MymaA.00768.a

Phosphoribosylformylglycinamidine cyclo-ligase

CENTER ID: MymaA.00768.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00768.a.A1.GE30069 Full length( MymaA.00768.a ) 1 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00768.a.A1.PS00855 Full length( MymaA.00768.a ) 1 367

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5232
RefSeq: YP_001853139.1
UniProt: B2HH29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDPEKSPES SGRGPASQGV TYAAAGVDIE AGDRAVELFK PLATRATRPE VRGGLGGFAG LFALRNDYRE PVLAASTDGV GTKLAVAQAM DKHDTVGLDL VAMVVDDLVV CGAEPLFLQD YIAVGRTVPE RVSAIVSGIA EGCVRAGCAL LGGETAEHPG LMAPDHYDIS ATGVGVVEAD DVLGPERVKP GDVIIAMGSS GLHSNGYSLA RNVLLEIDRM NLAGYVEEFG RTLGEELLEP TRIYAKDCLA LTAETHVRTF CHVTGGGLAG NLERVIPHGL VAEIDRGTWT PAPVFAMIAQ RGRVVREEME KTFNMGVGMI AVVAPEDTDR ALAILTARHL DCWVLGTVCK GGKEGPRATL VGQHPRF
NT Sequence
ATGACGGATC CCGAAAAAAG TCCAGAAAGC TCCGGAAGAG GCCCAGCCAG CCAAGGCGTC ACGTACGCGG CGGCAGGTGT GGACATCGAA GCCGGCGATC GAGCCGTGGA GTTGTTCAAG CCGTTGGCAA CCCGGGCCAC CAGACCCGAG GTGCGCGGCG GGCTCGGCGG ATTTGCCGGA TTGTTCGCCC TGCGCAACGA CTACCGCGAA CCGGTGCTGG CCGCCTCCAC CGACGGGGTC GGCACCAAGC TGGCGGTCGC GCAGGCGATG GACAAGCACG ACACTGTCGG TCTTGACCTG GTCGCGATGG TGGTCGACGA CCTGGTGGTC TGTGGCGCGG AGCCACTGTT CCTGCAGGAC TACATCGCGG TCGGCCGGAC CGTGCCGGAA CGGGTCAGCG CGATCGTCAG CGGCATCGCG GAGGGGTGTG TGCGCGCTGG CTGCGCCCTG CTGGGGGGTG AGACCGCTGA ACATCCCGGG CTGATGGCAC CCGACCATTA CGACATCTCG GCCACCGGGG TGGGTGTGGT CGAAGCCGAC GACGTGCTGG GGCCGGAACG CGTCAAACCC GGCGACGTCA TCATCGCGAT GGGCTCGTCG GGGCTGCATT CCAACGGTTA CTCGCTGGCC CGCAACGTTC TGCTGGAGAT CGACCGGATG AATCTGGCCG GTTATGTGGA GGAGTTCGGC CGCACGTTGG GTGAAGAGCT ATTGGAGCCC ACCCGGATCT ATGCCAAGGA CTGTCTGGCG CTCACCGCTG AGACGCATGT CCGAACGTTC TGTCACGTCA CGGGTGGCGG GTTGGCCGGC AACCTGGAGC GGGTAATCCC GCATGGGCTG GTCGCCGAGA TCGACCGCGG CACCTGGACA CCCGCGCCGG TGTTCGCGAT GATTGCCCAG CGCGGCCGGG TGGTGCGCGA GGAGATGGAA AAGACCTTCA ACATGGGCGT CGGCATGATC GCCGTCGTAG CCCCTGAAGA CACCGACCGG GCGCTGGCCA TACTGACCGC GCGCCACCTG GACTGCTGGG TGCTGGGCAC CGTCTGCAAG GGCGGCAAAG AGGGCCCACG TGCCACGCTG GTGGGCCAGC ACCCGCGATT C
Details for MymaA.00768.a.A1.GE30069
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Validated AA Sequence
MTDPEKSPES SGRGPASQGV TYAAAGVDIE AGDRAVELFK PLATRATRPE VRGGLGGFAG LFALRNDYRE PVLAASTDGV GTKLAVAQAM DKHDTVGLDL VAMVVDDLVV CGAEPLFLQD YIAVGRTVPE RVSAIVSGIA EGCVRAGCAL LGGETAEHPG LMAPDHYDIS ATGVGVVEAD DVLGPERVKP GDVIIAMGSS GLHSNGYSLA RNVLLEIDRM NLAGYVEEFG RTLGEELLEP TRIYAKDCLA LTAETHVRTF CHVTGGGLAG NLERVIPHGL VAEIDRGTWT PAPVFAMIAQ RGRVVR
Validated NT Sequence
tcgcgcacca cccggccgcg ctgggcaatc atcgcgaaca ccggcgcggg tgtccaggtg ccgcggtcga tctcggcgac cagcccatgc gggattaccc gctccaggtt gccggccaac ccgccacccg tgacgtgaca gaacgttcgg acatgcgtct cagcggtgag cgccagacag tccttggcat agatccgggt gggctccaat agctcttcac ccaacgtgcg gccgaactcc tccacataac cggccagatt catccggtcg atctccagca gaacgttgcg ggccagcgag taaccgttgg aatgcagccc cgacgagccc atcgcgatga tgacgtcgcc gggtttgacg cgttccggcc ccagcacgtc gtcggcttcg accacaccca ccccggtggc cgagatgtcg taatggtcgg gtgccatcag cccgggatgt tcagcggtct caccccccag cagggcgcag ccagcgcgca cacacccctc cgcgatgccg ctgacgatcg cgctgacccg ttccggcacg gtccggccga ccgcgatgta gtcctgcagg aacagtggct ccgcgccaca gaccaccagg tcgtcgacca ccatcgcgac caggtcaaga ccgacagtgt cgtgcttgtc catcgcctgc gcgaccgcca gcttggtgcc gaccccgtcg gtggaggcgg ccagcaccgg ttcgcggtag tcgttgcgca gggcgaacaa tccggcaaat ccgccgagcc cgccgcgcac ctcgggtctg gtggcccggg ttgccaacgg cttgaacaac tccacggctc gatcgccggc ttcgatgtcc acacctgccg ccgcgtacgt gacgccttgg ctggctgggc ctcttccgga gctttctgga cttttttcgg gatccgtcat cgaaccagga ccctgggtct gagcttccag ggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDPEKSPE SSGRGPASQG VTYAAAGVDI EAGDRAVELF KPLATRATRP EVRGGLGGFA GLFALRNDYR EPVLAASTDG VGTKLAVAQA MDKHDTVGLD LVAMVVDDLV VCGAEPLFLQ DYIAVGRTVP ERVSAIVSGI AEGCVRAGCA LLGGETAEHP GLMAPDHYDI SATGVGVVEA DDVLGPERVK PGDVIIAMGS SGLHSNGYSL ARNVLLEIDR MNLAGYVEEF GRTLGEELLE PTRIYAKDCL ALTAETHVRT FCHVTGGGLA GNLERVIPHG LVAEIDRGTW TPAPVFAMIA QRGRVVREEM EKTFNMGVGM IAVVAPEDTD RALAILTARH LDCWVLGTVC KGGKEGPRAT LVGQHPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggatccc gaaaaaagtc cagaaagctc cggaagaggc ccagccagcc aaggcgtcac gtacgcggcg gcaggtgtgg acatcgaagc cggcgatcga gccgtggagt tgttcaagcc gttggcaacc cgggccacca gacccgaggt gcgcggcggg ctcggcggat ttgccggatt gttcgccctg cgcaacgact accgcgaacc ggtgctggcc gcctccaccg acggggtcgg caccaagctg gcggtcgcgc aggcgatgga caagcacgac actgtcggtc ttgacctggt cgcgatggtg gtcgacgacc tggtggtctg tggcgcggag ccactgttcc tgcaggacta catcgcggtc ggccggaccg tgccggaacg ggtcagcgcg atcgtcagcg gcatcgcgga ggggtgtgtg cgcgctggct gcgccctgct ggggggtgag accgctgaac atcccgggct gatggcaccc gaccattacg acatctcggc caccggggtg ggtgtggtcg aagccgacga cgtgctgggg ccggaacgcg tcaaacccgg cgacgtcatc atcgcgatgg gctcgtcggg gctgcattcc aacggttact cgctggcccg caacgttctg ctggagatcg accggatgaa tctggccggt tatgtggagg agttcggccg cacgttgggt gaagagctat tggagcccac ccggatctat gccaaggact gtctggcgct caccgctgag acgcatgtcc gaacgttctg tcacgtcacg ggtggcgggt tggccggcaa cctggagcgg gtaatcccgc atgggctggt cgccgagatc gaccgcggca cctggacacc cgcgccggtg ttcgcgatga ttgcccagcg cggccgggtg gtgcgcgagg agatggaaaa gaccttcaac atgggcgtcg gcatgatcgc cgtcgtagcc cctgaagaca ccgaccgggc gctggccata ctgaccgcgc gccacctgga ctgctgggtg ctgggcaccg tctgcaaggg cggcaaagag ggcccacgtg ccacgctggt gggccagcac ccgcgaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.00768.a.A1.PS00855
PURIFICATION DATe: 11/9/2010
CONCENTRATION: 24.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Protocol Notes
notes unavailable
Validated AA Sequence
MTDPEKSPES SGRGPASQGV TYAAAGVDIE AGDRAVELFK PLATRATRPE VRGGLGGFAG LFALRNDYRE PVLAASTDGV GTKLAVAQAM DKHDTVGLDL VAMVVDDLVV CGAEPLFLQD YIAVGRTVPE RVSAIVSGIA EGCVRAGCAL LGGETAEHPG LMAPDHYDIS ATGVGVVEAD DVLGPERVKP GDVIIAMGSS GLHSNGYSLA RNVLLEIDRM NLAGYVEEFG RTLGEELLEP TRIYAKDCLA LTAETHVRTF CHVTGGGLAG NLERVIPHGL VAEIDRGTWT PAPVFAMIAQ RGRVVR
Validated NT Sequence
tcgcgcacca cccggccgcg ctgggcaatc atcgcgaaca ccggcgcggg tgtccaggtg ccgcggtcga tctcggcgac cagcccatgc gggattaccc gctccaggtt gccggccaac ccgccacccg tgacgtgaca gaacgttcgg acatgcgtct cagcggtgag cgccagacag tccttggcat agatccgggt gggctccaat agctcttcac ccaacgtgcg gccgaactcc tccacataac cggccagatt catccggtcg atctccagca gaacgttgcg ggccagcgag taaccgttgg aatgcagccc cgacgagccc atcgcgatga tgacgtcgcc gggtttgacg cgttccggcc ccagcacgtc gtcggcttcg accacaccca ccccggtggc cgagatgtcg taatggtcgg gtgccatcag cccgggatgt tcagcggtct caccccccag cagggcgcag ccagcgcgca cacacccctc cgcgatgccg ctgacgatcg cgctgacccg ttccggcacg gtccggccga ccgcgatgta gtcctgcagg aacagtggct ccgcgccaca gaccaccagg tcgtcgacca ccatcgcgac caggtcaaga ccgacagtgt cgtgcttgtc catcgcctgc gcgaccgcca gcttggtgcc gaccccgtcg gtggaggcgg ccagcaccgg ttcgcggtag tcgttgcgca gggcgaacaa tccggcaaat ccgccgagcc cgccgcgcac ctcgggtctg gtggcccggg ttgccaacgg cttgaacaac tccacggctc gatcgccggc ttcgatgtcc acacctgccg ccgcgtacgt gacgccttgg ctggctgggc ctcttccgga gctttctgga cttttttcgg gatccgtcat cgaaccagga ccctgggtct gagcttccag ggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDPEKSPE SSGRGPASQG VTYAAAGVDI EAGDRAVELF KPLATRATRP EVRGGLGGFA GLFALRNDYR EPVLAASTDG VGTKLAVAQA MDKHDTVGLD LVAMVVDDLV VCGAEPLFLQ DYIAVGRTVP ERVSAIVSGI AEGCVRAGCA LLGGETAEHP GLMAPDHYDI SATGVGVVEA DDVLGPERVK PGDVIIAMGS SGLHSNGYSL ARNVLLEIDR MNLAGYVEEF GRTLGEELLE PTRIYAKDCL ALTAETHVRT FCHVTGGGLA GNLERVIPHG LVAEIDRGTW TPAPVFAMIA QRGRVVREEM EKTFNMGVGM IAVVAPEDTD RALAILTARH LDCWVLGTVC KGGKEGPRAT LVGQHPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGATCCC GAAAAAAGTC CAGAAAGCTC CGGAAGAGGC CCAGCCAGCC AAGGCGTCAC GTACGCGGCG GCAGGTGTGG ACATCGAAGC CGGCGATCGA GCCGTGGAGT TGTTCAAGCC GTTGGCAACC CGGGCCACCA GACCCGAGGT GCGCGGCGGG CTCGGCGGAT TTGCCGGATT GTTCGCCCTG CGCAACGACT ACCGCGAACC GGTGCTGGCC GCCTCCACCG ACGGGGTCGG CACCAAGCTG GCGGTCGCGC AGGCGATGGA CAAGCACGAC ACTGTCGGTC TTGACCTGGT CGCGATGGTG GTCGACGACC TGGTGGTCTG TGGCGCGGAG CCACTGTTCC TGCAGGACTA CATCGCGGTC GGCCGGACCG TGCCGGAACG GGTCAGCGCG ATCGTCAGCG GCATCGCGGA GGGGTGTGTG CGCGCTGGCT GCGCCCTGCT GGGGGGTGAG ACCGCTGAAC ATCCCGGGCT GATGGCACCC GACCATTACG ACATCTCGGC CACCGGGGTG GGTGTGGTCG AAGCCGACGA CGTGCTGGGG CCGGAACGCG TCAAACCCGG CGACGTCATC ATCGCGATGG GCTCGTCGGG GCTGCATTCC AACGGTTACT CGCTGGCCCG CAACGTTCTG CTGGAGATCG ACCGGATGAA TCTGGCCGGT TATGTGGAGG AGTTCGGCCG CACGTTGGGT GAAGAGCTAT TGGAGCCCAC CCGGATCTAT GCCAAGGACT GTCTGGCGCT CACCGCTGAG ACGCATGTCC GAACGTTCTG TCACGTCACG GGTGGCGGGT TGGCCGGCAA CCTGGAGCGG GTAATCCCGC ATGGGCTGGT CGCCGAGATC GACCGCGGCA CCTGGACACC CGCGCCGGTG TTCGCGATGA TTGCCCAGCG CGGCCGGGTG GTGCGCGAGG AGATGGAAAA GACCTTCAAC ATGGGCGTCG GCATGATCGC CGTCGTAGCC CCTGAAGACA CCGACCGGGC GCTGGCCATA CTGACCGCGC GCCACCTGGA CTGCTGGGTG CTGGGCACCG TCTGCAAGGG CGGCAAAGAG GGCCCACGTG CCACGCTGGT GGGCCAGCAC CCGCGAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA