MytuD.01241.a
PROBABLE THIOSULFATE SULFURTRANSFERASE SSEA (RHODANESE) (THIOSULFATE CYANIDE TRANSSULFURASE) (THIOSULFATE THIOTRANSFERASE)
| CENTER ID: | MytuD.01241.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01241.a.A1.GE25951 | Full length( MytuD.01241.a ) | 1 | 297 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01241.a.A1.PW26027 | Full length( MytuD.01241.a ) | 1 | 297 | ||
| MytuD.01241.a.A1.PS00234 | Full length( MytuD.01241.a ) | 1 | 297 |
Structures
3HZU
DEPOSITED: 6/24/2009
DETERMINATION: XRay
CLONE: MytuD.01241.a.A1.GE25951
PROTEIN: MytuD.01241.a.A1.PW26027
Publications by SSGCID
Mycobacterium thermoresistibile as a source of thermostable orthologs of Mycobacterium tuberculosis proteins.
Edwards TE, Grundner C, Liao R, Myler PJ, Phan I
Protein Sci. - 2012
volume 21, issue 7, pages 1093-6
PMID: 22544630; PMCID: PMC3403447
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|83332.12.peg.3670 |
| RefSeq: | NP_217800.1 |
| TubercuList: | Rv3283 |
| MTB Network Portal: | Rv3283 |
| UniProt: | P9WHF7 |
Orthologues for Rv3283
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| paratuberculosis | 87 | 99 | 1 | 0 | 0 |
| marinum | 85 | 98 | 0 | 0 | 0 |
| leprae | 84 | 100 | 1 | 0 | 0 |
| ulcerans | 84 | 98 | 0 | 0 | 0 |
| smegmatis | 82 | 99 | 1 | 1 | 0 |
| thermoresistibile | 79 | 99 | 1 | 2 | 1 |
| abscessus | 78 | 100 | 0 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLPADPSPT LSAYAHPERL VTADWLSAHM GAPGLAIVES DEDVLLYDVG HIPGAVKIDW HTDLNDPRVR DYINGEQFAE LMDRKGIARD DTVVIYGDKS NWWAAYALWV FTLFGHADVR LLNGGRDLWL AERRETTLDV PTKTCTGYPV VQRNDAPIRA FRDDVLAILG AQPLIDVRSP EEYTGKRTHM PDYPEEGALR AGHIPTAVHI PWGKAADESG RFRSREELER LYDFINPDDQ TVVYCRIGER SSHTWFVLTH LLGKADVRNY DGSWTEWGNA VRVPIVAGEE PGVVPVV
NT Sequence
GTGCCGCTTC CCGCAGACCC TAGCCCCACC TTGTCGGCCT ACGCCCATCC CGAACGGCTC GTGACCGCCG ACTGGTTGTC GGCACACATG GGCGCGCCGG GCCTGGCGAT CGTCGAATCC GACGAGGACG TCTTGCTCTA CGACGTCGGC CATATTCCCG GCGCCGTCAA GATCGACTGG CACACCGACC TCAACGACCC ACGGGTGCGC GACTACATCA ACGGCGAGCA GTTCGCCGAA TTGATGGACC GCAAGGGCAT CGCCCGCGAT GACACCGTGG TGATCTATGG CGACAAGAGC AATTGGTGGG CCGCCTATGC GTTGTGGGTG TTCACGCTGT TCGGTCACGC CGACGTGCGA CTCCTCAACG GCGGCCGTGA CCTCTGGCTC GCCGAGCGCC GGGAAACCAC CTTGGACGTC CCGACCAAGA CCTGCACCGG TTATCCCGTC GTGCAGCGCA ACGATGCACC CATCCGCGCA TTCAGAGACG ACGTGCTGGC CATCCTGGGC GCTCAGCCGC TGATCGACGT ACGCTCTCCC GAGGAGTACA CCGGCAAGCG CACCCATATG CCCGATTACC CCGAGGAAGG GGCGCTGCGG GCCGGTCACA TCCCCACGGC GGTGCACATT CCGTGGGGGA AGGCCGCCGA CGAAAGTGGA CGGTTTCGCA GCCGCGAGGA ATTGGAACGG CTCTATGACT TCATAAACCC GGACGACCAA ACCGTCGTCT ATTGCCGCAT CGGTGAACGC TCCAGCCATA CCTGGTTCGT GCTCACACAC CTGCTGGGCA AGGCAGATGT ACGGAACTAC GACGGCTCGT GGACCGAGTG GGGCAACGCC GTGCGAGTGC CGATCGTCGC GGGCGAAGAA CCAGGAGTGG TACCCGTCGT A
Details for MytuD.01241.a.A1.GE25951
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 7/30/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPADPS PTLSAYAHPE RLVTADWLSA HMGAPGLAIV ESDEDVLLYD VGHIPGAVKI DWHTDLNDPR VRDYINGEQF AELMDRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHAD VRLLNGGRDL WLAERRETTL DVPTKTCTGY PVVQRNDAPI RAFRDDVLAI LGAQPLIDVR SPEEYTGKRT HMPDYPEEGA LRAGHIPTAV HIPWGKAADE SGRFRSREEL ERLYDFINPD DQTVVYCRIG ERSSHTWFVL THLLGKADVR NYDGSWTEWG NAVRVPIVAG EEPGVVPVV
Validated NT Sequence
ttattatacg acgggtacca ctcctggttc ttcgcccgcg acgatcggca ctcgcacggc gttgccccac tcggtccacg agccgtcgta gttccgtaca tctgccttgc ccagcaggtg tgtgagcacg aaccaggtat ggctggagcg ttcaccgatg cggcaataga cgacggtttg gtcgtccggg tttatgaagt catagagccg ttccaattcc tcgcggctgc gaaaccgtcc actttcgtcg gcggccttcc cccacggaat gtgcaccgcc gtggggatgt gaccggcccg cagcgcccct tcctcggggt aatcgggcat atgggtgcgc ttgccggtgt actcctcggg agagcgtacg tcgatcagcg gctgagcgcc caggatggcc agcacgtcgt ctctgaatgc gcggatgggt gcatcgttgc gctgcacgac gggataaccg gtgcaggtct tggtcgggac gtccaaggtg gtttcccggc gctcggcgag ccagaggtca cggccgccgt tgaggagtcg cacgtcggcg tgaccgaaca gcgtgaacac ccacaacgca taggcggccc accaattgct cttgtcgcca tagatcacca cggtgtcatc gcgggcgatg cccttgcggt ccatcaattc ggcgaactgc tcgccgttga tgtagtcgcg cacccgtggg tcgttgaggt cggtgtgcca gtcgatcttg acggcgccgg gaatatggcc gacgtcgtag agcaagacgt cctcgtcgga ttcgacgatc gccaggcccg gcgcgcccat gtgtgccgac aaccagtcgg cggtcacgag ccgttcggga tgggcgtagg ccgacaaggt ggggctaggg tctgcgggaa gcggcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatnn ctccttctta a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPADPS PTLSAYAHPE RLVTADWLSA HMGAPGLAIV ESDEDVLLYD VGHIPGAVKI DWHTDLNDPR VRDYINGEQF AELMDRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHAD VRLLNGGRDL WLAERRETTL DVPTKTCTGY PVVQRNDAPI RAFRDDVLAI LGAQPLIDVR SPEEYTGKRT HMPDYPEEGA LRAGHIPTAV HIPWGKAADE SGRFRSREEL ERLYDFINPD DQTVVYCRIG ERSSHTWFVL THLLGKADVR NYDGSWTEWG NAVRVPIVAG EEPGVVPVV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccgctt cccgcagacc ctagccccac cttgtcggcc tacgcccatc ccgaacggct cgtgaccgcc gactggttgt cggcacacat gggcgcgccg ggcctggcga tcgtcgaatc cgacgaggac gtcttgctct acgacgtcgg ccatattccc ggcgccgtca agatcgactg gcacaccgac ctcaacgacc cacgggtgcg cgactacatc aacggcgagc agttcgccga attgatggac cgcaagggca tcgcccgcga tgacaccgtg gtgatctatg gcgacaagag caattggtgg gccgcctatg cgttgtgggt gttcacgctg ttcggtcacg ccgacgtgcg actcctcaac ggcggccgtg acctctggct cgccgagcgc cgggaaacca ccttggacgt cccgaccaag acctgcaccg gttatcccgt cgtgcagcgc aacgatgcac ccatccgcgc attcagagac gacgtgctgg ccatcctggg cgctcagccg ctgatcgacg tacgctctcc cgaggagtac accggcaagc gcacccatat gcccgattac cccgaggaag gggcgctgcg ggccggtcac atccccacgg cggtgcacat tccgtggggg aaggccgccg acgaaagtgg acggtttcgc agccgcgagg aattggaacg gctctatgac ttcataaacc cggacgacca aaccgtcgtc tattgccgca tcggtgaacg ctccagccat acctggttcg tgctcacaca cctgctgggc aaggcagatg tacggaacta cgacggctcg tggaccgagt ggggcaacgc cgtgcgagtg ccgatcgtcg cgggcgaaga accaggagtg gtacccgtcg taaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MytuD.01241.a.A1.PW26027
| PURIFICATION DATe: | 2/3/2009 |
| CONCENTRATION: | 36.56mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPADPS PTLSAYAHPE RLVTADWLSA HMGAPGLAIV ESDEDVLLYD VGHIPGAVKI DWHTDLNDPR VRDYINGEQF AELMDRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHAD VRLLNGGRDL WLAERRETTL DVPTKTCTGY PVVQRNDAPI RAFRDDVLAI LGAQPLIDVR SPEEYTGKRT HMPDYPEEGA LRAGHIPTAV HIPWGKAADE SGRFRSREEL ERLYDFINPD DQTVVYCRIG ERSSHTWFVL THLLGKADVR NYDGSWTEWG NAVRVPIVAG EEPGVVPVV
Validated NT Sequence
ttattatacg acgggtacca ctcctggttc ttcgcccgcg acgatcggca ctcgcacggc gttgccccac tcggtccacg agccgtcgta gttccgtaca tctgccttgc ccagcaggtg tgtgagcacg aaccaggtat ggctggagcg ttcaccgatg cggcaataga cgacggtttg gtcgtccggg tttatgaagt catagagccg ttccaattcc tcgcggctgc gaaaccgtcc actttcgtcg gcggccttcc cccacggaat gtgcaccgcc gtggggatgt gaccggcccg cagcgcccct tcctcggggt aatcgggcat atgggtgcgc ttgccggtgt actcctcggg agagcgtacg tcgatcagcg gctgagcgcc caggatggcc agcacgtcgt ctctgaatgc gcggatgggt gcatcgttgc gctgcacgac gggataaccg gtgcaggtct tggtcgggac gtccaaggtg gtttcccggc gctcggcgag ccagaggtca cggccgccgt tgaggagtcg cacgtcggcg tgaccgaaca gcgtgaacac ccacaacgca taggcggccc accaattgct cttgtcgcca tagatcacca cggtgtcatc gcgggcgatg cccttgcggt ccatcaattc ggcgaactgc tcgccgttga tgtagtcgcg cacccgtggg tcgttgaggt cggtgtgcca gtcgatcttg acggcgccgg gaatatggcc gacgtcgtag agcaagacgt cctcgtcgga ttcgacgatc gccaggcccg gcgcgcccat gtgtgccgac aaccagtcgg cggtcacgag ccgttcggga tgggcgtagg ccgacaaggt ggggctaggg tctgcgggaa gcggcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatnn ctccttctta a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPADPS PTLSAYAHPE RLVTADWLSA HMGAPGLAIV ESDEDVLLYD VGHIPGAVKI DWHTDLNDPR VRDYINGEQF AELMDRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHAD VRLLNGGRDL WLAERRETTL DVPTKTCTGY PVVQRNDAPI RAFRDDVLAI LGAQPLIDVR SPEEYTGKRT HMPDYPEEGA LRAGHIPTAV HIPWGKAADE SGRFRSREEL ERLYDFINPD DQTVVYCRIG ERSSHTWFVL THLLGKADVR NYDGSWTEWG NAVRVPIVAG EEPGVVPVV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCGCTT CCCGCAGACC CTAGCCCCAC CTTGTCGGCC TACGCCCATC CCGAACGGCT CGTGACCGCC GACTGGTTGT CGGCACACAT GGGCGCGCCG GGCCTGGCGA TCGTCGAATC CGACGAGGAC GTCTTGCTCT ACGACGTCGG CCATATTCCC GGCGCCGTCA AGATCGACTG GCACACCGAC CTCAACGACC CACGGGTGCG CGACTACATC AACGGCGAGC AGTTCGCCGA ATTGATGGAC CGCAAGGGCA TCGCCCGCGA TGACACCGTG GTGATCTATG GCGACAAGAG CAATTGGTGG GCCGCCTATG CGTTGTGGGT GTTCACGCTG TTCGGTCACG CCGACGTGCG ACTCCTCAAC GGCGGCCGTG ACCTCTGGCT CGCCGAGCGC CGGGAAACCA CCTTGGACGT CCCGACCAAG ACCTGCACCG GTTATCCCGT CGTGCAGCGC AACGATGCAC CCATCCGCGC ATTCAGAGAC GACGTGCTGG CCATCCTGGG CGCTCAGCCG CTGATCGACG TACGCTCTCC CGAGGAGTAC ACCGGCAAGC GCACCCATAT GCCCGATTAC CCCGAGGAAG GGGCGCTGCG GGCCGGTCAC ATCCCCACGG CGGTGCACAT TCCGTGGGGG AAGGCCGCCG ACGAAAGTGG ACGGTTTCGC AGCCGCGAGG AATTGGAACG GCTCTATGAC TTCATAAACC CGGACGACCA AACCGTCGTC TATTGCCGCA TCGGTGAACG CTCCAGCCAT ACCTGGTTCG TGCTCACACA CCTGCTGGGC AAGGCAGATG TACGGAACTA CGACGGCTCG TGGACCGAGT GGGGCAACGC CGTGCGAGTG CCGATCGTCG CGGGCGAAGA ACCAGGAGTG GTACCCGTCG TAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for MytuD.01241.a.A1.PS00234
| PURIFICATION DATe: | 7/1/2009 |
| CONCENTRATION: | 37.3mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPADPS PTLSAYAHPE RLVTADWLSA HMGAPGLAIV ESDEDVLLYD VGHIPGAVKI DWHTDLNDPR VRDYINGEQF AELMDRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHAD VRLLNGGRDL WLAERRETTL DVPTKTCTGY PVVQRNDAPI RAFRDDVLAI LGAQPLIDVR SPEEYTGKRT HMPDYPEEGA LRAGHIPTAV HIPWGKAADE SGRFRSREEL ERLYDFINPD DQTVVYCRIG ERSSHTWFVL THLLGKADVR NYDGSWTEWG NAVRVPIVAG EEPGVVPVV
Validated NT Sequence
ttattatacg acgggtacca ctcctggttc ttcgcccgcg acgatcggca ctcgcacggc gttgccccac tcggtccacg agccgtcgta gttccgtaca tctgccttgc ccagcaggtg tgtgagcacg aaccaggtat ggctggagcg ttcaccgatg cggcaataga cgacggtttg gtcgtccggg tttatgaagt catagagccg ttccaattcc tcgcggctgc gaaaccgtcc actttcgtcg gcggccttcc cccacggaat gtgcaccgcc gtggggatgt gaccggcccg cagcgcccct tcctcggggt aatcgggcat atgggtgcgc ttgccggtgt actcctcggg agagcgtacg tcgatcagcg gctgagcgcc caggatggcc agcacgtcgt ctctgaatgc gcggatgggt gcatcgttgc gctgcacgac gggataaccg gtgcaggtct tggtcgggac gtccaaggtg gtttcccggc gctcggcgag ccagaggtca cggccgccgt tgaggagtcg cacgtcggcg tgaccgaaca gcgtgaacac ccacaacgca taggcggccc accaattgct cttgtcgcca tagatcacca cggtgtcatc gcgggcgatg cccttgcggt ccatcaattc ggcgaactgc tcgccgttga tgtagtcgcg cacccgtggg tcgttgaggt cggtgtgcca gtcgatcttg acggcgccgg gaatatggcc gacgtcgtag agcaagacgt cctcgtcgga ttcgacgatc gccaggcccg gcgcgcccat gtgtgccgac aaccagtcgg cggtcacgag ccgttcggga tgggcgtagg ccgacaaggt ggggctaggg tctgcgggaa gcggcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggtatnn ctccttctta a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPADPS PTLSAYAHPE RLVTADWLSA HMGAPGLAIV ESDEDVLLYD VGHIPGAVKI DWHTDLNDPR VRDYINGEQF AELMDRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHAD VRLLNGGRDL WLAERRETTL DVPTKTCTGY PVVQRNDAPI RAFRDDVLAI LGAQPLIDVR SPEEYTGKRT HMPDYPEEGA LRAGHIPTAV HIPWGKAADE SGRFRSREEL ERLYDFINPD DQTVVYCRIG ERSSHTWFVL THLLGKADVR NYDGSWTEWG NAVRVPIVAG EEPGVVPVV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCGCTT CCCGCAGACC CTAGCCCCAC CTTGTCGGCC TACGCCCATC CCGAACGGCT CGTGACCGCC GACTGGTTGT CGGCACACAT GGGCGCGCCG GGCCTGGCGA TCGTCGAATC CGACGAGGAC GTCTTGCTCT ACGACGTCGG CCATATTCCC GGCGCCGTCA AGATCGACTG GCACACCGAC CTCAACGACC CACGGGTGCG CGACTACATC AACGGCGAGC AGTTCGCCGA ATTGATGGAC CGCAAGGGCA TCGCCCGCGA TGACACCGTG GTGATCTATG GCGACAAGAG CAATTGGTGG GCCGCCTATG CGTTGTGGGT GTTCACGCTG TTCGGTCACG CCGACGTGCG ACTCCTCAAC GGCGGCCGTG ACCTCTGGCT CGCCGAGCGC CGGGAAACCA CCTTGGACGT CCCGACCAAG ACCTGCACCG GTTATCCCGT CGTGCAGCGC AACGATGCAC CCATCCGCGC ATTCAGAGAC GACGTGCTGG CCATCCTGGG CGCTCAGCCG CTGATCGACG TACGCTCTCC CGAGGAGTAC ACCGGCAAGC GCACCCATAT GCCCGATTAC CCCGAGGAAG GGGCGCTGCG GGCCGGTCAC ATCCCCACGG CGGTGCACAT TCCGTGGGGG AAGGCCGCCG ACGAAAGTGG ACGGTTTCGC AGCCGCGAGG AATTGGAACG GCTCTATGAC TTCATAAACC CGGACGACCA AACCGTCGTC TATTGCCGCA TCGGTGAACG CTCCAGCCAT ACCTGGTTCG TGCTCACACA CCTGCTGGGC AAGGCAGATG TACGGAACTA CGACGGCTCG TGGACCGAGT GGGGCAACGC CGTGCGAGTG CCGATCGTCG CGGGCGAAGA ACCAGGAGTG GTACCCGTCG TAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA