MyleA.00906.a

Cystathionine [gamma]-synthase

CENTER ID: MyleA.00906.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00906.a.A1.GE29242 Full length( MyleA.00906.a ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4627
RefSeq: YP_002504180.1
UniProt: A0A0H3MWG0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEDYRGHHG ITGLATKAIH AGYRPDPATG AVNVPIYASS TFAQDGVGEL RGGFEYARTG NPMRAALEAS LATVEEGVFA RAFSSGMAAS DCALRVMLRP GDHVIIPDDV YGGTFRLIDK VFTQWNVDYT PVPLSDLDAV RAAITSRTRL IWVETPTNPL LSIADITSIG ELGKKHSVKV LVDNTFASPA LQQPLMLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDQ AFGFLQNGAG AVPSPFDAYL TMRGLKTLVL RMQRHNENAI TVAEFLAGHP SVSAVLYPGL PSHPGHEVAA RQMRGFGGMV SLRMRAGRLA AQDLCARTKV FTLAESLGGV ESLIEQPSAM THASTTGSQL EVPDDLVRLS VGIEDVGDLL CDLKQALN
NT Sequence
ATGAGCGAAG ATTACCGGGG ACACCACGGC ATTACCGGAC TAGCCACCAA AGCCATCCAT GCTGGCTATC GTCCGGATCC GGCAACAGGG GCAGTGAATG TCCCGATTTA TGCCAGTAGT ACTTTTGCCC AAGATGGCGT CGGTGAGTTG CGTGGCGGAT TCGAATACGC GCGTACCGGC AACCCCATGC GCGCCGCTTT AGAGGCATCC TTGGCCACGG TCGAAGAGGG CGTTTTTGCG CGAGCCTTCA GTTCCGGAAT GGCTGCTAGC GACTGTGCCT TGCGGGTCAT GCTGCGGCCG GGGGACCACG TGATCATCCC GGATGACGTC TACGGCGGCA CCTTCCGGCT GATAGACAAG GTCTTTACTC AATGGAACGT TGACTACACG CCGGTACCGC TGTCTGATTT GGACGCGGTC CGCGCCGCGA TCACATCACG GACCCGGCTG ATATGGGTGG AAACACCGAC CAATCCGCTG CTGTCCATCG CAGATATCAC CAGCATCGGC GAACTAGGCA AAAAGCACTC AGTAAAGGTG TTGGTGGACA ACACCTTTGC TTCACCCGCG CTGCAACAGC CGCTGATGCT GGGGGCAGAC GTCGTGTTGC ACTCGACCAC AAAGTACATC GGCGGCCACT CTGATGTGGT GGGCGGCGCG CTAGTCACCA ACGACGAAGA GCTGGACCAG GCTTTCGGCT TCTTGCAGAA CGGAGCCGGT GCGGTGCCGA GCCCGTTCGA CGCGTACCTA ACGATGCGCG GATTGAAGAC TTTAGTGCTG CGGATGCAGC GGCACAACGA AAATGCCATT ACTGTAGCGG AATTCCTGGC TGGGCATCCG TCGGTGAGCG CCGTGCTGTA TCCGGGCTTG CCCAGCCATC CCGGGCATGA GGTCGCTGCA CGGCAGATGC GCGGCTTCGG CGGCATGGTT TCGTTGCGGA TGCGAGCCGG CCGACTAGCC GCCCAGGATC TGTGTGCCCG CACCAAGGTG TTTACCTTGG CTGAATCCTT GGGTGGAGTG GAGTCGCTGA TTGAGCAGCC CAGTGCCATG ACGCACGCGT CGACAACCGG GTCGCAATTG GAAGTACCCG ACGACCTGGT GCGGCTTTCG GTCGGTATTG AAGACGTCGG CGACCTGCTG TGCGACCTCA AGCAGGCGTT AAAC
Details for MyleA.00906.a.A1.GE29242
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSEDYRGHHG ITGLATKAIH AGYRPDPATG AVNVPIYASS TFAQDGVGEL RGGFEYARTG NPMRAALEAS LATVEEGVFA RAFSSGMAAS DCALRVMLRP GDHVIIPDDV YGGTFRLIDK VFTQWNVDYT PVPLSDLDAV RAAITSRTRL IWVETPTNPL LSIADITSIG ELGKKHSVKV LVDNTFASPA LQQPLMLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDQ AFGFLQNGAG AVPSPFDAYL TMRGLKTLVL RMQRHNENAI TVAEFLAGHP SVSAVLYPGL PSHPGHEVAA RQMRGFGGMV SLRMRAGRLA AQDLCARTKV FTLAESLGGV ESLIEQPSAM THASTTGSQL EVPDDLVRLS VGIEDVGDLL CDLKQALN
Validated NT Sequence
ttattagttt aacgcctgct tgaggtcgca cagcaggtcg ccgacgtctt caataccgac cgaaagccgc accaggtcgt cgggtacttc caattgcgac ccggttgtcg acgcgtgcgt catggcactg ggctgctcaa tcagcgactc cactccaccc aaggattcag ccaaggtaaa caccttggtg cgggcacaca gatcctgggc ggctagtcgg ccggctcgca tccgcaacga aaccatgccg ccgaagccgc gcatctgccg tgcagcgacc tcatgcccgg gatggctggg caagcccgga tacagcacgg cgctcaccga cggatgccca gccaggaatt ccgctacagt aatggcattt tcgttgtgcc gctgcatccg cagcactaaa gtcttcaatc cgcgcatcgt taggtacgcg tcgaacgggc tcggcaccgc accggctccg ttctgcaaga agccgaaagc ctggtccagc tcttcgtcgt tggtgactag cgcgccgccc accacatcag agtggccgcc gatgtacttt gtggtcgagt gcaacacgac gtctgccccc agcatcagcg gctgttgcag cgcgggtgaa gcaaaggtgt tgtccaccaa cacctttact gagtgctttt tgcctagttc gccgatgctg gtgatatctg cgatggacag cagcggattg gtcggtgttt ccacccatat cagccgggtc cgtgatgtga tcgcggcgcg gaccgcgtcc aaatcagaca gcggtaccgg cgtgtagtca acgttccatt gagtaaagac cttgtctatc agccggaagg tgccgccgta gacgtcatcc gggatgatca cgtggtcccc cggccgcagc atgacccgca aggcacagtc gctagcagcc attccggaac tgaaggctcg cgcaaaaacg ccctcttcga ccgtggccaa ggatgcctct aaagcggcgc gcatggggtt gccggtacgc gcgtattcga atccgccacg caactcaccg acgccatctt gggcaaaagt actactggca taaatcggga cattcactgc ccctgttgcc ggatccggac gatagccagc atggatggct ttggtggcta gtccggtaat gccgtggtgt ccccggtaat cttcgctcat cgaaccagga ccctgggtct gagcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEDYRGHH GITGLATKAI HAGYRPDPAT GAVNVPIYAS STFAQDGVGE LRGGFEYART GNPMRAALEA SLATVEEGVF ARAFSSGMAA SDCALRVMLR PGDHVIIPDD VYGGTFRLID KVFTQWNVDY TPVPLSDLDA VRAAITSRTR LIWVETPTNP LLSIADITSI GELGKKHSVK VLVDNTFASP ALQQPLMLGA DVVLHSTTKY IGGHSDVVGG ALVTNDEELD QAFGFLQNGA GAVPSPFDAY LTMRGLKTLV LRMQRHNENA ITVAEFLAGH PSVSAVLYPG LPSHPGHEVA ARQMRGFGGM VSLRMRAGRL AAQDLCARTK VFTLAESLGG VESLIEQPSA MTHASTTGSQ LEVPDDLVRL SVGIEDVGDL LCDLKQALN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaagat taccggggac accacggcat taccggacta gccaccaaag ccatccatgc tggctatcgt ccggatccgg caacaggggc agtgaatgtc ccgatttatg ccagtagtac ttttgcccaa gatggcgtcg gtgagttgcg tggcggattc gaatacgcgc gtaccggcaa ccccatgcgc gccgctttag aggcatcctt ggccacggtc gaagagggcg tttttgcgcg agccttcagt tccggaatgg ctgctagcga ctgtgccttg cgggtcatgc tgcggccggg ggaccacgtg atcatcccgg atgacgtcta cggcggcacc ttccggctga tagacaaggt ctttactcaa tggaacgttg actacacgcc ggtaccgctg tctgatttgg acgcggtccg cgccgcgatc acatcacgga cccggctgat atgggtggaa acaccgacca atccgctgct gtccatcgca gatatcacca gcatcggcga actaggcaaa aagcactcag taaaggtgtt ggtggacaac acctttgctt cacccgcgct gcaacagccg ctgatgctgg gggcagacgt cgtgttgcac tcgaccacaa agtacatcgg cggccactct gatgtggtgg gcggcgcgct agtcaccaac gacgaagagc tggaccaggc tttcggcttc ttgcagaacg gagccggtgc ggtgccgagc ccgttcgacg cgtacctaac gatgcgcgga ttgaagactt tagtgctgcg gatgcagcgg cacaacgaaa atgccattac tgtagcggaa ttcctggctg ggcatccgtc ggtgagcgcc gtgctgtatc cgggcttgcc cagccatccc gggcatgagg tcgctgcacg gcagatgcgc ggcttcggcg gcatggtttc gttgcggatg cgagccggcc gactagccgc ccaggatctg tgtgcccgca ccaaggtgtt taccttggct gaatccttgg gtggagtgga gtcgctgatt gagcagccca gtgccatgac gcacgcgtcg acaaccgggt cgcaattgga agtacccgac gacctggtgc ggctttcggt cggtattgaa gacgtcggcg acctgctgtg cgacctcaag caggcgttaa acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa