MysmA.00906.b

Putative cystathionine gamma-synthase

CENTER ID: MysmA.00906.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00906.b.A1.PW28755 Full length( MysmA.00906.b ) 1 369

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2358
RefSeq: YP_886734.1
UniProt: A0QUZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGTYGDSTR SVKAVGVDAV PGQPVAPMPV PASTYHLSPD ATEPLDSYGR SSNPSWRQLE SALAELEGAS WALTYGSGMA AITSTLRALT RPGTTLVVPA DGYYQVRRYG AEYLAQQGVS VIEAHSHEMC DAAEKADVVL AETPANPGLD VVDLHRLAMI CRARKATLVV DNTTATPMGQ QPLSLGADLV VASATKSLSG HSDLIAGYVA GCQPELAERI ERDRLLAGPI LGAFEAWLVL RSLGSAGLRF ERQCQNALAL AVMLRGHPAV RSVRYPGLPE DPAHAVAAEQ MKHFGGLVSF ELADAAAVNA LVERSELLIA STSFGGIHTS VDRRARWGDP VPEGFVRISA GIEDTDDLLA DVERALPAG
NT Sequence
ATGGACGGCA CCTACGGAGA TTCCACTCGC AGCGTCAAAG CCGTTGGGGT GGACGCGGTT CCGGGGCAGC CCGTGGCGCC CATGCCGGTG CCCGCCTCGA CCTATCACCT GTCGCCCGAC GCGACCGAAC CGCTGGACAG CTACGGCCGC AGCTCGAACC CGTCGTGGCG GCAGCTGGAG TCGGCGCTGG CCGAACTCGA AGGTGCCTCG TGGGCGTTGA CCTACGGCTC CGGGATGGCC GCGATCACCT CGACGCTGCG CGCGCTGACC CGGCCGGGCA CCACGCTGGT CGTCCCCGCT GACGGGTACT ACCAGGTGCG GCGATACGGC GCGGAATATC TTGCCCAACA AGGTGTTTCG GTCATCGAAG CGCACTCGCA CGAGATGTGC GATGCCGCGG AGAAGGCCGA CGTGGTACTC GCCGAGACAC CGGCCAATCC GGGACTGGAC GTCGTCGACC TGCACCGTCT GGCGATGATC TGCCGGGCCC GCAAGGCCAC CCTGGTCGTC GACAACACCA CCGCGACACC GATGGGACAG CAACCGCTTT CGCTCGGCGC CGATCTGGTG GTGGCCAGCG CGACGAAATC GTTGTCCGGA CACAGTGACC TGATCGCGGG TTACGTGGCG GGCTGCCAGC CGGAACTGGC GGAGCGCATC GAACGCGACC GGCTGCTGGC CGGTCCCATC CTCGGGGCGT TCGAGGCCTG GCTGGTGCTG CGCAGCCTCG GTAGTGCGGG GCTGCGCTTC GAACGGCAGT GCCAGAACGC GCTCGCCCTG GCGGTCATGC TGCGCGGCCA TCCGGCCGTG CGCTCGGTGC GGTATCCGGG GCTGCCGGAA GATCCGGCAC ACGCCGTGGC CGCCGAGCAG ATGAAACATT TCGGTGGGCT GGTGTCGTTC GAACTGGCCG ACGCCGCTGC GGTCAACGCG CTCGTCGAAC GCAGCGAGTT GCTCATCGCC TCGACGAGCT TCGGCGGCAT CCACACCTCC GTCGACCGCC GCGCGCGCTG GGGTGATCCG GTGCCCGAGG GGTTCGTGCG CATCTCCGCC GGGATCGAGG ACACCGACGA TCTGCTCGCC GACGTCGAAC GAGCCCTGCC CGCCGGG
Details for MysmA.00906.b.A1.PW28755
PURIFICATION DATe: 5/17/2010
CONCENTRATION: 29mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 90
Protocol Notes
notes unavailable
Validated AA Sequence
MDGTYGDSTR SVKAVGVDAV PGQPVAPMPV PASTYHLSPD ATEPLDSYGR SSNPSWRQLE SALAELEGAS WALTYGSGMA AITSTLRALT RPGTTLVVPA DGYYQVRRYG AEYLAQQGVS VIEAHSHEMC DAAEKADVVL AETPANPGLD VVDLHRLAMI CRARKATLVV DNTTATPMGQ QPLSLGADLV VASATKSLSG HSDLIAGYVA GCQPELAXRI ERDRLLXGPI LGAFEAWLVL RSLGSAGLRF XRQCQNALAL AVMLRGHPAV RSVRYPGLPE DPAHAVAXEQ MKHFGGLVSF ELXDAXAVNA LVERSELLIA XXXFGGIHTS VDRRARWGDP VPEGFVRXSX GI
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atggacggca cctacggaga ttccactcgc agcgtcaaag ccgttggggt ggacgcggtt ccggggcagc ccgtggcgcc catgccggtg cccgcctcga cctatcacct gtcgcccgac gcgaccgaac cgctggacag ctacggccgc agctcgaacc cgtcgtggcg gcagctggag tcggcgctgg ccgaactcga aggtgcctcg tgggcgttga cctacggctc cgggatggcc gcgatcacct cgacgctgcg cgcgctgacc cggccgggca ccacgctggt cgtccccgct gacgggtact accaggtgcg gcgatacggc gcggaatatc ttgcccaaca aggtgtttcg gtcatcgaag cgcactcgca cgagatgtgc gatgccgcgg agaaggccga cgtggtactc gccgagacac cggccaatcc gggactggac gtcgtcgacc tgcaccgtct ggcgatgatc tgccgggccc gcaaggccac cctggtcgtc gacaacacca ccgcgacacc gatgggacag caaccgcttt cgctcggcgc cgatctggtg gtggccagcg cgacgaaatc gttgtccgga cacagtgacc tgatcgcggg ttacgtggcg ggctgccagc cggaactggc ggancgcatc gaacgcgacc ggctgctggn cggtcccatc ctcggggcgt tcgaggcctg gctggtgctg cgcagcctcg gtagtgcggg gctgcgcttc naacggcagt gccagaacgc gctcgccctg gcggtcatgc tgcgcggcca tccggccgtg cgctcggtgc ggtatccggg gctgccggaa gatccggcac acgccgtggc nnccgagcag atgaaacatt tcggtgggct ggtgtcgttc gaactgncng acgccgnngc ggtcaacgcg ctcgtcgaac gcagcgagtt gctcatcgcc tngncgagnt tcggcggcat ccacacctcc gtcgaccgcc gcgcgcgctg gggtgatccg gtgcccgagg ggttcgtgcg catntccncc gggatcga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDGTYGDST RSVKAVGVDA VPGQPVAPMP VPASTYHLSP DATEPLDSYG RSSNPSWRQL ESALAELEGA SWALTYGSGM AAITSTLRAL TRPGTTLVVP ADGYYQVRRY GAEYLAQQGV SVIEAHSHEM CDAAEKADVV LAETPANPGL DVVDLHRLAM ICRARKATLV VDNTTATPMG QQPLSLGADL VVASATKSLS GHSDLIAGYV AGCQPELAER IERDRLLAGP ILGAFEAWLV LRSLGSAGLR FERQCQNALA LAVMLRGHPA VRSVRYPGLP EDPAHAVAAE QMKHFGGLVS FELADAAAVN ALVERSELLI ASTSFGGIHT SVDRRARWGD PVPEGFVRIS AGIEDTDDLL ADVERALPAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGGCACC TACGGAGATT CCACTCGCAG CGTCAAAGCC GTTGGGGTGG ACGCGGTTCC GGGGCAGCCC GTGGCGCCCA TGCCGGTGCC CGCCTCGACC TATCACCTGT CGCCCGACGC GACCGAACCG CTGGACAGCT ACGGCCGCAG CTCGAACCCG TCGTGGCGGC AGCTGGAGTC GGCGCTGGCC GAACTCGAAG GTGCCTCGTG GGCGTTGACC TACGGCTCCG GGATGGCCGC GATCACCTCG ACGCTGCGCG CGCTGACCCG GCCGGGCACC ACGCTGGTCG TCCCCGCTGA CGGGTACTAC CAGGTGCGGC GATACGGCGC GGAATATCTT GCCCAACAAG GTGTTTCGGT CATCGAAGCG CACTCGCACG AGATGTGCGA TGCCGCGGAG AAGGCCGACG TGGTACTCGC CGAGACACCG GCCAATCCGG GACTGGACGT CGTCGACCTG CACCGTCTGG CGATGATCTG CCGGGCCCGC AAGGCCACCC TGGTCGTCGA CAACACCACC GCGACACCGA TGGGACAGCA ACCGCTTTCG CTCGGCGCCG ATCTGGTGGT GGCCAGCGCG ACGAAATCGT TGTCCGGACA CAGTGACCTG ATCGCGGGTT ACGTGGCGGG CTGCCAGCCG GAACTGGCGG AGCGCATCGA ACGCGACCGG CTGCTGGCCG GTCCCATCCT CGGGGCGTTC GAGGCCTGGC TGGTGCTGCG CAGCCTCGGT AGTGCGGGGC TGCGCTTCGA ACGGCAGTGC CAGAACGCGC TCGCCCTGGC GGTCATGCTG CGCGGCCATC CGGCCGTGCG CTCGGTGCGG TATCCGGGGC TGCCGGAAGA TCCGGCACAC GCCGTGGCCG CCGAGCAGAT GAAACATTTC GGTGGGCTGG TGTCGTTCGA ACTGGCCGAC GCCGCTGCGG TCAACGCGCT CGTCGAACGC AGCGAGTTGC TCATCGCCTC GACGAGCTTC GGCGGCATCC ACACCTCCGT CGACCGCCGC GCGCGCTGGG GTGATCCGGT GCCCGAGGGG TTCGTGCGCA TCTCCGCCGG GATCGAGGAC ACCGACGATC TGCTCGCCGA CGTCGAACGA GCCCTGCCCG CCGGGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA