MypaA.01117.e

FadE29

CENTER ID: MypaA.01117.e
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01117.e.A1.GE29550 Full length( MypaA.01117.e ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01117.e.A1.PS01014 Full length( MypaA.01117.e ) 1 387

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_239169
PATRIC ID: fig|262316.17.peg.555
RefSeq: NP_959458.1
UniProt: Q743R7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIDLTPEQR QLQAELREYF SNLISSDEMK AMEQDRHNEA YRAVIRRMGR DGKLGVGWPK EFGGLGFGPI EQSIFVNEAH RADVPLPAVT LQTVGPTLQQ FGSEMQKKKF LPAILAGEVH FAIGYTEPEA GTDLASLRTT AVRQGDEYIV NGQKIFTTGA HDADYIWLAC RTDPEAVKHK GISILIVDTK DPGYSWTPII LSDGAHHTNA TYYNDVRVPA DMLVGEENGG WKLITTQLNN ERVMLGPAGR TAGIYDRVRE WASKPGSDGV TPIDHADVQR ALGEIYAMWR INELLNWQVA AAGEDINVAD AASTKVFGTE RIQYIGRLAE EVVGKYGNPA DPETGELLRW LDSQTKRNLV ITFGGGVNEV MREMIAAAGL KVPRVPR
NT Sequence
ATGTTCATAG ACCTGACCCC CGAACAGCGT CAGCTGCAAG CCGAACTGCG GGAATACTTT TCGAACCTGA TCTCGTCCGA CGAGATGAAG GCGATGGAGC AGGACCGCCA CAACGAGGCC TACCGGGCGG TGATCCGGCG GATGGGCCGG GACGGCAAGC TCGGGGTGGG CTGGCCAAAG GAGTTCGGCG GCTTGGGCTT CGGCCCGATC GAGCAGTCGA TCTTCGTCAA CGAGGCGCAT CGCGCCGACG TGCCGCTGCC CGCGGTGACC CTGCAGACGG TCGGGCCGAC GCTGCAACAG TTCGGCAGCG AGATGCAGAA GAAGAAGTTC CTGCCGGCGA TCCTGGCCGG CGAGGTGCAC TTCGCGATCG GCTACACCGA GCCGGAGGCC GGCACCGACC TGGCGTCGCT GCGCACCACG GCCGTGCGGC AGGGCGACGA GTACATCGTC AACGGGCAGA AGATCTTCAC CACCGGTGCC CATGACGCCG ACTACATCTG GCTGGCCTGC CGCACCGATC CGGAAGCCGT GAAGCACAAG GGCATTTCGA TCCTGATCGT GGACACCAAG GACCCCGGCT ACTCGTGGAC GCCGATCATC CTGTCCGACG GCGCCCACCA CACCAACGCC ACCTACTACA ACGACGTGCG GGTGCCCGCC GACATGCTGG TCGGCGAGGA GAACGGCGGG TGGAAGCTGA TCACCACCCA GCTCAACAAC GAGCGGGTGA TGCTGGGCCC GGCCGGCCGT ACCGCCGGCA TCTACGACAG GGTGCGCGAA TGGGCGTCCA AGCCGGGCAG CGACGGGGTC ACCCCGATCG ACCACGCCGA CGTCCAGCGG GCGCTCGGTG AGATCTATGC GATGTGGCGG ATCAACGAGT TGCTCAACTG GCAGGTGGCT GCGGCCGGTG AGGACATCAA CGTCGCCGAC GCCGCGTCGA CGAAAGTCTT TGGCACCGAG CGGATTCAGT ACATCGGCCG GCTCGCCGAG GAAGTCGTCG GCAAGTACGG GAACCCCGCC GACCCGGAGA CCGGCGAACT GCTCAGGTGG CTGGACTCGC AAACCAAGCG CAACCTGGTG ATCACGTTCG GCGGCGGTGT CAACGAAGTG ATGCGCGAGA TGATCGCCGC CGCCGGGCTC AAAGTGCCGA GGGTGCCCCG A
Details for MypaA.01117.e.A1.GE29550
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 69
Validated AA Sequence
MFIDLTPEQR QLQAELREYF SNLISSDEMK AMEQDRHNEA YRAVIRRMGR DGKLGVGWPK EFGGLGFGPI EQSIFVNEAH RADVPLPAVT LQTVGPTLQQ FGSEMQKKKF LPAILAGEVH FAIGYTEPEA GTDLASLRTT AVRQGDEYIV NGQKIFTTGA HDADYIWLAC RTDPEAVKHK GISILIVDTK DPGYSWTPII LSDGAHHTNA TYYNDVRVPA DMLVGEENGG WKLITTQLNN ERVMLGPGRP YRRHLRQGAR MGVQAGQRRG HPDRPRRRPA GAR
Validated NT Sequence
ggggcaccct cggcactttg agcccggcgg cggcgatcat ctcgcgcatc acttcgttga caccgccgcc gaacgtgatc accaggttgc gcttggtttg cgagtccagc cacctgagca nntnnnncgg tctccgggtc ggcggggttc ccgtacttgc cgacgacttc ctcggcgagc cggccgatgt actgaatccg ctcggtgcca aagactttcg tcgacgcggc gtcggcgacg ttgatgtcct caccggccgc agccacctgc cagttgagca actcgttgat ccgccacatc gcatagatct caccgagcgc ccgctggacg tcggcgtggt cgatcggggt gaccccgtcg ctgcccggct tggacgccca ttcgcgcacc ctgtcgtaga tgccggcggt acggccggcc ngggcccagc atcacccgct cgttgttgag ctgggtggtg atcagcttcc acccgccgtt ctcctcgccg accagcatgt cggcgggcac ccgcacgtcg ttgtagtagg tggcgttggt gtggtgggcg ccgtcggaca ggatgatcgg cgtccacgag tagccggggt ccttggtgtc cacgatcagg atcgaaatgc ccttgtgctt cacggcttcc ggatcggtgc ggcaggccag ccagatgtag tcggcgtcat gggcaccggt ggtgaagatc ttctgcccgt tgacgatgta ctcgtcgccc tgccgcacgg ccgtggtgcg cagcgacgcc aggtcggtgc cggcctccgg ctcggtgtag ccgatcgcga agtgcacctc gccggccagg atcgccggca ggaacttctt cttctgcatc tcgctgccga actgttgcag cgtcggcccg accgtctgca gggtcaccgc gggcagcggc acgtcggcgc gatgcgcctc gttgacgaag atcgactgct cgatcgggcc gaagcccaag ccgccgaact cctttggcca gcccaccccg agcttgccgt cccggcccat ccgccggatc accgcccggt aggcctcgtt gtggcggtcc tgctccatcg ccttcatctc gtcggacgag atcaggttcg aaaagtattc ccgcagttcg gcttgcagct gacgctgttc gggggtcagg tctatgaaca tcgaaccagg accctgggtc tgagcttcca gggtaccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFIDLTPEQ RQLQAELREY FSNLISSDEM KAMEQDRHNE AYRAVIRRMG RDGKLGVGWP KEFGGLGFGP IEQSIFVNEA HRADVPLPAV TLQTVGPTLQ QFGSEMQKKK FLPAILAGEV HFAIGYTEPE AGTDLASLRT TAVRQGDEYI VNGQKIFTTG AHDADYIWLA CRTDPEAVKH KGISILIVDT KDPGYSWTPI ILSDGAHHTN ATYYNDVRVP ADMLVGEENG GWKLITTQLN NERVMLGPAG RTAGIYDRVR EWASKPGSDG VTPIDHADVQ RALGEIYAMW RINELLNWQV AAAGEDINVA DAASTKVFGT ERIQYIGRLA EEVVGKYGNP ADPETGELLR WLDSQTKRNL VITFGGGVNE VMREMIAAAG LKVPRVPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcatagac ctgacccccg aacagcgtca gctgcaagcc gaactgcggg aatacttttc gaacctgatc tcgtccgacg agatgaaggc gatggagcag gaccgccaca acgaggccta ccgggcggtg atccggcgga tgggccggga cggcaagctc ggggtgggct ggccaaagga gttcggcggc ttgggcttcg gcccgatcga gcagtcgatc ttcgtcaacg aggcgcatcg cgccgacgtg ccgctgcccg cggtgaccct gcagacggtc gggccgacgc tgcaacagtt cggcagcgag atgcagaaga agaagttcct gccggcgatc ctggccggcg aggtgcactt cgcgatcggc tacaccgagc cggaggccgg caccgacctg gcgtcgctgc gcaccacggc cgtgcggcag ggcgacgagt acatcgtcaa cgggcagaag atcttcacca ccggtgccca tgacgccgac tacatctggc tggcctgccg caccgatccg gaagccgtga agcacaaggg catttcgatc ctgatcgtgg acaccaagga ccccggctac tcgtggacgc cgatcatcct gtccgacggc gcccaccaca ccaacgccac ctactacaac gacgtgcggg tgcccgccga catgctggtc ggcgaggaga acggcgggtg gaagctgatc accacccagc tcaacaacga gcgggtgatg ctgggcccgg ccggccgtac cgccggcatc tacgacaggg tgcgcgaatg ggcgtccaag ccgggcagcg acggggtcac cccgatcgac cacgccgacg tccagcgggc gctcggtgag atctatgcga tgtggcggat caacgagttg ctcaactggc aggtggctgc ggccggtgag gacatcaacg tcgccgacgc cgcgtcgacg aaagtctttg gcaccgagcg gattcagtac atcggccggc tcgccgagga agtcgtcggc aagtacggga accccgccga cccggagacc ggcgaactgc tcaggtggct ggactcgcaa accaagcgca acctggtgat cacgttcggc ggcggtgtca acgaagtgat gcgcgagatg atcgccgccg ccgggctcaa agtgccgagg gtgccccgaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MypaA.01117.e.A1.PS01014
PURIFICATION DATe: 2/11/2011
CONCENTRATION: 8.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 90
PERCENT COVERAGE: 69
Protocol Notes
notes unavailable
Validated AA Sequence
MFIDLTPEQR QLQAELREYF SNLISSDEMK AMEQDRHNEA YRAVIRRMGR DGKLGVGWPK EFGGLGFGPI EQSIFVNEAH RADVPLPAVT LQTVGPTLQQ FGSEMQKKKF LPAILAGEVH FAIGYTEPEA GTDLASLRTT AVRQGDEYIV NGQKIFTTGA HDADYIWLAC RTDPEAVKHK GISILIVDTK DPGYSWTPII LSDGAHHTNA TYYNDVRVPA DMLVGEENGG WKLITTQLNN ERVMLGPGRP YRRHLRQGAR MGVQAGQRRG HPDRPRRRPA GAR
Validated NT Sequence
ggggcaccct cggcactttg agcccggcgg cggcgatcat ctcgcgcatc acttcgttga caccgccgcc gaacgtgatc accaggttgc gcttggtttg cgagtccagc cacctgagca nntnnnncgg tctccgggtc ggcggggttc ccgtacttgc cgacgacttc ctcggcgagc cggccgatgt actgaatccg ctcggtgcca aagactttcg tcgacgcggc gtcggcgacg ttgatgtcct caccggccgc agccacctgc cagttgagca actcgttgat ccgccacatc gcatagatct caccgagcgc ccgctggacg tcggcgtggt cgatcggggt gaccccgtcg ctgcccggct tggacgccca ttcgcgcacc ctgtcgtaga tgccggcggt acggccggcc ngggcccagc atcacccgct cgttgttgag ctgggtggtg atcagcttcc acccgccgtt ctcctcgccg accagcatgt cggcgggcac ccgcacgtcg ttgtagtagg tggcgttggt gtggtgggcg ccgtcggaca ggatgatcgg cgtccacgag tagccggggt ccttggtgtc cacgatcagg atcgaaatgc ccttgtgctt cacggcttcc ggatcggtgc ggcaggccag ccagatgtag tcggcgtcat gggcaccggt ggtgaagatc ttctgcccgt tgacgatgta ctcgtcgccc tgccgcacgg ccgtggtgcg cagcgacgcc aggtcggtgc cggcctccgg ctcggtgtag ccgatcgcga agtgcacctc gccggccagg atcgccggca ggaacttctt cttctgcatc tcgctgccga actgttgcag cgtcggcccg accgtctgca gggtcaccgc gggcagcggc acgtcggcgc gatgcgcctc gttgacgaag atcgactgct cgatcgggcc gaagcccaag ccgccgaact cctttggcca gcccaccccg agcttgccgt cccggcccat ccgccggatc accgcccggt aggcctcgtt gtggcggtcc tgctccatcg ccttcatctc gtcggacgag atcaggttcg aaaagtattc ccgcagttcg gcttgcagct gacgctgttc gggggtcagg tctatgaaca tcgaaccagg accctgggtc tgagcttcca gggtaccc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFIDLTPEQ RQLQAELREY FSNLISSDEM KAMEQDRHNE AYRAVIRRMG RDGKLGVGWP KEFGGLGFGP IEQSIFVNEA HRADVPLPAV TLQTVGPTLQ QFGSEMQKKK FLPAILAGEV HFAIGYTEPE AGTDLASLRT TAVRQGDEYI VNGQKIFTTG AHDADYIWLA CRTDPEAVKH KGISILIVDT KDPGYSWTPI ILSDGAHHTN ATYYNDVRVP ADMLVGEENG GWKLITTQLN NERVMLGPAG RTAGIYDRVR EWASKPGSDG VTPIDHADVQ RALGEIYAMW RINELLNWQV AAAGEDINVA DAASTKVFGT ERIQYIGRLA EEVVGKYGNP ADPETGELLR WLDSQTKRNL VITFGGGVNE VMREMIAAAG LKVPRVPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCATAGAC CTGACCCCCG AACAGCGTCA GCTGCAAGCC GAACTGCGGG AATACTTTTC GAACCTGATC TCGTCCGACG AGATGAAGGC GATGGAGCAG GACCGCCACA ACGAGGCCTA CCGGGCGGTG ATCCGGCGGA TGGGCCGGGA CGGCAAGCTC GGGGTGGGCT GGCCAAAGGA GTTCGGCGGC TTGGGCTTCG GCCCGATCGA GCAGTCGATC TTCGTCAACG AGGCGCATCG CGCCGACGTG CCGCTGCCCG CGGTGACCCT GCAGACGGTC GGGCCGACGC TGCAACAGTT CGGCAGCGAG ATGCAGAAGA AGAAGTTCCT GCCGGCGATC CTGGCCGGCG AGGTGCACTT CGCGATCGGC TACACCGAGC CGGAGGCCGG CACCGACCTG GCGTCGCTGC GCACCACGGC CGTGCGGCAG GGCGACGAGT ACATCGTCAA CGGGCAGAAG ATCTTCACCA CCGGTGCCCA TGACGCCGAC TACATCTGGC TGGCCTGCCG CACCGATCCG GAAGCCGTGA AGCACAAGGG CATTTCGATC CTGATCGTGG ACACCAAGGA CCCCGGCTAC TCGTGGACGC CGATCATCCT GTCCGACGGC GCCCACCACA CCAACGCCAC CTACTACAAC GACGTGCGGG TGCCCGCCGA CATGCTGGTC GGCGAGGAGA ACGGCGGGTG GAAGCTGATC ACCACCCAGC TCAACAACGA GCGGGTGATG CTGGGCCCGG CCGGCCGTAC CGCCGGCATC TACGACAGGG TGCGCGAATG GGCGTCCAAG CCGGGCAGCG ACGGGGTCAC CCCGATCGAC CACGCCGACG TCCAGCGGGC GCTCGGTGAG ATCTATGCGA TGTGGCGGAT CAACGAGTTG CTCAACTGGC AGGTGGCTGC GGCCGGTGAG GACATCAACG TCGCCGACGC CGCGTCGACG AAAGTCTTTG GCACCGAGCG GATTCAGTAC ATCGGCCGGC TCGCCGAGGA AGTCGTCGGC AAGTACGGGA ACCCCGCCGA CCCGGAGACC GGCGAACTGC TCAGGTGGCT GGACTCGCAA ACCAAGCGCA ACCTGGTGAT CACGTTCGGC GGCGGTGTCA ACGAAGTGAT GCGCGAGATG ATCGCCGCCG CCGGGCTCAA AGTGCCGAGG GTGCCCCGAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A