MymaA.10611.d

Zinc-type alcohol dehydrogenase AdhD

CENTER ID: MymaA.10611.d
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.10611.d.A1.GE30239 Full length( MymaA.10611.d ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.278
RefSeq: YP_001848565.1
UniProt: B2HKK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSRAAILYE YGHPWTVEEF ELDPPRAGEV LVQLSATGLC HSDEHIRRGN LAPPPETLRS LGLPAMSPTI GGHEGSGVVL EVGEGVTRFA PGDHVVTSFI AVCGQCRWCA TGMEYLCDTG YGTLAPGMPT DGTFRHHSLD GRDLGHVSKI GAFAEHTVVS ADSLVKIDQT FPLVPAALLA CAIPTGYGSA ARRAQVRGGD IAVVIGAGGI GTAAIQGARI QGATRIIAVD PVEFKRKSAL TFGATHTAAT TDEAVETVRE LTRGVMADVV VVSPATIGPA DVAAALSLTR KGGTCVLTGL PAPAMHSIAV GLQDFVLMNK TLCGTVFGSC NPKSDIPLLA TMYQSGQLLL DEMITKRYSL DSINDAYADL VAGELIRGVI DFSLS
NT Sequence
ATGAGAAGCC GCGCGGCCAT CCTTTATGAG TACGGTCACC CCTGGACCGT CGAGGAATTC GAGCTCGACC CGCCGCGCGC CGGCGAGGTG CTGGTGCAAC TGAGCGCGAC CGGACTGTGC CACTCCGATG AACACATCCG ACGGGGCAAC CTCGCGCCAC CACCGGAAAC ACTGCGCTCT TTGGGGCTGC CGGCCATGTC CCCGACTATC GGCGGCCATG AAGGATCCGG CGTCGTGCTC GAGGTCGGCG AGGGGGTGAC CCGGTTCGCA CCCGGCGACC ATGTGGTGAC GTCTTTCATC GCGGTGTGCG GCCAGTGCCG TTGGTGTGCA ACGGGAATGG AATACCTGTG CGATACCGGA TACGGAACCT TGGCACCCGG AATGCCCACC GACGGTACCT TCCGTCATCA CAGCCTCGAT GGACGCGACC TCGGCCACGT GTCCAAGATC GGCGCGTTCG CCGAACATAC GGTGGTCTCG GCCGATTCGC TGGTCAAGAT TGATCAGACG TTCCCGCTGG TGCCGGCCGC CTTGCTGGCC TGTGCGATAC CGACCGGATA TGGCTCGGCG GCACGACGCG CTCAGGTCCG CGGCGGCGAC ATCGCCGTGG TGATCGGCGC GGGCGGCATC GGAACAGCGG CCATCCAAGG CGCCCGTATC CAGGGAGCGA CCCGCATCAT CGCGGTCGAT CCCGTTGAGT TCAAGCGCAA ATCCGCGCTG ACCTTTGGGG CCACTCATAC CGCGGCGACC ACGGACGAGG CCGTTGAGAC GGTGCGCGAG CTAACTCGCG GCGTGATGGC GGATGTGGTC GTGGTATCCC CCGCCACGAT CGGCCCGGCC GACGTCGCGG CGGCACTGTC GCTGACCCGC AAGGGCGGAA CCTGCGTGCT CACCGGTCTG CCGGCCCCCG CGATGCACTC CATCGCCGTC GGTCTTCAAG ACTTCGTGCT GATGAATAAG ACGCTGTGTG GCACGGTCTT TGGCTCGTGC AACCCAAAGA GTGACATTCC GCTGCTGGCC ACGATGTACC AGTCGGGGCA GTTACTCTTG GACGAGATGA TCACCAAGCG CTACTCGCTC GACAGCATCA ACGACGCCTA CGCCGATCTG GTTGCCGGCG AGTTGATCCG CGGGGTGATC GATTTCAGTC TGAGC
Details for MymaA.10611.d.A1.GE30239
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRSRAAILY EYGHPWTVEE FELDPPRAGE VLVQLSATGL CHSDEHIRRG NLAPPPETLR SLGLPAMSPT IGGHEGSGVV LEVGEGVTRF APGDHVVTSF IAVCGQCRWC ATGMEYLCDT GYGTLAPGMP TDGTFRHHSL DGRDLGHVSK IGAFAEHTVV SADSLVKIDQ TFPLVPAALL ACAIPTGYGS AARRAQVRGG DIAVVIGAGG IGTAAIQGAR IQGATRIIAV DPVEFKRKSA LTFGATHTAA TTDXAVEXVR ELTRGVMADV VVVSPAXIGP GRXRGGTVAD XXXGXCVLXV XXPXMXXSXX XXXX
Validated NT Sequence
ttgttnnnnt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgagaagcc gcgcggccat cctttatgag tacggtcacc cctggaccgt cgaggaattc gagctcgacc cgccgcgcgc cggcgaggtg ctggtgcaac tgagcgcgac cggactgtgc cactccgatg aacacatccg acggggcaac ctcgcgccac caccggaaac actgcgctct ttggggctgc cggccatgtc cccgactatc ggcggccatg aaggatccgg cgtcgtgctc gaggtcggcg agggggtgac ccggttcgca cccggcgacc atgtggtgac gtctttcatc gcggtgtgcg gccagtgccg ttggtgtgca acgggaatgg aatacctgtg cgataccgga tacggaacct tggcacccgg aatgcccacc gacggtacct tccgtcatca cagcctcgat ggacgcgacc tcggccacgt gtccaagatc ggcgcgttcg ccgaacatac ggtggtctcg gccgattcgc tggtcaagat tgatcagacg ttcccgctgg tgccggccgc cttgctggcc tgtgcgatac cgaccggata tggctcggcg gcacgacgcg ctcaggtccg cggcggcgac atcgccgtgg tgatcggcgc gggcggcatc ggaacagcgg ccatccaagg cgcccgtatc cagggagcga cccgcatcat cgcggtcgat cccgttgagt tcaagcgcaa atccgcgctg acctttgggg ccactcatac cgcggcgacc acggacgang ccgttgagan ngtgcgcgag ctnactcgcg gcgtgatggc ggatgtggtc gtggtatccc ccgcnncgat cgggccnggc cgangtcgcg gcggnactgt cgctgaccnn cnngnnggaa nntgcgtgct cncngtctgn nnncccnnga tgcnnncntc nnnnnnnnnn anantnnnct gatgatanan gctnngnngn nngnnnttgn ncnngnancn nannnnnnnn ctnnnncnnn ntncnntnnn nnnnnnnnnn ntnnnnnnan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn annnannnnn nnnnnnnnnn cnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRSRAAILY EYGHPWTVEE FELDPPRAGE VLVQLSATGL CHSDEHIRRG NLAPPPETLR SLGLPAMSPT IGGHEGSGVV LEVGEGVTRF APGDHVVTSF IAVCGQCRWC ATGMEYLCDT GYGTLAPGMP TDGTFRHHSL DGRDLGHVSK IGAFAEHTVV SADSLVKIDQ TFPLVPAALL ACAIPTGYGS AARRAQVRGG DIAVVIGAGG IGTAAIQGAR IQGATRIIAV DPVEFKRKSA LTFGATHTAA TTDEAVETVR ELTRGVMADV VVVSPATIGP ADVAAALSLT RKGGTCVLTG LPAPAMHSIA VGLQDFVLMN KTLCGTVFGS CNPKSDIPLL ATMYQSGQLL LDEMITKRYS LDSINDAYAD LVAGELIRGV IDFSLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagaagccgc gcggccatcc tttatgagta cggtcacccc tggaccgtcg aggaattcga gctcgacccg ccgcgcgccg gcgaggtgct ggtgcaactg agcgcgaccg gactgtgcca ctccgatgaa cacatccgac ggggcaacct cgcgccacca ccggaaacac tgcgctcttt ggggctgccg gccatgtccc cgactatcgg cggccatgaa ggatccggcg tcgtgctcga ggtcggcgag ggggtgaccc ggttcgcacc cggcgaccat gtggtgacgt ctttcatcgc ggtgtgcggc cagtgccgtt ggtgtgcaac gggaatggaa tacctgtgcg ataccggata cggaaccttg gcacccggaa tgcccaccga cggtaccttc cgtcatcaca gcctcgatgg acgcgacctc ggccacgtgt ccaagatcgg cgcgttcgcc gaacatacgg tggtctcggc cgattcgctg gtcaagattg atcagacgtt cccgctggtg ccggccgcct tgctggcctg tgcgataccg accggatatg gctcggcggc acgacgcgct caggtccgcg gcggcgacat cgccgtggtg atcggcgcgg gcggcatcgg aacagcggcc atccaaggcg cccgtatcca gggagcgacc cgcatcatcg cggtcgatcc cgttgagttc aagcgcaaat ccgcgctgac ctttggggcc actcataccg cggcgaccac ggacgaggcc gttgagacgg tgcgcgagct aactcgcggc gtgatggcgg atgtggtcgt ggtatccccc gccacgatcg gcccggccga cgtcgcggcg gcactgtcgc tgacccgcaa gggcggaacc tgcgtgctca ccggtctgcc ggcccccgcg atgcactcca tcgccgtcgg tcttcaagac ttcgtgctga tgaataagac gctgtgtggc acggtctttg gctcgtgcaa cccaaagagt gacattccgc tgctggccac gatgtaccag tcggggcagt tactcttgga cgagatgatc accaagcgct actcgctcga cagcatcaac gacgcctacg ccgatctggt tgccggcgag ttgatccgcg gggtgatcga tttcagtctg agcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa