MysmA.01241.a

Putative thiosulfate sulfurtransferase

CENTER ID: MysmA.01241.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01241.a.A1.GE28891 Full length( MysmA.01241.a ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01241.a.A1.PW28796 Full length( MysmA.01241.a ) 1 295
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127129
PATRIC ID: fig|246196.19.peg.1789
RefSeq: YP_886181.1
UniProt: A0QTE3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLPADPSPT LAEYAHPERL VTADWLASNL GRPGLAIVES DEDVLLYDTG HIPGAVKIDW HTDLNDPHVR DYINGEQFAA LMDRKGISRD DTVVIYGDKS NWWAAYALWV FTLFGHPDVR LLDGGRDLWV SHGRDTTLDV PTRQSSGYPV VERNDAPIRA FKEDVLEILG KQPLIDVRSP QEYTGERTHM PDYPEEGALR GGHIPTAKSI PWGKAAKDNG QFRSRAELEE LYGFLTPDDE TVVYCRIGER SSHTWFVLTH LLGLPGVRNY DGSWTEWGNA VRVPVAVGPD PGSAP
NT Sequence
GTGTCGCTGC CTGCAGATCC CAGCCCCACT CTCGCCGAAT ACGCCCACCC GGAACGCCTG GTGACCGCCG ACTGGCTCGC CAGTAACCTC GGTCGGCCCG GCCTGGCCAT CGTCGAGTCC GACGAAGACG TCCTCCTCTA CGACACCGGA CACATCCCCG GGGCGGTGAA GATCGACTGG CACACCGACC TCAACGACCC CCACGTGCGG GACTACATCA ACGGTGAGCA GTTCGCCGCG TTGATGGACC GCAAGGGCAT CAGCCGCGAC GACACCGTCG TCATCTACGG CGACAAGAGC AACTGGTGGG CCGCATACGC CCTGTGGGTC TTCACGCTGT TCGGTCACCC GGATGTGCGC CTGCTCGACG GCGGGCGCGA CCTGTGGGTC TCACACGGTC GTGACACCAC CCTGGACGTG CCGACGCGCC AGTCGAGCGG GTACCCGGTC GTCGAGCGCA ACGACGCCCC GATCCGCGCC TTCAAAGAGG ACGTGCTGGA GATTCTCGGC AAGCAGCCGC TGATCGACGT CCGCTCGCCG CAGGAGTACA CCGGTGAACG GACCCACATG CCGGATTACC CGGAGGAAGG TGCGTTGCGG GGTGGCCACA TCCCCACCGC CAAATCCATC CCCTGGGGCA AGGCCGCCAA GGACAACGGC CAGTTCCGCA GCCGCGCCGA GCTCGAAGAG CTGTACGGCT TCCTCACGCC CGATGACGAG ACCGTGGTGT ACTGCCGCAT CGGTGAGCGT TCGAGCCACA CCTGGTTCGT GTTGACCCAC CTGCTGGGCC TGCCGGGCGT CCGCAACTAC GACGGTTCGT GGACCGAGTG GGGCAACGCG GTGCGGGTGC CGGTCGCGGT CGGTCCGGAC CCGGGTTCGG CACCG
Details for MysmA.01241.a.A1.GE28891
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVTADWLAS NLGRPGLAIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPH VRDYINGEQF AALMDRKGIS RDDTVVIYGD KSNWWAAYAL WVFTLFGHPD VRLLDGGRDL WVSHGRDTTL DVPTRQSSGY PVVERNDAPI RAFKEDVLEI LGKQPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAK SIPWGKAAKD NGQFRSRAEL EELYGFLTPD DETVVYCRIG ERSSHTWFVL THLLGLPGVR NYDGSWTEWG NAVRVPVAVG PDPGSAP
Validated NT Sequence
ttattacggt gccgaacccg ggtccggacc gaccgcgacc ggcacccgca ccgcgttgcc ccactcggtc cacgaaccgt cgtagttgcg gacgcccggc aggcccagca ggtgggtcaa cacgaaccag gtgtggctcg aacgctcacc gatgcggcag tacaccacgg tctcgtcatc gggcgtgagg aagccgtaca gctcttcgag ctcggcgcgg ctgcggaact ggccgttgtc cttggcggcc ttgccccagg ggatggattt ggcggtgggg atgtggccac cccgcaacgc accttcctcc gggtaatccg gcatgtgggt ccgttcaccg gtgtactcct gcggcgagcg gacgtcgatc agcggctgct tgccgagaat ctccagcacg tcctctttga aggcgcggat cggggcgtcg ttgcgctcga cgaccgggta cccgctcgac tggcgcgtcg gcacgtccag ggtggtgtca cgaccgtgtg agacccacag gtcgcgcccg ccgtcgagca ggcgcacatc cgggtgaccg aacagcgtga agacccacag ggcgtatgcg gcccaccagt tgctcttgtc gccgtagatg acgacggtgt cgtcgcggct gatgcccttg cggtccatca acgcggcgaa ctgctcaccg ttgatgtagt cccgcacgtg ggggtcgttg aggtcggtgt gccagtcgat cttcaccgcc ccggggatgt gtccggtgtc gtagaggagg acgtcttcgt cggactcgac gatggccagg ccgggccgac cgaggttact ggcgagccag tcggcggtca ccaggcgttc cgggtgggcg tattcggcga gagtggggct gggatctgca ggcagcgaca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVTADWLAS NLGRPGLAIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPH VRDYINGEQF AALMDRKGIS RDDTVVIYGD KSNWWAAYAL WVFTLFGHPD VRLLDGGRDL WVSHGRDTTL DVPTRQSSGY PVVERNDAPI RAFKEDVLEI LGKQPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAK SIPWGKAAKD NGQFRSRAEL EELYGFLTPD DETVVYCRIG ERSSHTWFVL THLLGLPGVR NYDGSWTEWG NAVRVPVAVG PDPGSAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcgctg cctgcagatc ccagccccac tctcgccgaa tacgcccacc cggaacgcct ggtgaccgcc gactggctcg ccagtaacct cggtcggccc ggcctggcca tcgtcgagtc cgacgaagac gtcctcctct acgacaccgg acacatcccc ggggcggtga agatcgactg gcacaccgac ctcaacgacc cccacgtgcg ggactacatc aacggtgagc agttcgccgc gttgatggac cgcaagggca tcagccgcga cgacaccgtc gtcatctacg gcgacaagag caactggtgg gccgcatacg ccctgtgggt cttcacgctg ttcggtcacc cggatgtgcg cctgctcgac ggcgggcgcg acctgtgggt ctcacacggt cgtgacacca ccctggacgt gccgacgcgc cagtcgagcg ggtacccggt cgtcgagcgc aacgacgccc cgatccgcgc cttcaaagag gacgtgctgg agattctcgg caagcagccg ctgatcgacg tccgctcgcc gcaggagtac accggtgaac ggacccacat gccggattac ccggaggaag gtgcgttgcg gggtggccac atccccaccg ccaaatccat cccctggggc aaggccgcca aggacaacgg ccagttccgc agccgcgccg agctcgaaga gctgtacggc ttcctcacgc ccgatgacga gaccgtggtg tactgccgca tcggtgagcg ttcgagccac acctggttcg tgttgaccca cctgctgggc ctgccgggcg tccgcaacta cgacggttcg tggaccgagt ggggcaacgc ggtgcgggtg ccggtcgcgg tcggtccgga cccgggttcg gcaccgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MysmA.01241.a.A1.PW28796
PURIFICATION DATe: 6/1/2010
CONCENTRATION: 25.81mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVTADWLAS NLGRPGLAIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPH VRDYINGEQF AALMDRKGIS RDDTVVIYGD KSNWWAAYAL WVFTLFGHPD VRLLDGGRDL WVSHGRDTTL DVPTRQSSGY PVVERNDAPI RAFKEDVLEI LGKQPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAK SIPWGKAAKD NGQFRSRAEL EELYGFLTPD DETVVYCRIG ERSSHTWFVL THLLGLPGVR NYDGSWTEWG NAVRVPVAVG PDPGSAP
Validated NT Sequence
ttattacggt gccgaacccg ggtccggacc gaccgcgacc ggcacccgca ccgcgttgcc ccactcggtc cacgaaccgt cgtagttgcg gacgcccggc aggcccagca ggtgggtcaa cacgaaccag gtgtggctcg aacgctcacc gatgcggcag tacaccacgg tctcgtcatc gggcgtgagg aagccgtaca gctcttcgag ctcggcgcgg ctgcggaact ggccgttgtc cttggcggcc ttgccccagg ggatggattt ggcggtgggg atgtggccac cccgcaacgc accttcctcc gggtaatccg gcatgtgggt ccgttcaccg gtgtactcct gcggcgagcg gacgtcgatc agcggctgct tgccgagaat ctccagcacg tcctctttga aggcgcggat cggggcgtcg ttgcgctcga cgaccgggta cccgctcgac tggcgcgtcg gcacgtccag ggtggtgtca cgaccgtgtg agacccacag gtcgcgcccg ccgtcgagca ggcgcacatc cgggtgaccg aacagcgtga agacccacag ggcgtatgcg gcccaccagt tgctcttgtc gccgtagatg acgacggtgt cgtcgcggct gatgcccttg cggtccatca acgcggcgaa ctgctcaccg ttgatgtagt cccgcacgtg ggggtcgttg aggtcggtgt gccagtcgat cttcaccgcc ccggggatgt gtccggtgtc gtagaggagg acgtcttcgt cggactcgac gatggccagg ccgggccgac cgaggttact ggcgagccag tcggcggtca ccaggcgttc cgggtgggcg tattcggcga gagtggggct gggatctgca ggcagcgaca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVTADWLAS NLGRPGLAIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPH VRDYINGEQF AALMDRKGIS RDDTVVIYGD KSNWWAAYAL WVFTLFGHPD VRLLDGGRDL WVSHGRDTTL DVPTRQSSGY PVVERNDAPI RAFKEDVLEI LGKQPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAK SIPWGKAAKD NGQFRSRAEL EELYGFLTPD DETVVYCRIG ERSSHTWFVL THLLGLPGVR NYDGSWTEWG NAVRVPVAVG PDPGSAP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCGCTG CCTGCAGATC CCAGCCCCAC TCTCGCCGAA TACGCCCACC CGGAACGCCT GGTGACCGCC GACTGGCTCG CCAGTAACCT CGGTCGGCCC GGCCTGGCCA TCGTCGAGTC CGACGAAGAC GTCCTCCTCT ACGACACCGG ACACATCCCC GGGGCGGTGA AGATCGACTG GCACACCGAC CTCAACGACC CCCACGTGCG GGACTACATC AACGGTGAGC AGTTCGCCGC GTTGATGGAC CGCAAGGGCA TCAGCCGCGA CGACACCGTC GTCATCTACG GCGACAAGAG CAACTGGTGG GCCGCATACG CCCTGTGGGT CTTCACGCTG TTCGGTCACC CGGATGTGCG CCTGCTCGAC GGCGGGCGCG ACCTGTGGGT CTCACACGGT CGTGACACCA CCCTGGACGT GCCGACGCGC CAGTCGAGCG GGTACCCGGT CGTCGAGCGC AACGACGCCC CGATCCGCGC CTTCAAAGAG GACGTGCTGG AGATTCTCGG CAAGCAGCCG CTGATCGACG TCCGCTCGCC GCAGGAGTAC ACCGGTGAAC GGACCCACAT GCCGGATTAC CCGGAGGAAG GTGCGTTGCG GGGTGGCCAC ATCCCCACCG CCAAATCCAT CCCCTGGGGC AAGGCCGCCA AGGACAACGG CCAGTTCCGC AGCCGCGCCG AGCTCGAAGA GCTGTACGGC TTCCTCACGC CCGATGACGA GACCGTGGTG TACTGCCGCA TCGGTGAGCG TTCGAGCCAC ACCTGGTTCG TGTTGACCCA CCTGCTGGGC CTGCCGGGCG TCCGCAACTA CGACGGTTCG TGGACCGAGT GGGGCAACGC GGTGCGGGTG CCGGTCGCGG TCGGTCCGGA CCCGGGTTCG GCACCGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA