MyulA.01625.a

S-adenosylmethionine synthetase

CENTER ID: MyulA.01625.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01625.a.A1.GE29941 Full length( MyulA.01625.a ) 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2144
RefSeq: YP_905738.1
UniProt: A0PPJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEKGRLFTS ESVTEGHPDK ICDAVSDSVL DALLAADPRS RVAVETLVTT GQVHVVGEVT TTAKEAFADI TNIVRERILD IGYDSSDKGF DGASCGVNIG IGAQSPDIAQ GVDTAHEARV EGAADPLDAQ GAGDQGLMFG YAINDTPELM PLPIALAHRL SRRLTEVRKN GVLPYLRPDG KTQVTIAYED RVPVRLDTVV ISTQHADDID LVKTLDPDIR EQVLKTVLDD LAHDTLDASA VRVLVNPTGK FVLGGPMGDA GLTGRKIIVD TYGGWARHGG GAFSGKDPSK VDRSAAYAMR WVAKNVVAAG LAEGVEVQVA YAIGKAAPVG LFVETFGSEA VDPVKIEKAI GEVFDLRPGA IIRDLNLLRP IYAPTAAYGH FGRTDVDLPW ERLDKVDDLK RAI
NT Sequence
GTGAGTGAAA AGGGTCGCCT TTTTACCAGT GAGTCGGTGA CGGAAGGACA TCCCGACAAG ATCTGTGACG CGGTCAGCGA CTCGGTCCTC GACGCGCTTC TGGCGGCGGA CCCCCGCTCG CGTGTTGCAG TTGAGACCCT GGTGACCACC GGGCAGGTGC ACGTGGTGGG TGAGGTCACG ACGACCGCCA AGGAGGCGTT CGCTGACATC ACCAATATCG TTCGCGAACG CATCCTCGAC ATCGGCTACG ACTCGTCGGA CAAGGGCTTC GACGGGGCAT CCTGCGGAGT GAACATCGGT ATCGGAGCCC AGTCGCCCGA CATCGCACAA GGTGTTGATA CCGCCCATGA GGCCCGCGTC GAGGGCGCTG CGGATCCGTT GGACGCGCAA GGCGCCGGCG ACCAGGGCCT GATGTTCGGT TACGCGATCA ACGACACCCC GGAGCTGATG CCGCTGCCGA TCGCGCTGGC TCACCGGTTG TCGCGGCGAC TGACCGAGGT CCGCAAGAAC GGTGTGCTGC CCTACTTGCG CCCGGATGGC AAGACTCAGG TCACCATCGC CTACGAGGAC CGGGTGCCGG TGCGGCTGGA CACGGTGGTC ATTTCGACCC AGCATGCGGA CGACATCGAC CTGGTGAAGA CGCTGGATCC CGACATTCGC GAGCAGGTGC TCAAGACCGT GCTCGACGAC CTGGCCCACG ACACCCTGGA TGCCTCGGCG GTGCGGGTGC TGGTCAACCC GACCGGAAAG TTCGTCCTTG GCGGACCGAT GGGCGATGCG GGCCTCACCG GCCGCAAGAT CATCGTCGAC ACCTACGGCG GGTGGGCCCG GCACGGTGGT GGCGCATTCT CCGGCAAGGA TCCGTCCAAG GTGGACCGCT CGGCGGCGTA CGCGATGCGC TGGGTGGCCA AAAACGTGGT CGCAGCAGGA CTGGCCGAGG GGGTCGAGGT ACAGGTGGCC TACGCCATCG GTAAAGCCGC CCCGGTCGGC CTGTTCGTGG AGACATTCGG CTCCGAGGCT GTCGACCCGG TCAAGATCGA GAAGGCCATC GGTGAGGTGT TCGACCTGCG GCCTGGCGCG ATCATTCGCG ACCTGAACCT GCTGCGGCCC ATCTATGCGC CCACCGCCGC TTACGGGCAC TTCGGTCGCA CCGATGTCGA CCTGCCGTGG GAGCGGCTCG ACAAGGTCGA CGACCTCAAG CGCGCCATC
Details for MyulA.01625.a.A1.GE29941
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 45kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMVSEKGR LFTSESVTEG HPDKICDAVS DSVLDALLAA DPRSRVAVET LVTTGQVHVV GEVTTTAKEA FADITNIVRE RILDIGYDSS DKGFDGASCG VNIGIGAQSP DIAQGVDTAH EARVEGAADP LDAQGAGDQG LMFGYAINDT PELMPLPIAL AHRLSRRLTE VRKNGVLPYL RPDGKTQVTI AYEDRVPVRL DTVVISTQHA DDIDLVKTLD PDIREQVLKT VLDDLAHDTL DASAVRVLVN PTGKFVLGGP MGDAGLTGRK IIVDTYGGWA RHGGGAFSGK DPSKVDRSAA YAMRWVAKNV VAAGLAEGVE VQVAYAIGKA APVGLFVETF GSEAVDPVKI EKAIGEVFDL RPGAIIRDLN LLRPIYAPTA AYGHFGRTDV DLPWERLDKV DDLKRA
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagt gaaaagggtc gcctttttac cagtgagtcg gtgacggaag gacatcccga caagatctgt gacgcggtca gcgactcggt cctcgacgcg cttctggcgg cggacccccg ctcgcgtgtt gcagttgaga ccctggtgac caccgggcag gtgcacgtgg tgggtgaggt cacgacgacc gccaaggagg cgttcgctga catcaccaat atcgttcgcg aacgcatcct cgacatcggc tacgactcgt cggacaaggg cttcgacggg gcatcctgcg gagtgaacat cggtatcgga gcccagtcgc ccgacatcgc acaaggtgtt gataccgccc atgaggcccg cgtcgagggc gctgcggatc cgttggacgc gcaaggcgcc ggcgaccagg gcctgatgtt cggttacgcg atcaacgaca ccccggagct gatgccgctg ccgatcgcgc tggctcaccg gttgtcgcgg cgactgaccg aggtccgcaa gaacggtgtg ctgccctact tgcgcccgga tggcaagact caggtcacca tcgcctacga ggaccgggtg ccggtgcggc tggacacggt ggtcatttcg acccagcatg cggacgacat cgacctggtg aagacgctgg atcccgacat tcgcgagcag gtgctcaaga ccgtgctcga cgacctggcc cacgacaccc tggatgcctc ggcggtgcgg gtgctggtca acccgaccgg aaagttcgtc cttggcggac cgatgggcga tgcgggcctc accggccgca agatcatcgt cgacacctac ggcgggtggg cccggcacgg tggtggcgca ttctccggca aggatccgtc caaggtggac cgctcggcgg cgtacgcgat gcgctgggtg gccaaaaacg tggtcgcagc aggactggcc gagggggtcg aggtacaggt ggcctacgcc atcggtaaag ccgccccggt cggcctgttc gtggagacat tcggctccga ggctgtcgac ccggtcaaga tcgagaaggc catcggtgag gtgttcgacc tgcggcctgg cgcgatcatt cgcgacctga acctgctgcg gcccatctat gcgcccaccg ccgcttacgg gcacttcggt cgcaccgatg tcgacctgcc gtgggagcgg ctcgacaagg tcgacgacct caagcgcgcc taat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSEKGRLF TSESVTEGHP DKICDAVSDS VLDALLAADP RSRVAVETLV TTGQVHVVGE VTTTAKEAFA DITNIVRERI LDIGYDSSDK GFDGASCGVN IGIGAQSPDI AQGVDTAHEA RVEGAADPLD AQGAGDQGLM FGYAINDTPE LMPLPIALAH RLSRRLTEVR KNGVLPYLRP DGKTQVTIAY EDRVPVRLDT VVISTQHADD IDLVKTLDPD IREQVLKTVL DDLAHDTLDA SAVRVLVNPT GKFVLGGPMG DAGLTGRKII VDTYGGWARH GGGAFSGKDP SKVDRSAAYA MRWVAKNVVA AGLAEGVEVQ VAYAIGKAAP VGLFVETFGS EAVDPVKIEK AIGEVFDLRP GAIIRDLNLL RPIYAPTAAY GHFGRTDVDL PWERLDKVDD LKRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtgaa aagggtcgcc tttttaccag tgagtcggtg acggaaggac atcccgacaa gatctgtgac gcggtcagcg actcggtcct cgacgcgctt ctggcggcgg acccccgctc gcgtgttgca gttgagaccc tggtgaccac cgggcaggtg cacgtggtgg gtgaggtcac gacgaccgcc aaggaggcgt tcgctgacat caccaatatc gttcgcgaac gcatcctcga catcggctac gactcgtcgg acaagggctt cgacggggca tcctgcggag tgaacatcgg tatcggagcc cagtcgcccg acatcgcaca aggtgttgat accgcccatg aggcccgcgt cgagggcgct gcggatccgt tggacgcgca aggcgccggc gaccagggcc tgatgttcgg ttacgcgatc aacgacaccc cggagctgat gccgctgccg atcgcgctgg ctcaccggtt gtcgcggcga ctgaccgagg tccgcaagaa cggtgtgctg ccctacttgc gcccggatgg caagactcag gtcaccatcg cctacgagga ccgggtgccg gtgcggctgg acacggtggt catttcgacc cagcatgcgg acgacatcga cctggtgaag acgctggatc ccgacattcg cgagcaggtg ctcaagaccg tgctcgacga cctggcccac gacaccctgg atgcctcggc ggtgcgggtg ctggtcaacc cgaccggaaa gttcgtcctt ggcggaccga tgggcgatgc gggcctcacc ggccgcaaga tcatcgtcga cacctacggc gggtgggccc ggcacggtgg tggcgcattc tccggcaagg atccgtccaa ggtggaccgc tcggcggcgt acgcgatgcg ctgggtggcc aaaaacgtgg tcgcagcagg actggccgag ggggtcgagg tacaggtggc ctacgccatc ggtaaagccg ccccggtcgg cctgttcgtg gagacattcg gctccgaggc tgtcgacccg gtcaagatcg agaaggccat cggtgaggtg ttcgacctgc ggcctggcgc gatcattcgc gacctgaacc tgctgcggcc catctatgcg cccaccgccg cttacgggca cttcggtcgc accgatgtcg acctgccgtg ggagcggctc gacaaggtcg acgacctcaa gcgcgccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa