MyabA.00768.a

Phosphoribosylformylglycinamidine cyclo-ligase

CENTER ID: MyabA.00768.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00768.a.A1.GE30343 Full length( MyabA.00768.a ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00768.a.A1.PS00886 Full length( MyabA.00768.a ) 1 362

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.769
RefSeq: YP_001701478.1
UniProt: B1MI02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDMSERADK PSNSYAAAGV DIEAGDRAVE LFKKSVAKTH RPEVLGGIGG FAGLFALKGG YREPVLAAST DGVGTKIAVA QAMDKRDTVG LDLVAMVVDD LVVCGAEPLF LQDYIAVGKV VPERVSELVA GIAEGCAIAG CALLGGETAE HPGLMAPDDF DLSATGVGIV EADRVLGPDR VRPGDVVIAM GSSGLHSNGY SLARHVLLEI DRMDLFGQVE EFGRTLGEEL LEPTRIYAKD CLALAAETEV RTFCHVTGGG LAGNLARVIP DGLVAELDRG SWTPGPIFAM IAQRGRIERA EMEKTFNMGV GMVAVVAPED VDRALAVLTA RHIDCWTLGT IKKSGKDSGS ESALLVGQHP RF
NT Sequence
ATGCCCGATA TGAGCGAGCG AGCCGACAAA CCCAGTAATT CGTACGCCGC GGCCGGGGTG GACATCGAAG CCGGCGACCG GGCCGTCGAG CTGTTCAAGA AATCCGTCGC CAAGACGCAT CGTCCCGAGG TGTTGGGCGG TATCGGCGGC TTCGCCGGGC TGTTCGCGCT CAAGGGTGGT TACCGCGAGC CGGTGCTGGC CGCCTCCACC GACGGTGTCG GTACCAAGAT CGCCGTCGCA CAGGCCATGG ACAAACGCGA CACCGTCGGT CTGGACCTGG TGGCCATGGT TGTCGACGAC CTGGTCGTGT GCGGCGCCGA GCCGCTGTTC CTGCAGGATT ACATCGCGGT GGGCAAGGTG GTGCCCGAGC GTGTCAGCGA GCTGGTCGCC GGAATTGCCG AGGGGTGCGC CATCGCCGGC TGCGCACTGC TCGGCGGTGA GACTGCCGAA CATCCCGGGC TGATGGCTCC CGACGACTTC GATCTGTCGG CCACCGGCGT CGGCATCGTG GAAGCCGACC GCGTGCTGGG CCCGGATCGC GTGCGTCCGG GCGATGTCGT GATCGCCATG GGCTCGTCGG GCCTGCACTC CAACGGGTAC TCCCTGGCCC GGCACGTGCT GCTGGAGATC GATCGGATGG ATCTGTTCGG TCAGGTGGAA GAATTTGGCC GCACTCTGGG GGAGGAGCTG CTGGAACCCA CCCGGATCTA CGCCAAGGAC TGCCTGGCGC TGGCTGCCGA GACCGAGGTA CGGACTTTCT GCCACGTCAC CGGCGGCGGG CTCGCGGGCA ATCTGGCCCG CGTCATCCCC GATGGGCTCG TTGCCGAGCT CGACCGCGGC AGTTGGACCC CCGGCCCGAT CTTCGCGATG ATCGCCCAGC GCGGCCGCAT CGAGCGCGCC GAGATGGAGA AGACCTTCAA CATGGGCGTC GGCATGGTGG CCGTCGTCGC GCCCGAGGAT GTCGACAGGG CACTTGCGGT CCTGACGGCA CGGCACATCG ATTGCTGGAC CCTGGGCACC ATCAAGAAGT CGGGCAAGGA TTCAGGCAGC GAGAGCGCCC TACTCGTGGG ACAGCACCCG AGGTTC
Details for MyabA.00768.a.A1.GE30343
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MPDMSERADK PSNSYAAAGV DIEAGDRAVE LFKKSVAKTH RPEVLGGIGG FAGLFALKGG YREPVLAAST DGVGTKIAVA QAMDKRDTVG LDLVAMVVDD LVVCGAEPLF LQDYIAVGKV VPERVSELVA GIAEGCAIAG CALLGGETAE HPGLMAPDDF DLSATGVGIV EADRVLGPDR VRPGDVVIAM GSSGLHSNGY SLARHVLLEI DRMDLFGQVE EFGRTLGEEL LEPTRIYAKD CLALAAETEV RTFCHVTGGG LAGNLARVIP DGLVAELDRG SWTPGPIFAM IAQRGRIERA EMEKTFNMGV GMVAVVAPED VDRALAVLTA RHIDCWTLGT IKKSGKDSGS ESALLVGQHP RF
Validated NT Sequence
ttattagaac ctcgggtgct gtcccacgag tagggcgctc tcgctgcctg aatccttgcc cgacttcttg atggtgccca gggtccagca atcgatgtgc cgtgccgtca ggaccgcaag tgccctgtcg acatcctcgg gcgcgacgac ggccaccatg ccgacgccca tgttgaaggt cttctccatc tcggcgcgct cgatgcggcc gcgctgggcg atcatcgcga agatcgggcc gggggtccaa ctgccgcggt cgagctcggc aacgagccca tcggggatga cgcgggccag attgcccgcg agcccgccgc cggtgacgtg gcagaaagtc cgtacctcgg tctcggcagc cagcgccagg cagtccttgg cgtagatccg ggtgggttcc agcagctcct cccccagagt gcggccaaat tcttccacct gaccgaacag atccatccga tcgatctcca gcagcacgtg ccgggccagg gagtacccgt tggagtgcag gcccgacgag cccatggcga tcacgacatc gcccggacgc acgcgatccg ggcccagcac gcggtcggct tccacgatgc cgacgccggt ggccgacaga tcgaagtcgt cgggagccat cagcccggga tgttcggcag tctcaccgcc gagcagtgcg cagccggcga tggcgcaccc ctcggcaatt ccggcgacca gctcgctgac acgctcgggc accaccttgc ccaccgcgat gtaatcctgc aggaacagcg gctcggcgcc gcacacgacc aggtcgtcga caaccatggc caccaggtcc agaccgacgg tgtcgcgttt gtccatggcc tgtgcgacgg cgatcttggt accgacaccg tcggtggagg cggccagcac cggctcgcgg taaccaccct tgagcgcgaa cagcccggcg aagccgccga taccgcccaa cacctcggga cgatgcgtct tggcgacgga tttcttgaac agctcgacgg cccggtcgcc ggcttcgatg tccaccccgg ccgcggcgta cgaattactg ggtttgtcgg ctcgctcgct catatcgggc atcgaaccag gaccctgggt ctgagcttcc a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDMSERAD KPSNSYAAAG VDIEAGDRAV ELFKKSVAKT HRPEVLGGIG GFAGLFALKG GYREPVLAAS TDGVGTKIAV AQAMDKRDTV GLDLVAMVVD DLVVCGAEPL FLQDYIAVGK VVPERVSELV AGIAEGCAIA GCALLGGETA EHPGLMAPDD FDLSATGVGI VEADRVLGPD RVRPGDVVIA MGSSGLHSNG YSLARHVLLE IDRMDLFGQV EEFGRTLGEE LLEPTRIYAK DCLALAAETE VRTFCHVTGG GLAGNLARVI PDGLVAELDR GSWTPGPIFA MIAQRGRIER AEMEKTFNMG VGMVAVVAPE DVDRALAVLT ARHIDCWTLG TIKKSGKDSG SESALLVGQH PRF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgatatg agcgagcgag ccgacaaacc cagtaattcg tacgccgcgg ccggggtgga catcgaagcc ggcgaccggg ccgtcgagct gttcaagaaa tccgtcgcca agacgcatcg tcccgaggtg ttgggcggta tcggcggctt cgccgggctg ttcgcgctca agggtggtta ccgcgagccg gtgctggccg cctccaccga cggtgtcggt accaagatcg ccgtcgcaca ggccatggac aaacgcgaca ccgtcggtct ggacctggtg gccatggttg tcgacgacct ggtcgtgtgc ggcgccgagc cgctgttcct gcaggattac atcgcggtgg gcaaggtggt gcccgagcgt gtcagcgagc tggtcgccgg aattgccgag gggtgcgcca tcgccggctg cgcactgctc ggcggtgaga ctgccgaaca tcccgggctg atggctcccg acgacttcga tctgtcggcc accggcgtcg gcatcgtgga agccgaccgc gtgctgggcc cggatcgcgt gcgtccgggc gatgtcgtga tcgccatggg ctcgtcgggc ctgcactcca acgggtactc cctggcccgg cacgtgctgc tggagatcga tcggatggat ctgttcggtc aggtggaaga atttggccgc actctggggg aggagctgct ggaacccacc cggatctacg ccaaggactg cctggcgctg gctgccgaga ccgaggtacg gactttctgc cacgtcaccg gcggcgggct cgcgggcaat ctggcccgcg tcatccccga tgggctcgtt gccgagctcg accgcggcag ttggaccccc ggcccgatct tcgcgatgat cgcccagcgc ggccgcatcg agcgcgccga gatggagaag accttcaaca tgggcgtcgg catggtggcc gtcgtcgcgc ccgaggatgt cgacagggca cttgcggtcc tgacggcacg gcacatcgat tgctggaccc tgggcaccat caagaagtcg ggcaaggatt caggcagcga gagcgcccta ctcgtgggac agcacccgag gttctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.00768.a.A1.PS00886
PURIFICATION DATe: 12/2/2010
CONCENTRATION: 78.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MPDMSERADK PSNSYAAAGV DIEAGDRAVE LFKKSVAKTH RPEVLGGIGG FAGLFALKGG YREPVLAAST DGVGTKIAVA QAMDKRDTVG LDLVAMVVDD LVVCGAEPLF LQDYIAVGKV VPERVSELVA GIAEGCAIAG CALLGGETAE HPGLMAPDDF DLSATGVGIV EADRVLGPDR VRPGDVVIAM GSSGLHSNGY SLARHVLLEI DRMDLFGQVE EFGRTLGEEL LEPTRIYAKD CLALAAETEV RTFCHVTGGG LAGNLARVIP DGLVAELDRG SWTPGPIFAM IAQRGRIERA EMEKTFNMGV GMVAVVAPED VDRALAVLTA RHIDCWTLGT IKKSGKDSGS ESALLVGQHP RF
Validated NT Sequence
ttattagaac ctcgggtgct gtcccacgag tagggcgctc tcgctgcctg aatccttgcc cgacttcttg atggtgccca gggtccagca atcgatgtgc cgtgccgtca ggaccgcaag tgccctgtcg acatcctcgg gcgcgacgac ggccaccatg ccgacgccca tgttgaaggt cttctccatc tcggcgcgct cgatgcggcc gcgctgggcg atcatcgcga agatcgggcc gggggtccaa ctgccgcggt cgagctcggc aacgagccca tcggggatga cgcgggccag attgcccgcg agcccgccgc cggtgacgtg gcagaaagtc cgtacctcgg tctcggcagc cagcgccagg cagtccttgg cgtagatccg ggtgggttcc agcagctcct cccccagagt gcggccaaat tcttccacct gaccgaacag atccatccga tcgatctcca gcagcacgtg ccgggccagg gagtacccgt tggagtgcag gcccgacgag cccatggcga tcacgacatc gcccggacgc acgcgatccg ggcccagcac gcggtcggct tccacgatgc cgacgccggt ggccgacaga tcgaagtcgt cgggagccat cagcccggga tgttcggcag tctcaccgcc gagcagtgcg cagccggcga tggcgcaccc ctcggcaatt ccggcgacca gctcgctgac acgctcgggc accaccttgc ccaccgcgat gtaatcctgc aggaacagcg gctcggcgcc gcacacgacc aggtcgtcga caaccatggc caccaggtcc agaccgacgg tgtcgcgttt gtccatggcc tgtgcgacgg cgatcttggt accgacaccg tcggtggagg cggccagcac cggctcgcgg taaccaccct tgagcgcgaa cagcccggcg aagccgccga taccgcccaa cacctcggga cgatgcgtct tggcgacgga tttcttgaac agctcgacgg cccggtcgcc ggcttcgatg tccaccccgg ccgcggcgta cgaattactg ggtttgtcgg ctcgctcgct catatcgggc atcgaaccag gaccctgggt ctgagcttcc a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPDMSERAD KPSNSYAAAG VDIEAGDRAV ELFKKSVAKT HRPEVLGGIG GFAGLFALKG GYREPVLAAS TDGVGTKIAV AQAMDKRDTV GLDLVAMVVD DLVVCGAEPL FLQDYIAVGK VVPERVSELV AGIAEGCAIA GCALLGGETA EHPGLMAPDD FDLSATGVGI VEADRVLGPD RVRPGDVVIA MGSSGLHSNG YSLARHVLLE IDRMDLFGQV EEFGRTLGEE LLEPTRIYAK DCLALAAETE VRTFCHVTGG GLAGNLARVI PDGLVAELDR GSWTPGPIFA MIAQRGRIER AEMEKTFNMG VGMVAVVAPE DVDRALAVLT ARHIDCWTLG TIKKSGKDSG SESALLVGQH PRF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGATATG AGCGAGCGAG CCGACAAACC CAGTAATTCG TACGCCGCGG CCGGGGTGGA CATCGAAGCC GGCGACCGGG CCGTCGAGCT GTTCAAGAAA TCCGTCGCCA AGACGCATCG TCCCGAGGTG TTGGGCGGTA TCGGCGGCTT CGCCGGGCTG TTCGCGCTCA AGGGTGGTTA CCGCGAGCCG GTGCTGGCCG CCTCCACCGA CGGTGTCGGT ACCAAGATCG CCGTCGCACA GGCCATGGAC AAACGCGACA CCGTCGGTCT GGACCTGGTG GCCATGGTTG TCGACGACCT GGTCGTGTGC GGCGCCGAGC CGCTGTTCCT GCAGGATTAC ATCGCGGTGG GCAAGGTGGT GCCCGAGCGT GTCAGCGAGC TGGTCGCCGG AATTGCCGAG GGGTGCGCCA TCGCCGGCTG CGCACTGCTC GGCGGTGAGA CTGCCGAACA TCCCGGGCTG ATGGCTCCCG ACGACTTCGA TCTGTCGGCC ACCGGCGTCG GCATCGTGGA AGCCGACCGC GTGCTGGGCC CGGATCGCGT GCGTCCGGGC GATGTCGTGA TCGCCATGGG CTCGTCGGGC CTGCACTCCA ACGGGTACTC CCTGGCCCGG CACGTGCTGC TGGAGATCGA TCGGATGGAT CTGTTCGGTC AGGTGGAAGA ATTTGGCCGC ACTCTGGGGG AGGAGCTGCT GGAACCCACC CGGATCTACG CCAAGGACTG CCTGGCGCTG GCTGCCGAGA CCGAGGTACG GACTTTCTGC CACGTCACCG GCGGCGGGCT CGCGGGCAAT CTGGCCCGCG TCATCCCCGA TGGGCTCGTT GCCGAGCTCG ACCGCGGCAG TTGGACCCCC GGCCCGATCT TCGCGATGAT CGCCCAGCGC GGCCGCATCG AGCGCGCCGA GATGGAGAAG ACCTTCAACA TGGGCGTCGG CATGGTGGCC GTCGTCGCGC CCGAGGATGT CGACAGGGCA CTTGCGGTCC TGACGGCACG GCACATCGAT TGCTGGACCC TGGGCACCAT CAAGAAGTCG GGCAAGGATT CAGGCAGCGA GAGCGCCCTA CTCGTGGGAC AGCACCCGAG GTTCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA