MytuD.01117.a

PROBABLE ACYL-CoA DEHYDROGENASE FADE29

CENTER ID: MytuD.01117.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01117.a.A1.GE25933 Full length( MytuD.01117.a ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_239169
PATRIC ID: fig|83332.12.peg.3957
RefSeq: NP_218060.1
TubercuList: Rv3543c
MTB Network Portal: Rv3543c
UniProt: P71858

Orthologues for Rv3543c

Species % Identity % Coverage Clones Proteins Structures
marinum 90 100 0 0 0
ulcerans 90 100 1 0 0
paratuberculosis 88 100 1 1 0
thermoresistibile 82 77 1 0 0
smegmatis 78 100 0 0 0
abscessus 75 100 0 0 0
avium 33 73 1 1 0
paratuberculosis 33 73 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIDLTPEQR QLQAEIRQYF SNLISPDERT EMEKDRHGPA YRAVIRRMGR DGRLGVGWPK EFGGLGFGPI EQQIFVNEAH RADVPLPAVT LQTVGPTLQA HGSELQKKKF LPAILAGEAH FAIGYTEPEA GTDLASLRTT AVRDGDHYIV NGQKVFTTGA HDADYIWLAC RTDPNAAKHK GISILIVDTK DPGYSWTPII LADGAHHTNA TYYNDVRVPV DMLVGKENDG WRLITTQLNN ERVMLGPAGR FASIYDRVHA WASVPGGNGV TPIDHDDVKR ALGEIRAIWR INELLNWQVA SAGEDINMAD AAATKVFGTE RVQRAGRLAE EIVGKYGNPA EPDTAELLRW LDAQTKRNLV ITFGGGVNEV MREMIAASGL KVPRVPR
NT Sequence
ATGTTCATTG ACCTGACGCC GGAGCAGCGC CAGCTGCAAG CCGAGATACG GCAATACTTT TCGAATCTCA TCTCGCCCGA CGAGCGCACG GAGATGGAGA AAGACCGGCA CGGCCCGGCC TACCGTGCGG TGATACGGCG CATGGGCCGG GACGGCAGGC TCGGTGTTGG GTGGCCGAAA GAGTTCGGCG GTCTGGGTTT TGGTCCGATC GAGCAGCAGA TCTTCGTCAA CGAGGCCCAT CGCGCCGACG TGCCGCTGCC CGCGGTGACG CTGCAGACCG TCGGCCCCAC GCTGCAGGCG CACGGCAGCG AGCTGCAGAA GAAGAAGTTC CTGCCGGCGA TCCTGGCCGG TGAGGCGCAC TTCGCGATCG GCTACACCGA GCCGGAAGCC GGCACCGACC TGGCGTCGTT GCGCACCACC GCCGTTCGCG ACGGCGACCA CTACATCGTC AACGGCCAGA AGGTCTTCAC CACCGGCGCA CACGACGCCG ACTACATCTG GCTGGCCTGC CGCACCGACC CGAATGCCGC TAAGCACAAA GGCATTTCCA TTCTAATCGT CGACACCAAG GATCCCGGCT ATTCCTGGAC GCCGATCATC TTGGCCGACG GCGCCCACCA CACCAATGCC ACGTACTACA ACGACGTGCG CGTGCCGGTC GACATGCTGG TCGGAAAGGA GAACGACGGC TGGCGGCTGA TCACCACCCA ACTCAACAAC GAACGGGTAA TGCTCGGCCC GGCCGGGCGG TTCGCCAGCA TCTACGACCG GGTGCACGCG TGGGCGTCCG TGCCGGGTGG CAACGGCGTG ACGCCGATCG ACCACGACGA CGTCAAGCGG GCTCTTGGTG AGATTCGTGC GATCTGGCGG ATCAACGAGT TGCTCAACTG GCAGGTAGCG TCCGCCGGTG AGGACATCAA CATGGCCGAT GCCGCGGCCA CGAAAGTCTT TGGCACCGAG CGTGTTCAGC GTGCCGGCCG GCTCGCCGAG GAAATCGTTG GCAAGTACGG CAACCCCGCC GAACCCGACA CCGCCGAGCT ACTGCGCTGG CTGGACGCCC AGACCAAGCG CAACCTGGTG ATCACCTTCG GCGGGGGTGT GAACGAAGTC ATGCGCGAGA TGATCGCCGC GTCTGGCCTC AAAGTGCCAA GGGTGCCCAG G
Details for MytuD.01117.a.A1.GE25933
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 44kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFIDLTPEQ RQLQAEIRQY FSNLISPDER TEMEKDRHGP AYRAVIRRMG RDGRLGVGWP KEFGGLGFGP IEQQIFVNEA HRADVPLPAV TLQTVGPTLQ AHGSELQKKK FLPAILAGEA HFAIGYTEPE AGTDLASLRT TAVRDGDHYI VNGQKVFTTG AHDADYIWLA CRTDPNAAKH KGISILIVDT KDPGYSWTPI ILADGAHHTN ATYYNDVRVP VDMLVGKEND GWRLITTQLN NERVMLGPAG RFASIYDRVH AWASVPGGNG VTPIDHDDVK RALGEIRAIW RINELLNWQV ASAGEDINMA DAAATKVFGT ERVQRAGRLA EEIVGKYGNP AEPDTAELLR WLDAQTKRNL VITFGGGVNE VMREMIAASG LKVPRVPR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca ttgacctgac gccggagcag cgccagctgc aagccgagat acggcaatac ttttcgaatc tcatctcgcc cgacgagcgc acggagatgg agaaagaccg gcacggcccg gcctaccgtg cggtgatacg gcgcatgggc cgggacggca ggctcggtgt tgggtggccg aaagagttcg gcggtctggg ttttggtccg atcgagcagc agatcttcgt caacgaggcc catcgcgccg acgtgccgct gcccgcggtg acgctgcaga ccgtcggccc cacgctgcag gcgcacggca gcgagctgca gaagaagaag ttcctgccgg cgatcctggc cggtgaggcg cacttcgcga tcggctacac cgagccggaa gccggcaccg acctggcgtc gttgcgcacc accgccgttc gcgacggcga ccactacatc gtcaacggcc agaaggtctt caccaccggc gcacacgacg ccgactacat ctggctggcc tgccgcaccg acccgaatgc cgctaagcac aaaggcattt ccattctaat cgtcgacacc aaggatcccg gctattcctg gacgccgatc atcttggccg acggcgccca ccacaccaat gccacgtact acaacgacgt gcgcgtgccg gtcgacatgc tggtcggaaa ggagaacgac ggctggcggc tgatcaccac ccaactcaac aacgaacggg taatgctcgg cccggccggg cggttcgcca gcatctacga ccgggtgcac gcgtgggcgt ccgtgccggg tggcaacggc gtgacgccga tcgaccacga cgacgtcaag cgggctcttg gtgagattcg tgcgatctgg cggatcaacg agttgctcaa ctggcaggta gcgtccgccg gtgaggacat caacatggcc gatgccgcgg ccacgaaagt ctttggcacc gagcgtgttc agcgtgccgg ccggctcgcc gaggaaatcg ttggcaagta cggcaacccc gccgaacccg acaccgccga gctactgcgc tggctggacg cccagaccaa gcgcaacctg gtgatcacct tcggcggggg tgtgaacgaa gtcatgcgcg agatgatcgc cgcgtctggc ctcaaagtgc caagggtgcc caggtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFIDLTPEQ RQLQAEIRQY FSNLISPDER TEMEKDRHGP AYRAVIRRMG RDGRLGVGWP KEFGGLGFGP IEQQIFVNEA HRADVPLPAV TLQTVGPTLQ AHGSELQKKK FLPAILAGEA HFAIGYTEPE AGTDLASLRT TAVRDGDHYI VNGQKVFTTG AHDADYIWLA CRTDPNAAKH KGISILIVDT KDPGYSWTPI ILADGAHHTN ATYYNDVRVP VDMLVGKEND GWRLITTQLN NERVMLGPAG RFASIYDRVH AWASVPGGNG VTPIDHDDVK RALGEIRAIW RINELLNWQV ASAGEDINMA DAAATKVFGT ERVQRAGRLA EEIVGKYGNP AEPDTAELLR WLDAQTKRNL VITFGGGVNE VMREMIAASG LKVPRVPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcattgac ctgacgccgg agcagcgcca gctgcaagcc gagatacggc aatacttttc gaatctcatc tcgcccgacg agcgcacgga gatggagaaa gaccggcacg gcccggccta ccgtgcggtg atacggcgca tgggccggga cggcaggctc ggtgttgggt ggccgaaaga gttcggcggt ctgggttttg gtccgatcga gcagcagatc ttcgtcaacg aggcccatcg cgccgacgtg ccgctgcccg cggtgacgct gcagaccgtc ggccccacgc tgcaggcgca cggcagcgag ctgcagaaga agaagttcct gccggcgatc ctggccggtg aggcgcactt cgcgatcggc tacaccgagc cggaagccgg caccgacctg gcgtcgttgc gcaccaccgc cgttcgcgac ggcgaccact acatcgtcaa cggccagaag gtcttcacca ccggcgcaca cgacgccgac tacatctggc tggcctgccg caccgacccg aatgccgcta agcacaaagg catttccatt ctaatcgtcg acaccaagga tcccggctat tcctggacgc cgatcatctt ggccgacggc gcccaccaca ccaatgccac gtactacaac gacgtgcgcg tgccggtcga catgctggtc ggaaaggaga acgacggctg gcggctgatc accacccaac tcaacaacga acgggtaatg ctcggcccgg ccgggcggtt cgccagcatc tacgaccggg tgcacgcgtg ggcgtccgtg ccgggtggca acggcgtgac gccgatcgac cacgacgacg tcaagcgggc tcttggtgag attcgtgcga tctggcggat caacgagttg ctcaactggc aggtagcgtc cgccggtgag gacatcaaca tggccgatgc cgcggccacg aaagtctttg gcaccgagcg tgttcagcgt gccggccggc tcgccgagga aatcgttggc aagtacggca accccgccga acccgacacc gccgagctac tgcgctggct ggacgcccag accaagcgca acctggtgat caccttcggc gggggtgtga acgaagtcat gcgcgagatg atcgccgcgt ctggcctcaa agtgccaagg gtgcccagga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a