MyleA.01625.a

S-adenosylmethionine synthetase

CENTER ID: MyleA.01625.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01625.a.A1.GE29271 Full length( MyleA.01625.a ) 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.940
RefSeq: YP_002503094.1
UniProt: B8ZUM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEKGRLFTS ESVTEGHPDK ICDAISDSIL DALLAEDPCS RVAVETLVTT GQVHVVGEVT TLAKTAFADI SNTVRERILD IGYDSSDKGF DGASCGVNIG IGAQSSDIAQ GVNTAHEVRV EGAADPLDAQ GAGDQGLMFG YAINDTPELM PLPIALAHRL ARRLTEVRKN GVLPYLRSDG KTQVTIAYED NVPVRLDTVV ISTQHAAGVD LDATLAPDIR EKVLNTVIDD LSHDTLDVSS VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR WVAKNIVAAG LAERIEVQVA YAIGKAAPVG LFVETFGTEA VDPAKIEKAI GEVFDLRPGA IIRDLHLLRP IYAQTAAYGH FGRTDVELPW EQLNKVDDLK RAI
NT Sequence
GTGAGTGAGA AGGGTCGGCT GTTCACTAGC GAGTCGGTGA CTGAGGGACA TCCCGACAAG ATCTGTGATG CGATCAGCGA CTCGATCCTT GACGCACTTT TGGCGGAGGA TCCTTGCTCA CGTGTCGCGG TCGAGACGTT GGTCACCACC GGGCAGGTGC ATGTGGTGGG TGAAGTGACG ACGTTGGCCA AGACGGCGTT CGCTGATATC AGTAATACGG TCCGCGAACG TATTCTCGAT ATCGGCTACG ACTCGTCGGA CAAGGGCTTC GATGGGGCGT CGTGCGGAGT TAACATTGGC ATCGGCGCTC AGTCGTCTGA CATTGCTCAA GGCGTCAATA CCGCCCATGA AGTACGCGTC GAGGGCGCGG CGGATCCGCT GGACGCCCAG GGTGCTGGTG ACCAAGGCCT GATGTTCGGT TACGCGATCA ATGACACCCC GGAACTGATG CCGCTACCGA TTGCACTGGC CCACCGACTG GCGCGAAGGC TGACCGAGGT ACGCAAGAAC GGCGTGCTGC CCTACCTGCG TTCCGACGGC AAGACCCAGG TCACTATCGC CTACGAGGAC AATGTCCCAG TGCGTTTGGA CACTGTGGTC ATCTCCACTC AGCACGCCGC TGGTGTCGAC CTGGATGCCA CGCTGGCTCC TGATATCCGG GAGAAGGTGC TCAACACCGT TATTGACGAT CTGTCTCATG ACACCTTGGA TGTATCGTCG GTGCGGGTGC TGGTAAACCC GACCGGCAAG TTCGTGCTAG GTGGGCCGAT GGGCGATGCC GGGCTCACCG GTCGCAAGAT CATCGTCGAC ACCTACGGTG GCTGGGCGCG TCACGGCGGC GGCGCCTTCT CTGGCAAGGA TCCGTCCAAG GTGGACCGGT CGGCAGCCTA CGCGATGCGC TGGGTGGCCA AGAACATCGT CGCTGCCGGG CTGGCGGAGC GAATCGAGGT GCAGGTGGCA TACGCCATCG GCAAAGCCGC CCCGGTCGGT TTGTTCGTCG AGACCTTTGG CACTGAGGCG GTCGATCCGG CCAAAATCGA GAAAGCCATC GGCGAGGTGT TCGATCTGCG TCCCGGCGCG ATCATCCGCG ACCTGCATCT GCTGCGCCCA ATTTACGCGC AAACCGCTGC CTATGGGCAC TTCGGTCGCA CTGACGTCGA ACTGCCATGG GAGCAGCTCA ACAAAGTCGA CGATCTCAAG CGCGCCATC
Details for MyleA.01625.a.A1.GE29271
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVSEKGRLFT SESVTEGHPD KICDAISDSI LDALLAEDPC SRVAVETLVT TGQVHVVGEV TTLAKTAFAD ISNTVRERIL DIGYDSSDKG FDGASCGVNI GIGAQSSDIA QGVNTAHEVR VEGAADPLDA QGAGDQGLMF GYAINDTPEL MPLPIALAHR LARRLTEVRK NGVLPYLRSD GKTQVTIAYE DNVPVRLDTV VISTQHAAGV DLDATLAPDI REKVLNTVID DLSHDTLDVS SVRVLVNPTG KFVLGGPMGD AGLTGRKIIV DTYGGWARHG GGAFSGKDPS KVDRSAAYAM RWVAKNIVAA GLAERIEVQV AYAIGKAAPV GLFVETFGTE AVDPAKIEKA IGEVFDLRPG AIIRDLHLLR PIYAQTAAYG HFGRTDVELP WEQLNKVDDL KRA
Validated NT Sequence
gatggcgcgc ttgagatcgt cgactttgtt gagctgctcc catggcagtt cgacgtcagt gcgaccgaag tgcccatagg cagcggtttg cgcgtaaatt gggcgcagca gatgcaggtc gcggatgatc gcgccgggac gcagatcgaa cacctcgccg atggctttct cgattttggc cggatcgacc gcctcagtgc caaaggtctc gacgaacaaa ccgaccgggg cggctttgcc gatggcgtat gccacctgca cctcgattcg ctccgccagc ccggcagcga cgatgttctt ggccacccag cgcatcgcgt aggctgccga ccggtccacc ttggacggat ccttgccaga gaaggcgccg ccgccgtgac gcgcccagcc accgtaggtg tcgacgatga tcttgcgacc ggtgagcccg gcatcgccca tcggcccacc tagcacgaac ttgccggtcg ggtttaccag cacccgcacc gacgatacat ccaaggtgtc atgagacaga tcgtcaataa cggtgttgag caccttctcc cggatatcag gagccagcgt ggcatccagg tcgacaccag cggcgtgctg agtggagatg accacagtgt ccaaacgcac tgggacattg tcctcgtagg cgatagtgac ctgggtcttg ccgtcggaac gcaggtaggg cagcacgccg ttcttgcgta cctcggtcag ccttcgcgcc agtcggtggg ccagtgcaat cggtagcggc atcagttccg gggtgtcatt gatcgcgtaa ccgaacatca ggccttggtc accagcaccc tgggcgtcca gcggatccgc cgcgccctcg acgcgtactt catgggcggt attgacgcct tgagcaatgt cagacgactg agcgccgatg ccaatgttaa ctccgcacga cgccccatcg aagcccttgt ccgacgagtc gtagccgata tcgagaatac gttcgcggac cgtattactg atatcagcga acgccgtctt ggccaacgtc gtcacttcac ccaccacatg cacctgcccg gtggtgacca acgtctcgac cgcgacacgt gagcaaggat cctccgccaa aagtgcgtca aggatcgagt cgctgatcgc atcacagatc ttgtcgggat gtccctcagt caccgactcg ctagtgaaca gccgaccctt ctcactcacc atcgaaccag gaccctgggt ctgagcttcc aggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEKGRLF TSESVTEGHP DKICDAISDS ILDALLAEDP CSRVAVETLV TTGQVHVVGE VTTLAKTAFA DISNTVRERI LDIGYDSSDK GFDGASCGVN IGIGAQSSDI AQGVNTAHEV RVEGAADPLD AQGAGDQGLM FGYAINDTPE LMPLPIALAH RLARRLTEVR KNGVLPYLRS DGKTQVTIAY EDNVPVRLDT VVISTQHAAG VDLDATLAPD IREKVLNTVI DDLSHDTLDV SSVRVLVNPT GKFVLGGPMG DAGLTGRKII VDTYGGWARH GGGAFSGKDP SKVDRSAAYA MRWVAKNIVA AGLAERIEVQ VAYAIGKAAP VGLFVETFGT EAVDPAKIEK AIGEVFDLRP GAIIRDLHLL RPIYAQTAAY GHFGRTDVEL PWEQLNKVDD LKRAI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgag aagggtcggc tgttcactag cgagtcggtg actgagggac atcccgacaa gatctgtgat gcgatcagcg actcgatcct tgacgcactt ttggcggagg atccttgctc acgtgtcgcg gtcgagacgt tggtcaccac cgggcaggtg catgtggtgg gtgaagtgac gacgttggcc aagacggcgt tcgctgatat cagtaatacg gtccgcgaac gtattctcga tatcggctac gactcgtcgg acaagggctt cgatggggcg tcgtgcggag ttaacattgg catcggcgct cagtcgtctg acattgctca aggcgtcaat accgcccatg aagtacgcgt cgagggcgcg gcggatccgc tggacgccca gggtgctggt gaccaaggcc tgatgttcgg ttacgcgatc aatgacaccc cggaactgat gccgctaccg attgcactgg cccaccgact ggcgcgaagg ctgaccgagg tacgcaagaa cggcgtgctg ccctacctgc gttccgacgg caagacccag gtcactatcg cctacgagga caatgtccca gtgcgtttgg acactgtggt catctccact cagcacgccg ctggtgtcga cctggatgcc acgctggctc ctgatatccg ggagaaggtg ctcaacaccg ttattgacga tctgtctcat gacaccttgg atgtatcgtc ggtgcgggtg ctggtaaacc cgaccggcaa gttcgtgcta ggtgggccga tgggcgatgc cgggctcacc ggtcgcaaga tcatcgtcga cacctacggt ggctgggcgc gtcacggcgg cggcgccttc tctggcaagg atccgtccaa ggtggaccgg tcggcagcct acgcgatgcg ctgggtggcc aagaacatcg tcgctgccgg gctggcggag cgaatcgagg tgcaggtggc atacgccatc ggcaaagccg ccccggtcgg tttgttcgtc gagacctttg gcactgaggc ggtcgatccg gccaaaatcg agaaagccat cggcgaggtg ttcgatctgc gtcccggcgc gatcatccgc gacctgcatc tgctgcgccc aatttacgcg caaaccgctg cctatgggca cttcggtcgc actgacgtcg aactgccatg ggagcagctc aacaaagtcg acgatctcaa gcgcgccatc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa