MythA.01241.a
Putative thiosulfate sulfurtransferase (A0QTE3 homolog)
| CENTER ID: | MythA.01241.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01241.a.A1.GE28773 | Full length( MythA.01241.a ) | 1 | 298 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01241.a.A1.PS00652 | Full length( MythA.01241.a ) | 1 | 298 | ||
| MythA.01241.a.A1.PS00655 | Full length( MythA.01241.a ) | 1 | 298 |
Structures
3P3A
DEPOSITED: 10/4/2010
DETERMINATION: XRay
CLONE: MythA.01241.a.A1.GE28773
PROTEIN: MythA.01241.a.A1.PS00652
Publications by SSGCID
Mycobacterium thermoresistibile as a source of thermostable orthologs of Mycobacterium tuberculosis proteins.
Edwards TE, Grundner C, Liao R, Myler PJ, Phan I
Protein Sci. - 2012
volume 21, issue 7, pages 1093-6
PMID: 22544630; PMCID: PMC3403447
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.4501 |
| RefSeq: | WP_003928012.1 |
| UniProt: | G7CNF6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLPADPSPT LAEYAHPERL VPADWLASNL GRPGLVIVES DEDVLLYDTG HIPGAVKIDW HTDLNDPAVR DYINGEQFAA LMDRKGVTRD DTVVIYGDKS NWWAAYAMWV FHLFGHPDVR LLDGGRDLWV STGRDTTLEV PTRQTSGYPV VERNDAPIRA FKDDVLRVLG KEPLIDVRSP QEYTGERTHM PDYPEEGALR GGHIPTAVSV PWSKAAYDDG RFRARAELEE IYGFVKPDDK PIVYCRIGER SSHTWFVLTY LLGHPNVRNY DGSWTEWGNA VRVPIVVGPE PGEAPGTS
NT Sequence
GTGTCGCTGC CTGCAGATCC CAGCCCCACT CTCGCCGAAT ACGCCCACCC GGAACGCCTG GTCCCCGCCG ACTGGCTGGC CAGCAACCTC GGTCGCCCGG GCCTGGTCAT CGTCGAGTCC GATGAGGATG TGCTGCTCTA CGACACCGGC CACATCCCCG GTGCCGTCAA GATCGACTGG CACACCGACC TCAACGACCC GGCGGTGCGC GACTACATCA ACGGCGAGCA GTTCGCCGCG CTGATGGACC GGAAAGGCGT CACCCGGGAC GACACCGTGG TGATCTACGG CGACAAGAGC AACTGGTGGG CCGCCTACGC GATGTGGGTG TTCCACCTGT TCGGCCACCC CGATGTGCGT CTGCTCGACG GTGGACGCGA TCTGTGGGTG TCCACCGGAC GTGACACCAC CCTCGAGGTG CCCACCCGGC AGACCTCCGG CTACCCGGTC GTGGAGCGCA ACGACGCGCC CATCCGCGCC TTCAAGGACG ACGTTCTGCG GGTGCTCGGC AAGGAACCGC TGATCGACGT GCGCTCACCG CAGGAGTACA CCGGCGAACG CACCCACATG CCCGACTATC CGGAGGAGGG CGCGCTGCGC GGCGGGCACA TCCCGACCGC CGTGTCGGTC CCGTGGTCGA AAGCGGCCTA CGACGACGGG CGGTTCCGCG CCCGCGCCGA ACTCGAGGAG ATCTACGGCT TCGTCAAGCC GGACGACAAG CCCATCGTCT ACTGCCGAAT CGGCGAGCGC TCCAGCCACA CCTGGTTCGT GTTGACCTAC CTGCTCGGCC ATCCGAACGT CCGTAACTAC GACGGGTCGT GGACCGAATG GGGCAACGCG GTGCGGGTGC CGATCGTCGT CGGTCCGGAA CCCGGCGAGG CTCCCGGGAC ATCG
Details for MythA.01241.a.A1.GE28773
| HARVESTED ON: | 3/1/2010 |
| SEQUENCED ON: | 5/19/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PELKQYAHPE RLVTADWLAS NLGRPGLVIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPA VRDYINGEQF AALMDRKGVT RDDTVVIYGD KSNWWAAYAM WVFHLFGHPD VRLLDGGRDL WVSTGRDTTL EVPTRQTSGY PVVERNDAPI RAFKDDVLRV LGKEPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAV SVPWSKAAYD DGRFRARAEL EEIYGFVKPD DKPIVYCRIG ERSSHTWFVL TYLLGHPNVR NYDGSWTEWG NAVRVPIVVG PEPGEAPGTS
Validated NT Sequence
ttattacgat gtcccgggag cctcgccggg ttccggaccg acgacgatcg gcacccgcac cgcgttgccc cattcggtcc acgacccgtc gtagttacgg acgttcggat ggccgagcag gtaggtcaac acgaaccagg tgtggctgga gcgctcgccg attcggcagt agacgatggg cttgtcgtcc ggcttgacga agccgtagat ctcctcgagt tcggcgcggg cgcggaaccg cccgtcgtcg taggccgctt tcgaccacgg gaccgacacg gcggtcggga tgtgcccgcc gcgcagcgcg ccctcctccg gatagtcggg catgtgggtg cgttcgccgg tgtactcctg cggtgagcgc acgtcgatca gcggttcctt gccgagcacc cgcagaacgt cgtccttgaa ggcgcggatg ggcgcgtcgt tgcgctccac gaccgggtag ccggaggtct gccgggtggg cacctcgagg gtggtgtcac gtccggtgga cacccacaga tcgcgtccac cgtcgagcag acgcacatcg gggtggccga acaggtggaa cacccacatc gcgtaggcgg cccaccagtt gctcttgtcg ccgtagatca ccacggtgtc gtcccgggtg acgcctttcc ggtccatcag cgcggcgaac tgctcgccgt tgatgtagtc gcgcaccgcc gggtcgttga ggtcggtgtg ccagtcgatc ttgacggcac cggggatgtg gccggtgtcg tagagcagca catcctcatc ggactcgacg atgaccaggc ccgggcgacc gaggttgctg gccagccagt cggcggtgac cagacgctcg gggtgggcgt attgcttcag ttcagggctg ggatctgcag gcagcgacac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tatctccttc ttaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVPADWLAS NLGRPGLVIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPA VRDYINGEQF AALMDRKGVT RDDTVVIYGD KSNWWAAYAM WVFHLFGHPD VRLLDGGRDL WVSTGRDTTL EVPTRQTSGY PVVERNDAPI RAFKDDVLRV LGKEPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAV SVPWSKAAYD DGRFRARAEL EEIYGFVKPD DKPIVYCRIG ERSSHTWFVL TYLLGHPNVR NYDGSWTEWG NAVRVPIVVG PEPGEAPGTS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcgctg cctgcagatc ccagccccac tctcgccgaa tacgcccacc cggaacgcct ggtccccgcc gactggctgg ccagcaacct cggtcgcccg ggcctggtca tcgtcgagtc cgatgaggat gtgctgctct acgacaccgg ccacatcccc ggtgccgtca agatcgactg gcacaccgac ctcaacgacc cggcggtgcg cgactacatc aacggcgagc agttcgccgc gctgatggac cggaaaggcg tcacccggga cgacaccgtg gtgatctacg gcgacaagag caactggtgg gccgcctacg cgatgtgggt gttccacctg ttcggccacc ccgatgtgcg tctgctcgac ggtggacgcg atctgtgggt gtccaccgga cgtgacacca ccctcgaggt gcccacccgg cagacctccg gctacccggt cgtggagcgc aacgacgcgc ccatccgcgc cttcaaggac gacgttctgc gggtgctcgg caaggaaccg ctgatcgacg tgcgctcacc gcaggagtac accggcgaac gcacccacat gcccgactat ccggaggagg gcgcgctgcg cggcgggcac atcccgaccg ccgtgtcggt cccgtggtcg aaagcggcct acgacgacgg gcggttccgc gcccgcgccg aactcgagga gatctacggc ttcgtcaagc cggacgacaa gcccatcgtc tactgccgaa tcggcgagcg ctccagccac acctggttcg tgttgaccta cctgctcggc catccgaacg tccgtaacta cgacgggtcg tggaccgaat ggggcaacgc ggtgcgggtg ccgatcgtcg tcggtccgga acccggcgag gctcccggga catcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MythA.01241.a.A1.PS00652
| PURIFICATION DATe: | 6/29/2010 |
| CONCENTRATION: | 48.15mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PELKQYAHPE RLVTADWLAS NLGRPGLVIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPA VRDYINGEQF AALMDRKGVT RDDTVVIYGD KSNWWAAYAM WVFHLFGHPD VRLLDGGRDL WVSTGRDTTL EVPTRQTSGY PVVERNDAPI RAFKDDVLRV LGKEPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAV SVPWSKAAYD DGRFRARAEL EEIYGFVKPD DKPIVYCRIG ERSSHTWFVL TYLLGHPNVR NYDGSWTEWG NAVRVPIVVG PEPGEAPGTS
Validated NT Sequence
ttattacgat gtcccgggag cctcgccggg ttccggaccg acgacgatcg gcacccgcac cgcgttgccc cattcggtcc acgacccgtc gtagttacgg acgttcggat ggccgagcag gtaggtcaac acgaaccagg tgtggctgga gcgctcgccg attcggcagt agacgatggg cttgtcgtcc ggcttgacga agccgtagat ctcctcgagt tcggcgcggg cgcggaaccg cccgtcgtcg taggccgctt tcgaccacgg gaccgacacg gcggtcggga tgtgcccgcc gcgcagcgcg ccctcctccg gatagtcggg catgtgggtg cgttcgccgg tgtactcctg cggtgagcgc acgtcgatca gcggttcctt gccgagcacc cgcagaacgt cgtccttgaa ggcgcggatg ggcgcgtcgt tgcgctccac gaccgggtag ccggaggtct gccgggtggg cacctcgagg gtggtgtcac gtccggtgga cacccacaga tcgcgtccac cgtcgagcag acgcacatcg gggtggccga acaggtggaa cacccacatc gcgtaggcgg cccaccagtt gctcttgtcg ccgtagatca ccacggtgtc gtcccgggtg acgcctttcc ggtccatcag cgcggcgaac tgctcgccgt tgatgtagtc gcgcaccgcc gggtcgttga ggtcggtgtg ccagtcgatc ttgacggcac cggggatgtg gccggtgtcg tagagcagca catcctcatc ggactcgacg atgaccaggc ccgggcgacc gaggttgctg gccagccagt cggcggtgac cagacgctcg gggtgggcgt attgcttcag ttcagggctg ggatctgcag gcagcgacac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tatctccttc ttaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVPADWLAS NLGRPGLVIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPA VRDYINGEQF AALMDRKGVT RDDTVVIYGD KSNWWAAYAM WVFHLFGHPD VRLLDGGRDL WVSTGRDTTL EVPTRQTSGY PVVERNDAPI RAFKDDVLRV LGKEPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAV SVPWSKAAYD DGRFRARAEL EEIYGFVKPD DKPIVYCRIG ERSSHTWFVL TYLLGHPNVR NYDGSWTEWG NAVRVPIVVG PEPGEAPGTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCGCTG CCTGCAGATC CCAGCCCCAC TCTCGCCGAA TACGCCCACC CGGAACGCCT GGTCCCCGCC GACTGGCTGG CCAGCAACCT CGGTCGCCCG GGCCTGGTCA TCGTCGAGTC CGATGAGGAT GTGCTGCTCT ACGACACCGG CCACATCCCC GGTGCCGTCA AGATCGACTG GCACACCGAC CTCAACGACC CGGCGGTGCG CGACTACATC AACGGCGAGC AGTTCGCCGC GCTGATGGAC CGGAAAGGCG TCACCCGGGA CGACACCGTG GTGATCTACG GCGACAAGAG CAACTGGTGG GCCGCCTACG CGATGTGGGT GTTCCACCTG TTCGGCCACC CCGATGTGCG TCTGCTCGAC GGTGGACGCG ATCTGTGGGT GTCCACCGGA CGTGACACCA CCCTCGAGGT GCCCACCCGG CAGACCTCCG GCTACCCGGT CGTGGAGCGC AACGACGCGC CCATCCGCGC CTTCAAGGAC GACGTTCTGC GGGTGCTCGG CAAGGAACCG CTGATCGACG TGCGCTCACC GCAGGAGTAC ACCGGCGAAC GCACCCACAT GCCCGACTAT CCGGAGGAGG GCGCGCTGCG CGGCGGGCAC ATCCCGACCG CCGTGTCGGT CCCGTGGTCG AAAGCGGCCT ACGACGACGG GCGGTTCCGC GCCCGCGCCG AACTCGAGGA GATCTACGGC TTCGTCAAGC CGGACGACAA GCCCATCGTC TACTGCCGAA TCGGCGAGCG CTCCAGCCAC ACCTGGTTCG TGTTGACCTA CCTGCTCGGC CATCCGAACG TCCGTAACTA CGACGGGTCG TGGACCGAAT GGGGCAACGC GGTGCGGGTG CCGATCGTCG TCGGTCCGGA ACCCGGCGAG GCTCCCGGGA CATCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for MythA.01241.a.A1.PS00655
| PURIFICATION DATe: | 6/29/2010 |
| CONCENTRATION: | 33.79mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PELKQYAHPE RLVTADWLAS NLGRPGLVIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPA VRDYINGEQF AALMDRKGVT RDDTVVIYGD KSNWWAAYAM WVFHLFGHPD VRLLDGGRDL WVSTGRDTTL EVPTRQTSGY PVVERNDAPI RAFKDDVLRV LGKEPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAV SVPWSKAAYD DGRFRARAEL EEIYGFVKPD DKPIVYCRIG ERSSHTWFVL TYLLGHPNVR NYDGSWTEWG NAVRVPIVVG PEPGEAPGTS
Validated NT Sequence
ttattacgat gtcccgggag cctcgccggg ttccggaccg acgacgatcg gcacccgcac cgcgttgccc cattcggtcc acgacccgtc gtagttacgg acgttcggat ggccgagcag gtaggtcaac acgaaccagg tgtggctgga gcgctcgccg attcggcagt agacgatggg cttgtcgtcc ggcttgacga agccgtagat ctcctcgagt tcggcgcggg cgcggaaccg cccgtcgtcg taggccgctt tcgaccacgg gaccgacacg gcggtcggga tgtgcccgcc gcgcagcgcg ccctcctccg gatagtcggg catgtgggtg cgttcgccgg tgtactcctg cggtgagcgc acgtcgatca gcggttcctt gccgagcacc cgcagaacgt cgtccttgaa ggcgcggatg ggcgcgtcgt tgcgctccac gaccgggtag ccggaggtct gccgggtggg cacctcgagg gtggtgtcac gtccggtgga cacccacaga tcgcgtccac cgtcgagcag acgcacatcg gggtggccga acaggtggaa cacccacatc gcgtaggcgg cccaccagtt gctcttgtcg ccgtagatca ccacggtgtc gtcccgggtg acgcctttcc ggtccatcag cgcggcgaac tgctcgccgt tgatgtagtc gcgcaccgcc gggtcgttga ggtcggtgtg ccagtcgatc ttgacggcac cggggatgtg gccggtgtcg tagagcagca catcctcatc ggactcgacg atgaccaggc ccgggcgacc gaggttgctg gccagccagt cggcggtgac cagacgctcg gggtgggcgt attgcttcag ttcagggctg ggatctgcag gcagcgacac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tatctccttc ttaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLPADPS PTLAEYAHPE RLVPADWLAS NLGRPGLVIV ESDEDVLLYD TGHIPGAVKI DWHTDLNDPA VRDYINGEQF AALMDRKGVT RDDTVVIYGD KSNWWAAYAM WVFHLFGHPD VRLLDGGRDL WVSTGRDTTL EVPTRQTSGY PVVERNDAPI RAFKDDVLRV LGKEPLIDVR SPQEYTGERT HMPDYPEEGA LRGGHIPTAV SVPWSKAAYD DGRFRARAEL EEIYGFVKPD DKPIVYCRIG ERSSHTWFVL TYLLGHPNVR NYDGSWTEWG NAVRVPIVVG PEPGEAPGTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCGCTG CCTGCAGATC CCAGCCCCAC TCTCGCCGAA TACGCCCACC CGGAACGCCT GGTCCCCGCC GACTGGCTGG CCAGCAACCT CGGTCGCCCG GGCCTGGTCA TCGTCGAGTC CGATGAGGAT GTGCTGCTCT ACGACACCGG CCACATCCCC GGTGCCGTCA AGATCGACTG GCACACCGAC CTCAACGACC CGGCGGTGCG CGACTACATC AACGGCGAGC AGTTCGCCGC GCTGATGGAC CGGAAAGGCG TCACCCGGGA CGACACCGTG GTGATCTACG GCGACAAGAG CAACTGGTGG GCCGCCTACG CGATGTGGGT GTTCCACCTG TTCGGCCACC CCGATGTGCG TCTGCTCGAC GGTGGACGCG ATCTGTGGGT GTCCACCGGA CGTGACACCA CCCTCGAGGT GCCCACCCGG CAGACCTCCG GCTACCCGGT CGTGGAGCGC AACGACGCGC CCATCCGCGC CTTCAAGGAC GACGTTCTGC GGGTGCTCGG CAAGGAACCG CTGATCGACG TGCGCTCACC GCAGGAGTAC ACCGGCGAAC GCACCCACAT GCCCGACTAT CCGGAGGAGG GCGCGCTGCG CGGCGGGCAC ATCCCGACCG CCGTGTCGGT CCCGTGGTCG AAAGCGGCCT ACGACGACGG GCGGTTCCGC GCCCGCGCCG AACTCGAGGA GATCTACGGC TTCGTCAAGC CGGACGACAA GCCCATCGTC TACTGCCGAA TCGGCGAGCG CTCCAGCCAC ACCTGGTTCG TGTTGACCTA CCTGCTCGGC CATCCGAACG TCCGTAACTA CGACGGGTCG TGGACCGAAT GGGGCAACGC GGTGCGGGTG CCGATCGTCG TCGGTCCGGA ACCCGGCGAG GCTCCCGGGA CATCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA