MythA.01117.b

Putative acyl-CoA dehydrogenase (A0R4Y1 homolog)

CENTER ID: MythA.01117.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.01117.b.A1.GE28770 Full length( MythA.01117.b ) 1 409
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1078020.3.peg.2821
RefSeq: WP_003926351.1
UniProt: G7CGX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARRGVWIAS GAWIWRMPPW AAGGQGTTGT EMYIELTPEQ KKLQAELREY FANLISPEER RAMEVDRHNE AYRAVIRRMG ADGKLGVGWP KEFGGLGYGP VEQSIFVNEA ARADVPLPAV TLQTVGPTLQ VYGTEEQKKK FLPAILAGEV HFAIGYTEPE AGTDLASLRT TAVRRKEGSA EYYLVNGQKV FTTGAHDADY IWLACRTDPN AAKHKGISIL IVDTKDPGYS WTPMILSDGA HHTNATYYND VRVPADMLVG EENGGWKLIT TQLNHERVML GPAGKVAGIY DKVHAWATKP GPSGGAPIDD ANVRRILGEI RAIWRINELL NWQGPXXXXX XXXXXXXXXX XXPPRCSPPS GFRRSAGSPR RSSADTATRP IRRPPNCWTG WTSRPNATSS SPSVAGSTR
NT Sequence
GTGGCCCGTC GCGGTGTCTG GATCGCCTCG GGGGCCTGGA TCTGGCGGAT GCCGCCGTGG GCGGCCGGGG GCCAGGGGAC GACGGGAACT GAGATGTACA TCGAACTGAC TCCGGAACAG AAGAAACTGC AGGCCGAACT GCGCGAATAC TTCGCCAACC TCATCTCGCC CGAGGAGCGC AGGGCGATGG AGGTCGACCG GCACAACGAG GCCTACCGCG CGGTGATCCG GCGGATGGGC GCCGACGGCA AGCTCGGTGT GGGGTGGCCC AAGGAGTTCG GCGGGCTCGG TTACGGCCCG GTCGAGCAGA GCATCTTCGT CAATGAGGCG GCCCGGGCGG ATGTCCCGCT GCCGGCGGTG ACCCTGCAGA CGGTCGGCCC GACGCTGCAG GTGTACGGCA CCGAGGAACA GAAGAAGAAG TTCCTGCCCG CGATCCTGGC CGGTGAAGTG CACTTCGCGA TCGGCTACAC CGAACCCGAG GCTGGCACCG ACCTGGCGTC GCTGCGCACC ACCGCCGTGC GGCGAAAGGA GGGGTCTGCC GAGTATTACC TGGTCAACGG GCAGAAGGTG TTCACCACCG GTGCCCACGA CGCAGACTAC ATCTGGCTGG CCTGCCGTAC CGACCCGAAT GCGGCCAAGC ACAAGGGCAT TTCGATCCTG ATCGTCGACA CCAAGGATCC GGGCTACTCC TGGACGCCGA TGATCCTGTC CGACGGAGCC CACCACACCA ACGCCACCTA CTACAACGAC GTGCGCGTCC CGGCCGACAT GCTCGTCGGC GAGGAGAACG GTGGCTGGAA GCTGATCACC ACCCAGCTCA ACCACGAGCG GGTGATGCTG GGGCCGGCGG GCAAGGTGGC CGGGATCTAC GACAAGGTGC ACGCCTGGGC GACCAAACCC GGCCCCTCCG GCGGGGCCCC GATCGACGAC GCCAACGTGC GCCGGATCCT TGGTGAGATC CGGGCGATCT GGCGGATCAA CGAACTGCTC AACTGGCAGG GCCCCCNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGCCAC CAAGGTGTTC TCCACCGAGC GGATTCAGAA GGTCGGCCGG CTCGCCGAGG AGATCGTCGG CGGATACGGC GACCCGGCCG ATCCGGAGAC CGCCGAACTG CTGGACTGGC TGGACAAGCA GACCAAACGC AACCTCGTCA TCACCTTCGG TGGCGGGGTC AACGAGG
Details for MythA.01117.b.A1.GE28770
HARVESTED ON: 3/1/2010
SEQUENCED ON: 5/19/2010
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 85
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVARRGVWI ASGAWIWRMP PWAAGGQGTT GTEMYIELTP EQKKLQAELR EYFANLISPE ERRAMEVDRH NEAYRAVIRR MGADGKLGVG WPKEFGGLGY GPVEQSIFVN EAARADVPLP AVTLQTVGPT LQVYGTEEQK KKFLPAILAG EVHFAIGYTE PEAGTDLASL RTTAVRRKEG SAEYYLVNGQ KVFTTGAHDA DYIWLACRTD PNAAKHKGIS ILIVDTKDPG YSWTPMILSD GAHHTNATYY NDVRVPADML VGEENGGWKL ITTQLNHERV MLGPAGKVAG IYDKVHAWAT KPGPSGGAPI DDANVRRILG EIRAIWRINE LLNWQVAAAG ETIAVADAAA TKVFSTERIQ KVGRLAEEIV GGYGDPADPE TAELLDWLDK QTKRNLVITF GGGVNEVI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg cccgtcgcgg tgtctggatc gcctcggggg cctggatctg gcggatgccg ccgtgggcgg ccgggggcca ggggacgacg ggaactgaga tgtacatcga actgactccg gaacagaaga aactgcaggc cgaactgcgc gaatacttcg ccaacctcat ctcgcccgag gagcgcaggg cgatggaggt cgaccggcac aacgaggcct accgcgcggt gatccggcgg atgggcgccg acggcaagct cggtgtgggg tggcccaagg agttcggcgg gctcggttac ggcccggtcg agcagagcat cttcgtcaat gaggcggccc gggcggatgt cccgctgccg gcggtgaccc tgcagacggt cggcccgacg ctgcaggtgt acggcaccga ggaacagaag aagaagttcc tgcccgcgat cctggccggt gaagtgcact tcgcgatcgg ctacaccgaa cccgaggctg gcaccgacct ggcgtcgctg cgcaccaccg ccgtgcggcg aaaggagggg tctgccgagt attacctggt caacgggcag aaggtgttca ccaccggtgc ccacgacgca gactacatct ggctggcctg ccgtaccgac ccgaatgcgg ccaagcacaa gggcatttcg atcctgatcg tcgacaccaa ggatccgggc tactcctgga cgccgatgat cctgtccgac ggagcccacc acaccaacgc cacctactac aacgacgtgc gcgtcccggc cgacatgctc gtcggcgagg agaacggtgg ctggaagctg atcaccaccc agctcaacca cgagcgggtg atgctggggc cggcgggcaa ggtggccggg atctacgaca aggtgcacgc ctgggcgacc aaacccggcc cctccggcgg ggccccgatc gacgacgcca acgtgcgccg gatccttggt gagatccggg cgatctggcg gatcaacgaa ctgctcaact ggcaggtcgc cgcggccggt gagacgatcg ccgtcgccga cgcggccgcc accaaggtgt tctccaccga gcggattcag aaggtcggcc ggctcgccga ggagatcgtc ggcggatacg gcgacccggc cgatccggag accgccgaac tgctggactg gctggacaag cagaccaaac gcaacctcgt catcaccttc ggtggcgggg tcaacgaggt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVARRGVWI ASGAWIWRMP PWAAGGQGTT GTEMYIELTP EQKKLQAELR EYFANLISPE ERRAMEVDRH NEAYRAVIRR MGADGKLGVG WPKEFGGLGY GPVEQSIFVN EAARADVPLP AVTLQTVGPT LQVYGTEEQK KKFLPAILAG EVHFAIGYTE PEAGTDLASL RTTAVRRKEG SAEYYLVNGQ KVFTTGAHDA DYIWLACRTD PNAAKHKGIS ILIVDTKDPG YSWTPMILSD GAHHTNATYY NDVRVPADML VGEENGGWKL ITTQLNHERV MLGPAGKVAG IYDKVHAWAT KPGPSGGAPI DDANVRRILG EIRAIWRINE LLNWQGPXXX XXXXXXXXXX XXXXPPRCSP PSGFRRSAGS PRRSSADTAT RPIRRPPNCW TGWTSRPNAT SSSPSVAGST R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcccgt cgcggtgtct ggatcgcctc gggggcctgg atctggcgga tgccgccgtg ggcggccggg ggccagggga cgacgggaac tgagatgtac atcgaactga ctccggaaca gaagaaactg caggccgaac tgcgcgaata cttcgccaac ctcatctcgc ccgaggagcg cagggcgatg gaggtcgacc ggcacaacga ggcctaccgc gcggtgatcc ggcggatggg cgccgacggc aagctcggtg tggggtggcc caaggagttc ggcgggctcg gttacggccc ggtcgagcag agcatcttcg tcaatgaggc ggcccgggcg gatgtcccgc tgccggcggt gaccctgcag acggtcggcc cgacgctgca ggtgtacggc accgaggaac agaagaagaa gttcctgccc gcgatcctgg ccggtgaagt gcacttcgcg atcggctaca ccgaacccga ggctggcacc gacctggcgt cgctgcgcac caccgccgtg cggcgaaagg aggggtctgc cgagtattac ctggtcaacg ggcagaaggt gttcaccacc ggtgcccacg acgcagacta catctggctg gcctgccgta ccgacccgaa tgcggccaag cacaagggca tttcgatcct gatcgtcgac accaaggatc cgggctactc ctggacgccg atgatcctgt ccgacggagc ccaccacacc aacgccacct actacaacga cgtgcgcgtc ccggccgaca tgctcgtcgg cgaggagaac ggtggctgga agctgatcac cacccagctc aaccacgagc gggtgatgct ggggccggcg ggcaaggtgg ccgggatcta cgacaaggtg cacgcctggg cgaccaaacc cggcccctcc ggcggggccc cgatcgacga cgccaacgtg cgccggatcc ttggtgagat ccgggcgatc tggcggatca acgaactgct caactggcag ggcccccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcca ccaaggtgtt ctccaccgag cggattcaga aggtcggccg gctcgccgag gagatcgtcg gcggatacgg cgacccggcc gatccggaga ccgccgaact gctggactgg ctggacaagc agaccaaacg caacctcgtc atcaccttcg gtggcggggt caacgaggaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa