MyavA.01117.b

Putative acyl-CoA dehydrogenase

CENTER ID: MyavA.01117.b
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01117.b.A1.GE29058 Full length( MyavA.01117.b ) 1 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01117.b.A1.PW28963 Full length( MyavA.01117.b ) 1 397

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_145209
PATRIC ID: fig|243243.7.peg.1557
RefSeq: YP_880829.1
UniProt: A0A0H2ZVD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFSRVTLSD EDQRFLDEAR EFLRTHVTDE VRRRDRETGD NFDEGVHLAL GAAGYLEREW KPEAEGGFSR VQRRIWELEK RRAHVPWVTW GTTAMVARSV AKFGSEELQR EVLPGVFSGH VRLCLGYTEP EGGSDVATCK TRAVRDGNGW IINGSKMFTT GAHNCQYVFL ITNTDPDAPK HKSLTMFLVP LDSPGIDIQG IRTVDGDRTN IVYYSDVRVD DKYRLGDVNA GWTVLREPLN VEHGAVAAAP DGLQDVSIMM HQAGFMADAL DKVAAKVTLP DPHGRRLIDD SAIAYRLGRN AARMEAALSA PSIYGRVALA QTMRDISPDL MDILGTASAL PVGTDGAADD GASEYVFRFA PLVGIYGGTL EVFRNMIAQY MLGLGKPAYA PVAQRAS
NT Sequence
GTGGACTTCT CACGTGTCAC GCTGTCCGAC GAGGACCAGC GATTCCTGGA CGAGGCTCGC GAGTTCCTGC GCACCCATGT GACCGACGAG GTCAGGCGCC GGGACCGGGA AACCGGCGAC AACTTCGACG AGGGCGTGCA CCTGGCGTTG GGTGCGGCCG GCTATCTGGA ACGCGAATGG AAGCCCGAGG CCGAGGGCGG TTTCAGCCGG GTGCAACGTC GCATCTGGGA GCTGGAGAAA CGACGCGCCC ACGTGCCCTG GGTGACCTGG GGGACCACCG CCATGGTGGC CCGCTCGGTG GCCAAGTTCG GGTCCGAGGA GTTACAGCGG GAAGTGCTGC CCGGCGTGTT CAGCGGGCAC GTGCGGCTGT GCCTGGGCTA TACCGAACCC GAGGGCGGAT CCGATGTGGC CACCTGCAAG ACCAGGGCGG TCCGGGACGG CAACGGCTGG ATCATCAACG GCTCCAAGAT GTTCACCACC GGGGCGCACA ACTGCCAATA CGTTTTCCTG ATCACCAACA CCGACCCCGA TGCGCCGAAG CACAAGAGCC TGACCATGTT CCTGGTGCCG CTGGACTCGC CGGGCATCGA CATCCAGGGC ATCCGCACCG TCGACGGGGA CCGCACCAAC ATCGTCTACT ACAGCGACGT GCGGGTCGAC GACAAGTATC GGCTGGGCGA CGTGAACGCC GGCTGGACCG TGTTGCGCGA GCCGCTCAAC GTCGAGCACG GGGCGGTGGC GGCGGCCCCG GACGGGCTGC AGGACGTGTC GATCATGATG CACCAGGCCG GGTTCATGGC CGACGCCCTG GACAAGGTCG CGGCCAAGGT CACGCTGCCA GACCCGCACG GGCGCAGGCT CATTGACGAT TCGGCGATCG CGTATCGGTT GGGCCGCAAC GCCGCGCGGA TGGAGGCGGC GCTGAGCGCG CCCAGCATCT ACGGACGCGT CGCCCTCGCC CAGACGATGC GCGACATCTC CCCGGACCTG ATGGACATCC TGGGCACGGC GTCCGCGCTG CCGGTCGGCA CCGACGGCGC GGCCGACGAC GGCGCCTCCG AATACGTCTT CCGGTTCGCC CCGCTGGTCG GCATCTACGG CGGCACCCTC GAGGTGTTCC GCAACATGAT CGCGCAGTAC ATGCTCGGCC TGGGCAAGCC GGCCTACGCG CCGGTGGCCC AGCGCGCCAG C
Details for MyavA.01117.b.A1.GE29058
HARVESTED ON: data unavailable
SEQUENCED ON: 4/29/2010
EXPECTED MW: 46kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MVDFSRVTLS DEDQRFLDEA REFLRTHVTD EVRRRDRETG DNFDEGVHLA LGAAGYLERE WKPEAEGGFS RVQRRIWELE KRRAHVPWVT WGTTAMVARS VAKFGSEELQ REVLPGVFSG HVRLCLGYTE PEGGSDVATC KTRAVRDGNG WIVNGSKMFT TGAHNCQYVF LITNTDPDAP KHKSLTMFLV PLDSPGIDIQ GIRTVDGDRT NIVYYSDVRV DDKYRLGDVN AGWTVLREPL NVEHGAVAAA PDGLQDVSIM MHQAGFMADA LDKVAAKVTQ PDPHGRRLID DSAIAYRLGR NAARMEAALS APSIYGRVAL AQTMRDISPD LMDILGTASA LPVGTDGAAD DGASEYVFRF APLVGIYGGT LEVFRNMIAQ YMLGLGKPAY APVAQRAS
Validated NT Sequence
ttattagctg gcgcgctggg ccaccggcgc gtaggccggc ttgcccaggc cgagcatgta ctgcgcgatc atgttgcgga acacctcgag ggtgccgccg tagatgccga ccagcggggc gaaccggaag acgtactcgg aggcgccgtc gtcggccgcg ccgtcggtgc cgaccggcag cgcggacgcc gtgcccagga tgtccatcag atccggggag atgtcgcgca tcgtctgggc gagggcgacg cgtccgtaga tgctgggcgc gctcagcgcc gcctccatcc gcgcggcgtt gcggcccaac cgatacgcga tcgccgagtc gtcaatgagc ctgcgcccgt gcgggtctgg ctgcgtgacc ttggccgcga ccttgtccag ggcgtcggcc atgaacccgg cctggtgcat catgatcgac acgtcctgca gcccgtccgg ggccgccgcc accgccccgt gttcgacgtt gagcggctcg cgcaacacgg tccagccggc gttcacgtcg cccagccgat acttgtcgtc gacccgcacg tcgctgtagt agacgatgtt ggtgcggtcc ccgtcgacgg tgcggatgcc ctggatgtcg atgcccggcg agtccagcgg aaccaggaac atggtcaggc tcttgtgctt cggcgcatcc gggtcggtgt tggtgatcag gaaaacgtat tggcagttgt gcgccccggt ggtgaacatc ttggagccgt tgacgatcca gccgttgccg tcccggacag ccctggtctt gcaggtggcc acatcggatc cgccctcggg ttcggtatag cccaggcaca gccgcacgtg cccgctgaac acgccgggca gcacttcccg ctgtaactcc tcggacccga acttggccac cgagcgggcc accatggcgg tggtccccca ggtcacccag ggcacgtggg cgcgtcgctt ctccagctcc cagatgcgac gttgcacccg gctgaaaccg ccctcggcct cgggcttcca ttcgcgttcc agatagccgg ccgcacccaa cgccaggtgc acgccctcgt cgaagttgtc gccggtttcc cggtcccggc gcctgacctc gtcggtcaca tgggtgcgca ggaactcgcg agcctcgtcc aggaatcgct ggtcctcgtc ggacagcgtg acacgtgaga agtccaccat cgaaccagga ccctgggtct gagcttccag ggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDFSRVTL SDEDQRFLDE AREFLRTHVT DEVRRRDRET GDNFDEGVHL ALGAAGYLER EWKPEAEGGF SRVQRRIWEL EKRRAHVPWV TWGTTAMVAR SVAKFGSEEL QREVLPGVFS GHVRLCLGYT EPEGGSDVAT CKTRAVRDGN GWIINGSKMF TTGAHNCQYV FLITNTDPDA PKHKSLTMFL VPLDSPGIDI QGIRTVDGDR TNIVYYSDVR VDDKYRLGDV NAGWTVLREP LNVEHGAVAA APDGLQDVSI MMHQAGFMAD ALDKVAAKVT LPDPHGRRLI DDSAIAYRLG RNAARMEAAL SAPSIYGRVA LAQTMRDISP DLMDILGTAS ALPVGTDGAA DDGASEYVFR FAPLVGIYGG TLEVFRNMIA QYMLGLGKPA YAPVAQRAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacttc tcacgtgtca cgctgtccga cgaggaccag cgattcctgg acgaggctcg cgagttcctg cgcacccatg tgaccgacga ggtcaggcgc cgggaccggg aaaccggcga caacttcgac gagggcgtgc acctggcgtt gggtgcggcc ggctatctgg aacgcgaatg gaagcccgag gccgagggcg gtttcagccg ggtgcaacgt cgcatctggg agctggagaa acgacgcgcc cacgtgccct gggtgacctg ggggaccacc gccatggtgg cccgctcggt ggccaagttc gggtccgagg agttacagcg ggaagtgctg cccggcgtgt tcagcgggca cgtgcggctg tgcctgggct ataccgaacc cgagggcgga tccgatgtgg ccacctgcaa gaccagggcg gtccgggacg gcaacggctg gatcatcaac ggctccaaga tgttcaccac cggggcgcac aactgccaat acgttttcct gatcaccaac accgaccccg atgcgccgaa gcacaagagc ctgaccatgt tcctggtgcc gctggactcg ccgggcatcg acatccaggg catccgcacc gtcgacgggg accgcaccaa catcgtctac tacagcgacg tgcgggtcga cgacaagtat cggctgggcg acgtgaacgc cggctggacc gtgttgcgcg agccgctcaa cgtcgagcac ggggcggtgg cggcggcccc ggacgggctg caggacgtgt cgatcatgat gcaccaggcc gggttcatgg ccgacgccct ggacaaggtc gcggccaagg tcacgctgcc agacccgcac gggcgcaggc tcattgacga ttcggcgatc gcgtatcggt tgggccgcaa cgccgcgcgg atggaggcgg cgctgagcgc gcccagcatc tacggacgcg tcgccctcgc ccagacgatg cgcgacatct ccccggacct gatggacatc ctgggcacgg cgtccgcgct gccggtcggc accgacggcg cggccgacga cggcgcctcc gaatacgtct tccggttcgc cccgctggtc ggcatctacg gcggcaccct cgaggtgttc cgcaacatga tcgcgcagta catgctcggc ctgggcaagc cggcctacgc gccggtggcc cagcgcgcca gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MyavA.01117.b.A1.PW28963
PURIFICATION DATe: 11/1/2010
CONCENTRATION: 27.22mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MVDFSRVTLS DEDQRFLDEA REFLRTHVTD EVRRRDRETG DNFDEGVHLA LGAAGYLERE WKPEAEGGFS RVQRRIWELE KRRAHVPWVT WGTTAMVARS VAKFGSEELQ REVLPGVFSG HVRLCLGYTE PEGGSDVATC KTRAVRDGNG WIVNGSKMFT TGAHNCQYVF LITNTDPDAP KHKSLTMFLV PLDSPGIDIQ GIRTVDGDRT NIVYYSDVRV DDKYRLGDVN AGWTVLREPL NVEHGAVAAA PDGLQDVSIM MHQAGFMADA LDKVAAKVTQ PDPHGRRLID DSAIAYRLGR NAARMEAALS APSIYGRVAL AQTMRDISPD LMDILGTASA LPVGTDGAAD DGASEYVFRF APLVGIYGGT LEVFRNMIAQ YMLGLGKPAY APVAQRAS
Validated NT Sequence
ttattagctg gcgcgctggg ccaccggcgc gtaggccggc ttgcccaggc cgagcatgta ctgcgcgatc atgttgcgga acacctcgag ggtgccgccg tagatgccga ccagcggggc gaaccggaag acgtactcgg aggcgccgtc gtcggccgcg ccgtcggtgc cgaccggcag cgcggacgcc gtgcccagga tgtccatcag atccggggag atgtcgcgca tcgtctgggc gagggcgacg cgtccgtaga tgctgggcgc gctcagcgcc gcctccatcc gcgcggcgtt gcggcccaac cgatacgcga tcgccgagtc gtcaatgagc ctgcgcccgt gcgggtctgg ctgcgtgacc ttggccgcga ccttgtccag ggcgtcggcc atgaacccgg cctggtgcat catgatcgac acgtcctgca gcccgtccgg ggccgccgcc accgccccgt gttcgacgtt gagcggctcg cgcaacacgg tccagccggc gttcacgtcg cccagccgat acttgtcgtc gacccgcacg tcgctgtagt agacgatgtt ggtgcggtcc ccgtcgacgg tgcggatgcc ctggatgtcg atgcccggcg agtccagcgg aaccaggaac atggtcaggc tcttgtgctt cggcgcatcc gggtcggtgt tggtgatcag gaaaacgtat tggcagttgt gcgccccggt ggtgaacatc ttggagccgt tgacgatcca gccgttgccg tcccggacag ccctggtctt gcaggtggcc acatcggatc cgccctcggg ttcggtatag cccaggcaca gccgcacgtg cccgctgaac acgccgggca gcacttcccg ctgtaactcc tcggacccga acttggccac cgagcgggcc accatggcgg tggtccccca ggtcacccag ggcacgtggg cgcgtcgctt ctccagctcc cagatgcgac gttgcacccg gctgaaaccg ccctcggcct cgggcttcca ttcgcgttcc agatagccgg ccgcacccaa cgccaggtgc acgccctcgt cgaagttgtc gccggtttcc cggtcccggc gcctgacctc gtcggtcaca tgggtgcgca ggaactcgcg agcctcgtcc aggaatcgct ggtcctcgtc ggacagcgtg acacgtgaga agtccaccat cgaaccagga ccctgggtct gagcttccag ggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDFSRVTL SDEDQRFLDE AREFLRTHVT DEVRRRDRET GDNFDEGVHL ALGAAGYLER EWKPEAEGGF SRVQRRIWEL EKRRAHVPWV TWGTTAMVAR SVAKFGSEEL QREVLPGVFS GHVRLCLGYT EPEGGSDVAT CKTRAVRDGN GWIINGSKMF TTGAHNCQYV FLITNTDPDA PKHKSLTMFL VPLDSPGIDI QGIRTVDGDR TNIVYYSDVR VDDKYRLGDV NAGWTVLREP LNVEHGAVAA APDGLQDVSI MMHQAGFMAD ALDKVAAKVT LPDPHGRRLI DDSAIAYRLG RNAARMEAAL SAPSIYGRVA LAQTMRDISP DLMDILGTAS ALPVGTDGAA DDGASEYVFR FAPLVGIYGG TLEVFRNMIA QYMLGLGKPA YAPVAQRAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGACTTC TCACGTGTCA CGCTGTCCGA CGAGGACCAG CGATTCCTGG ACGAGGCTCG CGAGTTCCTG CGCACCCATG TGACCGACGA GGTCAGGCGC CGGGACCGGG AAACCGGCGA CAACTTCGAC GAGGGCGTGC ACCTGGCGTT GGGTGCGGCC GGCTATCTGG AACGCGAATG GAAGCCCGAG GCCGAGGGCG GTTTCAGCCG GGTGCAACGT CGCATCTGGG AGCTGGAGAA ACGACGCGCC CACGTGCCCT GGGTGACCTG GGGGACCACC GCCATGGTGG CCCGCTCGGT GGCCAAGTTC GGGTCCGAGG AGTTACAGCG GGAAGTGCTG CCCGGCGTGT TCAGCGGGCA CGTGCGGCTG TGCCTGGGCT ATACCGAACC CGAGGGCGGA TCCGATGTGG CCACCTGCAA GACCAGGGCG GTCCGGGACG GCAACGGCTG GATCATCAAC GGCTCCAAGA TGTTCACCAC CGGGGCGCAC AACTGCCAAT ACGTTTTCCT GATCACCAAC ACCGACCCCG ATGCGCCGAA GCACAAGAGC CTGACCATGT TCCTGGTGCC GCTGGACTCG CCGGGCATCG ACATCCAGGG CATCCGCACC GTCGACGGGG ACCGCACCAA CATCGTCTAC TACAGCGACG TGCGGGTCGA CGACAAGTAT CGGCTGGGCG ACGTGAACGC CGGCTGGACC GTGTTGCGCG AGCCGCTCAA CGTCGAGCAC GGGGCGGTGG CGGCGGCCCC GGACGGGCTG CAGGACGTGT CGATCATGAT GCACCAGGCC GGGTTCATGG CCGACGCCCT GGACAAGGTC GCGGCCAAGG TCACGCTGCC AGACCCGCAC GGGCGCAGGC TCATTGACGA TTCGGCGATC GCGTATCGGT TGGGCCGCAA CGCCGCGCGG ATGGAGGCGG CGCTGAGCGC GCCCAGCATC TACGGACGCG TCGCCCTCGC CCAGACGATG CGCGACATCT CCCCGGACCT GATGGACATC CTGGGCACGG CGTCCGCGCT GCCGGTCGGC ACCGACGGCG CGGCCGACGA CGGCGCCTCC GAATACGTCT TCCGGTTCGC CCCGCTGGTC GGCATCTACG GCGGCACCCT CGAGGTGTTC CGCAACATGA TCGCGCAGTA CATGCTCGGC CTGGGCAAGC CGGCCTACGC GCCGGTGGCC CAGCGCGCCA GCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA