MyleA.00768.a

Phosphoribosylformylglycinamidine cyclo-ligase

CENTER ID: MyleA.00768.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00768.a.A1.GE34935 Full length( MyleA.00768.a ) 1 364
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4196
RefSeq: YP_002504076.1
UniProt: A0A0H3N0M0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMDPGKSPGG QLGNHGITYA STGVDIEAGD RSVELFKPMA VKAIRTEVLG GLGGFAGLFA LRSGYREPVL AASTDGVGTK LAVAQAMDKH DTVGLDLVAM VVDDLVVCGA EPLFLQDYIA VGRTVPERLS AIVDGIAEGC MLAGCALLGG ETAEHPGLME VDHYDIAATG VGVVEADDTL GPDRVKPGDV IIALRSSGLH SNGYSLARSV LLEIDRMNLA GYVEEFGRTL GEELLEPTRI YAKDCLALAA ETHVRTFCHV TGGGLAGNLQ RVIPHGLVAE VDRGTWTPAP VFAMIAQRGR VAREEMEKTF NMGVGMIAVV APEDTDRALA ILTARHLDCW VLGTVCKGSK VGPRAKLVGQ HPRF
NT Sequence
ATGATGGATC CCGGAAAAAG CCCCGGAGGC CAGCTGGGCA ATCATGGCAT CACCTATGCG TCGACAGGGG TGGACATTGA AGCCGGTGAC CGCTCGGTCG AGTTGTTCAA GCCGATGGCA GTTAAGGCCA TCAGAACCGA GGTGCTTGGA GGTCTAGGAG GATTCGCTGG GTTGTTCGCT CTACGCAGCG GCTACCGCGA ACCGGTGTTA GCGGCTTCCA CCGACGGTGT TGGTACCAAG CTGGCAGTCG CTCAGGCGAT GGATAAGCAC GACACCGTTG GCTTGGACCT GGTGGCGATG GTGGTCGACG ACCTGGTGGT CTGTGGCGCT GAGCCACTGT TTTTGCAGGA CTACATTGCT GTCGGCCGAA CGGTGCCGGA ACGACTGAGC GCGATCGTCG ACGGTATTGC CGAGGGGTGC ATGCTCGCGG GCTGTGCGTT ACTGGGTGGT GAGACCGCAG AACACCCTGG TCTGATGGAA GTCGACCACT ACGACATCGC GGCGACCGGC GTCGGTGTTG TGGAAGCCGA CGATACCCTG GGGCCAGATC GTGTCAAACC CGGTGACGTA ATCATCGCGC TGAGGTCTTC GGGTCTCCAT TCCAACGGAT ACTCGCTGGC TCGCTCGGTC CTACTCGAGA TCGATCGGAT GAACCTGGCC GGTTATGTGG AGGAGTTCGG TCGCACCCTG GGCGAAGAGC TATTGGAGCC CACCCGCATC TACGCCAAGG ACTGTCTGGC ATTGGCGGCC GAAACTCACG TCCGTACCTT CTGTCACGTT ACCGGCGGCG GGCTGGCCGG CAATCTTCAA CGCGTTATCC CACACGGATT GGTCGCCGAA GTTGACCGCG GCACGTGGAC ACCTGCACCG GTGTTCGCCA TGATAGCCCA GCGTGGCAGG GTCGCGCGTG AAGAGATGGA AAAGACGTTC AACATGGGCG TCGGCATGAT CGCCGTGGTC GCGCCTGAAG ATACTGACCG TGCTTTGGCG ATATTGACCG CACGTCACTT GGACTGCTGG GTGCTGGGCA CTGTCTGCAA AGGCAGCAAA GTAGGCCCAC GGGCAAAACT GGTCGGACAA CACCCGAGAT TC
Details for MyleA.00768.a.A1.GE34935
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MMDPGKSPGG QLGNHGITYA STGVDIEAGD RSVELFKPMA VKAIRTEVLG GLGGFAGLFA LRSGYREPVL AASTDGVGTK LAVAQAMDKH DTVGLDLVAM VVDDLVVCGA EPLFLQDYIA VGRTVPERLS AIVDGIAEGC MLAGCALLGG ETAEHPGLME VDHYDIAATG VGVVEADDTL GPDRVKPGDV IIALRSSGLH SNGYSLARSV LLEIDRMNLA GYVEEFGRTL GEELLEPTRI YAKDCLALAA ETHVRTFCHV TGGGLAGNLQ RVIPHGLVAE VDRGTWTPAP VFAMIAQRGR VAREEMEKTF NMGVGMIAVV APEDTDRALA ILTARHLDCW VLGTVCKGSK VGPRAKLVGQ HPR
Validated NT Sequence
gaatctcggg tgttgtccga ccagttttgc ccgtgggcct actttgctgc ctttgcagac agtgcccagc acccagcagt ccaagtgacg tgcggtcaat atcgccaaag cacggtcagt atcttcaggc gcgaccacgg cgatcatgcc gacgcccatg ttgaacgtct tttccatctc ttcacgcgcg accctgccac gctgggctat catggcgaac accggtgcag gtgtccacgt gccgcggtca acttcggcga ccaatccgtg tgggataacg cgttgaagat tgccggccag cccgccgccg gtaacgtgac agaaggtacg gacgtgagtt tcggccgcca atgccagaca gtccttggcg tagatgcggg tgggctccaa tagctcttcg cccagggtgc gaccgaactc ctccacataa ccggccaggt tcatccgatc gatctcgagt aggaccgagc gagccagcga gtatccgttg gaatggagac ccgaagacct cagcgcgatg attacgtcac cgggtttgac acgatctggc cccagggtat cgtcggcttc cacaacaccg acgccggtcg ccgcgatgtc gtagtggtcg acttccatca gaccagggtg ttctgcggtc tcaccaccca gtaacgcaca gcccgcgagc atgcacccct cggcaatacc gtcgacgatc gcgctcagtc gttccggcac cgttcggccg acagcaatgt agtcctgcaa aaacagtggc tcagcgccac agaccaccag gtcgtcgacc accatcgcca ccaggtccaa gccaacggtg tcgtgcttat ccatcgcctg agcgactgcc agcttggtac caacaccgtc ggtggaagcc gctaacaccg gttcgcggta gccgctgcgt agagcgaaca acccagcgaa tcctcctaga cctccaagca cctcggttct gatggcctta actgccatcg gcttgaacaa ctcgaccgag cggtcaccgg cttcaatgtc cacccctgtc gacgcatagg tgatgccatg attgcccagc tggcctccgg ggctttttcc gggatccatc atcgaaccag gaccctgggt ctgagcttcc aggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMDPGKSPG GQLGNHGITY ASTGVDIEAG DRSVELFKPM AVKAIRTEVL GGLGGFAGLF ALRSGYREPV LAASTDGVGT KLAVAQAMDK HDTVGLDLVA MVVDDLVVCG AEPLFLQDYI AVGRTVPERL SAIVDGIAEG CMLAGCALLG GETAEHPGLM EVDHYDIAAT GVGVVEADDT LGPDRVKPGD VIIALRSSGL HSNGYSLARS VLLEIDRMNL AGYVEEFGRT LGEELLEPTR IYAKDCLALA AETHVRTFCH VTGGGLAGNL QRVIPHGLVA EVDRGTWTPA PVFAMIAQRG RVAREEMEKT FNMGVGMIAV VAPEDTDRAL AILTARHLDC WVLGTVCKGS KVGPRAKLVG QHPRF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatggatccc ggaaaaagcc ccggaggcca gctgggcaat catggcatca cctatgcgtc gacaggggtg gacattgaag ccggtgaccg ctcggtcgag ttgttcaagc cgatggcagt taaggccatc agaaccgagg tgcttggagg tctaggagga ttcgctgggt tgttcgctct acgcagcggc taccgcgaac cggtgttagc ggcttccacc gacggtgttg gtaccaagct ggcagtcgct caggcgatgg ataagcacga caccgttggc ttggacctgg tggcgatggt ggtcgacgac ctggtggtct gtggcgctga gccactgttt ttgcaggact acattgctgt cggccgaacg gtgccggaac gactgagcgc gatcgtcgac ggtattgccg aggggtgcat gctcgcgggc tgtgcgttac tgggtggtga gaccgcagaa caccctggtc tgatggaagt cgaccactac gacatcgcgg cgaccggcgt cggtgttgtg gaagccgacg ataccctggg gccagatcgt gtcaaacccg gtgacgtaat catcgcgctg aggtcttcgg gtctccattc caacggatac tcgctggctc gctcggtcct actcgagatc gatcggatga acctggccgg ttatgtggag gagttcggtc gcaccctggg cgaagagcta ttggagccca cccgcatcta cgccaaggac tgtctggcat tggcggccga aactcacgtc cgtaccttct gtcacgttac cggcggcggg ctggccggca atcttcaacg cgttatccca cacggattgg tcgccgaagt tgaccgcggc acgtggacac ctgcaccggt gttcgccatg atagcccagc gtggcagggt cgcgcgtgaa gagatggaaa agacgttcaa catgggcgtc ggcatgatcg ccgtggtcgc gcctgaagat actgaccgtg ctttggcgat attgaccgca cgtcacttgg actgctgggt gctgggcact gtctgcaaag gcagcaaagt aggcccacgg gcaaaactgg tcggacaaca cccgagattc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa