SSGCID: Target Page

MymaA.01331.a

Phosphoglycerate kinase

CENTER ID:	MymaA.01331.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01331.a.A1.GE30171	Full length( MymaA.01331.a )		1	416

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01331.a.A1.PW30044	Full length( MymaA.01331.a )		1	416

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.2392
RefSeq:	YP_001850544.1
UniProt:	B2HP85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTIHSLEDLL AEGVSGRGVL VRSDLNVPLD ENGVITDAGR ITASVPTLKA LLDGGAKVVV AAHLGRPKDG PDPKLSLEPV AAALGEQLGR HVQLAGDIVG TDALARAEGL TDGDVLLLEN IRFDKRETSK DDGERLALAK QLAELVSPGG AFVSDGFGVV HRKQASVYDV ATLLPHYAGR LVADEIRVLE QLTSSTERPY AVVLGGSKVS DKLGVIESLA TKADSIVIGG GMCFTFLAAQ GYSVGTSLLE KEMIDTCRRL LDTYHDVLRL PVDVVVTEKF AADSPPQTVA ADAIPADTMG LDIGPGSVKR FAALLSNAKT IFWNGPMGVF EFPAYATGTR GIAEAIVAAT GKGAFSVVGG GDSAAAVRAL GISEGSFSHI STGGGASLEY LEGKTLPGIE VLGRPQPGQT EGGGPA

NT Sequence

GTGACCATCC ATTCCCTTGA AGACCTTCTC GCTGAAGGTG TTTCGGGTCG CGGGGTGCTG GTGCGCTCCG ATCTGAATGT GCCGCTCGAC GAGAACGGTG TCATTACCGA CGCGGGCCGT ATCACCGCGT CGGTACCGAC CTTGAAGGCG CTGCTCGACG GCGGCGCCAA GGTCGTCGTC GCAGCGCATT TGGGTCGTCC CAAGGATGGG CCCGACCCGA AGCTGTCTCT GGAGCCGGTT GCCGCGGCTC TTGGCGAGCA ACTGGGCCGG CACGTGCAGT TGGCCGGAGA CATCGTCGGC ACCGATGCGC TCGCTCGTGC CGAAGGGCTG ACCGACGGCG ACGTCCTGCT GCTGGAGAAC ATCCGCTTCG ACAAGCGCGA AACCAGCAAG GACGACGGCG AGCGCCTCGC TTTGGCCAAG CAGCTCGCCG AACTGGTCAG CCCGGGAGGT GCTTTCGTCT CGGATGGCTT TGGCGTGGTG CACCGCAAGC AAGCTTCGGT GTATGACGTC GCCACGTTGC TGCCGCACTA TGCCGGCAGG TTGGTGGCTG ATGAAATCCG CGTGCTCGAG CAGTTGACCA GTTCGACTGA GCGGCCGTAC GCGGTGGTGC TGGGCGGGTC GAAGGTATCC GACAAGCTCG GTGTCATTGA GTCGCTGGCA ACCAAGGCCG ACAGCATCGT GATCGGCGGC GGAATGTGTT TCACATTCCT TGCCGCCCAA GGTTACTCGG TCGGCACGTC GCTGCTGGAA AAAGAGATGA TCGACACCTG TCGAAGGCTG CTCGACACCT ACCACGATGT GCTGCGGCTA CCCGTCGACG TGGTGGTGAC CGAGAAGTTC GCCGCCGATT CACCACCTCA AACCGTTGCC GCGGATGCGA TCCCGGCCGA CACGATGGGC TTGGATATCG GGCCGGGATC GGTCAAGAGG TTCGCCGCGC TGCTGTCCAA CGCCAAGACC ATCTTCTGGA ATGGCCCGAT GGGCGTATTC GAATTCCCGG CGTACGCGAC GGGCACCAGG GGCATCGCCG AGGCGATTGT CGCGGCGACC GGCAAGGGTG CGTTCAGCGT GGTCGGCGGC GGTGACTCCG CCGCCGCGGT TCGCGCGCTT GGCATTTCGG AGGGATCGTT CTCGCACATT TCCACCGGCG GCGGCGCGTC GCTGGAATAC CTGGAGGGCA AGACGCTGCC CGGCATCGAG GTGCTCGGTA GACCCCAGCC CGGCCAAACC GAGGGCGGAG GACCCGCG

Details for MymaA.01331.a.A1.GE30171

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MVTIHSLEDL LAEGVSGRGV LVRSDLNVPL DENGVITDAG RITASVPTLK ALLDGGAKVV VAAHLGRPKD GPDPKLSLEP VAAALGEQLG RHVQLAGDIV GTDALARAEG LTDGDVLLLE NIRFDKRETS KDDGERLALA KQLAELVSPG GAFVSDGFGV VHRKQASVYD VATLLPHYAG RLVADEIRVL EQLTSSTERP YAVVLGGSKV SDKLGVIESL ATKADSIVIG GGMCFTFLAA QGYSVGTSLL EKEMIDTCRR LLDTYHDVLR LPVDVVVTEK FAADSPPQTV AADAIPADTM GLDIGPGSVK RFAALLSNAK TIFWNGPMGV FEFPAYATGT RGIAEAIVAA TGKGAFSVVG GGDSAAAVRA LGISEGSFSH ISTGGGASLE YLEGKTLPGI EVLGRPQPGQ TEGGGPA

Validated NT Sequence

acgcgggtcc tccgccctcg gtttggccgg gctggggtct accgagcacc tcgatgccgg gcagcgtctt gccctccagg tattccagcg acgcgccgcc gccggtggaa atgtgcgaga acgatccctc cgaaatgcca agcgcgcgaa ccgcggcggc ggagtcaccg ccgccgacca cgctgaacgc acccttgccg gtcgccgcga caatcgcctc ggcgatgccc ctggtgcccg tcgcgtacgc cgggaattcg aatacgccca tcgggccatt ccagaagatg gtcttggcgt tggacagcag cgcggcgaac ctcttgaccg atcccggccc gatatccaag cccatcgtgt cggccgggat cgcatccgcg gcaacggttt gaggtggtga atcggcggcg aacttctcgg tcaccaccac gtcgacgggt agccgcagca catcgtggta ggtgtcgagc agccttcgac aggtgtcgat catctctttt tccagcagcg acgtgccgac cgagtaacct tgggcggcaa ggaatgtgaa acacattccg ccgccgatca cgatgctgtc ggccttggtt gccagcgact caatgacacc gagcttgtcg gataccttcg acccgcccag caccaccgcg tacggccgct cagtcgaact ggtcaactgc tcgagcacgc ggatttcatc agccaccaac ctgccggcat agtgcggcag caacgtggcg acgtcataca ccgaagcttg cttgcggtgc accacgccaa agccatccga gacgaaagca cctcccgggc tgaccagttc ggcgagctgc ttggccaaag cgaggcgctc gccgtcgtcc ttgctggttt cgcgcttgtc gaagcggatg ttctccagca gcaggacgtc gccgtcggtc agcccttcgg cacgagcgag cgcatcggtg ccgacgatgt ctccggccaa ctgcacgtgc cggcccagtt gctcgccaag agccgcggca accggctcca gagacagctt cgggtcgggc ccatccttgg gacgacccaa atgcgctgcg acgacgacct tggcgccgcc gtcgagcagc gccttcaagg tcggtaccga cgcggtgata cggcccgcgt cggtaatgac accgttctcg tcgagcggca cattcagatc ggagcgcacc agcaccccgc gacccgaaac accttcagcg agaaggtctt caagggaatg gatggtcacc atcgaaccag gaccctgggt ctgagcttcc ag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTIHSLED LLAEGVSGRG VLVRSDLNVP LDENGVITDA GRITASVPTL KALLDGGAKV VVAAHLGRPK DGPDPKLSLE PVAAALGEQL GRHVQLAGDI VGTDALARAE GLTDGDVLLL ENIRFDKRET SKDDGERLAL AKQLAELVSP GGAFVSDGFG VVHRKQASVY DVATLLPHYA GRLVADEIRV LEQLTSSTER PYAVVLGGSK VSDKLGVIES LATKADSIVI GGGMCFTFLA AQGYSVGTSL LEKEMIDTCR RLLDTYHDVL RLPVDVVVTE KFAADSPPQT VAADAIPADT MGLDIGPGSV KRFAALLSNA KTIFWNGPMG VFEFPAYATG TRGIAEAIVA ATGKGAFSVV GGGDSAAAVR ALGISEGSFS HISTGGGASL EYLEGKTLPG IEVLGRPQPG QTEGGGPA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccatc cattcccttg aagaccttct cgctgaaggt gtttcgggtc gcggggtgct ggtgcgctcc gatctgaatg tgccgctcga cgagaacggt gtcattaccg acgcgggccg tatcaccgcg tcggtaccga ccttgaaggc gctgctcgac ggcggcgcca aggtcgtcgt cgcagcgcat ttgggtcgtc ccaaggatgg gcccgacccg aagctgtctc tggagccggt tgccgcggct cttggcgagc aactgggccg gcacgtgcag ttggccggag acatcgtcgg caccgatgcg ctcgctcgtg ccgaagggct gaccgacggc gacgtcctgc tgctggagaa catccgcttc gacaagcgcg aaaccagcaa ggacgacggc gagcgcctcg ctttggccaa gcagctcgcc gaactggtca gcccgggagg tgctttcgtc tcggatggct ttggcgtggt gcaccgcaag caagcttcgg tgtatgacgt cgccacgttg ctgccgcact atgccggcag gttggtggct gatgaaatcc gcgtgctcga gcagttgacc agttcgactg agcggccgta cgcggtggtg ctgggcgggt cgaaggtatc cgacaagctc ggtgtcattg agtcgctggc aaccaaggcc gacagcatcg tgatcggcgg cggaatgtgt ttcacattcc ttgccgccca aggttactcg gtcggcacgt cgctgctgga aaaagagatg atcgacacct gtcgaaggct gctcgacacc taccacgatg tgctgcggct acccgtcgac gtggtggtga ccgagaagtt cgccgccgat tcaccacctc aaaccgttgc cgcggatgcg atcccggccg acacgatggg cttggatatc gggccgggat cggtcaagag gttcgccgcg ctgctgtcca acgccaagac catcttctgg aatggcccga tgggcgtatt cgaattcccg gcgtacgcga cgggcaccag gggcatcgcc gaggcgattg tcgcggcgac cggcaagggt gcgttcagcg tggtcggcgg cggtgactcc gccgccgcgg ttcgcgcgct tggcatttcg gagggatcgt tctcgcacat ttccaccggc ggcggcgcgt cgctggaata cctggagggc aagacgctgc ccggcatcga ggtgctcggt agaccccagc ccggccaaac cgagggcgga ggacccgcga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MymaA.01331.a.A1.PW30044

PURIFICATION DATe:	10/25/2010
CONCENTRATION:	25.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCATC CATTCCCTTG AAGACCTTCT CGCTGAAGGT GTTTCGGGTC GCGGGGTGCT GGTGCGCTCC GATCTGAATG TGCCGCTCGA CGAGAACGGT GTCATTACCG ACGCGGGCCG TATCACCGCG TCGGTACCGA CCTTGAAGGC GCTGCTCGAC GGCGGCGCCA AGGTCGTCGT CGCAGCGCAT TTGGGTCGTC CCAAGGATGG GCCCGACCCG AAGCTGTCTC TGGAGCCGGT TGCCGCGGCT CTTGGCGAGC AACTGGGCCG GCACGTGCAG TTGGCCGGAG ACATCGTCGG CACCGATGCG CTCGCTCGTG CCGAAGGGCT GACCGACGGC GACGTCCTGC TGCTGGAGAA CATCCGCTTC GACAAGCGCG AAACCAGCAA GGACGACGGC GAGCGCCTCG CTTTGGCCAA GCAGCTCGCC GAACTGGTCA GCCCGGGAGG TGCTTTCGTC TCGGATGGCT TTGGCGTGGT GCACCGCAAG CAAGCTTCGG TGTATGACGT CGCCACGTTG CTGCCGCACT ATGCCGGCAG GTTGGTGGCT GATGAAATCC GCGTGCTCGA GCAGTTGACC AGTTCGACTG AGCGGCCGTA CGCGGTGGTG CTGGGCGGGT CGAAGGTATC CGACAAGCTC GGTGTCATTG AGTCGCTGGC AACCAAGGCC GACAGCATCG TGATCGGCGG CGGAATGTGT TTCACATTCC TTGCCGCCCA AGGTTACTCG GTCGGCACGT CGCTGCTGGA AAAAGAGATG ATCGACACCT GTCGAAGGCT GCTCGACACC TACCACGATG TGCTGCGGCT ACCCGTCGAC GTGGTGGTGA CCGAGAAGTT CGCCGCCGAT TCACCACCTC AAACCGTTGC CGCGGATGCG ATCCCGGCCG ACACGATGGG CTTGGATATC GGGCCGGGAT CGGTCAAGAG GTTCGCCGCG CTGCTGTCCA ACGCCAAGAC CATCTTCTGG AATGGCCCGA TGGGCGTATT CGAATTCCCG GCGTACGCGA CGGGCACCAG GGGCATCGCC GAGGCGATTG TCGCGGCGAC CGGCAAGGGT GCGTTCAGCG TGGTCGGCGG CGGTGACTCC GCCGCCGCGG TTCGCGCGCT TGGCATTTCG GAGGGATCGT TCTCGCACAT TTCCACCGGC GGCGGCGCGT CGCTGGAATA CCTGGAGGGC AAGACGCTGC CCGGCATCGA GGTGCTCGGT AGACCCCAGC CCGGCCAAAC CGAGGGCGGA GGACCCGCGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A