MyulA.01117.a

Acyl-CoA dehydrogenase FadE29

CENTER ID: MyulA.01117.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01117.a.B1.GE40305 Full length( MyulA.01117.a ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_239169
PATRIC ID: fig|362242.7.peg.4768
RefSeq: YP_907621.1
UniProt: A0PUY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFIDLTPEQR QLQAELRQYF SNLISSDEMK AMEKDRHNAA YRAVIRRMGK DGKLGVGWPK EFGGLGFGPI EQQIFVNEAH RADVPLPAVT LQTVGPTLQA YGSDAQKKKF LPAILAGEVH FAIGYTEPEA GTDLASLRTT AVRQGDEYIV NGQKIFTTGA HDADYIWLAC RTDPEAVKHK GISILIVDTK DLGYSWAPII LSDGAHHTNA TYYNDVRVPA DMLVGEPNGG WRLITTQLNN ERVMLGPAGR FASIYDRVRA WAAKPGGDGV TPIDHDDVRR ALGEIHATWR INELLNWQVA AAGENINVAD AAATKVFGTE RVQRVGRLAE EIVGKYGNPA ESDTAELLEW LDAQTKRNLV ITFGGGVNEV MREMIAAAGL KVPRVPR
NT Sequence
ATGTTCATCG ACCTGACCCC CGAACAGCGT CAGCTGCAAG CCGAACTACG ACAATACTTT TCCAACCTGA TCTCCTCCGA TGAGATGAAG GCGATGGAGA AGGACCGGCA CAACGCGGCA TACCGCGCGG TGATCCGCCG AATGGGCAAA GACGGCAAGC TCGGAGTCGG GTGGCCAAAA GAATTTGGTG GCCTTGGGTT TGGTCCCATC GAGCAACAGA TCTTCGTCAA CGAAGCCCAT CGGGCCGACG TGCCGCTGCC CGCGGTGACG CTGCAGACGG TGGGACCCAC CCTGCAGGCA TACGGCAGCG ACGCGCAGAA AAAGAAATTC CTGCCGGCCA TCCTGGCGGG TGAGGTGCAC TTCGCGATCG GCTACACTGA GCCGGAAGCC GGCACCGACC TGGCATCCCT GCGAACCACG GCGGTACGTC AGGGTGACGA GTACATCGTC AACGGCCAGA AGATCTTCAC CACCGGCGCC CACGATGCCG ACTACATCTG GCTCGCCTGC CGCACCGATC CGGAAGCGGT GAAACACAAA GGCATCTCGA TTCTGATCGT CGACACCAAG GATCTCGGCT ACTCGTGGGC GCCGATCATC CTGTCCGACG GCGCGCACCA CACCAACGCC ACGTACTACA ACGACGTGCG CGTGCCGGCA GACATGTTGG TCGGTGAACC CAACGGCGGC TGGCGCCTGA TCACCACGCA ACTCAACAAC GAGCGGGTGA TGCTGGGCCC GGCCGGCCGA TTCGCCAGCA TCTATGACAG GGTGCGGGCA TGGGCGGCCA AACCGGGTGG GGACGGTGTC ACGCCGATCG ACCACGACGA CGTCAGGCGG GCTCTCGGTG AGATCCACGC GACCTGGCGG ATCAACGAGT TGCTCAATTG GCAGGTGGCC GCCGCGGGTG AGAACATTAA CGTCGCTGAT GCTGCGGCAA CGAAAGTTTT TGGCACCGAG CGTGTTCAGC GAGTTGGCCG GCTCGCCGAA GAAATCGTCG GCAAGTACGG CAACCCTGCC GAGTCTGATA CGGCCGAGCT GCTCGAGTGG CTGGACGCGC AGACCAAACG CAATCTGGTC ATCACCTTCG GCGGAGGGGT TAACGAAGTC ATGCGGGAGA TGATCGCGGC CGCCGGCCTC AAAGTGCCCC GGGTGCCGCG A
Details for MyulA.01117.a.B1.GE40305
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 44kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMF IDLTPEQRQL QAELRQYFSN LISSDEMKAM EKDRHNAAYR AVIRRMGKDG KLGVGWPKEF GGLGFGPIEQ QIFVNEAHRA DVPLPAVTLQ TVGPTLQAYG SDAQKKKFLP AILAGEVHFA IGYTEPEAGT DLASLRTTAV RQGDEYIVNG QKIFTTGAHD ADYIWLACRT DPEAVKHKGI SILIVDTKDL GYSWAPIILS DGAHHTNATY YNDVRVPADM LVGEPNGGWR LITTQLNNER VMLGPAGRFA SIYDRVRAWA AKPGGDGVTP IDHDDVRRAL GEIHATWRIN ELLNWQVAAA GENINVADAA ATKVFGTERV QRVGRLAEEI VGKYGNPAES DTAELLEWLD AQTKRNLVIT FGGGVNEVMR EMIAAAGLKV PRVPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca tcgacctgac ccccgaacag cgtcagctgc aagccgaact acgacaatac ttttccaacc tgatctcctc cgatgagatg aaggcgatgg agaaggaccg gcacaacgcg gcataccgcg cggtgatccg ccgaatgggc aaagacggca agctcggagt cgggtggcca aaagaatttg gtggccttgg gtttggtccc atcgagcaac agatcttcgt caacgaagcc catcgggccg acgtgccgct gcccgcggtg acgctgcaga cggtgggacc caccctgcag gcatacggca gcgacgcgca gaaaaagaaa ttcctgccgg ccatcctggc gggtgaggtg cacttcgcga tcggctacac tgagccggaa gccggcaccg acctggcatc cctgcgaacc acggcggtac gtcagggtga cgagtacatc gtcaacggcc agaagatctt caccaccggc gcccacgatg ccgactacat ctggctcgcc tgccgcaccg atccggaagc ggtgaaacac aaaggcatct cgattctgat cgtcgacacc aaggatctcg gctactcgtg ggcgccgatc atcctgtccg acggcgcgca ccacaccaac gccacgtact acaacgacgt gcgcgtgccg gcagacatgt tggtcggtga acccaacggc ggctggcgcc tgatcaccac gcaactcaac aacgagcggg tgatgctggg cccggccggc cgattcgcca gcatctatga cagggtgcgg gcatgggcgg ccaaaccggg tggggacggt gtcacgccga tcgaccacga cgacgtcagg cgggctctcg gtgagatcca cgcgacctgg cggatcaacg agttgctcaa ttggcaggtg gccgccgcgg gtgagaacat taacgtcgct gatgctgcgg caacgaaagt ttttggcacc gagcgtgttc agcgagttgg ccggctcgcc gaagaaatcg tcggcaagta cggcaaccct gccgagtctg atacggccga gctgctcgag tggctggacg cgcagaccaa acgcaatctg gtcatcacct tcggcggagg ggttaacgaa gtcatgcggg agatgatcgc ggccgccggc ctcaaagtgc cccgggtgcc gcgatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat