MysmA.00768.a

Phosphoribosylformylglycinamidine cyclo-ligase

CENTER ID: MysmA.00768.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00768.a.A1.GE28839 Full length( MysmA.00768.a ) 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00768.a.A1.PS00620 Full length( MysmA.00768.a ) 1 360

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5642
RefSeq: YP_890026.1
UniProt: A0R4D8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDREDSVSS DAISYASAGV DIEAGDRAVE LFKPLAAKAT RPEVRGGLGG FAGLFALKRD YREPVLASST DGVGTKLAIA QAMDKHDTVG LDLVAMVVDD LVVCGAEPLF LQDYIAVGRT VPERVSAIVA GIAEGCVRAG CALLGGETAE HPGLMEPDHY DISATGVGIV EADDVLGPDR VRPGDVIIAM ASSGLHSNGY SLARKVLLEI DRMNLAGHVE EFGRTLGEEL LEPTLIYAKD CLALAAETQV RTFCHVTGGG LAGNLERVIP HGLTAELDRG TWTPAPVFGM IAQRGRIERA EMEKTFNMGV GMVAVVAPED TDRALAILTA RHLNCWTLGT VKKGGKDSVR AQLVGQHPRF
NT Sequence
ATGACCGATC GCGAAGACTC CGTTTCCAGT GATGCAATCT CCTACGCGTC GGCCGGGGTC GACATCGAAG CTGGCGATCG CGCCGTGGAG TTGTTCAAGC CTCTGGCCGC CAAGGCCACC CGCCCTGAGG TCCGTGGCGG CCTCGGCGGT TTCGCGGGCC TGTTCGCCCT CAAGCGTGAT TATCGAGAGC CGGTGCTGGC CTCGTCCACA GACGGTGTCG GCACCAAACT GGCGATAGCG CAGGCGATGG ACAAGCACGA CACCGTCGGC CTCGACCTGG TCGCGATGGT GGTCGACGAC CTCGTGGTGT GCGGCGCCGA ACCGTTGTTC CTGCAGGACT ACATCGCCGT CGGGCGCACC GTGCCCGAGC GCGTGAGTGC CATCGTGGCA GGCATCGCCG AGGGCTGTGT GCGGGCCGGA TGCGCGCTGC TGGGCGGCGA GACCGCCGAG CACCCGGGGC TGATGGAACC GGACCACTAC GACATCTCCG CGACCGGTGT GGGCATCGTG GAGGCCGACG ACGTGCTGGG CCCCGACCGG GTCAGGCCGG GCGACGTCAT CATCGCCATG GCTTCGAGCG GTCTGCACTC CAACGGCTAC TCGTTGGCCC GCAAGGTCCT GCTGGAGATC GACCGGATGA ACCTCGCCGG CCACGTCGAG GAGTTCGGCC GCACGCTCGG TGAGGAGCTG CTCGAACCCA CCCTGATCTA CGCCAAGGAC TGCCTGGCGC TCGCCGCCGA GACCCAGGTG CGAACCTTCT GCCACGTCAC CGGTGGTGGC CTGGCGGGCA ACCTCGAACG CGTCATCCCG CACGGCCTCA CCGCCGAACT GGACCGCGGC ACGTGGACCC CGGCGCCGGT GTTCGGCATG ATCGCTCAGC GCGGACGGAT CGAACGCGCC GAGATGGAGA AGACGTTCAA CATGGGTGTC GGCATGGTCG CCGTCGTCGC TCCCGAGGAC ACCGATCGTG CGCTGGCCAT ACTGACCGCT CGGCATCTGA ATTGCTGGAC GCTGGGCACG GTCAAGAAGG GCGGCAAGGA CAGTGTGCGC GCCCAGTTGG TCGGCCAGCA CCCACGGTTC
Details for MysmA.00768.a.A1.GE28839
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDREDSVS SDAISYASAG VDIEAGDRAV ELFKPLAAKA TRPEVRGGLG GFAGLFALKR DYREPVLASS TDGVGTKLAI AQAMDKHDTV GLDLVAMVVD DLVVCGAEPL FLQDYIAVGR TVPERVSAIV AGIAEGCVRA GCALLGGETA EHPGLMEPDH YDISATGVGI VEADDVLGPD RVRPGDVIIA MASSGLHSNG YSLARKVLLE IDRMNLAGHV EEFGRTLGEE LLEPTLIYAK DCLALAAETQ VRTFCHVTGG GLAGNLERVI PHGLTAELDR GTWTPAPVFG MIAQRGRIER AEMEKTFNMG VGMVAVVAPE DTDRALAILT ARHLNCWTLG TVKKGGKDSV RAQLVGQHPR F
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg atcgcgaaga ctccgtttcc agtgatgcaa tctcctacgc gtcggccggg gtcgacatcg aagctggcga tcgcgccgtg gagttgttca agcctctggc cgccaaggcc acccgccctg aggtccgtgg cggcctcggc ggtttcgcgg gcctgttcgc cctcaagcgt gattatcgag agccggtgct ggcctcgtcc acagacggtg tcggcaccaa actggcgata gcgcaggcga tggacaagca cgacaccgtc ggcctcgacc tggtcgcgat ggtggtcgac gacctcgtgg tgtgcggcgc cgaaccgttg ttcctgcagg actacatcgc cgtcgggcgc accgtgcccg agcgcgtgag tgccatcgtg gcaggcatcg ccgagggctg tgtgcgggcc ggatgcgcgc tgctgggcgg cgagaccgcc gagcacccgg ggctgatgga accggaccac tacgacatct ccgcgaccgg tgtgggcatc gtggaggccg acgacgtgct gggccccgac cgggtcaggc cgggcgacgt catcatcgcc atggcttcga gcggtctgca ctccaacggc tactcgttgg cccgcaaggt cctgctggag atcgaccgga tgaacctcgc cggccacgtc gaggagttcg gccgcacgct cggtgaggag ctgctcgaac ccaccctgat ctacgccaag gactgcctgg cgctcgccgc cgagacccag gtgcgaacct tctgccacgt caccggtggt ggcctggcgg gcaacctcga acgcgtcatc ccgcacggcc tcaccgccga actggaccgc ggcacgtgga ccccggcgcc ggtgttcggc atgatcgctc agcgcggacg gatcgaacgc gccgagatgg agaagacgtt caacatgggt gtcggcatgg tcgccgtcgt cgctcccgag gacaccgatc gtgcgctggc catactgacc gctcggcatc tgaattgctg gacgctgggc acggtcaaga agggcggcaa ggacagtgtg cgcgcccagt tggtcggcca gcacccacgg ttcta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDREDSVS SDAISYASAG VDIEAGDRAV ELFKPLAAKA TRPEVRGGLG GFAGLFALKR DYREPVLASS TDGVGTKLAI AQAMDKHDTV GLDLVAMVVD DLVVCGAEPL FLQDYIAVGR TVPERVSAIV AGIAEGCVRA GCALLGGETA EHPGLMEPDH YDISATGVGI VEADDVLGPD RVRPGDVIIA MASSGLHSNG YSLARKVLLE IDRMNLAGHV EEFGRTLGEE LLEPTLIYAK DCLALAAETQ VRTFCHVTGG GLAGNLERVI PHGLTAELDR GTWTPAPVFG MIAQRGRIER AEMEKTFNMG VGMVAVVAPE DTDRALAILT ARHLNCWTLG TVKKGGKDSV RAQLVGQHPR F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgatcgc gaagactccg tttccagtga tgcaatctcc tacgcgtcgg ccggggtcga catcgaagct ggcgatcgcg ccgtggagtt gttcaagcct ctggccgcca aggccacccg ccctgaggtc cgtggcggcc tcggcggttt cgcgggcctg ttcgccctca agcgtgatta tcgagagccg gtgctggcct cgtccacaga cggtgtcggc accaaactgg cgatagcgca ggcgatggac aagcacgaca ccgtcggcct cgacctggtc gcgatggtgg tcgacgacct cgtggtgtgc ggcgccgaac cgttgttcct gcaggactac atcgccgtcg ggcgcaccgt gcccgagcgc gtgagtgcca tcgtggcagg catcgccgag ggctgtgtgc gggccggatg cgcgctgctg ggcggcgaga ccgccgagca cccggggctg atggaaccgg accactacga catctccgcg accggtgtgg gcatcgtgga ggccgacgac gtgctgggcc ccgaccgggt caggccgggc gacgtcatca tcgccatggc ttcgagcggt ctgcactcca acggctactc gttggcccgc aaggtcctgc tggagatcga ccggatgaac ctcgccggcc acgtcgagga gttcggccgc acgctcggtg aggagctgct cgaacccacc ctgatctacg ccaaggactg cctggcgctc gccgccgaga cccaggtgcg aaccttctgc cacgtcaccg gtggtggcct ggcgggcaac ctcgaacgcg tcatcccgca cggcctcacc gccgaactgg accgcggcac gtggaccccg gcgccggtgt tcggcatgat cgctcagcgc ggacggatcg aacgcgccga gatggagaag acgttcaaca tgggtgtcgg catggtcgcc gtcgtcgctc ccgaggacac cgatcgtgcg ctggccatac tgaccgctcg gcatctgaat tgctggacgc tgggcacggt caagaagggc ggcaaggaca gtgtgcgcgc ccagttggtc ggccagcacc cacggttcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.00768.a.A1.PS00620
PURIFICATION DATe: 5/27/2010
CONCENTRATION: 24.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDREDSVS SDAISYASAG VDIEAGDRAV ELFKPLAAKA TRPEVRGGLG GFAGLFALKR DYREPVLASS TDGVGTKLAI AQAMDKHDTV GLDLVAMVVD DLVVCGAEPL FLQDYIAVGR TVPERVSAIV AGIAEGCVRA GCALLGGETA EHPGLMEPDH YDISATGVGI VEADDVLGPD RVRPGDVIIA MASSGLHSNG YSLARKVLLE IDRMNLAGHV EEFGRTLGEE LLEPTLIYAK DCLALAAETQ VRTFCHVTGG GLAGNLERVI PHGLTAELDR GTWTPAPVFG MIAQRGRIER AEMEKTFNMG VGMVAVVAPE DTDRALAILT ARHLNCWTLG TVKKGGKDSV RAQLVGQHPR F
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg atcgcgaaga ctccgtttcc agtgatgcaa tctcctacgc gtcggccggg gtcgacatcg aagctggcga tcgcgccgtg gagttgttca agcctctggc cgccaaggcc acccgccctg aggtccgtgg cggcctcggc ggtttcgcgg gcctgttcgc cctcaagcgt gattatcgag agccggtgct ggcctcgtcc acagacggtg tcggcaccaa actggcgata gcgcaggcga tggacaagca cgacaccgtc ggcctcgacc tggtcgcgat ggtggtcgac gacctcgtgg tgtgcggcgc cgaaccgttg ttcctgcagg actacatcgc cgtcgggcgc accgtgcccg agcgcgtgag tgccatcgtg gcaggcatcg ccgagggctg tgtgcgggcc ggatgcgcgc tgctgggcgg cgagaccgcc gagcacccgg ggctgatgga accggaccac tacgacatct ccgcgaccgg tgtgggcatc gtggaggccg acgacgtgct gggccccgac cgggtcaggc cgggcgacgt catcatcgcc atggcttcga gcggtctgca ctccaacggc tactcgttgg cccgcaaggt cctgctggag atcgaccgga tgaacctcgc cggccacgtc gaggagttcg gccgcacgct cggtgaggag ctgctcgaac ccaccctgat ctacgccaag gactgcctgg cgctcgccgc cgagacccag gtgcgaacct tctgccacgt caccggtggt ggcctggcgg gcaacctcga acgcgtcatc ccgcacggcc tcaccgccga actggaccgc ggcacgtgga ccccggcgcc ggtgttcggc atgatcgctc agcgcggacg gatcgaacgc gccgagatgg agaagacgtt caacatgggt gtcggcatgg tcgccgtcgt cgctcccgag gacaccgatc gtgcgctggc catactgacc gctcggcatc tgaattgctg gacgctgggc acggtcaaga agggcggcaa ggacagtgtg cgcgcccagt tggtcggcca gcacccacgg ttcta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDREDSVS SDAISYASAG VDIEAGDRAV ELFKPLAAKA TRPEVRGGLG GFAGLFALKR DYREPVLASS TDGVGTKLAI AQAMDKHDTV GLDLVAMVVD DLVVCGAEPL FLQDYIAVGR TVPERVSAIV AGIAEGCVRA GCALLGGETA EHPGLMEPDH YDISATGVGI VEADDVLGPD RVRPGDVIIA MASSGLHSNG YSLARKVLLE IDRMNLAGHV EEFGRTLGEE LLEPTLIYAK DCLALAAETQ VRTFCHVTGG GLAGNLERVI PHGLTAELDR GTWTPAPVFG MIAQRGRIER AEMEKTFNMG VGMVAVVAPE DTDRALAILT ARHLNCWTLG TVKKGGKDSV RAQLVGQHPR F
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGATCGC GAAGACTCCG TTTCCAGTGA TGCAATCTCC TACGCGTCGG CCGGGGTCGA CATCGAAGCT GGCGATCGCG CCGTGGAGTT GTTCAAGCCT CTGGCCGCCA AGGCCACCCG CCCTGAGGTC CGTGGCGGCC TCGGCGGTTT CGCGGGCCTG TTCGCCCTCA AGCGTGATTA TCGAGAGCCG GTGCTGGCCT CGTCCACAGA CGGTGTCGGC ACCAAACTGG CGATAGCGCA GGCGATGGAC AAGCACGACA CCGTCGGCCT CGACCTGGTC GCGATGGTGG TCGACGACCT CGTGGTGTGC GGCGCCGAAC CGTTGTTCCT GCAGGACTAC ATCGCCGTCG GGCGCACCGT GCCCGAGCGC GTGAGTGCCA TCGTGGCAGG CATCGCCGAG GGCTGTGTGC GGGCCGGATG CGCGCTGCTG GGCGGCGAGA CCGCCGAGCA CCCGGGGCTG ATGGAACCGG ACCACTACGA CATCTCCGCG ACCGGTGTGG GCATCGTGGA GGCCGACGAC GTGCTGGGCC CCGACCGGGT CAGGCCGGGC GACGTCATCA TCGCCATGGC TTCGAGCGGT CTGCACTCCA ACGGCTACTC GTTGGCCCGC AAGGTCCTGC TGGAGATCGA CCGGATGAAC CTCGCCGGCC ACGTCGAGGA GTTCGGCCGC ACGCTCGGTG AGGAGCTGCT CGAACCCACC CTGATCTACG CCAAGGACTG CCTGGCGCTC GCCGCCGAGA CCCAGGTGCG AACCTTCTGC CACGTCACCG GTGGTGGCCT GGCGGGCAAC CTCGAACGCG TCATCCCGCA CGGCCTCACC GCCGAACTGG ACCGCGGCAC GTGGACCCCG GCGCCGGTGT TCGGCATGAT CGCTCAGCGC GGACGGATCG AACGCGCCGA GATGGAGAAG ACGTTCAACA TGGGTGTCGG CATGGTCGCC GTCGTCGCTC CCGAGGACAC CGATCGTGCG CTGGCCATAC TGACCGCTCG GCATCTGAAT TGCTGGACGC TGGGCACGGT CAAGAAGGGC GGCAAGGACA GTGTGCGCGC CCAGTTGGTC GGCCAGCACC CACGGTTCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA