MyulA.00906.a
Cystathionine gamma-synthase MetB (Cgs)
| CENTER ID: | MyulA.00906.a |
| ORGANISM: | Mycobacterium ulcerans Agy99 |
| ASSOCIATED DISEASE: | Buruli, Bairnsdale ulcer |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.00906.a.A1.GE29748 | Full length( MyulA.00906.a ) | 1 | 388 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyulA.00906.a.A1.PS00784 | Full length( MyulA.00906.a ) | 1 | 388 |
Structures
3QI6
DEPOSITED: 1/26/2011
DETERMINATION: XRay
CLONE: MyulA.00906.a.A1.GE29748
PROTEIN: MyulA.00906.a.A1.PS00784
3QHX
DEPOSITED: 1/26/2011
DETERMINATION: XRay
CLONE: MyulA.00906.a.A1.GE29748
PROTEIN: MyulA.00906.a.A1.PS00784
Publications by SSGCID
Structure of the cystathionine γ-synthase MetB from Mycobacterium ulcerans.
Abendroth J, Clifton MC, Dieterich SH, Edwards TE, Exley I, Ferrell M, Gillespie AK, Leibly DJ, Myler PJ, Staker BL, Stewart LJ, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1154-8
PMID: 21904066; PMCID: PMC3169418
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126775 |
| PATRIC ID: | fig|362242.7.peg.317 |
| RefSeq: | YP_904423.1 |
| UniProt: | A0PKT3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDDHKAQHR FAGLATRAIH SGYRPDPATG AVNAPIYASS TFAQDGVGGL RGGYEYARTG NPTRTALEAA LAAVEDAAFG RAFSSGMAAA DCALRAMLRP GDHVVIPDDA YGGTFRLIDK VFTGWNVEYT PVALADLDAV RAAIRPTTRL IWVETPTNPL LSIADIAGIA QLGADSSAKV LVDNTFASPA LQQPLSLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDQ SFAFLQNGAG AVPGPFDAYL TMRGLKTLVL RMQRHSENAA AVAEFLAEHP AISTVLYPGL PSHPGHAVAA RQMRGFGGMV SVRMRAGRTA AEQLCAKTNI FILAESLGSV ESLIEHPSAM THASTAGSQL EVPDDLVRLS VGIEDVADLL DDLKQALG
NT Sequence
ATGAAGGACG ATCACAAGGC GCAGCACCGG TTCGCCGGAC TCGCAACACG AGCCATTCAT TCCGGCTATC GCCCGGACCC GGCGACCGGA GCCGTCAACG CTCCGATCTA TGCCAGCAGC ACCTTCGCCC AGGATGGTGT CGGCGGGCTT CGGGGCGGCT ATGAGTATGC GCGAACCGGC AATCCGACTC GCACCGCCTT GGAAGCCGCG CTTGCCGCGG TTGAAGACGC GGCGTTCGGG CGTGCGTTCA GTTCCGGCAT GGCCGCCGCC GACTGCGCGC TGCGGGCAAT GCTGCGACCG GGTGACCATG TCGTCATCCC CGACGACGCC TACGGCGGCA CCTTCCGGTT GATTGACAAG GTCTTCACCG GGTGGAACGT CGAGTACACA CCGGTAGCCC TGGCGGATCT GGATGCGGTG CGTGCGGCGA TAAGGCCAAC CACCCGACTG ATTTGGGTGG AAACACCGAC CAATCCGCTG CTGTCGATCG CCGACATCGC CGGCATCGCC CAGCTGGGTG CGGACAGCTC GGCGAAAGTT CTGGTGGACA ACACCTTCGC ATCTCCGGCG CTGCAGCAGC CGCTATCGCT GGGCGCCGAC GTGGTGCTGC ATTCCACCAC CAAATACATC GGTGGTCACT CCGATGTGGT GGGTGGCGCA CTGGTGACCA ATGACGAAGA GCTGGACCAG TCTTTTGCCT TCTTGCAGAA CGGGGCGGGC GCGGTGCCGG GCCCGTTCGA CGCGTACCTG ACCATGCGCG GCCTGAAAAC CCTGGTCCTG CGCATGCAGC GGCATAGTGA AAATGCCGCT GCCGTGGCGG AATTCCTCGC CGAACATCCC GCGATCAGCA CCGTGTTGTA CCCAGGGCTG CCCAGTCACC CCGGCCATGC CGTGGCGGCG CGGCAGATGC GCGGGTTTGG CGGCATGGTT TCGGTGCGGA TGCGAGCCGG CCGCACCGCC GCCGAACAGC TGTGCGCCAA GACGAACATC TTCATCCTGG CCGAATCGCT AGGCAGTGTG GAGTCGCTGA TCGAGCATCC CAGCGCGATG ACTCACGCGT CGACCGCGGG TTCGCAGCTG GAAGTGCCCG ATGACCTGGT GCGGCTGTCG GTCGGTATCG AAGACGTTGC CGATCTCCTC GACGACCTCA AACAGGCGCT GGGC
Details for MyulA.00906.a.A1.GE29748
| HARVESTED ON: | 6/29/2010 |
| SEQUENCED ON: | 7/7/2010 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MKDDHKAQHR FAGLATRAIH SGYRPDPATG AVNAPIYASS TFAQDGVGGL RGGYEYARTG NPTRTALEAA LAAVEDAAFG RAFSSGMAAA DCALRAMLRP GDHVVIPDDA YGGTFRLIDK VFTGWNVEYT PVALADLDAV RAAIRPTTRL IWVETPTNPL LSIADIAGIA QLGADSSAKV LVDNTFASPA LQQPLSLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDQ SFAFLQNGAG AVPGPFDAYL TMRGLKTLVL RMQRHSENAA AVAEFLAEHP AISTVLYPGL PSHPGHAVAA RQMRGFGGMV SVRMRAGRTA AEQLCAKTNI FILAESLGSV ESLIEHPSAM THASTAGSQL EVPDDLVRLS VGIEDVADLL DDLKQALG
Validated NT Sequence
agcccagcgc ctgtttgagg tcgtcgagga gatcggcaac gtcttcgata ccgaccgaca gccgcaccag gtcatcgggc acttccagct gcgaacccgc ggtcgacgcg tgagtcatcg cgctgggatg ctcgatcagc gactccacac tgcctagcga ttcggccagg atgaagatgt tcgtcttggc gcacagctgt tcggcggcgg tgcggccggc tcgcatccgc accgaaacca tgccgccaaa cccgcgcatc tgccgcgccg ccacggcatg gccggggtga ctgggcagcc ctgggtacaa cacggtgctg atcgcgggat gttcggcgag gaattccgcc acggcagcgg cattttcact atgccgctgc atgcgcagga ccagggtttt caggccgcgc atggtcaggt acgcgtcgaa cgggcccggc accgcgcccg ccccgttctg caagaaggca aaagactggt ccagctcttc gtcattggtc accagtgcgc cacccaccac atcggagtga ccaccgatgt atttggtggt ggaatgcagc accacgtcgg cgcccagcga tagcggctgc tgcagcgccg gagatgcgaa ggtgttgtcc accagaactt tcgccgagct gtccgcaccc agctgggcga tgccggcgat gtcggcgatc gacagcagcg gattggtcgg tgtttccacc caaatcagtc gggtggttgg ccttatcgcc gcacgcaccg catccagatc cgccagggct accggtgtgt actcgacgtt ccacccggtg aagaccttgt caatcaaccg gaaggtgccg ccgtaggcgt cgtcggggat gacgacatgg tcacccggtc gcagcattgc ccgcagcgcg cagtcggcgg cggccatgcc ggaactgaac gcacgcccga acgccgcgtc ttcaaccgcg gcaagcgcgg cttccaaggc ggtgcgagtc ggattgccgg ttcgcgcata ctcatagccg ccccgaagcc cgccgacacc atcctgggcg aaggtgctgc tggcatagat cggagcgttg acggctccgg tcgccgggtc cgggcgatag ccggaatgaa tggctcgtgt tgcgagtccg gcgaaccggt gctgcgcctt gtgatcgtcc ttcatcgaac caggaccctg ggtctgagct tccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKDDHKAQH RFAGLATRAI HSGYRPDPAT GAVNAPIYAS STFAQDGVGG LRGGYEYART GNPTRTALEA ALAAVEDAAF GRAFSSGMAA ADCALRAMLR PGDHVVIPDD AYGGTFRLID KVFTGWNVEY TPVALADLDA VRAAIRPTTR LIWVETPTNP LLSIADIAGI AQLGADSSAK VLVDNTFASP ALQQPLSLGA DVVLHSTTKY IGGHSDVVGG ALVTNDEELD QSFAFLQNGA GAVPGPFDAY LTMRGLKTLV LRMQRHSENA AAVAEFLAEH PAISTVLYPG LPSHPGHAVA ARQMRGFGGM VSVRMRAGRT AAEQLCAKTN IFILAESLGS VESLIEHPSA MTHASTAGSQ LEVPDDLVRL SVGIEDVADL LDDLKQALG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaggacgat cacaaggcgc agcaccggtt cgccggactc gcaacacgag ccattcattc cggctatcgc ccggacccgg cgaccggagc cgtcaacgct ccgatctatg ccagcagcac cttcgcccag gatggtgtcg gcgggcttcg gggcggctat gagtatgcgc gaaccggcaa tccgactcgc accgccttgg aagccgcgct tgccgcggtt gaagacgcgg cgttcgggcg tgcgttcagt tccggcatgg ccgccgccga ctgcgcgctg cgggcaatgc tgcgaccggg tgaccatgtc gtcatccccg acgacgccta cggcggcacc ttccggttga ttgacaaggt cttcaccggg tggaacgtcg agtacacacc ggtagccctg gcggatctgg atgcggtgcg tgcggcgata aggccaacca cccgactgat ttgggtggaa acaccgacca atccgctgct gtcgatcgcc gacatcgccg gcatcgccca gctgggtgcg gacagctcgg cgaaagttct ggtggacaac accttcgcat ctccggcgct gcagcagccg ctatcgctgg gcgccgacgt ggtgctgcat tccaccacca aatacatcgg tggtcactcc gatgtggtgg gtggcgcact ggtgaccaat gacgaagagc tggaccagtc ttttgccttc ttgcagaacg gggcgggcgc ggtgccgggc ccgttcgacg cgtacctgac catgcgcggc ctgaaaaccc tggtcctgcg catgcagcgg catagtgaaa atgccgctgc cgtggcggaa ttcctcgccg aacatcccgc gatcagcacc gtgttgtacc cagggctgcc cagtcacccc ggccatgccg tggcggcgcg gcagatgcgc gggtttggcg gcatggtttc ggtgcggatg cgagccggcc gcaccgccgc cgaacagctg tgcgccaaga cgaacatctt catcctggcc gaatcgctag gcagtgtgga gtcgctgatc gagcatccca gcgcgatgac tcacgcgtcg accgcgggtt cgcagctgga agtgcccgat gacctggtgc ggctgtcggt cggtatcgaa gacgttgccg atctcctcga cgacctcaaa caggcgctgg gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MyulA.00906.a.A1.PS00784
| PURIFICATION DATe: | 9/27/2010 |
| CONCENTRATION: | 74.17mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MKDDHKAQHR FAGLATRAIH SGYRPDPATG AVNAPIYASS TFAQDGVGGL RGGYEYARTG NPTRTALEAA LAAVEDAAFG RAFSSGMAAA DCALRAMLRP GDHVVIPDDA YGGTFRLIDK VFTGWNVEYT PVALADLDAV RAAIRPTTRL IWVETPTNPL LSIADIAGIA QLGADSSAKV LVDNTFASPA LQQPLSLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDQ SFAFLQNGAG AVPGPFDAYL TMRGLKTLVL RMQRHSENAA AVAEFLAEHP AISTVLYPGL PSHPGHAVAA RQMRGFGGMV SVRMRAGRTA AEQLCAKTNI FILAESLGSV ESLIEHPSAM THASTAGSQL EVPDDLVRLS VGIEDVADLL DDLKQALG
Validated NT Sequence
agcccagcgc ctgtttgagg tcgtcgagga gatcggcaac gtcttcgata ccgaccgaca gccgcaccag gtcatcgggc acttccagct gcgaacccgc ggtcgacgcg tgagtcatcg cgctgggatg ctcgatcagc gactccacac tgcctagcga ttcggccagg atgaagatgt tcgtcttggc gcacagctgt tcggcggcgg tgcggccggc tcgcatccgc accgaaacca tgccgccaaa cccgcgcatc tgccgcgccg ccacggcatg gccggggtga ctgggcagcc ctgggtacaa cacggtgctg atcgcgggat gttcggcgag gaattccgcc acggcagcgg cattttcact atgccgctgc atgcgcagga ccagggtttt caggccgcgc atggtcaggt acgcgtcgaa cgggcccggc accgcgcccg ccccgttctg caagaaggca aaagactggt ccagctcttc gtcattggtc accagtgcgc cacccaccac atcggagtga ccaccgatgt atttggtggt ggaatgcagc accacgtcgg cgcccagcga tagcggctgc tgcagcgccg gagatgcgaa ggtgttgtcc accagaactt tcgccgagct gtccgcaccc agctgggcga tgccggcgat gtcggcgatc gacagcagcg gattggtcgg tgtttccacc caaatcagtc gggtggttgg ccttatcgcc gcacgcaccg catccagatc cgccagggct accggtgtgt actcgacgtt ccacccggtg aagaccttgt caatcaaccg gaaggtgccg ccgtaggcgt cgtcggggat gacgacatgg tcacccggtc gcagcattgc ccgcagcgcg cagtcggcgg cggccatgcc ggaactgaac gcacgcccga acgccgcgtc ttcaaccgcg gcaagcgcgg cttccaaggc ggtgcgagtc ggattgccgg ttcgcgcata ctcatagccg ccccgaagcc cgccgacacc atcctgggcg aaggtgctgc tggcatagat cggagcgttg acggctccgg tcgccgggtc cgggcgatag ccggaatgaa tggctcgtgt tgcgagtccg gcgaaccggt gctgcgcctt gtgatcgtcc ttcatcgaac caggaccctg ggtctgagct tccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKDDHKAQH RFAGLATRAI HSGYRPDPAT GAVNAPIYAS STFAQDGVGG LRGGYEYART GNPTRTALEA ALAAVEDAAF GRAFSSGMAA ADCALRAMLR PGDHVVIPDD AYGGTFRLID KVFTGWNVEY TPVALADLDA VRAAIRPTTR LIWVETPTNP LLSIADIAGI AQLGADSSAK VLVDNTFASP ALQQPLSLGA DVVLHSTTKY IGGHSDVVGG ALVTNDEELD QSFAFLQNGA GAVPGPFDAY LTMRGLKTLV LRMQRHSENA AAVAEFLAEH PAISTVLYPG LPSHPGHAVA ARQMRGFGGM VSVRMRAGRT AAEQLCAKTN IFILAESLGS VESLIEHPSA MTHASTAGSQ LEVPDDLVRL SVGIEDVADL LDDLKQALG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGGACGAT CACAAGGCGC AGCACCGGTT CGCCGGACTC GCAACACGAG CCATTCATTC CGGCTATCGC CCGGACCCGG CGACCGGAGC CGTCAACGCT CCGATCTATG CCAGCAGCAC CTTCGCCCAG GATGGTGTCG GCGGGCTTCG GGGCGGCTAT GAGTATGCGC GAACCGGCAA TCCGACTCGC ACCGCCTTGG AAGCCGCGCT TGCCGCGGTT GAAGACGCGG CGTTCGGGCG TGCGTTCAGT TCCGGCATGG CCGCCGCCGA CTGCGCGCTG CGGGCAATGC TGCGACCGGG TGACCATGTC GTCATCCCCG ACGACGCCTA CGGCGGCACC TTCCGGTTGA TTGACAAGGT CTTCACCGGG TGGAACGTCG AGTACACACC GGTAGCCCTG GCGGATCTGG ATGCGGTGCG TGCGGCGATA AGGCCAACCA CCCGACTGAT TTGGGTGGAA ACACCGACCA ATCCGCTGCT GTCGATCGCC GACATCGCCG GCATCGCCCA GCTGGGTGCG GACAGCTCGG CGAAAGTTCT GGTGGACAAC ACCTTCGCAT CTCCGGCGCT GCAGCAGCCG CTATCGCTGG GCGCCGACGT GGTGCTGCAT TCCACCACCA AATACATCGG TGGTCACTCC GATGTGGTGG GTGGCGCACT GGTGACCAAT GACGAAGAGC TGGACCAGTC TTTTGCCTTC TTGCAGAACG GGGCGGGCGC GGTGCCGGGC CCGTTCGACG CGTACCTGAC CATGCGCGGC CTGAAAACCC TGGTCCTGCG CATGCAGCGG CATAGTGAAA ATGCCGCTGC CGTGGCGGAA TTCCTCGCCG AACATCCCGC GATCAGCACC GTGTTGTACC CAGGGCTGCC CAGTCACCCC GGCCATGCCG TGGCGGCGCG GCAGATGCGC GGGTTTGGCG GCATGGTTTC GGTGCGGATG CGAGCCGGCC GCACCGCCGC CGAACAGCTG TGCGCCAAGA CGAACATCTT CATCCTGGCC GAATCGCTAG GCAGTGTGGA GTCGCTGATC GAGCATCCCA GCGCGATGAC TCACGCGTCG ACCGCGGGTT CGCAGCTGGA AGTGCCCGAT GACCTGGTGC GGCTGTCGGT CGGTATCGAA GACGTTGCCG ATCTCCTCGA CGACCTCAAA CAGGCGCTGG GCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA