MysmA.01625.a

S-adenosylmethionine synthetase

CENTER ID: MysmA.01625.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01625.a.A1.PW28876 Full length( MysmA.01625.a ) 1 399

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3016
RefSeq: YP_887371.1
UniProt: A0QWT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKGRLFTSE SVTEGHPDKI CDAISDSVLD ALLEQDPKSR VAVETLVTTG QVHVAGEVTT TAYADIPKIV RDRILDIGYD SSTKGFDGAS CGVNVAIGAQ SPDIAQGVDT AHETRVEGKA DPLDLQGAGD QGLMFGYAIG DTPELMPLPI ALAHRLARRL TEVRKNGVLD YLRPDGKTQV TIQYDGTTPV RLDTVVLSTQ HADGIDLEGT LTPDIREKVV NTVLADLGHE TLDTSDYRLL VNPTGKFVLG GPMGDAGLTG RKIIVDTYGG WARHGGGAFS GKDPSKVDRS AAYAMRWVAK NVVAAGLAER VEVQVAYAIG KAAPVGLFVE TFGSETVDPA KIEKAIGEVF DLRPAAIVRD LDLLRPIYAP TAAYGHFGRT DIELPWEQTN KVDDLKSAI
NT Sequence
GTGAGCAAAG GTCGCCTGTT TACCAGTGAG TCGGTAACCG AAGGGCACCC CGACAAGATC TGTGACGCAA TCAGCGACTC TGTGCTCGAC GCGCTGCTGG AGCAGGATCC CAAGTCCCGC GTCGCCGTCG AGACGCTGGT CACCACGGGC CAGGTGCACG TCGCCGGTGA GGTGACCACC ACGGCGTACG CCGACATCCC CAAGATCGTG CGCGACCGCA TCCTCGACAT CGGCTACGAC TCGTCGACCA AGGGCTTCGA CGGCGCCTCG TGCGGCGTCA ACGTGGCGAT CGGTGCGCAG TCGCCCGACA TCGCCCAAGG CGTCGACACC GCCCACGAGA CCCGCGTCGA GGGCAAGGCC GACCCGCTGG ACCTGCAGGG CGCAGGCGAC CAGGGCCTGA TGTTCGGCTA CGCCATCGGC GACACCCCCG AACTCATGCC GCTGCCCATC GCGCTGGCCC ACCGCCTCGC GCGCCGCCTG ACCGAGGTGC GCAAGAACGG TGTGCTGGAC TACCTGCGGC CCGACGGCAA GACCCAGGTC ACCATCCAGT ACGACGGCAC CACCCCGGTG CGGTTGGACA CCGTGGTGCT GTCCACCCAG CACGCCGACG GCATCGACCT GGAGGGCACC CTCACGCCCG ACATCCGCGA GAAGGTCGTC AACACCGTGC TCGCCGATCT CGGCCATGAG ACCCTCGACA CGTCCGACTA CCGCCTGCTG GTCAACCCGA CGGGCAAGTT CGTCCTCGGC GGCCCCATGG GCGACGCCGG CCTGACCGGC CGCAAGATCA TCGTCGACAC CTACGGCGGC TGGGCTCGCC ACGGCGGCGG CGCCTTCTCG GGCAAGGATC CGTCAAAGGT CGACCGCTCG GCCGCCTACG CGATGCGCTG GGTCGCCAAG AACGTCGTCG CCGCTGGCCT GGCCGAGCGC GTCGAGGTGC AGGTCGCCTA CGCGATCGGC AAGGCCGCCC CGGTGGGTCT GTTCGTGGAG ACCTTCGGCA GCGAGACCGT CGACCCGGCC AAGATCGAGA AGGCCATCGG CGAGGTGTTC GACCTGCGTC CCGCCGCGAT CGTGCGCGAC CTCGACCTGC TGCGCCCGAT CTACGCGCCC ACCGCCGCGT ACGGACACTT CGGCCGCACC GACATCGAGC TGCCGTGGGA GCAGACCAAC AAGGTGGACG ACCTGAAGTC CGCCATC
Details for MysmA.01625.a.A1.PW28876
PURIFICATION DATe: 3/25/2011
CONCENTRATION: 36.98mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 34
Protocol Notes
notes unavailable
Validated AA Sequence
MGDAGLTGRK IIVDTYGGWA RHGGGAFSGK DPSKVDRSAA YAMRWVAKNV VAAGLAERVE VQVAYAIGKA APVGLFVETF GSETVDPAKI EKAIGEVFDL RPAAIVRDLD LLRPIYAPTA AYGHFGRTDI ELPWEQTNKV DDLKSAI
Validated NT Sequence
cnnnnnnnnn nnntgtnagc agccggatcc tcgagaagct tggctgcaga acttgttcgt gctgtttatt agatggcgga cttcaggtcg tccaccttgt tggtctgctc ccacggcagc tcgatgtcgg tgcggccgaa gtgtccgtac gcggcggtgg gcgcgtagat cgggcgcagc aggtcgaggt cgcgcacgat cgcggcggga cgcaggtcga acacctcgcc gatggccttc tcgatcttgg ccgggtcgac ggtctcgctg ccgaaggtct ccacgaacag acccaccggg gcggccttgc cgatcgcgta ggcgacctgc acctcgacgc gctcggccag gccagcggcg acgacgttct tggcgaccca gcgcatcgcg taggcggccg agcggtcgac ctttgacgga tccttgcccg agaaggcgcc gccgccgtgg cgagcccagc cgccgtaggt gtcgacgatg atcttgcggc cggtcaggcc ggcgtcgccc atggggccgc cgaggacgaa cttgcccgtc gggttgacca gcaggcggta gtcggacgtg tcgagggtct catggccgag atcggcgagc acggtgttga cgaccttctc gcggatgtcg ggcgtgaggg tgccctccag gtcgatgccg tcggcgtgct gggtggacag caccacggtg tccaaccgca ccggggtggt gccgtcgtac tggatggtga cctgggtctt gccgtcnggc cgcagtagtc cagcacaccg ttcttgcgca cctcggtcag gcggcgcgcg aggcggtggg ccagcgcgat gggcagcggc atgagttcgg gggtgtcgcc gatggcgtan ccnaacatcn nnccctggtc gcctgcgccc tgcaggtcca gcgggtcggc ntngccnncg acgcgggtct cnnngggcgn ntcnacgcnn tgggcgangn nggnnactgn ncncnatcnn nnnnnnacnn ncnnnncnan gnnncnncna nnnnnngnng annanncnta nnnnnntcnn ngnngnnnnn nnnnnnatnn nggnnnntnn nnnnnnnncn nnnngncnnn nnnnnnnnnn nncnnnnnnn nnnnacnnnn nnnnnnnngn nnnngnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngnnnnann nnt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSKGRLFT SESVTEGHPD KICDAISDSV LDALLEQDPK SRVAVETLVT TGQVHVAGEV TTTAYADIPK IVRDRILDIG YDSSTKGFDG ASCGVNVAIG AQSPDIAQGV DTAHETRVEG KADPLDLQGA GDQGLMFGYA IGDTPELMPL PIALAHRLAR RLTEVRKNGV LDYLRPDGKT QVTIQYDGTT PVRLDTVVLS TQHADGIDLE GTLTPDIREK VVNTVLADLG HETLDTSDYR LLVNPTGKFV LGGPMGDAGL TGRKIIVDTY GGWARHGGGA FSGKDPSKVD RSAAYAMRWV AKNVVAAGLA ERVEVQVAYA IGKAAPVGLF VETFGSETVD PAKIEKAIGE VFDLRPAAIV RDLDLLRPIY APTAAYGHFG RTDIELPWEQ TNKVDDLKSA I
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCAAA GGTCGCCTGT TTACCAGTGA GTCGGTAACC GAAGGGCACC CCGACAAGAT CTGTGACGCA ATCAGCGACT CTGTGCTCGA CGCGCTGCTG GAGCAGGATC CCAAGTCCCG CGTCGCCGTC GAGACGCTGG TCACCACGGG CCAGGTGCAC GTCGCCGGTG AGGTGACCAC CACGGCGTAC GCCGACATCC CCAAGATCGT GCGCGACCGC ATCCTCGACA TCGGCTACGA CTCGTCGACC AAGGGCTTCG ACGGCGCCTC GTGCGGCGTC AACGTGGCGA TCGGTGCGCA GTCGCCCGAC ATCGCCCAAG GCGTCGACAC CGCCCACGAG ACCCGCGTCG AGGGCAAGGC CGACCCGCTG GACCTGCAGG GCGCAGGCGA CCAGGGCCTG ATGTTCGGCT ACGCCATCGG CGACACCCCC GAACTCATGC CGCTGCCCAT CGCGCTGGCC CACCGCCTCG CGCGCCGCCT GACCGAGGTG CGCAAGAACG GTGTGCTGGA CTACCTGCGG CCCGACGGCA AGACCCAGGT CACCATCCAG TACGACGGCA CCACCCCGGT GCGGTTGGAC ACCGTGGTGC TGTCCACCCA GCACGCCGAC GGCATCGACC TGGAGGGCAC CCTCACGCCC GACATCCGCG AGAAGGTCGT CAACACCGTG CTCGCCGATC TCGGCCATGA GACCCTCGAC ACGTCCGACT ACCGCCTGCT GGTCAACCCG ACGGGCAAGT TCGTCCTCGG CGGCCCCATG GGCGACGCCG GCCTGACCGG CCGCAAGATC ATCGTCGACA CCTACGGCGG CTGGGCTCGC CACGGCGGCG GCGCCTTCTC GGGCAAGGAT CCGTCAAAGG TCGACCGCTC GGCCGCCTAC GCGATGCGCT GGGTCGCCAA GAACGTCGTC GCCGCTGGCC TGGCCGAGCG CGTCGAGGTG CAGGTCGCCT ACGCGATCGG CAAGGCCGCC CCGGTGGGTC TGTTCGTGGA GACCTTCGGC AGCGAGACCG TCGACCCGGC CAAGATCGAG AAGGCCATCG GCGAGGTGTT CGACCTGCGT CCCGCCGCGA TCGTGCGCGA CCTCGACCTG CTGCGCCCGA TCTACGCGCC CACCGCCGCG TACGGACACT TCGGCCGCAC CGACATCGAG CTGCCGTGGG AGCAGACCAA CAAGGTGGAC GACCTGAAGT CCGCCATCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA