MyabA.01625.a

S-adenosylmethionine synthetase

CENTER ID: MyabA.01625.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01625.a.A1.GE30535 Full length( MyabA.01625.a ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2867
RefSeq: YP_001703553.1
UniProt: B1MCC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEGHPDKIC DAISDSILDA MLAQDPKSRV AVETLVTTGQ VHVAGEVTTS AYVDIPHIVR ERVLEIGYDS SSKGFDGNSC GVNVAIGAQS PDIAQGVDTA HEARVEGAAD PLDAQGAGDQ GLMFGYAIND TPELMPLPIA LAHRLSRRLT EVRKDGLLPY LRPDGKTQVT IEYDGNKAVR LDTVVLSTQH AADIDLENLL TPDIREKVVQ VVLDELNQDG LDISDYRLLV NPTGKFVLGG PMGDAGLTGR KIIVDTYGGW ARHGGGAFSG KDPSKVDRSA AYAMRWVAKN VVAAGLADRA EVQVAYAIGK AAPVGLFVDT FGTATVDQAK IEKAINEVFD LRPGAIIRDL DLLRPIYAQT AAYGHFGRTD VELPWEQTDR VDALKAAI
NT Sequence
GTGACCGAAG GGCATCCGGA CAAGATCTGT GACGCCATCA GTGACTCGAT CCTGGACGCC ATGCTCGCGC AGGACCCGAA GTCCCGGGTG GCGGTGGAGA CGCTGGTGAC CACCGGTCAG GTCCATGTCG CCGGCGAGGT GACTACCTCC GCCTATGTCG ATATCCCGCA CATCGTCCGG GAAAGAGTGC TGGAAATCGG CTATGACTCC TCTTCCAAGG GCTTCGATGG AAACTCCTGT GGTGTGAATG TCGCCATCGG TGCGCAGTCA CCCGATATCG CGCAAGGTGT CGACACGGCC CACGAGGCCC GCGTTGAGGG CGCCGCGGAT CCGCTGGACG CTCAGGGAGC CGGCGATCAG GGCCTGATGT TCGGCTACGC CATCAACGAC ACCCCCGAGC TGATGCCGCT GCCCATCGCG CTGGCACACC GGCTCTCGCG CCGCCTGACT GAGGTGCGCA AGGATGGGCT GCTGCCGTAC CTGCGTCCCG ACGGTAAGAC TCAGGTCACC ATCGAGTATG ACGGCAACAA GGCCGTCCGG CTGGACACTG TCGTGCTCTC CACGCAGCAC GCCGCGGATA TCGATCTCGA AAATCTGCTG ACCCCGGACA TCCGCGAGAA GGTGGTCCAG GTAGTGCTCG ACGAGCTGAA CCAGGATGGT CTGGACATCT CCGACTACCG GCTGCTGGTC AACCCGACGG GCAAGTTCGT GCTCGGCGGG CCGATGGGTG ACGCCGGACT GACCGGCCGC AAGATCATCG TCGACACCTA CGGCGGCTGG GCCCGCCACG GCGGCGGCGC GTTCTCGGGC AAGGACCCGT CAAAGGTGGA CCGTTCCGCC GCGTACGCGA TGCGCTGGGT GGCCAAGAAT GTCGTCGCGG CCGGCCTCGC CGACCGGGCC GAGGTGCAGG TCGCGTACGC CATCGGCAAG GCAGCGCCGG TCGGTCTGTT CGTTGACACG TTCGGTACCG CCACCGTCGA TCAGGCCAAG ATTGAGAAGG CCATCAACGA GGTGTTCGAC CTGCGTCCCG GCGCCATCAT CCGCGACCTG GACCTCCTGC GTCCCATCTA CGCGCAGACC GCCGCGTACG GCCACTTCGG CCGCACCGAT GTCGAGCTGC CCTGGGAACA GACCGACCGC GTCGATGCGC TCAAGGCCGC CATT
Details for MyabA.01625.a.A1.GE30535
HARVESTED ON: 9/21/2010
SEQUENCED ON: 4/19/2017
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 73
Validated AA Sequence
MGTLEAQTQG PGSMVTEGHP DKICDAISDS ILDAMLAQDP KSRVAVETLV TTGQVHVAGE VTTSAYVDIP HIVRERVLEI GYDSSSKGFD GNSCGVNVAI GAQSPDIAQG VDTAHEARVE GAADPLDAQG AGDQGLMFGY AINDTPELMP LPIALAHRLS RRLTEVRKDG LLPYLRPDGK TQVTIEYDGN KAVRLDTVVL STQHAADIDL ENLLTPDIRE KVVQVVLDEL NQDGLDISDY RLLVNPTGKF VLGGPMGDAG LTGRKIIVDT YGGWARHGGG AFSGKXPSXV DRSAAYAMRW V
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgacc gaagggcatc cggacaagat ctgtgacgcc atcagtgact cgatcctgga cgccatgctc gcgcaggacc cgaagtcccg ggtggcggtg gagacgctgg tgaccaccgg tcaggtccat gtcgccggcg aggtgactac ctccgcctat gtcgatatcc cgcacatcgt ccgggaaaga gtgctggaaa tcggctatga ctcctcttcc aagggcttcg atggaaactc ctgtggtgtg aatgtcgcca tcggtgcgca gtcacccgat atcgcgcaag gtgtcgacac ggcccacgag gcccgcgttg agggcgccgc ggatccgctg gacgctcagg gagccggcga tcagggcctg atgttcggct acgccatcaa cgacaccccc gagctgatgc cgctgcccat cgcgctggca caccggctct cgcgccgcct gactgaggtg cgcaaggatg ggctgctgcc gtacctgcgt cccgacggta agactcaggt caccatcgag tatgacggca acaaggccgt ccggctggac actgtcgtgc tctccacgca gcacgccgcg gatatcgatc tcgaaaatct gctgaccccg gacatccgcg agaaggtggt ccaggtagtg ctcgacgagc tgaaccagga tggtctggac atctccgact accggctgct ggtcaacccg acgggcaagt tcgtgctcgg cgggccgatg ggtgacgccg gactgaccgg ccgcaagatc atcgtcgaca cctacggcgg ctgggcccgc cacggcggcg gcgcgttctc gggcaaggan ccgtcaaang tggaccgttc cgccgcgtac gcnatgcgct gggtgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTEGHPDK ICDAISDSIL DAMLAQDPKS RVAVETLVTT GQVHVAGEVT TSAYVDIPHI VRERVLEIGY DSSSKGFDGN SCGVNVAIGA QSPDIAQGVD TAHEARVEGA ADPLDAQGAG DQGLMFGYAI NDTPELMPLP IALAHRLSRR LTEVRKDGLL PYLRPDGKTQ VTIEYDGNKA VRLDTVVLST QHAADIDLEN LLTPDIREKV VQVVLDELNQ DGLDISDYRL LVNPTGKFVL GGPMGDAGLT GRKIIVDTYG GWARHGGGAF SGKDPSKVDR SAAYAMRWVA KNVVAAGLAD RAEVQVAYAI GKAAPVGLFV DTFGTATVDQ AKIEKAINEV FDLRPGAIIR DLDLLRPIYA QTAAYGHFGR TDVELPWEQT DRVDALKAAI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgaa gggcatccgg acaagatctg tgacgccatc agtgactcga tcctggacgc catgctcgcg caggacccga agtcccgggt ggcggtggag acgctggtga ccaccggtca ggtccatgtc gccggcgagg tgactacctc cgcctatgtc gatatcccgc acatcgtccg ggaaagagtg ctggaaatcg gctatgactc ctcttccaag ggcttcgatg gaaactcctg tggtgtgaat gtcgccatcg gtgcgcagtc acccgatatc gcgcaaggtg tcgacacggc ccacgaggcc cgcgttgagg gcgccgcgga tccgctggac gctcagggag ccggcgatca gggcctgatg ttcggctacg ccatcaacga cacccccgag ctgatgccgc tgcccatcgc gctggcacac cggctctcgc gccgcctgac tgaggtgcgc aaggatgggc tgctgccgta cctgcgtccc gacggtaaga ctcaggtcac catcgagtat gacggcaaca aggccgtccg gctggacact gtcgtgctct ccacgcagca cgccgcggat atcgatctcg aaaatctgct gaccccggac atccgcgaga aggtggtcca ggtagtgctc gacgagctga accaggatgg tctggacatc tccgactacc ggctgctggt caacccgacg ggcaagttcg tgctcggcgg gccgatgggt gacgccggac tgaccggccg caagatcatc gtcgacacct acggcggctg ggcccgccac ggcggcggcg cgttctcggg caaggacccg tcaaaggtgg accgttccgc cgcgtacgcg atgcgctggg tggccaagaa tgtcgtcgcg gccggcctcg ccgaccgggc cgaggtgcag gtcgcgtacg ccatcggcaa ggcagcgccg gtcggtctgt tcgttgacac gttcggtacc gccaccgtcg atcaggccaa gattgagaag gccatcaacg aggtgttcga cctgcgtccc ggcgccatca tccgcgacct ggacctcctg cgtcccatct acgcgcagac cgccgcgtac ggccacttcg gccgcaccga tgtcgagctg ccctgggaac agaccgaccg cgtcgatgcg ctcaaggccg ccatttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa