TGYGSAVRTG EVRAGDTVVV VGAGGIGMNA IQGARIAGAL AIVAVDPVNF KREQASGFGA THTAASMDEA WSLVSDMTRG KLADVCILTT DVAEGSYTAE ALALVGKRGR VVVTAIGHPD ESSISGSLLE MTLYEKQIRG ALYGSSNAPH DIPRLVELYS AGLLKLDELV TREYSLDQIN EGYQDMRSGR NIRGLVRF
NT Sequence
ATGAAGACAC GTGCGGCTGT TTTGTGGGGC TTGGAGCAGA AGTGGGAGGT CGAAGAAGTC GATTTGGATC CGCCAGGTCC CGGGGAGGTC CTCGTGCGGC TGGCGGCCAC CGGCTTGTGC CATTCCGACG AACATCTGGT GACCGGCGAC CTCCCGATCC CATTGCCGGT AGTGGGGGGT CATGAGGGTG CTGGCACAGT CGTGGAGTGC GGCGAAGGGG TCGAGGATCT GTCCGAAGGT GATTCTGTCA TCTTGACCTT CCTTCCCTCG TGTGGGCGCT GCTCGTACTG CGCGCGCGGC ATGGGCAATC TCTGTGAGTT GGGAGCGGCG CTCATGATGG GACCGCAGAT CGACGGCACC TATCGCTTTC ACGCACGAGG CGAGGACGTC GGGCAGATGT GTTTGCTCGG AACGTTTTCC GAGTACACAG TGGTGCCGCA GGCATCCGTG GTCAAGATTG ACGGCGGAAT CCCTCTGGAT CGGGCAGCCT TGATCGGGTG CGGCGTCACG ACCGGCTACG GATCTGCGGT GCGGACTGGG GAGGTGCGCG CCGGGGACAC CGTCGTGGTA GTGGGAGCAG GCGGAATCGG AATGAATGCG ATTCAGGGTG CGCGAATCGC GGGTGCGCTA GCAATTGTGG CGGTCGATCC GGTGAACTTC AAGCGAGAGC AAGCGAGCGG GTTCGGCGCG ACGCATACCG CCGCCTCGAT GGATGAAGCT TGGTCGCTGG TCAGCGATAT GACGCGGGGC AAGCTGGCCG ATGTCTGCAT CCTCACCACT GATGTAGCTG AGGGCTCTTA TACCGCCGAA GCGCTGGCCT TGGTCGGAAA GCGCGGACGC GTCGTGGTTA CGGCCATCGG GCATCCCGAC GAGTCGTCGA TCTCAGGGTC ACTGCTGGAG ATGACGCTTT ACGAGAAGCA GATTCGTGGC GCTTTGTATG GCTCATCCAA TGCGCCACAC GATATTCCGC GGCTGGTCGA GCTTTACTCC GCCGGGCTGT TGAAGCTCGA CGAGTTGGTC ACCCGCGAAT ACTCCCTCGA CCAAATCAAT GAGGGCTACC AGGATATGCG TTCAGGAAGA AACATTCGAG GGCTCGTCCG GTTC
Details for MyabA.10611.c.A1.GE30548
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKTRAAVLW GLEQKWEVEE VDLDPPGPGE VLVRLAATGL CHSDEHLVTG DLPIPLPVVG GHEGAGTVVE CGEGVEDLSE GDSVILTFLP SCGRCSYCAR GMGNLCELGA ALMMGPQIDG TYRFHARGED VGQMCLLGTF SEYTVVPQAS VVKIDGGIPL DRAALIGCGV TTGYGSAVRT GEVRAGDTVV VVGAGGIGMN AIXGARIAGA LAIVAVDPVN FKREQASGFG ATHTAASMDE AXSLVSDMTR GKLADVCILT TDVAXGSYTA EXLALVGKRG RVXVXXIGXP DESSISXHCX X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaga cacgtgcggc tgttttgtgg ggcttggagc agaagtggga ggtcgaagaa gtcgatttgg atccgccagg tcccggggag gtcctcgtgc ggctggcggc caccggcttg tgccattccg acgaacatct ggtgaccggc gacctcccga tcccattgcc ggtagtgggg ggtcatgagg gtgctggcac agtcgtggag tgcggcgaag gggtcgagga tctgtccgaa ggtgattctg tcatcttgac cttccttccc tcgtgtgggc gctgctcgta ctgcgcgcgc ggcatgggca atctctgtga gttgggagcg gcgctcatga tgggaccgca gatcgacggc acctatcgct ttcacgcacg aggcgaggac gtcgggcaga tgtgtttgct cggaacgttt tccgagtaca cagtggtgcc gcaggcatcc gtggtcaaga ttgacggcgg aatccctctg gatcgggcag ccttgatcgg gtgcggcgtc acgaccggct acggatctgc ggtgcggact ggggaggtgc gcgccgggga caccgtcgtg gtagtgggag caggcggaat cggaatgaat gcgattcang gtgcgcgaat cgcgggtgcg ctagcaattg tggcggtcga tccggtgaac ttcaagcgag agcaagcgag cgggttcggc gcgacgcata ccgccgcctc gatggatgaa gcttgntcgc tggtcagcga tatgacgcgg ggcaagctgg ccgatgtctg catcctcacc actgatgtag ctganggctc ttataccgcc gaancgctgg ccttggtcgg aaagcgcggn cgcgtcgngg ttannnccat cgggcntccc gacgagtcgt cnatctcnnn ncactgctnn nnatgacgct ttacganaag canatncnng gcnctttngn ggctcatnnn tgcncnacnc gatannccnc nnnggnngag cnttnnncnn nggcnnnnna gctcgannnn nnnnnacccn nnnnnnnnnc nnanntannn nnnnnnannn na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTRAAVLW GLEQKWEVEE VDLDPPGPGE VLVRLAATGL CHSDEHLVTG DLPIPLPVVG GHEGAGTVVE CGEGVEDLSE GDSVILTFLP SCGRCSYCAR GMGNLCELGA ALMMGPQIDG TYRFHARGED VGQMCLLGTF SEYTVVPQAS VVKIDGGIPL DRAALIGCGV TTGYGSAVRT GEVRAGDTVV VVGAGGIGMN AIQGARIAGA LAIVAVDPVN FKREQASGFG ATHTAASMDE AWSLVSDMTR GKLADVCILT TDVAEGSYTA EALALVGKRG RVVVTAIGHP DESSISGSLL EMTLYEKQIR GALYGSSNAP HDIPRLVELY SAGLLKLDEL VTREYSLDQI NEGYQDMRSG RNIRGLVRF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagacacgt gcggctgttt tgtggggctt ggagcagaag tgggaggtcg aagaagtcga tttggatccg ccaggtcccg gggaggtcct cgtgcggctg gcggccaccg gcttgtgcca ttccgacgaa catctggtga ccggcgacct cccgatccca ttgccggtag tggggggtca tgagggtgct ggcacagtcg tggagtgcgg cgaaggggtc gaggatctgt ccgaaggtga ttctgtcatc ttgaccttcc ttccctcgtg tgggcgctgc tcgtactgcg cgcgcggcat gggcaatctc tgtgagttgg gagcggcgct catgatggga ccgcagatcg acggcaccta tcgctttcac gcacgaggcg aggacgtcgg gcagatgtgt ttgctcggaa cgttttccga gtacacagtg gtgccgcagg catccgtggt caagattgac ggcggaatcc ctctggatcg ggcagccttg atcgggtgcg gcgtcacgac cggctacgga tctgcggtgc ggactgggga ggtgcgcgcc ggggacaccg tcgtggtagt gggagcaggc ggaatcggaa tgaatgcgat tcagggtgcg cgaatcgcgg gtgcgctagc aattgtggcg gtcgatccgg tgaacttcaa gcgagagcaa gcgagcgggt tcggcgcgac gcataccgcc gcctcgatgg atgaagcttg gtcgctggtc agcgatatga cgcggggcaa gctggccgat gtctgcatcc tcaccactga tgtagctgag ggctcttata ccgccgaagc gctggccttg gtcggaaagc gcggacgcgt cgtggttacg gccatcgggc atcccgacga gtcgtcgatc tcagggtcac tgctggagat gacgctttac gagaagcaga ttcgtggcgc tttgtatggc tcatccaatg cgccacacga tattccgcgg ctggtcgagc tttactccgc cgggctgttg aagctcgacg agttggtcac ccgcgaatac tccctcgacc aaatcaatga gggctaccag gatatgcgtt caggaagaaa cattcgaggg ctcgtccggt tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa