MytuD.00906.a

cystathionine gamma-synthase

CENTER ID: MytuD.00906.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00906.a.B1.GE27591 Full length( MytuD.00906.a ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1205
RefSeq: NP_215595.1
TubercuList: Rv1079
MTB Network Portal: Rv1079
UniProt: P9WGB7

Orthologues for Rv1079

Species % Identity % Coverage Clones Proteins Structures
leprae 87 100 1 0 0
marinum 87 100 1 1 0
ulcerans 87 100 1 1 2
paratuberculosis 85 100 1 0 0
smegmatis 80 100 1 1 0
thermoresistibile 80 100 1 0 0
abscessus 77 100 1 1 0
paratuberculosis 40 86 1 0 0
avium 38 97 0 0 0
abscessus 38 86 1 1 0
smegmatis 36 97 0 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEDRTGHQG ISGPATRAIH AGYRPDPATG AVNVPIYASS TFAQDGVGGL RGGFEYARTG NPTRAALEAS LAAVEEGAFA RAFSSGMAAT DCALRAMLRP GDHVVIPDDA YGGTFRLIDK VFTRWDVQYT PVRLADLDAV GAAITPRTRL IWVETPTNPL LSIADITAIA ELGTDRSAKV LVDNTFASPA LQQPLRLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDE EFAFLQNGAG AVPGPFDAYL TMRGLKTLVL RMQRHSENAC AVAEFLADHP SVSSVLYPGL PSHPGHEIAA RQMRGFGGMV SVRMRAGRRA AQDLCAKTRV FILAESLGGV ESLIEHPSAM THASTAGSQL EVPDDLVRLS VGIEDIADLL GDLEQALG
NT Sequence
ATGAGCGAAG ACCGCACGGG ACACCAGGGA ATCAGCGGAC CGGCCACCCG CGCCATCCAC GCTGGCTACC GCCCGGATCC GGCGACCGGG GCGGTGAACG TGCCGATCTA CGCCAGCAGC ACCTTCGCCC AAGACGGCGT CGGCGGTCTG CGTGGCGGTT TCGAATACGC ACGCACCGGC AACCCCACCC GGGCCGCATT GGAGGCCTCG CTGGCGGCAG TCGAGGAGGG TGCTTTCGCG CGGGCATTCA GTTCCGGGAT GGCCGCGACC GACTGCGCCC TGCGGGCGAT GTTACGGCCC GGAGACCACG TCGTCATTCC CGATGACGCC TACGGCGGCA CATTCCGGTT GATAGACAAG GTGTTCACCC GGTGGGATGT CCAGTACACG CCGGTGCGGC TTGCCGATCT GGATGCGGTG GGTGCCGCGA TTACTCCGCG CACCCGGCTG ATTTGGGTGG AGACGCCCAC CAATCCGCTA CTGTCGATCG CCGATATCAC GGCCATTGCC GAGCTGGGCA CAGACAGATC GGCAAAAGTA TTGGTGGACA ATACCTTTGC CTCACCCGCG TTGCAGCAGC CGTTGCGGCT GGGCGCCGAT GTGGTGTTGC ACTCGACTAC CAAGTACATC GGCGGCCATT CCGACGTGGT GGGAGGTGCG CTGGTCACCA ACGACGAAGA GCTGGACGAG GAGTTCGCTT TCTTGCAGAA CGGCGCCGGC GCGGTGCCCG GACCATTCGA CGCCTACCTG ACCATGCGCG GCCTGAAGAC CTTGGTGCTG CGGATGCAGC GGCACAGTGA AAATGCCTGT GCGGTAGCGG AATTCCTCGC TGATCATCCG TCGGTGAGTT CTGTGTTGTA TCCGGGTTTG CCCAGTCATC CCGGGCATGA GATTGCCGCG CGACAGATGC GCGGCTTCGG CGGCATGGTT TCGGTGCGGA TGCGGGCCGG TCGGCGTGCG GCGCAGGACC TGTGTGCCAA GACCCGCGTC TTCATCCTGG CCGAGTCGCT GGGTGGGGTG GAGTCGCTGA TCGAACATCC CAGCGCCATG ACCCATGCGT CGACGGCCGG TTCGCAATTG GAGGTGCCCG ACGATCTGGT GCGGCTTTCG GTCGGTATCG AAGACATTGC CGACCTGCTC GGCGATCTCG AACAGGCCCT GGGT
Details for MytuD.00906.a.B1.GE27591
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MSEDRTGHQG ISGPATRAIH AGYRPDPATG AVNVPIYASS TFAQDGVGGL RGGFEYARTG NPTRAALEAS LAAVEEGAFA RAFSSGMAAT DCALRAMLRP GDHVVIPDDA YGGTFRLIDK VFTRWDVQYT PVRLADLDAV GAAITPRTRL IWVETPTNPL LSIADITAIA ELGTDRSAKV LVDNTFASPA LQQPLRLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDE EFAFLQNGAG AVPGPFDAYL TMRGLKTLVL RMQRHSENAC AVAEFLADHP SVSSVLYPGL PSHPGHEIAA RQMRGFGGMV SVRMRAGRRA AQDLCAKTRV FILAESLGGV ESLIEHPSAM THASTAGSQL EVPDDLVRLS VGIEDIADLL GDLEQALG
Validated NT Sequence
tggctcacca ccaccaccac catatgagcg aagaccgcac gggacaccag ggaatcagcg gaccggccac ccgcgccatc cacgctggct accgcccgga tccggcgacc ggggcggtga acgtgccgat ctacgccagc agcaccttcg cccaagacgg cgtcggcggt ctgcgtggcg gtttcgaata cgcacgcacc ggcaacccca cccgggccgc attggaggcc tcgctggcgg cagtcgagga gggtgctttc gcgcgggcat tcagttccgg gatggccgcg accgactgcg ccctgcgggc gatgttacgg cccggagacc acgtcgtcat tcccgatgac gcctacggcg gcacattccg gttgatagac aaggtgttca cccggtggga tgtccagtac acgccggtgc ggcttgccga tctggatgcg gtgggtgccg cgattactcc gcgcacccgg ctgatttggg tggagacgcc caccaatccg ctactgtcga tcgccgatat cacggccatt gccgagctgg gcacagacag atcggcaaaa gtattggtgg acaatacctt tgcctcaccc gcgttgcagc agccgttgcg gctgggcgcc gatgtggtgt tgcactcgac taccaagtac atcggcggcc attccgacgt ggtgggaggt gcgctggtca ccaacgacga agagctggac gaggagttcg ctttcttgca gaacggcgcc ggcgcggtgc ccggaccatt cgacgcctac ctgaccatgc gcggcctgaa gaccttggtg ctgcggatgc agcggcacag tgaaaatgcc tgtgcggtag cggaattcct cgctgatcat ccgtcggtga gttctgtgtt gtatccgggt ttgcccagtc atcccgggca tgagattgcc gcgcgacaga tgcgcggctt cggcggcatg gtttcggtgc ggatgcgggc cggtcggcgt gcggcgcagg acctgtgtgc caagacccgc gtcttcatcc tggccgagtc gctgggtggg gtggagtcgc tgatcgaaca tcccagcgcc atgacccatg cgtcgacggc cggttcgcaa ttggaggtgc ccgacgatct ggtgcggctt tcggtcggta tcgaagacat tgccgacctg ctcggcgatc tcgaacaggc cctgggttaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMS EDRTGHQGIS GPATRAIHAG YRPDPATGAV NVPIYASSTF AQDGVGGLRG GFEYARTGNP TRAALEASLA AVEEGAFARA FSSGMAATDC ALRAMLRPGD HVVIPDDAYG GTFRLIDKVF TRWDVQYTPV RLADLDAVGA AITPRTRLIW VETPTNPLLS IADITAIAEL GTDRSAKVLV DNTFASPALQ QPLRLGADVV LHSTTKYIGG HSDVVGGALV TNDEELDEEF AFLQNGAGAV PGPFDAYLTM RGLKTLVLRM QRHSENACAV AEFLADHPSV SSVLYPGLPS HPGHEIAARQ MRGFGGMVSV RMRAGRRAAQ DLCAKTRVFI LAESLGGVES LIEHPSAMTH ASTAGSQLEV PDDLVRLSVG IEDIADLLGD LEQALG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg aagaccgcac gggacaccag ggaatcagcg gaccggccac ccgcgccatc cacgctggct accgcccgga tccggcgacc ggggcggtga acgtgccgat ctacgccagc agcaccttcg cccaagacgg cgtcggcggt ctgcgtggcg gtttcgaata cgcacgcacc ggcaacccca cccgggccgc attggaggcc tcgctggcgg cagtcgagga gggtgctttc gcgcgggcat tcagttccgg gatggccgcg accgactgcg ccctgcgggc gatgttacgg cccggagacc acgtcgtcat tcccgatgac gcctacggcg gcacattccg gttgatagac aaggtgttca cccggtggga tgtccagtac acgccggtgc ggcttgccga tctggatgcg gtgggtgccg cgattactcc gcgcacccgg ctgatttggg tggagacgcc caccaatccg ctactgtcga tcgccgatat cacggccatt gccgagctgg gcacagacag atcggcaaaa gtattggtgg acaatacctt tgcctcaccc gcgttgcagc agccgttgcg gctgggcgcc gatgtggtgt tgcactcgac taccaagtac atcggcggcc attccgacgt ggtgggaggt gcgctggtca ccaacgacga agagctggac gaggagttcg ctttcttgca gaacggcgcc ggcgcggtgc ccggaccatt cgacgcctac ctgaccatgc gcggcctgaa gaccttggtg ctgcggatgc agcggcacag tgaaaatgcc tgtgcggtag cggaattcct cgctgatcat ccgtcggtga gttctgtgtt gtatccgggt ttgcccagtc atcccgggca tgagattgcc gcgcgacaga tgcgcggctt cggcggcatg gtttcggtgc ggatgcgggc cggtcggcgt gcggcgcagg acctgtgtgc caagacccgc gtcttcatcc tggccgagtc gctgggtggg gtggagtcgc tgatcgaaca tcccagcgcc atgacccatg cgtcgacggc cggttcgcaa ttggaggtgc ccgacgatct ggtgcggctt tcggtcggta tcgaagacat tgccgacctg ctcggcgatc tcgaacaggc cctgggttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at