MyulA.01331.a

Phosphoglycerate kinase

CENTER ID: MyulA.01331.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01331.a.A1.GE29887 Full length( MyulA.01331.a ) 1 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2186
RefSeq: YP_905773.1
UniProt: A0PPN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIHSLEDLP AEGVSGRGVL VRSDLNVPLD ENGVITDAGR ITASVPTLKA LLDGGAKVVV AAHLGRPKDG PDPKLSLEPV AAALGEQLGR HVQLAGDIVG TDALARAEGL TDGDVLLLEN IRFDKRETSK DDGERLAFAK QLAELVSPEG AFVSDGFGVV HRKQASVYDV ATLLPHYAGR LVADEIRVLE QLTSSTERPY AVVLGGSKVS DKLGVIESLA TKADSIVIGG GMCFTFLAAQ GYSVGTSLLE KEMIDTCRRL LDTYHDVLRL PVDVVVTEKF AADPPPQTVA ADAIPADTMG LDIGPGSVKR FAALLSNAKT IFWNGPMGVF EFPAYATGTR GIAEAIVAAT GKGAFSVVGG GDSAAAVRAL GISEGSFSHI STGGGASLEY LEGKTLPGIE VLGRPQPGQT EGGGPA
NT Sequence
GTGACCATCC ATTCCCTTGA AGACCTTCCC GCTGAAGGTG TTTCGGGTCG CGGGGTGCTG GTGCGCTCCG ATCTGAATGT GCCGCTCGAC GAGAACGGTG TCATTACCGA CGCGGGCCGT ATCACCGCGT CGGTACCGAC GTTGAAGGCG CTGCTCGACG GCGGCGCCAA GGTCGTCGTC GCAGCGCATT TGGGTCGTCC CAAGGATGGG CCCGACCCGA AGCTGTCTCT GGAGCCGGTT GCCGCGGCTC TTGGCGAGCA ACTGGGCCGG CACGTGCAGT TGGCCGGAGA CATCGTCGGC ACCGATGCGC TCGCTCGTGC CGAAGGGCTG ACCGACGGCG ACGTCCTGCT GCTGGAGAAC ATCCGCTTCG ACAAGCGCGA AACCAGCAAG GACGACGGCG AGCGCCTCGC TTTCGCCAAG CAGCTCGCCG AACTGGTCAG CCCGGAAGGT GCTTTCGTCT CGGATGGCTT TGGCGTGGTG CACCGCAAGC AAGCTTCGGT GTATGACGTC GCCACGTTGC TGCCGCACTA TGCCGGCAGG CTGGTGGCTG ATGAAATCCG CGTGCTCGAG CAGTTGACCA GTTCGACCGA GCGGCCGTAC GCGGTGGTGC TGGGCGGGTC GAAGGTATCC GACAAGCTCG GTGTCATCGA GTCGCTGGCA ACCAAGGCCG ACAGCATCGT GATCGGCGGC GGAATGTGTT TCACATTCCT TGCCGCCCAA GGTTACTCGG TCGGCACGTC GCTGCTGGAA AAAGAGATGA TCGACACCTG TCGAAGGCTG CTCGACACCT ACCACGATGT GCTGCGGCTA CCCGTCGACG TGGTGGTGAC CGAGAAGTTC GCCGCCGATC CACCACCTCA AACCGTTGCC GCGGATGCGA TCCCGGCCGA CACGATGGGC TTGGATATCG GGCCGGGATC GGTCAAGAGG TTCGCCGCGC TGCTGTCCAA CGCCAAGACC ATCTTCTGGA ATGGTCCGAT GGGCGTATTC GAATTCCCGG CGTACGCGAC GGGCACCAGG GGCATCGCCG AGGCGATTGT CGCGGCGACC GGCAAGGGTG CGTTCAGCGT GGTCGGCGGC GGTGACTCCG CCGCCGCGGT TCGCGCGCTT GGCATTTCGG AGGGATCGTT CTCGCACATT TCCACCGGCG GCGGCGCGTC GCTGGAATAC CTGGAGGGCA AGACGCTGCC CGGCATCGAG GTGCTCGGTA GACCCCAGCC CGGCCAAACC GAGGGCGGAG GACCCGCG
Details for MyulA.01331.a.A1.GE29887
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIHSLED LPAEGVSXXG VLVRSDLNVP LDENGVITDA GRITASVPTL KALLDGGAKV VVAAHLGRPK DGPDPKLSLE PVAAALGEQL GRHVQLAGDI VGTDALARAE GLTDGDVLLL ENIRFDKRET SKDDGERLAF AKQLAELVSP EGAFVSDGFG VVHRKQASVY DVATLLPHYA GRLVADEIRV LXQLTSSTER PYXXVLGGSK VSXKLGVIEX XATXXXSIXI GXXMXFTFXA XXGXXAXXAA XXXXSXXXXX LXXXTXXXXT XXXXXXXXXX IXXXXXXXXX XXXXXGXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
cnannanatt ttgtttactt nnnnaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgacca tccattccct tgaagacctt cccgctgaag gtgtttcnnn nngcggggtg ctggtgcgct ccgatctgaa tgtgccgctc gacgagaacg gtgtcattac cgacgcgggc cgtatcaccg cgtcggtacc gacgttgaag gcgctgctcg acggcggcgc caaggtcgtc gtcgcagcgc atttgggtcg tcccaaggat gggcccgacc cgaagctgtc tctggagccg gttgccgcgg ctcttggcga gcaactgggc cggcacgtgc agttggccgg agacatcgtc ggcaccgatg cgctcgctcg tgccgaaggg ctgaccgacg gcgacgtcct gctgctggag aacatccgct tcgacaagcg cgaaaccagc aaggacgacg gcgagcgcct cgctttcgcc aagcagctcg ccgaactggt cagcccggaa ggtgctttcg tctcggatgg ctttggcgtg gtgcaccgca agcaagcttc ggtgtatgac gtcgccacgt tgctgccgca ctatgccggc aggctggtgg ctgatgaaat ccgcgtgctc nagcagttga ccagttcnac cgagcggccg tacncggngg tgctgggcgg gtcgaaggta tccganaagc tcggtgtcat cgagncnntg gcaaccaann ccnanagcat cntgatcggn nncnnaatgn gnttcacatt cnttgcnncc cnnggttann cggcnnncnn ngctgctnaa aananatnat cnnnncngnn nnnnntgctc nacncnncna cnannngnng ngnnacnnnn nacntnntgn nannnnnnnn tncnncnatc nnnnncnnnn nnnnnnnnnc nnnnnnannc nnncnnnnnn anggnnngnn nnnnngnnnn nnngnnnnnn nnnnnnnnnn nnnngncnnn nnnnnnntnn nnnnnnnnnn gnnnnnnnnn nnnnnnnnnn nnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIHSLED LPAEGVSGRG VLVRSDLNVP LDENGVITDA GRITASVPTL KALLDGGAKV VVAAHLGRPK DGPDPKLSLE PVAAALGEQL GRHVQLAGDI VGTDALARAE GLTDGDVLLL ENIRFDKRET SKDDGERLAF AKQLAELVSP EGAFVSDGFG VVHRKQASVY DVATLLPHYA GRLVADEIRV LEQLTSSTER PYAVVLGGSK VSDKLGVIES LATKADSIVI GGGMCFTFLA AQGYSVGTSL LEKEMIDTCR RLLDTYHDVL RLPVDVVVTE KFAADPPPQT VAADAIPADT MGLDIGPGSV KRFAALLSNA KTIFWNGPMG VFEFPAYATG TRGIAEAIVA ATGKGAFSVV GGGDSAAAVR ALGISEGSFS HISTGGGASL EYLEGKTLPG IEVLGRPQPG QTEGGGP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccatc cattcccttg aagaccttcc cgctgaaggt gtttcgggtc gcggggtgct ggtgcgctcc gatctgaatg tgccgctcga cgagaacggt gtcattaccg acgcgggccg tatcaccgcg tcggtaccga cgttgaaggc gctgctcgac ggcggcgcca aggtcgtcgt cgcagcgcat ttgggtcgtc ccaaggatgg gcccgacccg aagctgtctc tggagccggt tgccgcggct cttggcgagc aactgggccg gcacgtgcag ttggccggag acatcgtcgg caccgatgcg ctcgctcgtg ccgaagggct gaccgacggc gacgtcctgc tgctggagaa catccgcttc gacaagcgcg aaaccagcaa ggacgacggc gagcgcctcg ctttcgccaa gcagctcgcc gaactggtca gcccggaagg tgctttcgtc tcggatggct ttggcgtggt gcaccgcaag caagcttcgg tgtatgacgt cgccacgttg ctgccgcact atgccggcag gctggtggct gatgaaatcc gcgtgctcga gcagttgacc agttcgaccg agcggccgta cgcggtggtg ctgggcgggt cgaaggtatc cgacaagctc ggtgtcatcg agtcgctggc aaccaaggcc gacagcatcg tgatcggcgg cggaatgtgt ttcacattcc ttgccgccca aggttactcg gtcggcacgt cgctgctgga aaaagagatg atcgacacct gtcgaaggct gctcgacacc taccacgatg tgctgcggct acccgtcgac gtggtggtga ccgagaagtt cgccgccgat ccaccacctc aaaccgttgc cgcggatgcg atcccggccg acacgatggg cttggatatc gggccgggat cggtcaagag gttcgccgcg ctgctgtcca acgccaagac catcttctgg aatggtccga tgggcgtatt cgaattcccg gcgtacgcga cgggcaccag gggcatcgcc gaggcgattg tcgcggcgac cggcaagggt gcgttcagcg tggtcggcgg cggtgactcc gccgccgcgg ttcgcgcgct tggcatttcg gagggatcgt tctcgcacat ttccaccggc ggcggcgcgt cgctggaata cctggagggc aagacgctgc ccggcatcga ggtgctcggt agaccccagc ccggccaaac cgagggcgga ggacccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa