MytuD.00768.a

phosphoribosylaminoimidazole synthetase

CENTER ID: MytuD.00768.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00768.a.A1.GE25880 Full length( MytuD.00768.a ) 1 364
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00768.a.A1.PS00203a Full length( MytuD.00768.a ) 1 364

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.903
RefSeq: NP_215324.1
TubercuList: Rv0809
MTB Network Portal: Rv0809
UniProt: I6Y4V6

Orthologues for Rv0809

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 90 100 0 0 0
marinum 88 100 1 1 0
ulcerans 88 100 0 0 0
smegmatis 88 96 1 1 0
leprae 87 100 1 0 0
thermoresistibile 84 77 0 0 0
abscessus 80 95 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDLAKGPGK DPGSRGITYA SAGVDIEAGD RAIDLFKPLA SKATRPEVRG GLGGFAGLFT LRGDYREPVL AASSDGVGTK LAIAQAMDKH DTVGLDLVAM VVDDLVVCGA EPLFLLDYIA VGRIVPERLS AIVAGIADGC MRAGCALLGG ETAEHPGLIE PDHYDISATG VGVVEADNVL GPDRVKPGDV IIAMGSSGLH SNGYSLVRKV LLEIDRMNLA GHVEEFGRTL GEELLEPTRI YAKDCLALAA ETRVRTFCHV TGGGLAGNLQ RVIPHGLIAE VDRGTWTPAP VFTMIAQRGR VRRTEMEKTF NMGVGMIAVV APEDTTRALA VLTARHLDCW VLGTVCKGGK QGPRAKLVGQ HPRF
NT Sequence
ATGACGGATC TCGCAAAAGG CCCCGGAAAA GACCCGGGTA GTCGGGGTAT CACCTACGCG TCGGCCGGGG TCGACATCGA AGCCGGTGAC CGCGCCATCG ACCTGTTCAA GCCGCTCGCT TCGAAGGCCA CCAGACCCGA AGTGCGCGGC GGGCTGGGGG GATTCGCCGG ACTGTTCACT CTCCGCGGTG ACTACCGCGA ACCGGTGCTG GCGGCCTCCA GCGACGGCGT CGGCACCAAA CTCGCGATCG CTCAGGCGAT GGATAAGCAC GACACGGTGG GCCTGGACCT GGTGGCGATG GTGGTCGATG ACTTGGTGGT TTGCGGCGCC GAGCCGCTGT TCCTGTTGGA TTACATCGCC GTCGGTCGGA TCGTGCCGGA GCGACTCAGC GCGATCGTCG CCGGTATCGC CGATGGGTGC ATGCGTGCCG GCTGTGCGCT GCTTGGCGGC GAGACCGCAG AACATCCGGG CCTGATCGAG CCCGATCACT ACGATATCTC TGCCACCGGC GTCGGCGTCG TCGAGGCGGA CAATGTGCTG GGTCCCGACC GGGTCAAACC CGGCGACGTC ATCATCGCGA TGGGCTCGTC GGGTCTGCAT TCCAATGGGT ACTCGCTGGT CCGCAAGGTG TTGCTGGAGA TCGACCGGAT GAATCTGGCC GGTCATGTGG AGGAGTTCGG TCGCACCTTG GGCGAAGAGT TATTGGAGCC GACTCGCATC TACGCCAAAG ACTGTTTGGC CTTGGCCGCC GAAACCCGTG TCCGGACGTT TTGCCACGTC ACCGGCGGCG GGCTCGCCGG CAACCTGCAA CGGGTCATCC CGCATGGCCT CATCGCCGAG GTCGACCGCG GCACCTGGAC ACCCGCGCCG GTATTCACCA TGATTGCCCA GCGCGGCCGG GTCAGGCGCA CAGAGATGGA GAAGACGTTC AACATGGGTG TCGGCATGAT CGCCGTCGTT GCCCCCGAAG ACACGACGCG CGCCCTGGCC GTCCTGACCG CGCGGCACCT GGACTGCTGG GTATTGGGAA CCGTCTGCAA AGGCGGAAAA CAAGGCCCGC GGGCAAAACT GGTTGGGCAG CACCCGAGAT TC
Details for MytuD.00768.a.A1.GE25880
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLAKGPG KDPGSRGITY ASAGVDIEAG DRAIDLFKPL ASKATRPEVR GGLGGFAGLF TLRGDYREPV LAASSDGVGT KLAIAQAMDK HDTVGLDLVA MVVDDLVVCG AEPLFLLDYI AVGRIVPERL SAIVAGIADG CMRAGCALLG GETAEHPGLI EPDHYDISAT GVGVVEADNV LGPDRVKPGD VIIAMGSSGL HSNGYSLVRK VLLEIDRMNL AGHVEEFGRT LGEELLEPTR IYAKDCLALA AETRVRTFCH VTGGGLAGNL QRVIPHGLIA EVDRGTWTPA PVFTMIAQRG RVRRTEMEKT FNMGVGMIAV VAPEDTTRAL AVLTARHLDC WVLGTVCKGG KQGPRAKLVG QHPRF
Validated NT Sequence
ttattagaat ctcgggtgct gcccaaccag ttttgcccgc gggccttgtt ttccgccttt gcagacggtt cccaataccc agcagtccag gtgccgcgcg gtcaggacgg ccagggcgcg cgtcgtgtct tcgggggcaa cgacggcgat catgccgaca cccatgttga acgtcttctc catctctgtg cgcctgaccc ggccgcgctg ggcaatcatg gtgaataccg gcgcgggtgt ccaggtgccg cggtcgacct cggcgatgag gccatgcggg atgacccgtt gcaggttgcc ggcgagcccg ccgccggtga cgtggcaaaa cgtccggaca cgggtttcgg cggccaaggc caaacagtct ttggcgtaga tgcgagtcgg ctccaataac tcttcgccca aggtgcgacc gaactcctcc acatgaccgg ccagattcat ccggtcgatc tccagcaaca ccttgcggac cagcgagtac ccattggaat gcagacccga cgagcccatc gcgatgatga cgtcgccggg tttgacccgg tcgggaccca gcacattgtc cgcctcgacg acgccgacgc cggtggcaga gatatcgtag tgatcgggct cgatcaggcc cggatgttct gcggtctcgc cgccaagcag cgcacagccg gcacgcatgc acccatcggc gataccggcg acgatcgcgc tgagtcgctc cggcacgatc cgaccgacgg cgatgtaatc caacaggaac agcggctcgg cgccgcaaac caccaagtca tcgaccacca tcgccaccag gtccaggccc accgtgtcgt gcttatccat cgcctgagcg atcgcgagtt tggtgccgac gccgtcgctg gaggccgcca gcaccggttc gcggtagtca ccgcggagag tgaacagtcc ggcgaatccc cccagcccgc cgcgcacttc gggtctggtg gccttcgaag cgagcggctt gaacaggtcg atggcgcggt caccggcttc gatgtcgacc ccggccgacg cgtaggtgat accccgacta cccgggtctt ttccggggcc ttttgcgaga tccgtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLAKGPG KDPGSRGITY ASAGVDIEAG DRAIDLFKPL ASKATRPEVR GGLGGFAGLF TLRGDYREPV LAASSDGVGT KLAIAQAMDK HDTVGLDLVA MVVDDLVVCG AEPLFLLDYI AVGRIVPERL SAIVAGIADG CMRAGCALLG GETAEHPGLI EPDHYDISAT GVGVVEADNV LGPDRVKPGD VIIAMGSSGL HSNGYSLVRK VLLEIDRMNL AGHVEEFGRT LGEELLEPTR IYAKDCLALA AETRVRTFCH VTGGGLAGNL QRVIPHGLIA EVDRGTWTPA PVFTMIAQRG RVRRTEMEKT FNMGVGMIAV VAPEDTTRAL AVLTARHLDC WVLGTVCKGG KQGPRAKLVG QHPRF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggatctc gcaaaaggcc ccggaaaaga cccgggtagt cggggtatca cctacgcgtc ggccggggtc gacatcgaag ccggtgaccg cgccatcgac ctgttcaagc cgctcgcttc gaaggccacc agacccgaag tgcgcggcgg gctgggggga ttcgccggac tgttcactct ccgcggtgac taccgcgaac cggtgctggc ggcctccagc gacggcgtcg gcaccaaact cgcgatcgct caggcgatgg ataagcacga cacggtgggc ctggacctgg tggcgatggt ggtcgatgac ttggtggttt gcggcgccga gccgctgttc ctgttggatt acatcgccgt cggtcggatc gtgccggagc gactcagcgc gatcgtcgcc ggtatcgccg atgggtgcat gcgtgccggc tgtgcgctgc ttggcggcga gaccgcagaa catccgggcc tgatcgagcc cgatcactac gatatctctg ccaccggcgt cggcgtcgtc gaggcggaca atgtgctggg tcccgaccgg gtcaaacccg gcgacgtcat catcgcgatg ggctcgtcgg gtctgcattc caatgggtac tcgctggtcc gcaaggtgtt gctggagatc gaccggatga atctggccgg tcatgtggag gagttcggtc gcaccttggg cgaagagtta ttggagccga ctcgcatcta cgccaaagac tgtttggcct tggccgccga aacccgtgtc cggacgtttt gccacgtcac cggcggcggg ctcgccggca acctgcaacg ggtcatcccg catggcctca tcgccgaggt cgaccgcggc acctggacac ccgcgccggt attcaccatg attgcccagc gcggccgggt caggcgcaca gagatggaga agacgttcaa catgggtgtc ggcatgatcg ccgtcgttgc ccccgaagac acgacgcgcg ccctggccgt cctgaccgcg cggcacctgg actgctgggt attgggaacc gtctgcaaag gcggaaaaca aggcccgcgg gcaaaactgg ttgggcagca cccgagattc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MytuD.00768.a.A1.PS00203a
PURIFICATION DATe: 4/9/2009
CONCENTRATION: 40.71mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLAKGPG KDPGSRGITY ASAGVDIEAG DRAIDLFKPL ASKATRPEVR GGLGGFAGLF TLRGDYREPV LAASSDGVGT KLAIAQAMDK HDTVGLDLVA MVVDDLVVCG AEPLFLLDYI AVGRIVPERL SAIVAGIADG CMRAGCALLG GETAEHPGLI EPDHYDISAT GVGVVEADNV LGPDRVKPGD VIIAMGSSGL HSNGYSLVRK VLLEIDRMNL AGHVEEFGRT LGEELLEPTR IYAKDCLALA AETRVRTFCH VTGGGLAGNL QRVIPHGLIA EVDRGTWTPA PVFTMIAQRG RVRRTEMEKT FNMGVGMIAV VAPEDTTRAL AVLTARHLDC WVLGTVCKGG KQGPRAKLVG QHPRF
Validated NT Sequence
ttattagaat ctcgggtgct gcccaaccag ttttgcccgc gggccttgtt ttccgccttt gcagacggtt cccaataccc agcagtccag gtgccgcgcg gtcaggacgg ccagggcgcg cgtcgtgtct tcgggggcaa cgacggcgat catgccgaca cccatgttga acgtcttctc catctctgtg cgcctgaccc ggccgcgctg ggcaatcatg gtgaataccg gcgcgggtgt ccaggtgccg cggtcgacct cggcgatgag gccatgcggg atgacccgtt gcaggttgcc ggcgagcccg ccgccggtga cgtggcaaaa cgtccggaca cgggtttcgg cggccaaggc caaacagtct ttggcgtaga tgcgagtcgg ctccaataac tcttcgccca aggtgcgacc gaactcctcc acatgaccgg ccagattcat ccggtcgatc tccagcaaca ccttgcggac cagcgagtac ccattggaat gcagacccga cgagcccatc gcgatgatga cgtcgccggg tttgacccgg tcgggaccca gcacattgtc cgcctcgacg acgccgacgc cggtggcaga gatatcgtag tgatcgggct cgatcaggcc cggatgttct gcggtctcgc cgccaagcag cgcacagccg gcacgcatgc acccatcggc gataccggcg acgatcgcgc tgagtcgctc cggcacgatc cgaccgacgg cgatgtaatc caacaggaac agcggctcgg cgccgcaaac caccaagtca tcgaccacca tcgccaccag gtccaggccc accgtgtcgt gcttatccat cgcctgagcg atcgcgagtt tggtgccgac gccgtcgctg gaggccgcca gcaccggttc gcggtagtca ccgcggagag tgaacagtcc ggcgaatccc cccagcccgc cgcgcacttc gggtctggtg gccttcgaag cgagcggctt gaacaggtcg atggcgcggt caccggcttc gatgtcgacc ccggccgacg cgtaggtgat accccgacta cccgggtctt ttccggggcc ttttgcgaga tccgtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLAKGPG KDPGSRGITY ASAGVDIEAG DRAIDLFKPL ASKATRPEVR GGLGGFAGLF TLRGDYREPV LAASSDGVGT KLAIAQAMDK HDTVGLDLVA MVVDDLVVCG AEPLFLLDYI AVGRIVPERL SAIVAGIADG CMRAGCALLG GETAEHPGLI EPDHYDISAT GVGVVEADNV LGPDRVKPGD VIIAMGSSGL HSNGYSLVRK VLLEIDRMNL AGHVEEFGRT LGEELLEPTR IYAKDCLALA AETRVRTFCH VTGGGLAGNL QRVIPHGLIA EVDRGTWTPA PVFTMIAQRG RVRRTEMEKT FNMGVGMIAV VAPEDTTRAL AVLTARHLDC WVLGTVCKGG KQGPRAKLVG QHPRF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGATCTC GCAAAAGGCC CCGGAAAAGA CCCGGGTAGT CGGGGTATCA CCTACGCGTC GGCCGGGGTC GACATCGAAG CCGGTGACCG CGCCATCGAC CTGTTCAAGC CGCTCGCTTC GAAGGCCACC AGACCCGAAG TGCGCGGCGG GCTGGGGGGA TTCGCCGGAC TGTTCACTCT CCGCGGTGAC TACCGCGAAC CGGTGCTGGC GGCCTCCAGC GACGGCGTCG GCACCAAACT CGCGATCGCT CAGGCGATGG ATAAGCACGA CACGGTGGGC CTGGACCTGG TGGCGATGGT GGTCGATGAC TTGGTGGTTT GCGGCGCCGA GCCGCTGTTC CTGTTGGATT ACATCGCCGT CGGTCGGATC GTGCCGGAGC GACTCAGCGC GATCGTCGCC GGTATCGCCG ATGGGTGCAT GCGTGCCGGC TGTGCGCTGC TTGGCGGCGA GACCGCAGAA CATCCGGGCC TGATCGAGCC CGATCACTAC GATATCTCTG CCACCGGCGT CGGCGTCGTC GAGGCGGACA ATGTGCTGGG TCCCGACCGG GTCAAACCCG GCGACGTCAT CATCGCGATG GGCTCGTCGG GTCTGCATTC CAATGGGTAC TCGCTGGTCC GCAAGGTGTT GCTGGAGATC GACCGGATGA ATCTGGCCGG TCATGTGGAG GAGTTCGGTC GCACCTTGGG CGAAGAGTTA TTGGAGCCGA CTCGCATCTA CGCCAAAGAC TGTTTGGCCT TGGCCGCCGA AACCCGTGTC CGGACGTTTT GCCACGTCAC CGGCGGCGGG CTCGCCGGCA ACCTGCAACG GGTCATCCCG CATGGCCTCA TCGCCGAGGT CGACCGCGGC ACCTGGACAC CCGCGCCGGT ATTCACCATG ATTGCCCAGC GCGGCCGGGT CAGGCGCACA GAGATGGAGA AGACGTTCAA CATGGGTGTC GGCATGATCG CCGTCGTTGC CCCCGAAGAC ACGACGCGCG CCCTGGCCGT CCTGACCGCG CGGCACCTGG ACTGCTGGGT ATTGGGAACC GTCTGCAAAG GCGGAAAACA AGGCCCGCGG GCAAAACTGG TTGGGCAGCA CCCGAGATTC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA