MysmA.01331.a

Phosphoglycerate kinase

CENTER ID: MysmA.01331.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01331.a.A1.GE28911 Full length( MysmA.01331.a ) 1 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01331.a.A1.PW28816 Full length( MysmA.01331.a ) 1 408

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3046
RefSeq: YP_887401.1
UniProt: A0QWW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVKTLDDLL AEGVQGRGVL VRSDLNVPLD DDGNITDPGR VIASVPTLQA LAEAGAKVIV TAHLGRPKGE PDPKLSLAPV AAALGEKLGR HVQLAGDVVG TDALARAEGL TDGDVLLLEN IRFDARETSK DDSERLSLAK ALAALVEGPD GSPGVFVSDG FGVVHRKQAS VYDVATLLPH YAGTLVAAEV KVLQQLTSST DRPYAVVLGG SKVSDKLAVI ENLATKADSL IIGGGMCFTF LAAQGFSVGS SLLQEEMVDT CRRLLDEYAD VIHLPVDIVV ADKFAADAEA ETVAADRIPD GKMGLDIGPG SVERFTALLS NAKTVFWNGP MGVFEFPAFA AGTKGVAEAI IGATGKGAFS VVGGGDSAAA VRRLGLPEDG FSHISTGGGA SLEYLEGKEL PGIQVLES
NT Sequence
ATGTCGGTCA AGACACTCGA CGATCTGCTC GCCGAGGGCG TGCAAGGTCG GGGCGTACTC GTGCGCTCCG ACCTCAACGT TCCCCTCGAC GACGACGGCA ACATCACCGA TCCGGGACGC GTCATCGCAT CGGTCCCGAC GCTGCAGGCC CTCGCGGAAG CGGGTGCCAA GGTGATCGTC ACCGCGCATC TCGGCCGCCC CAAGGGCGAG CCGGACCCCA AGCTGTCGCT CGCGCCGGTC GCGGCGGCAC TGGGGGAGAA GCTGGGCAGG CACGTGCAGC TCGCGGGTGA CGTCGTCGGC ACCGATGCGC TCGCGCGCGC CGAGGGCCTG ACCGACGGCG ACGTGCTGCT GCTGGAGAAC ATCCGGTTCG ACGCGCGCGA GACCAGCAAG GACGACAGCG AGCGCCTGTC GCTCGCCAAG GCCCTCGCGG CGCTCGTGGA AGGTCCCGAC GGATCACCCG GCGTCTTCGT CTCCGACGGT TTCGGTGTGG TGCACCGCAA GCAGGCGTCG GTCTACGACG TCGCGACGCT GCTGCCGCAC TACGCGGGCA CGCTGGTGGC CGCCGAGGTC AAGGTGCTGC AGCAGCTGAC CAGCTCGACG GACCGTCCGT ACGCCGTCGT GCTCGGCGGC TCGAAGGTGT CCGACAAGCT CGCGGTCATC GAGAACCTCG CGACGAAGGC CGACAGCCTG ATCATCGGTG GCGGTATGTG CTTCACATTC CTGGCCGCAC AAGGGTTTTC GGTCGGCAGC TCGCTGCTGC AGGAGGAGAT GGTCGACACC TGCCGCCGCC TGCTCGACGA GTACGCCGAT GTGATCCACC TGCCGGTGGA CATCGTCGTG GCCGACAAGT TCGCCGCCGA CGCGGAAGCC GAGACCGTTG CCGCCGACCG CATCCCGGAC GGCAAGATGG GCCTGGACAT CGGCCCGGGC TCGGTGGAGC GGTTCACCGC GCTGCTGAGC AACGCCAAGA CCGTGTTCTG GAACGGCCCG ATGGGTGTGT TCGAGTTCCC GGCGTTCGCG GCCGGCACCA AGGGTGTCGC CGAGGCGATC ATCGGCGCCA CCGGCAAGGG CGCGTTCAGC GTCGTTGGCG GCGGCGATTC CGCGGCCGCG GTACGCCGGC TCGGCCTGCC CGAGGACGGC TTCTCGCACA TCTCCACCGG TGGTGGCGCG TCGCTCGAAT ACCTCGAGGG CAAGGAATTG CCCGGCATCC AAGTACTGGA GTCG
Details for MysmA.01331.a.A1.GE28911
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/16/2012
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSVKTLDDLL AEGVQGRGVL VRSDLNVPLD DDGNITDPGR VIASVPTLQA LAEAGAKVIV TAHLGRPKGE PDPKLSLAPV AAALGEKLGR HVQLAGDVVG TDALARAEGL TDGDVLLLEN IRFDARETSK DDSERLSLAK ALAALVEGPD GSPGVFVSDG FGVVHRKQAS VYDVATLLPH YAGTLVAAEV KVLQQLTSST DRPYAVVLGG SKVSDKLAVI ENLATKADSL IIGGGMCFTF LAAQGFSVGS SLLQEEMVDT CRRLLDEYAD VIHLPVDIVV ADKFAADAEA ETVAADRIPD GKMGLDIGPG SVERFTALLS NAKTVFWNGP MGVFEFPAFA AGTKGVAEAI IGATGKGAFS VVGGGDSAAA VRRLGLPEDG FSHISTGGGA SLEYLEGKEL PGIQVL
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatgtcggt caagacactc gacgatctgc tcgccgaggg cgtgcaaggt cggggcgtac tcgtgcgctc cgacctcaac gttcccctcg acgacgacgg caacatcacc gatccgggac gcgtcatcgc atcggtcccg acgctgcagg ccctcgcgga agcgggtgcc aaggtgatcg tcaccgcgca tctcggccgc cccaagggcg agccggaccc caagctgtcg ctcgcgccgg tcgcggcggc actgggggag aagctgggca ggcacgtgca gctcgcgggt gacgtcgtcg gcaccgatgc gctcgcgcgc gccgagggcc tgaccgacgg cgacgtgctg ctgctggaga acatccggtt cgacgcgcgc gagaccagca aggacgacag cgagcgcctg tcgctcgcca aggccctcgc ggcgctcgtg gaaggtcccg acggatcacc cggcgtcttc gtctccgacg gtttcggtgt ggtgcaccgc aagcaggcgt cggtctacga cgtcgcgacg ctgctgccgc actacgcggg cacgctggtg gccgccgagg tcaaggtgct gcagcagctg accagctcga cggaccgtcc gtacgccgtc gtgctcggcg gctcgaaggt gtccgacaag ctcgcggtca tcgagaacct cgcgacgaag gccgacagcc tgatcatcgg tggcggtatg tgcttcacat tcctggccgc acaagggttt tcggtcggca gctcgctgct gcaggaggag atggtcgaca cctgccgccg cctgctcgac gagtacgccg atgtgatcca cctgccggtg gacatcgtcg tggccgacaa gttcgccgcc gacgcggaag ccgagaccgt tgccgccgac cgcatcccgg acggcaagat gggcctggac atcggcccgg gctcggtgga gcggttcacc gcgctgctga gcaacgccaa gaccgtgttc tggaacggcc cgatgggtgt gttcgagttc ccggcgttcg cggccggcac caagggtgtc gccgaggcga tcatcggcgc caccggcaag ggcgcgttca gcgtcgttgg cggcggcgat tccgcggccg cggtacgccg gctcggcctg cccgaggacg gcttctcgca catctccacc ggtggtggcg cgtcgctcga atacctcgag ggcaaggaat tgcccggcat ccaagtactg g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVKTLDDL LAEGVQGRGV LVRSDLNVPL DDDGNITDPG RVIASVPTLQ ALAEAGAKVI VTAHLGRPKG EPDPKLSLAP VAAALGEKLG RHVQLAGDVV GTDALARAEG LTDGDVLLLE NIRFDARETS KDDSERLSLA KALAALVEGP DGSPGVFVSD GFGVVHRKQA SVYDVATLLP HYAGTLVAAE VKVLQQLTSS TDRPYAVVLG GSKVSDKLAV IENLATKADS LIIGGGMCFT FLAAQGFSVG SSLLQEEMVD TCRRLLDEYA DVIHLPVDIV VADKFAADAE AETVAADRIP DGKMGLDIGP GSVERFTALL SNAKTVFWNG PMGVFEFPAF AAGTKGVAEA IIGATGKGAF SVVGGGDSAA AVRRLGLPED GFSHISTGGG ASLEYLEGKE LPGIQVLES
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcggtcaag acactcgacg atctgctcgc cgagggcgtg caaggtcggg gcgtactcgt gcgctccgac ctcaacgttc ccctcgacga cgacggcaac atcaccgatc cgggacgcgt catcgcatcg gtcccgacgc tgcaggccct cgcggaagcg ggtgccaagg tgatcgtcac cgcgcatctc ggccgcccca agggcgagcc ggaccccaag ctgtcgctcg cgccggtcgc ggcggcactg ggggagaagc tgggcaggca cgtgcagctc gcgggtgacg tcgtcggcac cgatgcgctc gcgcgcgccg agggcctgac cgacggcgac gtgctgctgc tggagaacat ccggttcgac gcgcgcgaga ccagcaagga cgacagcgag cgcctgtcgc tcgccaaggc cctcgcggcg ctcgtggaag gtcccgacgg atcacccggc gtcttcgtct ccgacggttt cggtgtggtg caccgcaagc aggcgtcggt ctacgacgtc gcgacgctgc tgccgcacta cgcgggcacg ctggtggccg ccgaggtcaa ggtgctgcag cagctgacca gctcgacgga ccgtccgtac gccgtcgtgc tcggcggctc gaaggtgtcc gacaagctcg cggtcatcga gaacctcgcg acgaaggccg acagcctgat catcggtggc ggtatgtgct tcacattcct ggccgcacaa gggttttcgg tcggcagctc gctgctgcag gaggagatgg tcgacacctg ccgccgcctg ctcgacgagt acgccgatgt gatccacctg ccggtggaca tcgtcgtggc cgacaagttc gccgccgacg cggaagccga gaccgttgcc gccgaccgca tcccggacgg caagatgggc ctggacatcg gcccgggctc ggtggagcgg ttcaccgcgc tgctgagcaa cgccaagacc gtgttctgga acggcccgat gggtgtgttc gagttcccgg cgttcgcggc cggcaccaag ggtgtcgccg aggcgatcat cggcgccacc ggcaagggcg cgttcagcgt cgttggcggc ggcgattccg cggccgcggt acgccggctc ggcctgcccg aggacggctt ctcgcacatc tccaccggtg gtggcgcgtc gctcgaatac ctcgagggca aggaattgcc cggcatccaa gtactggagt cgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MysmA.01331.a.A1.PW28816
PURIFICATION DATe: 6/7/2010
CONCENTRATION: 20.95mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MSVKTLDDLL AEGVQGRGVL VRSDLNVPLD DDGNITDPGR VIASVPTLQA LAEAGAKVIV TAHLGRPKGE PDPKLSLAPV AAALGEKLGR HVQLAGDVVG TDALARAEGL TDGDVLLLEN IRFDARETSK DDSERLSLAK ALAALVEGPD GSPGVFVSDG FGVVHRKQAS VYDVATLLPH YAGTLVAAEV KVLQQLTSST DRPYAVVLGG SKVSDKLAVI ENLATKADSL IIGGGMCFTF LAAQGFSVGS SLLQEEMVDT CRRLLDEYAD VIHLPVDIVV ADKFAADAEA ETVAADRIPD GKMGLDIGPG SVERFTALLS NAKTVFWNGP MGVFEFPAFA AGTKGVAEAI IGATGKGAFS VVGGGDSAAA VRRLGLPEDG FSHISTGGGA SLEYLEGKEL PGIQVL
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatgtcggt caagacactc gacgatctgc tcgccgaggg cgtgcaaggt cggggcgtac tcgtgcgctc cgacctcaac gttcccctcg acgacgacgg caacatcacc gatccgggac gcgtcatcgc atcggtcccg acgctgcagg ccctcgcgga agcgggtgcc aaggtgatcg tcaccgcgca tctcggccgc cccaagggcg agccggaccc caagctgtcg ctcgcgccgg tcgcggcggc actgggggag aagctgggca ggcacgtgca gctcgcgggt gacgtcgtcg gcaccgatgc gctcgcgcgc gccgagggcc tgaccgacgg cgacgtgctg ctgctggaga acatccggtt cgacgcgcgc gagaccagca aggacgacag cgagcgcctg tcgctcgcca aggccctcgc ggcgctcgtg gaaggtcccg acggatcacc cggcgtcttc gtctccgacg gtttcggtgt ggtgcaccgc aagcaggcgt cggtctacga cgtcgcgacg ctgctgccgc actacgcggg cacgctggtg gccgccgagg tcaaggtgct gcagcagctg accagctcga cggaccgtcc gtacgccgtc gtgctcggcg gctcgaaggt gtccgacaag ctcgcggtca tcgagaacct cgcgacgaag gccgacagcc tgatcatcgg tggcggtatg tgcttcacat tcctggccgc acaagggttt tcggtcggca gctcgctgct gcaggaggag atggtcgaca cctgccgccg cctgctcgac gagtacgccg atgtgatcca cctgccggtg gacatcgtcg tggccgacaa gttcgccgcc gacgcggaag ccgagaccgt tgccgccgac cgcatcccgg acggcaagat gggcctggac atcggcccgg gctcggtgga gcggttcacc gcgctgctga gcaacgccaa gaccgtgttc tggaacggcc cgatgggtgt gttcgagttc ccggcgttcg cggccggcac caagggtgtc gccgaggcga tcatcggcgc caccggcaag ggcgcgttca gcgtcgttgg cggcggcgat tccgcggccg cggtacgccg gctcggcctg cccgaggacg gcttctcgca catctccacc ggtggtggcg cgtcgctcga atacctcgag ggcaaggaat tgcccggcat ccaagtactg g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVKTLDDL LAEGVQGRGV LVRSDLNVPL DDDGNITDPG RVIASVPTLQ ALAEAGAKVI VTAHLGRPKG EPDPKLSLAP VAAALGEKLG RHVQLAGDVV GTDALARAEG LTDGDVLLLE NIRFDARETS KDDSERLSLA KALAALVEGP DGSPGVFVSD GFGVVHRKQA SVYDVATLLP HYAGTLVAAE VKVLQQLTSS TDRPYAVVLG GSKVSDKLAV IENLATKADS LIIGGGMCFT FLAAQGFSVG SSLLQEEMVD TCRRLLDEYA DVIHLPVDIV VADKFAADAE AETVAADRIP DGKMGLDIGP GSVERFTALL SNAKTVFWNG PMGVFEFPAF AAGTKGVAEA IIGATGKGAF SVVGGGDSAA AVRRLGLPED GFSHISTGGG ASLEYLEGKE LPGIQVLES
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGGTCAAG ACACTCGACG ATCTGCTCGC CGAGGGCGTG CAAGGTCGGG GCGTACTCGT GCGCTCCGAC CTCAACGTTC CCCTCGACGA CGACGGCAAC ATCACCGATC CGGGACGCGT CATCGCATCG GTCCCGACGC TGCAGGCCCT CGCGGAAGCG GGTGCCAAGG TGATCGTCAC CGCGCATCTC GGCCGCCCCA AGGGCGAGCC GGACCCCAAG CTGTCGCTCG CGCCGGTCGC GGCGGCACTG GGGGAGAAGC TGGGCAGGCA CGTGCAGCTC GCGGGTGACG TCGTCGGCAC CGATGCGCTC GCGCGCGCCG AGGGCCTGAC CGACGGCGAC GTGCTGCTGC TGGAGAACAT CCGGTTCGAC GCGCGCGAGA CCAGCAAGGA CGACAGCGAG CGCCTGTCGC TCGCCAAGGC CCTCGCGGCG CTCGTGGAAG GTCCCGACGG ATCACCCGGC GTCTTCGTCT CCGACGGTTT CGGTGTGGTG CACCGCAAGC AGGCGTCGGT CTACGACGTC GCGACGCTGC TGCCGCACTA CGCGGGCACG CTGGTGGCCG CCGAGGTCAA GGTGCTGCAG CAGCTGACCA GCTCGACGGA CCGTCCGTAC GCCGTCGTGC TCGGCGGCTC GAAGGTGTCC GACAAGCTCG CGGTCATCGA GAACCTCGCG ACGAAGGCCG ACAGCCTGAT CATCGGTGGC GGTATGTGCT TCACATTCCT GGCCGCACAA GGGTTTTCGG TCGGCAGCTC GCTGCTGCAG GAGGAGATGG TCGACACCTG CCGCCGCCTG CTCGACGAGT ACGCCGATGT GATCCACCTG CCGGTGGACA TCGTCGTGGC CGACAAGTTC GCCGCCGACG CGGAAGCCGA GACCGTTGCC GCCGACCGCA TCCCGGACGG CAAGATGGGC CTGGACATCG GCCCGGGCTC GGTGGAGCGG TTCACCGCGC TGCTGAGCAA CGCCAAGACC GTGTTCTGGA ACGGCCCGAT GGGTGTGTTC GAGTTCCCGG CGTTCGCGGC CGGCACCAAG GGTGTCGCCG AGGCGATCAT CGGCGCCACC GGCAAGGGCG CGTTCAGCGT CGTTGGCGGC GGCGATTCCG CGGCCGCGGT ACGCCGGCTC GGCCTGCCCG AGGACGGCTT CTCGCACATC TCCACCGGTG GTGGCGCGTC GCTCGAATAC CTCGAGGGCA AGGAATTGCC CGGCATCCAA GTACTGGAGT CGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA