MytuD.01625.a
S-adenosylmethionine synthetase
| CENTER ID: | MytuD.01625.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01625.a.A1.GE28467 | Full length( MytuD.01625.a ) | 1 | 403 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3TDE
DEPOSITED: 8/10/2011
DETERMINATION: XRay
CLONE: MytuD.01625.a.A1.GE28467
PROTEIN: MytuD.01625.a.A1.PS00654
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|83332.12.peg.1557 |
| RefSeq: | NP_215908.1 |
| TubercuList: | Rv1392 |
| MTB Network Portal: | Rv1392 |
| UniProt: | P9WGV1 |
Orthologues for Rv1392
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEKGRLFTS ESVTEGHPDK ICDAISDSVL DALLAADPRS RVAVETLVTT GQVHVVGEVT TSAKEAFADI TNTVRARILE IGYDSSDKGF DGATCGVNIG IGAQSPDIAQ GVDTAHEARV EGAADPLDSQ GAGDQGLMFG YAINATPELM PLPIALAHRL SRRLTEVRKN GVLPYLRPDG KTQVTIAYED NVPVRLDTVV ISTQHAADID LEKTLDPDIR EKVLNTVLDD LAHETLDAST VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR WVAKNVVAAG LAERVEVQVA YAIGKAAPVG LFVETFGTET EDPVKIEKAI GEVFDLRPGA IIRDLNLLRP IYAPTAAYGH FGRTDVELPW EQLDKVDDLK RAI
NT Sequence
GTGAGCGAAA AGGGTCGGCT GTTTACCAGT GAGTCGGTGA CAGAGGGACA TCCCGACAAG ATCTGTGACG CCATCAGCGA CTCGGTTCTG GACGCGCTTC TAGCGGCGGA CCCGCGCTCA CGTGTCGCGG TCGAGACGCT GGTGACCACC GGGCAGGTGC ACGTGGTGGG TGAGGTGACC ACCTCGGCTA AGGAGGCGTT TGCCGACATC ACCAACACGG TCCGCGCACG GATCCTCGAG ATCGGCTACG ACTCGTCGGA CAAGGGTTTC GACGGGGCGA CCTGCGGGGT GAACATCGGC ATCGGCGCAC AGTCACCCGA CATCGCCCAG GGGGTCGACA CCGCCCACGA GGCCCGGGTC GAGGGCGCGG CCGATCCGCT GGACTCCCAG GGCGCCGGTG ACCAGGGCCT GATGTTCGGC TACGCGATCA ATGCCACCCC GGAACTGATG CCACTGCCCA TCGCGCTGGC CCACCGACTG TCGCGGCGGC TGACCGAGGT CCGCAAGAAC GGGGTGCTGC CCTACCTGCG TCCGGATGGC AAGACGCAGG TCACTATCGC CTACGAGGAC AACGTTCCGG TGCGGCTGGA TACCGTGGTC ATCTCCACCC AGCACGCGGC CGATATCGAC CTGGAGAAGA CGCTTGATCC CGACATCCGG GAAAAGGTGC TCAACACCGT GCTCGACGAC CTGGCCCACG AAACCCTGGA CGCGTCGACG GTGCGGGTGC TGGTGAACCC GACCGGCAAG TTCGTGCTCG GCGGGCCGAT GGGCGATGCC GGGCTCACCG GCCGCAAGAT CATCGTCGAC ACCTACGGCG GCTGGGCCCG CCACGGCGGC GGCGCCTTCT CCGGCAAGGA TCCGTCCAAG GTGGACCGGT CGGCGGCGTA CGCGATGCGC TGGGTGGCCA AGAATGTCGT CGCCGCCGGG TTGGCTGAAC GGGTCGAGGT GCAGGTGGCC TACGCCATCG GTAAAGCGGC ACCCGTCGGC CTGTTCGTCG AGACGTTCGG TACCGAGACG GAAGACCCGG TCAAGATCGA GAAGGCCATC GGCGAGGTAT TCGACCTGCG CCCCGGTGCC ATCATCCGCG ACCTGAACCT GTTGCGCCCG ATCTATGCGC CGACCGCCGC CTACGGGCAC TTCGGCCGCA CCGACGTCGA ATTACCGTGG GAGCAGCTCG ACAAGGTCGA CGACCTCAAG CGCGCCATC
Details for MytuD.01625.a.A1.GE28467
| HARVESTED ON: | 2/2/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEKGRLF TSESVTEGHP DKICDAISDS VLDALLAADP RSRVAVETLV TTGQVHVVGE VTTSAKEAFA DITNTVRARI LEIGYDSSDK GFDGATCGVN IGIGAQSPDI AQGVDTAHEA RVEGAADPLD SQGAGDQGLM FGYAINATPE LMPLPIALAH RLSRRLTEVR KNGVLPYLRP DGKTQVTIAY EDNVPVRLDT VVISTQHAAD IDLEKTLDPD IREKVLNTVL DDLAHETLDA STVRVLVNPT GKFVLGGPMG DAGLTGRKII VDTYGGWARH GGGAFSGKDP SKVDRSAAYA MRWVAKNVVA AGLAERVEVQ VAYAIGKAAP VGLFVETFGT ETEDPVKIEK AIGEVFDLRP GAIIRDLNLL RPIYAPTAAY GHFGRTDVEL PWEQLDKVDD LKRAI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcgaaaaggg tcggctgttt accagtgagt cggtgacaga gggacatccc gacaagatct gtgacgccat cagcgactcg gttctggacg cgcttctagc ggcggacccg cgctcacgtg tcgcggtcga gacgctggtg accaccgggc aggtgcacgt ggtgggtgag gtgaccacct cggctaagga ggcgtttgcc gacatcacca acacggtccg cgcacggatc ctcgagatcg gctacgactc gtcggacaag ggtttcgacg gggcgacctg cggggtgaac atcggcatcg gcgcacagtc acccgacatc gcccaggggg tcgacaccgc ccacgaggcc cgggtcgagg gcgcggccga tccgctggac tcccagggcg ccggtgacca gggcctgatg ttcggctacg cgatcaatgc caccccggaa ctgatgccac tgcccatcgc gctggcccac cgactgtcgc ggcggctgac cgaggtccgc aagaacgggg tgctgcccta cctgcgtccg gatggcaaga cgcaggtcac tatcgcctac gaggacaacg ttccggtgcg gctggatacc gtggtcatct ccacccagca cgcggccgat atcgacctgg agaagacgct tgatcccgac atccgggaaa aggtgctcaa caccgtgctc gacgacctgg cccacgaaac cctggacgcg tcgacggtgc gggtgctggt gaacccgacc ggcaagttcg tgctcggcgg gccgatgggc gatgccgggc tcaccggccg caagatcatc gtcgacacct acggcggctg ggcccgccac ggcggcggcg ccttctccgg caaggatccg tccaaggtgg accggtcggc ggcgtacgcg atgcgctggg tggccaagaa tgtcgtcgcc gccgggttgg ctgaacgggt cgaggtgcag gtggcctacg ccatcggtaa agcggcaccc gtcggcctgt tcgtcgagac gttcggtacc gagacggaag acccggtcaa gatcgagaag gccatcggcg aggtattcga cctgcgcccc ggtgccatca tccgcgacct gaacctgttg cgcccgatct atgcgccgac cgccgcctac gggcacttcg gccgcaccga cgtcgaatta ccgtgggagc agctcgacaa ggtcgacgac ctcaagcgcg ccatctaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEKGRLF TSESVTEGHP DKICDAISDS VLDALLAADP RSRVAVETLV TTGQVHVVGE VTTSAKEAFA DITNTVRARI LEIGYDSSDK GFDGATCGVN IGIGAQSPDI AQGVDTAHEA RVEGAADPLD SQGAGDQGLM FGYAINATPE LMPLPIALAH RLSRRLTEVR KNGVLPYLRP DGKTQVTIAY EDNVPVRLDT VVISTQHAAD IDLEKTLDPD IREKVLNTVL DDLAHETLDA STVRVLVNPT GKFVLGGPMG DAGLTGRKII VDTYGGWARH GGGAFSGKDP SKVDRSAAYA MRWVAKNVVA AGLAERVEVQ VAYAIGKAAP VGLFVETFGT ETEDPVKIEK AIGEVFDLRP GAIIRDLNLL RPIYAPTAAY GHFGRTDVEL PWEQLDKVDD LKRAI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgaa aagggtcggc tgtttaccag tgagtcggtg acagagggac atcccgacaa gatctgtgac gccatcagcg actcggttct ggacgcgctt ctagcggcgg acccgcgctc acgtgtcgcg gtcgagacgc tggtgaccac cgggcaggtg cacgtggtgg gtgaggtgac cacctcggct aaggaggcgt ttgccgacat caccaacacg gtccgcgcac ggatcctcga gatcggctac gactcgtcgg acaagggttt cgacggggcg acctgcgggg tgaacatcgg catcggcgca cagtcacccg acatcgccca gggggtcgac accgcccacg aggcccgggt cgagggcgcg gccgatccgc tggactccca gggcgccggt gaccagggcc tgatgttcgg ctacgcgatc aatgccaccc cggaactgat gccactgccc atcgcgctgg cccaccgact gtcgcggcgg ctgaccgagg tccgcaagaa cggggtgctg ccctacctgc gtccggatgg caagacgcag gtcactatcg cctacgagga caacgttccg gtgcggctgg ataccgtggt catctccacc cagcacgcgg ccgatatcga cctggagaag acgcttgatc ccgacatccg ggaaaaggtg ctcaacaccg tgctcgacga cctggcccac gaaaccctgg acgcgtcgac ggtgcgggtg ctggtgaacc cgaccggcaa gttcgtgctc ggcgggccga tgggcgatgc cgggctcacc ggccgcaaga tcatcgtcga cacctacggc ggctgggccc gccacggcgg cggcgccttc tccggcaagg atccgtccaa ggtggaccgg tcggcggcgt acgcgatgcg ctgggtggcc aagaatgtcg tcgccgccgg gttggctgaa cgggtcgagg tgcaggtggc ctacgccatc ggtaaagcgg cacccgtcgg cctgttcgtc gagacgttcg gtaccgagac ggaagacccg gtcaagatcg agaaggccat cggcgaggta ttcgacctgc gccccggtgc catcatccgc gacctgaacc tgttgcgccc gatctatgcg ccgaccgccg cctacgggca cttcggccgc accgacgtcg aattaccgtg ggagcagctc gacaaggtcg acgacctcaa gcgcgccatc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa