MyavA.01331.a
Phosphoglycerate kinase
| CENTER ID: | MyavA.01331.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01331.a.A1.GE29113 | Full length( MyavA.01331.a ) | 1 | 415 | ||
| MyavA.01331.a.AE1.GE43236 | Full length( MyavA.01331.a ) | 1 | 415 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.01331.a.A1.PW29018 | Full length( MyavA.01331.a ) | 1 | 415 | ||
| MyavA.01331.a.AE1.PW38721 | Full length( MyavA.01331.a ) | 1 | 415 |
Structures
7LA1
DEPOSITED: 1/5/2021
DETERMINATION: XRay
CLONE: MyavA.01331.a.AE1.GE43236
PROTEIN: MyavA.01331.a.AE1.PW38721
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|243243.7.peg.3268 |
| RefSeq: | YP_882522.1 |
| UniProt: | A0QHY4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAVHNLKDLL AEGVSGRGVL VRSDLNVPLD SDGEQGRITD PGRITASVPT LSALVEAGAK VVVAAHLGRP KNGPDPALSL APVAAALGEQ LGRHVQLASD VVGTDALARA EGLTDGDVLL LENIRFDARE TSKDDAERLA LARQLAELVG PTGAFVSDGF GVVHRKQASV YDVATLLPHY AGTLVAEEIA VLEQLTGSTK RPYAVVLGGS KVSDKLGVIE SLATKADSIV IGGGMCFTFL AAQGFSVGKS LLETEMVDTC RRLLDTYVDV LRLPVDIVAA DRFAADAAPQ TVPADAIPDD LMGLDIGPGS VKRFTALLSN AETIFWNGPM GVFEFPAFAA GTKGLAEAIA AATGKGAFSV VGGGDSAAAV RALGIPESGF SHISTGGGAS LEYLEGKALP GIEVLGRPQP TGGAA
NT Sequence
GTGGCTGTCC ACAATCTCAA AGACCTTCTC GCCGAAGGTG TTTCCGGACG CGGTGTGCTG GTGCGTTCGG ACCTGAACGT GCCGCTGGAC TCCGATGGTG AGCAAGGCCG GATCACCGAC CCCGGCCGGA TCACCGCGTC GGTGCCGACG TTGAGCGCAC TGGTCGAGGC GGGCGCCAAG GTGGTGGTCG CCGCGCACCT CGGGCGTCCC AAGAATGGGC CGGACCCGGC GTTGTCGCTG GCCCCGGTGG CCGCCGCGCT CGGCGAGCAG CTGGGCCGGC ACGTGCAGCT GGCCTCGGAC GTGGTGGGCA CCGACGCCCT GGCCCGCGCC GAGGGGTTGA CCGACGGCGA CGTCCTGCTG CTGGAAAACA TCCGGTTCGA CGCCCGCGAA ACGTCCAAGG ACGACGCCGA GCGGCTCGCG CTGGCCCGGC AGCTGGCCGA ATTGGTCGGT CCCACAGGCG CTTTCGTCTC CGACGGTTTC GGCGTGGTGC ACCGCAAGCA GGCCTCGGTG TACGACGTCG CCACGCTGCT GCCGCACTAC GCCGGCACGC TGGTGGCCGA GGAGATCGCC GTGCTGGAGC AGCTGACCGG CTCGACCAAA CGCCCCTACG CGGTGGTGCT CGGCGGGTCC AAGGTGTCCG ACAAGCTCGG CGTCATCGAG TCGCTGGCCA CCAAGGCCGA CAGCATCGTC ATCGGCGGCG GCATGTGCTT CACCTTTCTT GCTGCGCAAG GGTTTTCGGT TGGTAAGTCG CTGCTAGAAA CCGAGATGGT GGACACCTGC CGCCGGCTGC TGGACACCTA CGTCGACGTG CTGCGGCTGC CGGTGGACAT CGTGGCGGCC GACAGGTTCG CCGCCGACGC GGCGCCGCAG ACCGTGCCGG CCGACGCCAT CCCCGACGAC CTGATGGGCC TGGACATCGG GCCGGGATCG GTCAAGAGGT TCACCGCGCT GCTGTCCAAC GCCGAGACGA TCTTCTGGAA CGGCCCGATG GGCGTGTTCG AGTTCCCGGC GTTCGCCGCG GGCACCAAGG GCCTGGCCGA AGCGATCGCC GCGGCGACCG GCAAGGGCGC CTTCTCCGTG GTCGGCGGCG GCGACTCGGC GGCCGCGGTG CGCGCGCTGG GCATCCCGGA GAGCGGCTTC TCGCACATTT CCACCGGCGG CGGGGCGTCG CTGGAATACC TTGAGGGCAA GGCACTTCCG GGCATCGAGG TGCTGGGACG TCCGCAACCG ACGGGAGGCG CCGCG
Details for MyavA.01331.a.A1.GE29113
| HARVESTED ON: | 4/27/2010 |
| SEQUENCED ON: | 5/1/2010 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MVAVHNLKDL LAEGVSGRGV LVRSDLNVPL DSDGEQGRIT DPGRITASVP TLSALVEAGA KVVVAAHLGR PKNGPDPALS LAPVAAALGE QLGRHVQLAS DVVGTDALAR AEGLTDGDVL LLENIRFDAR ETSKDDAERL ALARQLAELV GPTGAFVSDG FGVVHRKQAS VYDVATLLPH YAGTLVAEEI AVLEQLTGST KRPYAVVLGG SKVSDKLGVI ESLATKADSI VIGGGMCFTF LAAQGFSVGK SLLETEMVDT CRRLLDTYVD VLRLPVDIVA ADRFAADAAP QTVPADAIPD DLMGLDIGPG SVKRFTALLS NAETIFWNGP MGVFEFPAFA AGTKGLAEAI AAATGKGAFS VVGGGDSAAA VRALGIPESG FSHISTGGGA SLEYLEGKAL PGIEVLGRPQ PTGGAA
Validated NT Sequence
ttattacgcg gcgcctcccg tcggttgcgg acgtcccagc acctcgatgc ccggaagtgc cttgccctca aggtattcca gcgacgcccc gccgccggtg gaaatgtgcg agaagccgct ctccgggatg cccagcgcgc gcaccgcggc cgccgagtcg ccgccgccga ccacggagaa ggcgcccttg ccggtcgccg cggcgatcgc ttcggccagg cccttggtgc ccgcggcgaa cgccgggaac tcgaacacgc ccatcgggcc gttccagaag atcgtctcgg cgttggacag cagcgcggtg aacctcttga ccgatcccgg cccgatgtcc aggcccatca ggtcgtcggg gatggcgtcg gccggcacgg tctgcggcgc cgcgtcggcg gcgaacctgt cggccgccac gatgtccacc ggcagccgca gcacgtcgac gtaggtgtcc agcagccggc ggcaggtgtc caccatctcg gtttctagca gcgacttacc aaccgaaaac ccttgcgcag caagaaaggt gaagcacatg ccgccgccga tgacgatgct gtcggccttg gtggccagcg actcgatgac gccgagcttg tcggacacct tggacccgcc gagcaccacc gcgtaggggc gtttggtcga gccggtcagc tgctccagca cggcgatctc ctcggccacc agcgtgccgg cgtagtgcgg cagcagcgtg gcgacgtcgt acaccgaggc ctgcttgcgg tgcaccacgc cgaaaccgtc ggagacgaaa gcgcctgtgg gaccgaccaa ttcggccagc tgccgggcca gcgcgagccg ctcggcgtcg tccttggacg tttcgcgggc gtcgaaccgg atgttttcca gcagcaggac gtcgccgtcg gtcaacccct cggcgcgggc cagggcgtcg gtgcccacca cgtccgaggc cagctgcacg tgccggccca gctgctcgcc gagcgcggcg gccaccgggg ccagcgacaa cgccgggtcc ggcccattct tgggacgccc gaggtgcgcg gcgaccacca ccttggcgcc cgcctcgacc agtgcgctca acgtcggcac cgacgcggtg atccggccgg ggtcggtgat ccggccttgc tcaccatcgg agtccagcgg cacgttcagg tccgaacgca ccagcacacc gcgtccggaa acaccttcgg cgagaaggtc tttgagattg tggacagcca ccatcgaacc aggaccctgg gtctgagctt ccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAVHNLKD LLAEGVSGRG VLVRSDLNVP LDSDGEQGRI TDPGRITASV PTLSALVEAG AKVVVAAHLG RPKNGPDPAL SLAPVAAALG EQLGRHVQLA SDVVGTDALA RAEGLTDGDV LLLENIRFDA RETSKDDAER LALARQLAEL VGPTGAFVSD GFGVVHRKQA SVYDVATLLP HYAGTLVAEE IAVLEQLTGS TKRPYAVVLG GSKVSDKLGV IESLATKADS IVIGGGMCFT FLAAQGFSVG KSLLETEMVD TCRRLLDTYV DVLRLPVDIV AADRFAADAA PQTVPADAIP DDLMGLDIGP GSVKRFTALL SNAETIFWNG PMGVFEFPAF AAGTKGLAEA IAAATGKGAF SVVGGGDSAA AVRALGIPES GFSHISTGGG ASLEYLEGKA LPGIEVLGRP QPTGGAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctgtc cacaatctca aagaccttct cgccgaaggt gtttccggac gcggtgtgct ggtgcgttcg gacctgaacg tgccgctgga ctccgatggt gagcaaggcc ggatcaccga ccccggccgg atcaccgcgt cggtgccgac gttgagcgca ctggtcgagg cgggcgccaa ggtggtggtc gccgcgcacc tcgggcgtcc caagaatggg ccggacccgg cgttgtcgct ggccccggtg gccgccgcgc tcggcgagca gctgggccgg cacgtgcagc tggcctcgga cgtggtgggc accgacgccc tggcccgcgc cgaggggttg accgacggcg acgtcctgct gctggaaaac atccggttcg acgcccgcga aacgtccaag gacgacgccg agcggctcgc gctggcccgg cagctggccg aattggtcgg tcccacaggc gctttcgtct ccgacggttt cggcgtggtg caccgcaagc aggcctcggt gtacgacgtc gccacgctgc tgccgcacta cgccggcacg ctggtggccg aggagatcgc cgtgctggag cagctgaccg gctcgaccaa acgcccctac gcggtggtgc tcggcgggtc caaggtgtcc gacaagctcg gcgtcatcga gtcgctggcc accaaggccg acagcatcgt catcggcggc ggcatgtgct tcacctttct tgctgcgcaa gggttttcgg ttggtaagtc gctgctagaa accgagatgg tggacacctg ccgccggctg ctggacacct acgtcgacgt gctgcggctg ccggtggaca tcgtggcggc cgacaggttc gccgccgacg cggcgccgca gaccgtgccg gccgacgcca tccccgacga cctgatgggc ctggacatcg ggccgggatc ggtcaagagg ttcaccgcgc tgctgtccaa cgccgagacg atcttctgga acggcccgat gggcgtgttc gagttcccgg cgttcgccgc gggcaccaag ggcctggccg aagcgatcgc cgcggcgacc ggcaagggcg ccttctccgt ggtcggcggc ggcgactcgg cggccgcggt gcgcgcgctg ggcatcccgg agagcggctt ctcgcacatt tccaccggcg gcggggcgtc gctggaatac cttgagggca aggcacttcc gggcatcgag gtgctgggac gtccgcaacc gacgggaggc gccgcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyavA.01331.a.AE1.GE43236
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 44 |
Validated AA Sequence
MCFTFLAAQG FSVGKSLLET EMVDTCRRLL DTYVDVLRLP VDIVAADRFA ADAAPQTVPA DAIPDDLMGL DIGPGSVKRF TALLSNAETI FWNGPMGVFE FPAFAAGTKG LAEAIAAATG KGAFSVVGGG DSAAAVRALG IPESGFSHIS TGGGASLEYL EGKALPGIEV LGRPQPTGGA AAGHHHHHH
Validated NT Sequence
ctttcnnncg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg gagctcgaat tcggatccgt taatgatgat gatggtggtg cccagccgcg gcgcctcccg tcggttgcgg acgtcccagc acctcgatgc ccggaagtgc cttgccctca aggtattcca gcgacgcccc gccgccggtg gaaatgtgcg agaagccgct ctccgggatg cccagcgcgc gcaccgcggc cgccgagtcg ccgccgccga ccacggagaa ggcgcccttg ccggtcgccg ccgcgatcgc ttcggccagg cccttagtgc ccgcggcgaa cgccgggaac tcgaacacgc ccatcgggcc gttccagaag atcgtctcgg cgttggacag cagcgcggtg aacctcttga ccgatcccgg cccgatgtcc aggcccatca ggtcgtcggg gatggcgtcg gccggcacgg tctgcggcgc cgcgtcggcg gcgaacctgt cggccgccac aatgtccacc ggcagccgca gcacgtcgac gtaggtgtcc agcagccggc ggcaggtgtc caccatctcg gtttctagca gcgacttacc aaccgaaaac ccttgcgcag caagaaaggt gaagcacatg ccgccgccga tgacgatgct gtcggccttg gtggccagcg actcgatgac gccgagcttg tcggacacct tggacccgcc gagcaccacc gcgtangggc gtttggtcga gccggtcagc tgctccagca cggcgatctc ctcggccacc agcgtgccgg cgtantgcgg cagcagcgtg gcgacgtcgt acaccgaggc ctgcttgcgg gtgcaccacg ccgaaaccgt cgganacnaa agcgcctgtg gggancgacc aattcngcca gctgccgggg cagcgcgagc cgctcggcgt tcnnccttgg acgtttcngg gcgtcgaanc ngnatnnttt tccagcanna ggacgtcncc ntcggncaan cccctcggng nnng
Expected Protein Sequence
MAVHNLKDLL AEGVSGRGVL VRSDLNVPLD SDGEQGRITD PGRITASVPT LSALVEAGAK VVVAAHLGRP KNGPDPALSL APVAAALGEQ LGRHVQLASD VVGTDALARA EGLTDGDVLL LENIRFDARE TSKDDAERLA LARQLAELVG PTGAFVSDGF GVVHRKQASV YDVATLLPHY AGTLVAEEIA VLEQLTGSTK RPYAVVLGGS KVSDKLGVIE SLATKADSIV IGGGMCFTFL AAQGFSVGKS LLETEMVDTC RRLLDTYVDV LRLPVDIVAA DRFAADAAPQ TVPADAIPDD LMGLDIGPGS VKRFTALLSN AETIFWNGPM GVFEFPAFAA GTKGLAEAIA AATGKGAFSV VGGGDSAAAV RALGIPESGF SHISTGGGAS LEYLEGKALP GIEVLGRPQP TGGAAGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt ggctgtccac aatctcaaag accttctcgc cgaaggtgtt tccggacgcg gtgtgctggt gcgttcggac ctgaacgtgc cgctggactc cgatggtgag caaggccgga tcaccgaccc cggccggatc accgcgtcgg tgccgacgtt gagcgcactg gtcgaggcgg gcgccaaggt ggtggtcgcc gcgcacctcg ggcgtcccaa gaatgggccg gacccggcgt tgtcgctggc cccggtggcc gccgcgctcg gcgagcagct gggccggcac gtgcagctgg cctcggacgt ggtgggcacc gacgccctgg cccgcgccga ggggttgacc gacggcgacg tcctgctgct ggaaaacatc cggttcgacg cccgcgaaac gtccaaggac gacgccgagc ggctcgcgct ggcccggcag ctggccgaat tggtcggtcc cacaggcgct ttcgtctccg acggtttcgg cgtggtgcac cgcaagcagg cctcggtgta cgacgtcgcc acgctgctgc cgcactacgc cggcacgctg gtggccgagg agatcgccgt gctggagcag ctgaccggct cgaccaaacg cccctacgcg gtggtgctcg gcgggtccaa ggtgtccgac aagctcggcg tcatcgagtc gctggccacc aaggccgaca gcatcgtcat cggcggcggc atgtgcttca cctttcttgc tgcgcaaggg ttttcggttg gtaagtcgct gctagaaacc gagatggtgg acacctgccg ccggctgctg gacacctacg tcgacgtgct gcggctgccg gtggacatcg tggcggccga caggttcgcc gccgacgcgg cgccgcagac cgtgccggcc gacgccatcc ccgacgacct gatgggcctg gacatcgggc cgggatcggt caagaggttc accgcgctgc tgtccaacgc cgagacgatc ttctggaacg gcccgatggg cgtgttcgag ttcccggcgt tcgccgcggg caccaagggc ctggccgaag cgatcgccgc ggcgaccggc aagggcgcct tctccgtggt cggcggcggc gactcggcgg ccgcggtgcg cgcgctgggc atcccggaga gcggcttctc gcacatttcc accggcggcg gggcgtcgct ggaatacctt gagggcaagg cacttccggg catcgaggtg ctgggacgtc cgcaaccgac gggaggcgcc gcggggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for MyavA.01331.a.A1.PW29018
| PURIFICATION DATe: | 7/13/2010 |
| CONCENTRATION: | 26.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVAVHNLKDL LAEGVSGRGV LVRSDLNVPL DSDGEQGRIT DPGRITASVP TLSALVEAGA KVVVAAHLGR PKNGPDPALS LAPVAAALGE QLGRHVQLAS DVVGTDALAR AEGLTDGDVL LLENIRFDAR ETSKDDAERL ALARQLAELV GPTGAFVSDG FGVVHRKQAS VYDVATLLPH YAGTLVAEEI AVLEQLTGST KRPYAVVLGG SKVSDKLGVI ESLATKADSI VIGGGMCFTF LAAQGFSVGK SLLETEMVDT CRRLLDTYVD VLRLPVDIVA ADRFAADAAP QTVPADAIPD DLMGLDIGPG SVKRFTALLS NAETIFWNGP MGVFEFPAFA AGTKGLAEAI AAATGKGAFS VVGGGDSAAA VRALGIPESG FSHISTGGGA SLEYLEGKAL PGIEVLGRPQ PTGGAA
Validated NT Sequence
ttattacgcg gcgcctcccg tcggttgcgg acgtcccagc acctcgatgc ccggaagtgc cttgccctca aggtattcca gcgacgcccc gccgccggtg gaaatgtgcg agaagccgct ctccgggatg cccagcgcgc gcaccgcggc cgccgagtcg ccgccgccga ccacggagaa ggcgcccttg ccggtcgccg cggcgatcgc ttcggccagg cccttggtgc ccgcggcgaa cgccgggaac tcgaacacgc ccatcgggcc gttccagaag atcgtctcgg cgttggacag cagcgcggtg aacctcttga ccgatcccgg cccgatgtcc aggcccatca ggtcgtcggg gatggcgtcg gccggcacgg tctgcggcgc cgcgtcggcg gcgaacctgt cggccgccac gatgtccacc ggcagccgca gcacgtcgac gtaggtgtcc agcagccggc ggcaggtgtc caccatctcg gtttctagca gcgacttacc aaccgaaaac ccttgcgcag caagaaaggt gaagcacatg ccgccgccga tgacgatgct gtcggccttg gtggccagcg actcgatgac gccgagcttg tcggacacct tggacccgcc gagcaccacc gcgtaggggc gtttggtcga gccggtcagc tgctccagca cggcgatctc ctcggccacc agcgtgccgg cgtagtgcgg cagcagcgtg gcgacgtcgt acaccgaggc ctgcttgcgg tgcaccacgc cgaaaccgtc ggagacgaaa gcgcctgtgg gaccgaccaa ttcggccagc tgccgggcca gcgcgagccg ctcggcgtcg tccttggacg tttcgcgggc gtcgaaccgg atgttttcca gcagcaggac gtcgccgtcg gtcaacccct cggcgcgggc cagggcgtcg gtgcccacca cgtccgaggc cagctgcacg tgccggccca gctgctcgcc gagcgcggcg gccaccgggg ccagcgacaa cgccgggtcc ggcccattct tgggacgccc gaggtgcgcg gcgaccacca ccttggcgcc cgcctcgacc agtgcgctca acgtcggcac cgacgcggtg atccggccgg ggtcggtgat ccggccttgc tcaccatcgg agtccagcgg cacgttcagg tccgaacgca ccagcacacc gcgtccggaa acaccttcgg cgagaaggtc tttgagattg tggacagcca ccatcgaacc aggaccctgg gtctgagctt ccagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAVHNLKD LLAEGVSGRG VLVRSDLNVP LDSDGEQGRI TDPGRITASV PTLSALVEAG AKVVVAAHLG RPKNGPDPAL SLAPVAAALG EQLGRHVQLA SDVVGTDALA RAEGLTDGDV LLLENIRFDA RETSKDDAER LALARQLAEL VGPTGAFVSD GFGVVHRKQA SVYDVATLLP HYAGTLVAEE IAVLEQLTGS TKRPYAVVLG GSKVSDKLGV IESLATKADS IVIGGGMCFT FLAAQGFSVG KSLLETEMVD TCRRLLDTYV DVLRLPVDIV AADRFAADAA PQTVPADAIP DDLMGLDIGP GSVKRFTALL SNAETIFWNG PMGVFEFPAF AAGTKGLAEA IAAATGKGAF SVVGGGDSAA AVRALGIPES GFSHISTGGG ASLEYLEGKA LPGIEVLGRP QPTGGAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCTGTC CACAATCTCA AAGACCTTCT CGCCGAAGGT GTTTCCGGAC GCGGTGTGCT GGTGCGTTCG GACCTGAACG TGCCGCTGGA CTCCGATGGT GAGCAAGGCC GGATCACCGA CCCCGGCCGG ATCACCGCGT CGGTGCCGAC GTTGAGCGCA CTGGTCGAGG CGGGCGCCAA GGTGGTGGTC GCCGCGCACC TCGGGCGTCC CAAGAATGGG CCGGACCCGG CGTTGTCGCT GGCCCCGGTG GCCGCCGCGC TCGGCGAGCA GCTGGGCCGG CACGTGCAGC TGGCCTCGGA CGTGGTGGGC ACCGACGCCC TGGCCCGCGC CGAGGGGTTG ACCGACGGCG ACGTCCTGCT GCTGGAAAAC ATCCGGTTCG ACGCCCGCGA AACGTCCAAG GACGACGCCG AGCGGCTCGC GCTGGCCCGG CAGCTGGCCG AATTGGTCGG TCCCACAGGC GCTTTCGTCT CCGACGGTTT CGGCGTGGTG CACCGCAAGC AGGCCTCGGT GTACGACGTC GCCACGCTGC TGCCGCACTA CGCCGGCACG CTGGTGGCCG AGGAGATCGC CGTGCTGGAG CAGCTGACCG GCTCGACCAA ACGCCCCTAC GCGGTGGTGC TCGGCGGGTC CAAGGTGTCC GACAAGCTCG GCGTCATCGA GTCGCTGGCC ACCAAGGCCG ACAGCATCGT CATCGGCGGC GGCATGTGCT TCACCTTTCT TGCTGCGCAA GGGTTTTCGG TTGGTAAGTC GCTGCTAGAA ACCGAGATGG TGGACACCTG CCGCCGGCTG CTGGACACCT ACGTCGACGT GCTGCGGCTG CCGGTGGACA TCGTGGCGGC CGACAGGTTC GCCGCCGACG CGGCGCCGCA GACCGTGCCG GCCGACGCCA TCCCCGACGA CCTGATGGGC CTGGACATCG GGCCGGGATC GGTCAAGAGG TTCACCGCGC TGCTGTCCAA CGCCGAGACG ATCTTCTGGA ACGGCCCGAT GGGCGTGTTC GAGTTCCCGG CGTTCGCCGC GGGCACCAAG GGCCTGGCCG AAGCGATCGC CGCGGCGACC GGCAAGGGCG CCTTCTCCGT GGTCGGCGGC GGCGACTCGG CGGCCGCGGT GCGCGCGCTG GGCATCCCGG AGAGCGGCTT CTCGCACATT TCCACCGGCG GCGGGGCGTC GCTGGAATAC CTTGAGGGCA AGGCACTTCC GGGCATCGAG GTGCTGGGAC GTCCGCAACC GACGGGAGGC GCCGCGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for MyavA.01331.a.AE1.PW38721
| PURIFICATION DATe: | 10/17/2019 |
| CONCENTRATION: | 8.6mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 44 |
Protocol Notes
notes unavailable
Validated AA Sequence
MCFTFLAAQG FSVGKSLLET EMVDTCRRLL DTYVDVLRLP VDIVAADRFA ADAAPQTVPA DAIPDDLMGL DIGPGSVKRF TALLSNAETI FWNGPMGVFE FPAFAAGTKG LAEAIAAATG KGAFSVVGGG DSAAAVRALG IPESGFSHIS TGGGASLEYL EGKALPGIEV LGRPQPTGGA AAGHHHHHH
Validated NT Sequence
ctttcnnncg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg gagctcgaat tcggatccgt taatgatgat gatggtggtg cccagccgcg gcgcctcccg tcggttgcgg acgtcccagc acctcgatgc ccggaagtgc cttgccctca aggtattcca gcgacgcccc gccgccggtg gaaatgtgcg agaagccgct ctccgggatg cccagcgcgc gcaccgcggc cgccgagtcg ccgccgccga ccacggagaa ggcgcccttg ccggtcgccg ccgcgatcgc ttcggccagg cccttagtgc ccgcggcgaa cgccgggaac tcgaacacgc ccatcgggcc gttccagaag atcgtctcgg cgttggacag cagcgcggtg aacctcttga ccgatcccgg cccgatgtcc aggcccatca ggtcgtcggg gatggcgtcg gccggcacgg tctgcggcgc cgcgtcggcg gcgaacctgt cggccgccac aatgtccacc ggcagccgca gcacgtcgac gtaggtgtcc agcagccggc ggcaggtgtc caccatctcg gtttctagca gcgacttacc aaccgaaaac ccttgcgcag caagaaaggt gaagcacatg ccgccgccga tgacgatgct gtcggccttg gtggccagcg actcgatgac gccgagcttg tcggacacct tggacccgcc gagcaccacc gcgtangggc gtttggtcga gccggtcagc tgctccagca cggcgatctc ctcggccacc agcgtgccgg cgtantgcgg cagcagcgtg gcgacgtcgt acaccgaggc ctgcttgcgg gtgcaccacg ccgaaaccgt cgganacnaa agcgcctgtg gggancgacc aattcngcca gctgccgggg cagcgcgagc cgctcggcgt tcnnccttgg acgtttcngg gcgtcgaanc ngnatnnttt tccagcanna ggacgtcncc ntcggncaan cccctcggng nnng
Expressed Protein Sequence
MAVHNLKDLL AEGVSGRGVL VRSDLNVPLD SDGEQGRITD PGRITASVPT LSALVEAGAK VVVAAHLGRP KNGPDPALSL APVAAALGEQ LGRHVQLASD VVGTDALARA EGLTDGDVLL LENIRFDARE TSKDDAERLA LARQLAELVG PTGAFVSDGF GVVHRKQASV YDVATLLPHY AGTLVAEEIA VLEQLTGSTK RPYAVVLGGS KVSDKLGVIE SLATKADSIV IGGGMCFTFL AAQGFSVGKS LLETEMVDTC RRLLDTYVDV LRLPVDIVAA DRFAADAAPQ TVPADAIPDD LMGLDIGPGS VKRFTALLSN AETIFWNGPM GVFEFPAFAA GTKGLAEAIA AATGKGAFSV VGGGDSAAAV RALGIPESGF SHISTGGGAS LEYLEGKALP GIEVLGRPQP TGGAAGHHHH HH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCGT GGCTGTCCAC AATCTCAAAG ACCTTCTCGC CGAAGGTGTT TCCGGACGCG GTGTGCTGGT GCGTTCGGAC CTGAACGTGC CGCTGGACTC CGATGGTGAG CAAGGCCGGA TCACCGACCC CGGCCGGATC ACCGCGTCGG TGCCGACGTT GAGCGCACTG GTCGAGGCGG GCGCCAAGGT GGTGGTCGCC GCGCACCTCG GGCGTCCCAA GAATGGGCCG GACCCGGCGT TGTCGCTGGC CCCGGTGGCC GCCGCGCTCG GCGAGCAGCT GGGCCGGCAC GTGCAGCTGG CCTCGGACGT GGTGGGCACC GACGCCCTGG CCCGCGCCGA GGGGTTGACC GACGGCGACG TCCTGCTGCT GGAAAACATC CGGTTCGACG CCCGCGAAAC GTCCAAGGAC GACGCCGAGC GGCTCGCGCT GGCCCGGCAG CTGGCCGAAT TGGTCGGTCC CACAGGCGCT TTCGTCTCCG ACGGTTTCGG CGTGGTGCAC CGCAAGCAGG CCTCGGTGTA CGACGTCGCC ACGCTGCTGC CGCACTACGC CGGCACGCTG GTGGCCGAGG AGATCGCCGT GCTGGAGCAG CTGACCGGCT CGACCAAACG CCCCTACGCG GTGGTGCTCG GCGGGTCCAA GGTGTCCGAC AAGCTCGGCG TCATCGAGTC GCTGGCCACC AAGGCCGACA GCATCGTCAT CGGCGGCGGC ATGTGCTTCA CCTTTCTTGC TGCGCAAGGG TTTTCGGTTG GTAAGTCGCT GCTAGAAACC GAGATGGTGG ACACCTGCCG CCGGCTGCTG GACACCTACG TCGACGTGCT GCGGCTGCCG GTGGACATCG TGGCGGCCGA CAGGTTCGCC GCCGACGCGG CGCCGCAGAC CGTGCCGGCC GACGCCATCC CCGACGACCT GATGGGCCTG GACATCGGGC CGGGATCGGT CAAGAGGTTC ACCGCGCTGC TGTCCAACGC CGAGACGATC TTCTGGAACG GCCCGATGGG CGTGTTCGAG TTCCCGGCGT TCGCCGCGGG CACCAAGGGC CTGGCCGAAG CGATCGCCGC GGCGACCGGC AAGGGCGCCT TCTCCGTGGT CGGCGGCGGC GACTCGGCGG CCGCGGTGCG CGCGCTGGGC ATCCCGGAGA GCGGCTTCTC GCACATTTCC ACCGGCGGCG GGGCGTCGCT GGAATACCTT GAGGGCAAGG CACTTCCGGG CATCGAGGTG CTGGGACGTC CGCAACCGAC GGGAGGCGCC GCGGGGCACC ACCATCATCA TCATTAACGG ATCCGAATTC GAGCTCCGTC GACAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T