MytuD.01331.a

phosphoglycerate kinase

CENTER ID: MytuD.01331.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01331.a.A1.GE25957 Full length( MytuD.01331.a ) 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01331.a.A1.PW25975 Full length( MytuD.01331.a ) 1 412

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1601
RefSeq: NP_215953.1
TubercuList: Rv1437
MTB Network Portal: Rv1437
UniProt: P9WID1

Orthologues for Rv1437

Species % Identity % Coverage Clones Proteins Structures
marinum 86 99 1 1 0
ulcerans 85 99 1 0 0
avium 82 100 2 3 1
leprae 80 100 0 0 0
smegmatis 77 98 1 1 0
abscessus 73 98 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVANLKDLL AEGVSGRGVL VRSDLNVPLD EDGTITDAGR IIASAPTLKA LLDADAKVVV AAHLGRPKDG PDPTLSLAPV AVALGEQLGR HVQLAGDVVG ADALARAEGL TGGDILLLEN IRFDKRETSK NDDDRRALAK QLVELVGTGG VFVSDGFGVV HRKQASVYDI ATLLPHYAGT LVADEMRVLE QLTSSTQRPY AVVLGGSKVS DKLGVIESLA TKADSIVIGG GMCFTFLAAQ GFSVGTSLLE DDMIEVCRGL LETYHDVLRL PVDLVVTEKF AADSPPQTVD VGAVPNGLMG LDIGPGSIKR FSTLLSNAGT IFWNGPMGVF EFPAYAAGTR GVAEAIVAAT GKGAFSVVGG GDSAAAVRAM NIPEGAFSHI STGGGASLEY LEGKTLPGIE VLSREQPTGG VL
NT Sequence
ATGAGCGTTG CAAACCTCAA GGATCTACTC GCCGAAGGTG TTTCGGGGCG TGGAGTGCTG GTGCGCTCCG ATCTCAACGT TCCGCTCGAC GAGGACGGCA CCATTACCGA TGCGGGCCGC ATCATCGCGT CGGCGCCGAC GTTGAAGGCG TTGCTCGACG CCGACGCCAA GGTGGTGGTT GCCGCGCACT TGGGACGTCC CAAGGACGGG CCGGACCCGA CACTGTCGCT GGCGCCGGTC GCCGTGGCGC TGGGTGAGCA ACTCGGCCGG CACGTCCAGC TGGCTGGAGA CGTTGTCGGC GCCGATGCGC TGGCCCGCGC CGAGGGGCTC ACCGGCGGCG ACATCCTGCT GCTGGAGAAC ATCCGCTTCG ACAAACGCGA AACCAGCAAG AACGATGACG ACCGGCGGGC ACTGGCCAAG CAGCTGGTCG AACTGGTCGG AACGGGAGGC GTTTTCGTCT CCGACGGCTT TGGGGTGGTG CACCGCAAGC AAGCCTCGGT CTATGACATC GCAACCCTGT TGCCGCACTA CGCCGGCACG CTGGTCGCCG ACGAGATGCG GGTACTGGAG CAGTTGACCA GCTCGACCCA GCGGCCCTAT GCGGTAGTGC TCGGCGGATC AAAGGTGTCC GACAAGCTGG GTGTCATCGA GTCGCTGGCG ACCAAGGCGG ACAGCATTGT GATTGGCGGC GGAATGTGCT TCACATTCCT TGCTGCACAG GGATTTTCGG TTGGCACATC GCTGCTGGAA GACGACATGA TCGAAGTCTG TCGCGGGCTG CTGGAAACCT ATCACGACGT GTTGCGGCTG CCCGTGGATC TAGTGGTCAC GGAGAAGTTC GCCGCCGACT CGCCGCCCCA GACGGTCGAC GTCGGCGCTG TGCCCAATGG CTTGATGGGC CTGGATATCG GGCCGGGATC GATCAAACGG TTCAGCACGC TGCTGTCCAA CGCCGGGACC ATCTTCTGGA ACGGGCCGAT GGGAGTATTC GAGTTCCCGG CTTATGCGGC CGGCACCAGA GGCGTCGCCG AGGCGATCGT CGCCGCCACC GGCAAAGGGG CGTTTAGTGT GGTCGGCGGC GGTGACTCCG CGGCCGCAGT GCGCGCGATG AACATCCCCG AGGGCGCCTT CTCACACATA TCCACCGGCG GCGGTGCCTC GCTGGAATAC CTTGAGGGCA AGACGCTTCC CGGCATCGAG GTACTGAGCC GTGAGCAGCC AACCGGAGGA GTTTTG
Details for MytuD.01331.a.A1.GE25957
HARVESTED ON: 1/9/2009
SEQUENCED ON: 6/5/2012
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVANLKDL LAEGVSGRGV LVRSDLNVPL DEDGTITDAG RIIASAPTLK ALLDADAKVV VAAHLGRPKD GPDPTLSLAP VAVALGEQLG RHVQLAGDVV GADALARAEG LTGGDILLLE NIRFDKRETS KNDDDRRALA KQLVELVGTG GVFVSDGFGV VHRKQASVYD IATLLPHYAG TLVADEMRVL EQLTSSTQRP YAVVLGGSKV SDKLGVIESL ATKADSIVIG GGMCFTFLAA QGFSVGTSLL EDDMIEVCRG LLETYHDVLR LPVDLVVTEK FAADSPPQTV DVGAVPNGLM GLDIGPGSIK RFSTLLSNAG TIFWNGPMGV FEFPAYAAGT RGVAEAIVAA TGKGAFSVVG GGDSAAAVRA MNIPEGAFSH ISTGGGASLE YLEGKTLPGI EVLSREQPTG GVL
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgttgca aacctcaagg atctactcgc cgaaggtgtt tcggggcgtg gagtgctggt gcgctccgat ctcaacgttc cgctcgacga ggacggcacc attaccgatg cgggccgcat catcgcgtcg gcgccgacgt tgaaggcgtt gctcgacgcc gacgccaagg tggtggttgc cgcgcacttg ggacgtccca aggacgggcc ggacccgaca ctgtcgctgg cgccggtcgc cgtggcgctg ggtgagcaac tcggccggca cgtccagctg gctggagacg ttgtcggcgc cgatgcgctg gcccgcgccg aggggctcac cggcggcgac atcctgctgc tggagaacat ccgcttcgac aaacgcgaaa ccagcaagaa cgatgacgac cggcgggcac tggccaagca gctggtcgaa ctggtcggaa cgggaggcgt tttcgtctcc gacggctttg gggtggtgca ccgcaagcaa gcctcggtct atgacatcgc aaccctgttg ccgcactacg ccggcacgct ggtcgccgac gagatgcggg tactggagca gttgaccagc tcgacccagc ggccctatgc ggtagtgctc ggcggatcaa aggtgtccga caagctgggt gtcatcgagt cgctggcgac caaggcggac agcattgtga ttggcggcgg aatgtgcttc acattccttg ctgcacaggg attttcggtt ggcacatcgc tgctggaaga cgacatgatc gaagtctgtc gcgggctgct ggaaacctat cacgacgtgt tgcggctgcc cgtggatcta gtggtcacgg agaagttcgc cgccgactcg ccgccccaga cggtcgacgt cggcgctgtg cccaatggct tgatgggcct ggatatcggg ccgggatcga tcaaacggtt cagcacgctg ctgtccaacg ccgggaccat cttctggaac gggccgatgg gagtattcga gttcccggct tatgcggccg gcaccagagg cgtcgccgag gcgatcgtcg ccgccaccgg caaaggggcg tttagtgtgg tcggcggcgg tgactccgcg gccgcagtgc gcgcgatgaa catccccgag ggcgccttct cacacatatc caccggcggc ggtgcctcgc tggaatacct tgagggcaag acgcttcccg gcatcgaggt actgagccgt gagcagccaa ccggaggagt tttgtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVANLKDL LAEGVSGRGV LVRSDLNVPL DEDGTITDAG RIIASAPTLK ALLDADAKVV VAAHLGRPKD GPDPTLSLAP VAVALGEQLG RHVQLAGDVV GADALARAEG LTGGDILLLE NIRFDKRETS KNDDDRRALA KQLVELVGTG GVFVSDGFGV VHRKQASVYD IATLLPHYAG TLVADEMRVL EQLTSSTQRP YAVVLGGSKV SDKLGVIESL ATKADSIVIG GGMCFTFLAA QGFSVGTSLL EDDMIEVCRG LLETYHDVLR LPVDLVVTEK FAADSPPQTV DVGAVPNGLM GLDIGPGSIK RFSTLLSNAG TIFWNGPMGV FEFPAYAAGT RGVAEAIVAA TGKGAFSVVG GGDSAAAVRA MNIPEGAFSH ISTGGGASLE YLEGKTLPGI EVLSREQPTG GVL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgttgca aacctcaagg atctactcgc cgaaggtgtt tcggggcgtg gagtgctggt gcgctccgat ctcaacgttc cgctcgacga ggacggcacc attaccgatg cgggccgcat catcgcgtcg gcgccgacgt tgaaggcgtt gctcgacgcc gacgccaagg tggtggttgc cgcgcacttg ggacgtccca aggacgggcc ggacccgaca ctgtcgctgg cgccggtcgc cgtggcgctg ggtgagcaac tcggccggca cgtccagctg gctggagacg ttgtcggcgc cgatgcgctg gcccgcgccg aggggctcac cggcggcgac atcctgctgc tggagaacat ccgcttcgac aaacgcgaaa ccagcaagaa cgatgacgac cggcgggcac tggccaagca gctggtcgaa ctggtcggaa cgggaggcgt tttcgtctcc gacggctttg gggtggtgca ccgcaagcaa gcctcggtct atgacatcgc aaccctgttg ccgcactacg ccggcacgct ggtcgccgac gagatgcggg tactggagca gttgaccagc tcgacccagc ggccctatgc ggtagtgctc ggcggatcaa aggtgtccga caagctgggt gtcatcgagt cgctggcgac caaggcggac agcattgtga ttggcggcgg aatgtgcttc acattccttg ctgcacaggg attttcggtt ggcacatcgc tgctggaaga cgacatgatc gaagtctgtc gcgggctgct ggaaacctat cacgacgtgt tgcggctgcc cgtggatcta gtggtcacgg agaagttcgc cgccgactcg ccgccccaga cggtcgacgt cggcgctgtg cccaatggct tgatgggcct ggatatcggg ccgggatcga tcaaacggtt cagcacgctg ctgtccaacg ccgggaccat cttctggaac gggccgatgg gagtattcga gttcccggct tatgcggccg gcaccagagg cgtcgccgag gcgatcgtcg ccgccaccgg caaaggggcg tttagtgtgg tcggcggcgg tgactccgcg gccgcagtgc gcgcgatgaa catccccgag ggcgccttct cacacatatc caccggcggc ggtgcctcgc tggaatacct tgagggcaag acgcttcccg gcatcgaggt actgagccgt gagcagccaa ccggaggagt tttgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MytuD.01331.a.A1.PW25975
PURIFICATION DATe: 3/31/2009
CONCENTRATION: 22.96mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVANLKDL LAEGVSGRGV LVRSDLNVPL DEDGTITDAG RIIASAPTLK ALLDADAKVV VAAHLGRPKD GPDPTLSLAP VAVALGEQLG RHVQLAGDVV GADALARAEG LTGGDILLLE NIRFDKRETS KNDDDRRALA KQLVELVGTG GVFVSDGFGV VHRKQASVYD IATLLPHYAG TLVADEMRVL EQLTSSTQRP YAVVLGGSKV SDKLGVIESL ATKADSIVIG GGMCFTFLAA QGFSVGTSLL EDDMIEVCRG LLETYHDVLR LPVDLVVTEK FAADSPPQTV DVGAVPNGLM GLDIGPGSIK RFSTLLSNAG TIFWNGPMGV FEFPAYAAGT RGVAEAIVAA TGKGAFSVVG GGDSAAAVRA MNIPEGAFSH ISTGGGASLE YLEGKTLPGI EVLSREQPTG GVL
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgttgca aacctcaagg atctactcgc cgaaggtgtt tcggggcgtg gagtgctggt gcgctccgat ctcaacgttc cgctcgacga ggacggcacc attaccgatg cgggccgcat catcgcgtcg gcgccgacgt tgaaggcgtt gctcgacgcc gacgccaagg tggtggttgc cgcgcacttg ggacgtccca aggacgggcc ggacccgaca ctgtcgctgg cgccggtcgc cgtggcgctg ggtgagcaac tcggccggca cgtccagctg gctggagacg ttgtcggcgc cgatgcgctg gcccgcgccg aggggctcac cggcggcgac atcctgctgc tggagaacat ccgcttcgac aaacgcgaaa ccagcaagaa cgatgacgac cggcgggcac tggccaagca gctggtcgaa ctggtcggaa cgggaggcgt tttcgtctcc gacggctttg gggtggtgca ccgcaagcaa gcctcggtct atgacatcgc aaccctgttg ccgcactacg ccggcacgct ggtcgccgac gagatgcggg tactggagca gttgaccagc tcgacccagc ggccctatgc ggtagtgctc ggcggatcaa aggtgtccga caagctgggt gtcatcgagt cgctggcgac caaggcggac agcattgtga ttggcggcgg aatgtgcttc acattccttg ctgcacaggg attttcggtt ggcacatcgc tgctggaaga cgacatgatc gaagtctgtc gcgggctgct ggaaacctat cacgacgtgt tgcggctgcc cgtggatcta gtggtcacgg agaagttcgc cgccgactcg ccgccccaga cggtcgacgt cggcgctgtg cccaatggct tgatgggcct ggatatcggg ccgggatcga tcaaacggtt cagcacgctg ctgtccaacg ccgggaccat cttctggaac gggccgatgg gagtattcga gttcccggct tatgcggccg gcaccagagg cgtcgccgag gcgatcgtcg ccgccaccgg caaaggggcg tttagtgtgg tcggcggcgg tgactccgcg gccgcagtgc gcgcgatgaa catccccgag ggcgccttct cacacatatc caccggcggc ggtgcctcgc tggaatacct tgagggcaag acgcttcccg gcatcgaggt actgagccgt gagcagccaa ccggaggagt tttgtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVANLKDL LAEGVSGRGV LVRSDLNVPL DEDGTITDAG RIIASAPTLK ALLDADAKVV VAAHLGRPKD GPDPTLSLAP VAVALGEQLG RHVQLAGDVV GADALARAEG LTGGDILLLE NIRFDKRETS KNDDDRRALA KQLVELVGTG GVFVSDGFGV VHRKQASVYD IATLLPHYAG TLVADEMRVL EQLTSSTQRP YAVVLGGSKV SDKLGVIESL ATKADSIVIG GGMCFTFLAA QGFSVGTSLL EDDMIEVCRG LLETYHDVLR LPVDLVVTEK FAADSPPQTV DVGAVPNGLM GLDIGPGSIK RFSTLLSNAG TIFWNGPMGV FEFPAYAAGT RGVAEAIVAA TGKGAFSVVG GGDSAAAVRA MNIPEGAFSH ISTGGGASLE YLEGKTLPGI EVLSREQPTG GVL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGTTGCA AACCTCAAGG ATCTACTCGC CGAAGGTGTT TCGGGGCGTG GAGTGCTGGT GCGCTCCGAT CTCAACGTTC CGCTCGACGA GGACGGCACC ATTACCGATG CGGGCCGCAT CATCGCGTCG GCGCCGACGT TGAAGGCGTT GCTCGACGCC GACGCCAAGG TGGTGGTTGC CGCGCACTTG GGACGTCCCA AGGACGGGCC GGACCCGACA CTGTCGCTGG CGCCGGTCGC CGTGGCGCTG GGTGAGCAAC TCGGCCGGCA CGTCCAGCTG GCTGGAGACG TTGTCGGCGC CGATGCGCTG GCCCGCGCCG AGGGGCTCAC CGGCGGCGAC ATCCTGCTGC TGGAGAACAT CCGCTTCGAC AAACGCGAAA CCAGCAAGAA CGATGACGAC CGGCGGGCAC TGGCCAAGCA GCTGGTCGAA CTGGTCGGAA CGGGAGGCGT TTTCGTCTCC GACGGCTTTG GGGTGGTGCA CCGCAAGCAA GCCTCGGTCT ATGACATCGC AACCCTGTTG CCGCACTACG CCGGCACGCT GGTCGCCGAC GAGATGCGGG TACTGGAGCA GTTGACCAGC TCGACCCAGC GGCCCTATGC GGTAGTGCTC GGCGGATCAA AGGTGTCCGA CAAGCTGGGT GTCATCGAGT CGCTGGCGAC CAAGGCGGAC AGCATTGTGA TTGGCGGCGG AATGTGCTTC ACATTCCTTG CTGCACAGGG ATTTTCGGTT GGCACATCGC TGCTGGAAGA CGACATGATC GAAGTCTGTC GCGGGCTGCT GGAAACCTAT CACGACGTGT TGCGGCTGCC CGTGGATCTA GTGGTCACGG AGAAGTTCGC CGCCGACTCG CCGCCCCAGA CGGTCGACGT CGGCGCTGTG CCCAATGGCT TGATGGGCCT GGATATCGGG CCGGGATCGA TCAAACGGTT CAGCACGCTG CTGTCCAACG CCGGGACCAT CTTCTGGAAC GGGCCGATGG GAGTATTCGA GTTCCCGGCT TATGCGGCCG GCACCAGAGG CGTCGCCGAG GCGATCGTCG CCGCCACCGG CAAAGGGGCG TTTAGTGTGG TCGGCGGCGG TGACTCCGCG GCCGCAGTGC GCGCGATGAA CATCCCCGAG GGCGCCTTCT CACACATATC CACCGGCGGC GGTGCCTCGC TGGAATACCT TGAGGGCAAG ACGCTTCCCG GCATCGAGGT ACTGAGCCGT GAGCAGCCAA CCGGAGGAGT TTTGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA