MysmA.00906.a

Putative cystathionine gamma-synthase

CENTER ID: MysmA.00906.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00906.a.A1.GE28849 Full length( MysmA.00906.a ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00906.a.A1.PS00609 Full length( MysmA.00906.a ) 1 384
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126775
PATRIC ID: fig|246196.19.peg.5136
RefSeq: YP_889511.1
UniProt: A0R2X3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEHRKWHGL ATKAIHAGYQ PDPATGAVNT PIYASSTFAQ DGVGGLRGGF EYARTGNPTR QSLEAALAAV EEARFGRAFS SGMAATDCAL RAVLRPGDHV VIPDDAYGGT FRLIDKVFTH WGISHTPAAL SDLDSVRSAI TPKTRLIWVE TPTNPLLNVA DIAGIVQIAS STGVKVLVDN TFASPALQQP LLLGADIVLH STTKYIGGHS DVVGGALLTN DEELDAAFAF LQNGAGGVPG PFDAYLTMRG LKTLVLRMRQ HSENAALIAE FLDGHPAVQT VLYPGLPSHP NHDVAARQMT GFGGMVSVRL RGGPQAARDL CSRTEIFILA ESLGGVESLI EYPGAMTHAS TAGSQLEVPD DLVRLSVGIE HSADLLADLE QALG
NT Sequence
ATGAGTGAAC ACCGCAAGTG GCACGGTCTG GCCACGAAAG CCATCCATGC CGGTTACCAG CCTGACCCGG CGACCGGTGC CGTCAACACG CCGATCTACG CCAGCTCGAC GTTCGCCCAG GACGGGGTCG GTGGGCTCCG CGGCGGGTTC GAGTACGCCC GCACCGGCAA CCCGACGCGG CAGTCCCTCG AGGCCGCGCT GGCCGCGGTC GAGGAGGCCC GCTTCGGCCG GGCGTTCAGC TCGGGCATGG CCGCCACCGA CTGCGCGCTG CGTGCGGTGC TGCGTCCGGG CGATCACGTC GTGATCCCCG ACGACGCCTA CGGCGGCACG TTCCGGCTCA TCGACAAGGT CTTCACGCAC TGGGGCATCA GCCACACCCC GGCCGCGCTG AGCGACCTCG ACTCGGTGCG CTCGGCGATC ACGCCCAAGA CCCGCCTGAT CTGGGTCGAG ACCCCGACCA ACCCGCTGCT GAACGTCGCC GACATCGCGG GCATAGTGCA GATCGCGTCG TCGACGGGTG TGAAGGTGTT GGTGGACAAC ACGTTTGCCT CACCGGCTCT GCAGCAGCCG CTGCTGCTGG GCGCCGACAT CGTGCTGCAC TCGACCACCA AGTACATCGG CGGTCACTCC GACGTCGTCG GCGGTGCGCT GCTGACCAAC GACGAGGAAC TCGACGCCGC GTTCGCGTTC CTGCAGAACG GCGCGGGCGG TGTGCCCGGA CCGTTCGACG CGTACCTGAC CATGCGCGGA CTGAAGACCC TGGTGCTGCG GATGCGTCAG CACAGCGAGA ACGCCGCGCT GATCGCGGAA TTCCTCGACG GGCATCCCGC GGTCCAGACC GTGCTGTACC CCGGTCTGCC CAGCCACCCC AACCACGACG TCGCGGCACG GCAGATGACC GGCTTCGGCG GCATGGTGTC GGTGCGGTTG CGTGGTGGCC CACAGGCCGC GCGTGACTTG TGCTCGCGCA CAGAGATCTT CATCCTCGCC GAGTCGCTGG GCGGGGTCGA GTCGCTGATC GAGTACCCGG GCGCGATGAC CCACGCGTCG ACCGCGGGCT CGCAGCTCGA GGTGCCCGAC GACCTGGTGC GGCTGTCGGT CGGCATCGAG CACTCAGCGG ACCTGCTGGC CGACCTGGAG CAGGCCCTGG GC
Details for MysmA.00906.a.A1.GE28849
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEHRKWHG LATKAIHAGY QPDPATGAVN TPIYASSTFA QDGVGGLRGG FEYARTGNPT RQSLEAALAA VEEARFGRAF SSGMAATDCA LRAVLRPGDH VVIPDDAYGG TFRLIDKVFT HWGISHTPAA LSDLDSVRSA ITPKTRLIWV ETPTNPLLNV ADIAGIVQIA SSTGVKVLVD NTFASPALQQ PLLLGADIVL HSTTKYIGGH SDVVGGALLT NDEELDAAFA FLQNGAGGVP GPFDAYLTMR GLKTLVLRMR QHSENAALIA EFLDGHPAVQ TVLYPGLPSH PNHDVAARQM TGFGGMVSVR LRGGPQAARD LCSRTEIFIL AESLGGVESL IEYPGAMTHA STAGSQLEVP DDLVRLSXXX EHSADLLADL EQALG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg aacaccgcaa gtggcacggt ctggccacga aagccatcca tgccggttac cagcctgacc cggcgaccgg tgccgtcaac acgccgatct acgccagctc gacgttcgcc caggacgggg tcggtgggct ccgcggcggg ttcgagtacg cccgcaccgg caacccgacg cggcagtccc tcgaggccgc gctggccgcg gtcgaggagg cccgcttcgg ccgggcgttc agctcgggca tggccgccac cgactgcgcg ctgcgtgcgg tgctgcgtcc gggcgatcac gtcgtgatcc ccgacgacgc ctacggcggc acgttccggc tcatcgacaa ggtcttcacg cactggggca tcagccacac cccggccgcg ctgagcgacc tcgactcggt gcgctcggcg atcacgccca agacccgcct gatctgggtc gagaccccga ccaacccgct gctgaacgtc gccgacatcg cgggcatagt gcagatcgcg tcgtcgacgg gtgtgaaggt gttggtggac aacacgtttg cctcaccggc tctgcagcag ccgctgctgc tgggcgccga catcgtgctg cactcgacca ccaagtacat cggcggtcac tccgacgtcg tcggcggtgc gctgctgacc aacgacgagg aactcgacgc cgcgttcgcg ttcctgcaga acggcgcggg cggtgtgccc ggaccgttcg acgcgtacct gaccatgcgc ggactgaaga ccctggtgct gcggatgcgt cagcacagcg agaacgccgc gctgatcgcg gaattcctcg acgggcatcc cgcggtccag accgtgctgt accccggtct gcccagccac cccaaccacg acgtcgcggc acggcagatg accggcttcg gcggcatggt gtcggtgcgg ttgcgtggtg gcccacaggc cgcgcgtgac ttgtgctcgc gcacagagat cttcatcctc gccgagtcgc tgggcggggt cgagtcgctg atcgagtacc cgggcgcgat gacccacgcg tcgaccgcgg gctcgcagct cgaggtgccc gacgacctgg tgcggctgtc ggnnnncntc gagcactcag cggacctnct ggccgacctg gagcaggccc tgggctaat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEHRKWHG LATKAIHAGY QPDPATGAVN TPIYASSTFA QDGVGGLRGG FEYARTGNPT RQSLEAALAA VEEARFGRAF SSGMAATDCA LRAVLRPGDH VVIPDDAYGG TFRLIDKVFT HWGISHTPAA LSDLDSVRSA ITPKTRLIWV ETPTNPLLNV ADIAGIVQIA SSTGVKVLVD NTFASPALQQ PLLLGADIVL HSTTKYIGGH SDVVGGALLT NDEELDAAFA FLQNGAGGVP GPFDAYLTMR GLKTLVLRMR QHSENAALIA EFLDGHPAVQ TVLYPGLPSH PNHDVAARQM TGFGGMVSVR LRGGPQAARD LCSRTEIFIL AESLGGVESL IEYPGAMTHA STAGSQLEVP DDLVRLSVGI EHSADLLADL EQALG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgaacac cgcaagtggc acggtctggc cacgaaagcc atccatgccg gttaccagcc tgacccggcg accggtgccg tcaacacgcc gatctacgcc agctcgacgt tcgcccagga cggggtcggt gggctccgcg gcgggttcga gtacgcccgc accggcaacc cgacgcggca gtccctcgag gccgcgctgg ccgcggtcga ggaggcccgc ttcggccggg cgttcagctc gggcatggcc gccaccgact gcgcgctgcg tgcggtgctg cgtccgggcg atcacgtcgt gatccccgac gacgcctacg gcggcacgtt ccggctcatc gacaaggtct tcacgcactg gggcatcagc cacaccccgg ccgcgctgag cgacctcgac tcggtgcgct cggcgatcac gcccaagacc cgcctgatct gggtcgagac cccgaccaac ccgctgctga acgtcgccga catcgcgggc atagtgcaga tcgcgtcgtc gacgggtgtg aaggtgttgg tggacaacac gtttgcctca ccggctctgc agcagccgct gctgctgggc gccgacatcg tgctgcactc gaccaccaag tacatcggcg gtcactccga cgtcgtcggc ggtgcgctgc tgaccaacga cgaggaactc gacgccgcgt tcgcgttcct gcagaacggc gcgggcggtg tgcccggacc gttcgacgcg tacctgacca tgcgcggact gaagaccctg gtgctgcgga tgcgtcagca cagcgagaac gccgcgctga tcgcggaatt cctcgacggg catcccgcgg tccagaccgt gctgtacccc ggtctgccca gccaccccaa ccacgacgtc gcggcacggc agatgaccgg cttcggcggc atggtgtcgg tgcggttgcg tggtggccca caggccgcgc gtgacttgtg ctcgcgcaca gagatcttca tcctcgccga gtcgctgggc ggggtcgagt cgctgatcga gtacccgggc gcgatgaccc acgcgtcgac cgcgggctcg cagctcgagg tgcccgacga cctggtgcgg ctgtcggtcg gcatcgagca ctcagcggac ctgctggccg acctggagca ggccctgggc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.00906.a.A1.PS00609
PURIFICATION DATe: 5/21/2010
CONCENTRATION: 60.19mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEHRKWHG LATKAIHAGY QPDPATGAVN TPIYASSTFA QDGVGGLRGG FEYARTGNPT RQSLEAALAA VEEARFGRAF SSGMAATDCA LRAVLRPGDH VVIPDDAYGG TFRLIDKVFT HWGISHTPAA LSDLDSVRSA ITPKTRLIWV ETPTNPLLNV ADIAGIVQIA SSTGVKVLVD NTFASPALQQ PLLLGADIVL HSTTKYIGGH SDVVGGALLT NDEELDAAFA FLQNGAGGVP GPFDAYLTMR GLKTLVLRMR QHSENAALIA EFLDGHPAVQ TVLYPGLPSH PNHDVAARQM TGFGGMVSVR LRGGPQAARD LCSRTEIFIL AESLGGVESL IEYPGAMTHA STAGSQLEVP DDLVRLSXXX EHSADLLADL EQALG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg aacaccgcaa gtggcacggt ctggccacga aagccatcca tgccggttac cagcctgacc cggcgaccgg tgccgtcaac acgccgatct acgccagctc gacgttcgcc caggacgggg tcggtgggct ccgcggcggg ttcgagtacg cccgcaccgg caacccgacg cggcagtccc tcgaggccgc gctggccgcg gtcgaggagg cccgcttcgg ccgggcgttc agctcgggca tggccgccac cgactgcgcg ctgcgtgcgg tgctgcgtcc gggcgatcac gtcgtgatcc ccgacgacgc ctacggcggc acgttccggc tcatcgacaa ggtcttcacg cactggggca tcagccacac cccggccgcg ctgagcgacc tcgactcggt gcgctcggcg atcacgccca agacccgcct gatctgggtc gagaccccga ccaacccgct gctgaacgtc gccgacatcg cgggcatagt gcagatcgcg tcgtcgacgg gtgtgaaggt gttggtggac aacacgtttg cctcaccggc tctgcagcag ccgctgctgc tgggcgccga catcgtgctg cactcgacca ccaagtacat cggcggtcac tccgacgtcg tcggcggtgc gctgctgacc aacgacgagg aactcgacgc cgcgttcgcg ttcctgcaga acggcgcggg cggtgtgccc ggaccgttcg acgcgtacct gaccatgcgc ggactgaaga ccctggtgct gcggatgcgt cagcacagcg agaacgccgc gctgatcgcg gaattcctcg acgggcatcc cgcggtccag accgtgctgt accccggtct gcccagccac cccaaccacg acgtcgcggc acggcagatg accggcttcg gcggcatggt gtcggtgcgg ttgcgtggtg gcccacaggc cgcgcgtgac ttgtgctcgc gcacagagat cttcatcctc gccgagtcgc tgggcggggt cgagtcgctg atcgagtacc cgggcgcgat gacccacgcg tcgaccgcgg gctcgcagct cgaggtgccc gacgacctgg tgcggctgtc ggnnnncntc gagcactcag cggacctnct ggccgacctg gagcaggccc tgggctaat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEHRKWHG LATKAIHAGY QPDPATGAVN TPIYASSTFA QDGVGGLRGG FEYARTGNPT RQSLEAALAA VEEARFGRAF SSGMAATDCA LRAVLRPGDH VVIPDDAYGG TFRLIDKVFT HWGISHTPAA LSDLDSVRSA ITPKTRLIWV ETPTNPLLNV ADIAGIVQIA SSTGVKVLVD NTFASPALQQ PLLLGADIVL HSTTKYIGGH SDVVGGALLT NDEELDAAFA FLQNGAGGVP GPFDAYLTMR GLKTLVLRMR QHSENAALIA EFLDGHPAVQ TVLYPGLPSH PNHDVAARQM TGFGGMVSVR LRGGPQAARD LCSRTEIFIL AESLGGVESL IEYPGAMTHA STAGSQLEVP DDLVRLSVGI EHSADLLADL EQALG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGAACAC CGCAAGTGGC ACGGTCTGGC CACGAAAGCC ATCCATGCCG GTTACCAGCC TGACCCGGCG ACCGGTGCCG TCAACACGCC GATCTACGCC AGCTCGACGT TCGCCCAGGA CGGGGTCGGT GGGCTCCGCG GCGGGTTCGA GTACGCCCGC ACCGGCAACC CGACGCGGCA GTCCCTCGAG GCCGCGCTGG CCGCGGTCGA GGAGGCCCGC TTCGGCCGGG CGTTCAGCTC GGGCATGGCC GCCACCGACT GCGCGCTGCG TGCGGTGCTG CGTCCGGGCG ATCACGTCGT GATCCCCGAC GACGCCTACG GCGGCACGTT CCGGCTCATC GACAAGGTCT TCACGCACTG GGGCATCAGC CACACCCCGG CCGCGCTGAG CGACCTCGAC TCGGTGCGCT CGGCGATCAC GCCCAAGACC CGCCTGATCT GGGTCGAGAC CCCGACCAAC CCGCTGCTGA ACGTCGCCGA CATCGCGGGC ATAGTGCAGA TCGCGTCGTC GACGGGTGTG AAGGTGTTGG TGGACAACAC GTTTGCCTCA CCGGCTCTGC AGCAGCCGCT GCTGCTGGGC GCCGACATCG TGCTGCACTC GACCACCAAG TACATCGGCG GTCACTCCGA CGTCGTCGGC GGTGCGCTGC TGACCAACGA CGAGGAACTC GACGCCGCGT TCGCGTTCCT GCAGAACGGC GCGGGCGGTG TGCCCGGACC GTTCGACGCG TACCTGACCA TGCGCGGACT GAAGACCCTG GTGCTGCGGA TGCGTCAGCA CAGCGAGAAC GCCGCGCTGA TCGCGGAATT CCTCGACGGG CATCCCGCGG TCCAGACCGT GCTGTACCCC GGTCTGCCCA GCCACCCCAA CCACGACGTC GCGGCACGGC AGATGACCGG CTTCGGCGGC ATGGTGTCGG TGCGGTTGCG TGGTGGCCCA CAGGCCGCGC GTGACTTGTG CTCGCGCACA GAGATCTTCA TCCTCGCCGA GTCGCTGGGC GGGGTCGAGT CGCTGATCGA GTACCCGGGC GCGATGACCC ACGCGTCGAC CGCGGGCTCG CAGCTCGAGG TGCCCGACGA CCTGGTGCGG CTGTCGGTCG GCATCGAGCA CTCAGCGGAC CTGCTGGCCG ACCTGGAGCA GGCCCTGGGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA