MyleA.01241.a

Putative thiosulfate sulfurtransferase

CENTER ID: MyleA.01241.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01241.a.A1.GE29259 Full length( MyleA.01241.a ) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1327
RefSeq: YP_002503199.1
UniProt: A0A0H3MPU0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLPTDPSPS LSAYAHPERL VTGDWLYFHL GKPGLAIVES DENVLLYDVG HIPGAVKVDW HTDLNDPKVR DYITGEQFAD LMNRKGIARD DTVVIYGDKS NWWAAYALWV FTLFGHPDVR LLNGGRDLWL AERRDTSLAV PNKTSTSYPV VNRNDAPIRA FKDDVLAILG TQPLIDVRSL DEYTGKCTEM PDSPEESVLR AGHIPTARSI PWEMTVDKSG RFRSSEELER LYDFITPNDK TIVYCRIGER SSHTWFVLTH LLGKPGVRNY DGSWTEWGNT VRVPITAGES PGAVPV
NT Sequence
GTGCCGCTAC CCACAGATCC AAGCCCTTCC CTGTCGGCTT ACGCCCACCC CGAACGGCTA GTAACCGGTG ATTGGCTGTA CTTCCATCTG GGCAAACCCG GTCTGGCTAT AGTCGAATCC GACGAGAACG TACTGCTCTA CGATGTCGGA CATATTCCTG GCGCGGTGAA GGTCGACTGG CACACCGACC TCAATGACCC GAAGGTGCGT GACTACATCA CTGGCGAGCA ATTCGCCGAC TTGATGAACC GCAAGGGCAT CGCCCGCGAC GACACCGTGG TGATCTACGG CGACAAGAGC AACTGGTGGG CGGCCTACGC ACTGTGGGTC TTTACCTTGT TCGGCCATCC CGACGTGCGA CTGCTCAACG GCGGTCGTGA TCTATGGCTC GCCGAACGCC GGGATACCAG CCTGGCCGTG CCGAATAAGA CATCGACCAG CTATCCCGTG GTAAACCGGA ACGACGCACC CATCCGCGCA TTCAAAGACG ACGTGTTGGC CATCCTCGGC ACTCAGCCGC TGATCGACGT GCGATCCCTC GACGAGTACA CCGGCAAATG CACCGAAATG CCCGACTCCC CCGAAGAAAG TGTGCTGCGA GCCGGCCACA TCCCCACCGC CAGGTCGATC CCGTGGGAAA TGACAGTCGA CAAAAGCGGT CGATTCCGCA GCAGCGAAGA ATTGGAACGG CTCTATGACT TCATCACCCC AAACGATAAA ACCATCGTAT ATTGCCGCAT CGGCGAGCGA TCCAGCCACA CTTGGTTCGT ACTCACCCAT CTGCTGGGCA AACCGGGAGT GCGTAACTAT GACGGCTCGT GGACCGAGTG GGGGAACACC GTACGAGTGC CGATCACTGC AGGCGAAAGC CCCGGAGCCG TACCTGTC
Details for MyleA.01241.a.A1.GE29259
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPTDPS PSLSAYAHPE RLVTGDWLYF HLGKPGLAIV ESDENVLLYD VGHIPGAVKV DWHTDLNDPK VRDYITGEQF ADLMNRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHPD VRLLNGGRDL WLAERRDTSL AVPNKTSTSY PVVNRNDAPI RAFKDDVLAI LGTQPLIDVR SLDEYTGKCT EMPDSPEESV LRAGHIPTAR SIPWEMTVDK SGRFRSSEEL ERLYDFITPN DKTIVYCRIG ERSSHTWFVL THLLGKPGVR NYDGSWTEWG NTVRVPITAG ESPGAVPV
Validated NT Sequence
ttattagaca ggtacggctc cggggctttc gcctgcagtg atcggcactc gtacggtgtt cccccactcg gtccacgagc cgtcatagtt acgcactccc ggtttgccca gcagatgggt gagtacgaac caagtgtggc tggatcgctc gccgatgcgg caatatacga tggttttatc gtttggggtg atgaagtcat agagccgttc caattcttcg ctgctgcgga atcgaccgct tttgtcgact gtcatttccc acgggatcga cctggcggtg gggatgtggc cggctcgcag cacactttct tcgggggagt cgggcatttc ggtgcatttg ccggtgtact cgtcgaggga tcgcacgtcg atcagcggct gagtgccgag gatggccaac acgtcgtctt tgaatgcgcg gatgggtgcg tcgttccggt ttaccacggg atagctggtc gatgtcttat tcggcacggc caggctggta tcccggcgtt cggcgagcca tagatcacga ccgccgttga gcagtcgcac gtcgggatgg ccgaacaagg taaagaccca cagtgcgtag gccgcccacc agttgctctt gtcgccgtag atcaccacgg tgtcgtcgcg ggcgatgccc ttgcggttca tcaagtcggc gaattgctcg ccagtgatgt agtcacgcac cttcgggtca ttgaggtcgg tgtgccagtc gaccttcacc gcgccaggaa tatgtccgac atcgtagagc agtacgttct cgtcggattc gactatagcc agaccgggtt tgcccagatg gaagtacagc caatcaccgg ttactagccg ttcggggtgg gcgtaagccg acagggaagg gcttggatct gtgggtagcg gcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPLPTDPS PSLSAYAHPE RLVTGDWLYF HLGKPGLAIV ESDENVLLYD VGHIPGAVKV DWHTDLNDPK VRDYITGEQF ADLMNRKGIA RDDTVVIYGD KSNWWAAYAL WVFTLFGHPD VRLLNGGRDL WLAERRDTSL AVPNKTSTSY PVVNRNDAPI RAFKDDVLAI LGTQPLIDVR SLDEYTGKCT EMPDSPEESV LRAGHIPTAR SIPWEMTVDK SGRFRSSEEL ERLYDFITPN DKTIVYCRIG ERSSHTWFVL THLLGKPGVR NYDGSWTEWG NTVRVPITAG ESPGAVPV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccgcta cccacagatc caagcccttc cctgtcggct tacgcccacc ccgaacggct agtaaccggt gattggctgt acttccatct gggcaaaccc ggtctggcta tagtcgaatc cgacgagaac gtactgctct acgatgtcgg acatattcct ggcgcggtga aggtcgactg gcacaccgac ctcaatgacc cgaaggtgcg tgactacatc actggcgagc aattcgccga cttgatgaac cgcaagggca tcgcccgcga cgacaccgtg gtgatctacg gcgacaagag caactggtgg gcggcctacg cactgtgggt ctttaccttg ttcggccatc ccgacgtgcg actgctcaac ggcggtcgtg atctatggct cgccgaacgc cgggatacca gcctggccgt gccgaataag acatcgacca gctatcccgt ggtaaaccgg aacgacgcac ccatccgcgc attcaaagac gacgtgttgg ccatcctcgg cactcagccg ctgatcgacg tgcgatccct cgacgagtac accggcaaat gcaccgaaat gcccgactcc cccgaagaaa gtgtgctgcg agccggccac atccccaccg ccaggtcgat cccgtgggaa atgacagtcg acaaaagcgg tcgattccgc agcagcgaag aattggaacg gctctatgac ttcatcaccc caaacgataa aaccatcgta tattgccgca tcggcgagcg atccagccac acttggttcg tactcaccca tctgctgggc aaaccgggag tgcgtaacta tgacggctcg tggaccgagt gggggaacac cgtacgagtg ccgatcactg caggcgaaag ccccggagcc gtacctgtca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a